Evidence of Elevated Ozone Concentrations on Forested Slopes of the Lower Fraser Valley, British Columbia, Canada

During the summers of 2001 and 2002, hourly average ozone concentrations were measured at three sites of differing elevation (188, 588, and 1221 m.a.s.l.) on the forested south-facing slopes of the Lower Fraser Valley (LFV), British Columbia. Sites experienced ozone concentrations ranging from 0 to 88 ppb in 2001, and 0 to 96 ppb in 2002. Daily patterns were in agreement with previous studies showing morning increases and late afternoon peaks. Reduced diurnal variation increased the exposure of higher-elevation forested sites. An upper-level ridge coinciding with a thermal coastal trough caused above-average ozone concentrations, and the ‘maximum acceptable’ 1-hour National Ambient Air Quality Objective (AQO) of 82 ppb to be exceeded. Maximum ozone concentrations and AQO exceedance frequency both increased with distance eastward in the valley. A preliminary survey of ozone-like injury symptoms on native shrubs suggested that the elevated ozone levels occurring in the LFV may cause injury to forest plants.     

Sewage Treatment in a Rotating Biological Contactor (RBC) System

The treatment of domestic wastewater at a temperature of 12–24°C was investigated in an RBC system. The RBC consists of a two stage system connected in series. The system was operated at different organic loading rates (OLR's) and hydraulic retntion times (HRT's) in order to optimize the RBC performance. The overall removal efficiencies for chemical oxygen demand (COD_total, COD_suspended and COD_colloidal) significantly decreased when decreasing the total HRT from 10 to 2.5 h and increasing the OLR from 11 to 47 g COD/m².d. However, the effluent quality of COD_soluble remained unaffected. Most of the COD was removed in the 1^st stage and nitrification took place in the 2^nd stage of the two stage system.The overall nitrification efficiency was 49% at total OLR of 11 gCOD/m².d. At total HRT's of 10, 5 and 2.5 h, the Escherchia coli (E. coli) concentration was reduced by a value of 1.6, 1.5 and 0.8 log₁₀ respectively. The sludge volume index (SVI) decreased as the OLR increased. However, the SVI of the excess sludge produced in the RBC under different OLR's was always <74 ml/gTS, which indicates vadjust a good settleability. The performance of the single versus two stage RBC operated at the same total OLR of 22 g COD/m².d and the same HRT of 5.0 h was examined. The results obtained showed that the COD concentration and the E. coli content in the final effluent of a two stage were lower than in the effluent of the single stage RBC. Moreover, the nitrification efficiency in the two stage system was higher comapred to one stage system.     

Structure determination of partially deuterated carotenoids from intrinsically labeled vegetables by HPLC-MS and 1H NMR

The structures of biosynthetic deuterated carotenoids in labeled vegetables were investigated: (all-E)-lutein and (all-E)-beta-carotene from spinach, and (all-E)-beta-carotene and (all-E)-alpha-carotene from carrots. The vegetables were grown hydroponically using a nutrient solution enriched with deuterium oxide (D(2)O) and were extracted using matrix solid-phase dispersion (MSPD). Deuterium enrichment in the carotenoid molecules was determined by liquid chromatography-mass spectrometry (LC-MS). (all-E)-Lutein and (all-E)-beta-carotene in spinach showed partial deuteration from (2)H(1) to (2)H(12), with the abundance maximum at (2)H(5). (all-E)-beta-Carotene and (all-E)-alpha-carotene from carrots showed partial deuteration from (2)H(1) to (2)H(17), with the abundance maximum at (2)H(11). The (1)H NMR spectra of the four deuterated carotenoids showed additional signals for all methyl groups and decreased signal intensity for the olefinic protons and the methylene protons in the ring. These differences are due to isotopic effects and are based on the substitution of protons by deuterium atoms. The deuteration was distributed randomly throughout the carotenoid molecules.     

Antibacterial and antioxidant activities of essential oils isolated from Thymbra capitata L. (Cav.) andOriganum vulgare L

Antilisterial activities of Thymbra capitata and Origanum vulgare essential oils were tested against 41 strains of Listeria monocytogenes. The oil of T. capitata was mainly constituted by one component, carvacrol (79%), whereas for O. vulgare three components constituted 70% of the oil, namely, thymol (33%), gamma-terpinene (26%), and p-cymene (11%). T. capitata essential oil had a significantly higher antilisterial activity in comparison to O. vulgare oil and chloramphenicol. No significant differences in L. monocytogenes susceptibilities to the essential oils tested were registered. The minimum inhibitory concentration values of T. capitata essential oil and of carvacrol were quite similar, ranging between 0.05 and 0.2 microL/mL. Antioxidant activity was also tested, the essential oil of T. capitata showing significantly higher antioxidant activity than that of O. vulgare. Use of T. capitata and O. vulgare essential oils can constitute a powerful tool in the control of L. monocytogenes in food and other industries.     

Time-resolved spectral studies of blue-green fluorescence of artichoke (Cynara cardunculus L. Var. Scolymus) leaves: identification of chlorogenic acid as one of the major fluorophores and age-mediated changes

Synchrotron radiation and the time-correlated single-photon counting technique were used to investigate the spectral and time-resolved characteristics of blue-green fluorescence (BGF) of artichoke leaves. Leaves emitted BGF under ultraviolet (UV) excitation; the abaxial side was much more fluorescent than the adaxial side, and in both cases, the youngest leaves were much more fluorescent than the oldest ones. The BGF of artichoke leaves was dominated by the presence of hydroxycinnamic acids. A decrease in the percentage of BGF attributable to the very short kinetic component (from 42 to 20%), in the shape of the BGF excitation spectra, and chlorogenic acid concentrations indicate that there is a loss of hydroxycinnamic acid with leaf age. Studies on excitation, emission, and synchronized fluorescence spectra of leaves and trichomes and chlorogenic acid contents indicate that chlorogenic acid is one of the main blue-green fluorophores in artichoke leaves. Results of the present study indicate that 20-42% (i.e., the very short kinetic component) of the overall BGF is emitted by chlorogenic acid. Time-resolved BGF measurements could be a means to extract information on chlorogenic acid fluorescence from the overall leaf BGF.     

Artificial neural networks for estimating soil hydraulic parameters from dynamic flow experiments

Inverse methods are often used for estimating soil hydraulic parameters from experiments on flow of water through soil. We propose here an alternative method using neural networks. We teach a problem‐adapted network of radial basis functions (RBF) the relationship between soil parameters and transient flow patterns using a numerical flow model. The trained RBF network accurately identifies soil parameters from flow patterns not contained in the training scenarios. A comparison with the inverse method (Annealing‐Simplex) reveals a similarly good prediction by both approaches for randomly perturbed data and data from the real world. Nonetheless, the inverse method showed dependency on initial parameter estimates not required by the RBF network. Training demands moderately more computation and manpower than the inverse technique, but the absolutely stable and simple network application requires negligible resources. Thus, for individual applications, the network approach is slightly surpassed by the Annealing‐Simplex method. However, the RBF network has to be trained only once and, subsequently, it can be applied easily and without effort upon any number of laboratory experiments with standardized experimental setups.     

Assessment of tillage translocation and tillage erosion by hoeing on the steep land in hilly areas of Sichuan, China

Most of the tillage erosion studies have focused on the effect of tractor-plough tillage on soil translocation and soil loss. Only recently, have a few studies contributed to the understanding of tillage erosion by manual tillage. Furthermore, little is known about the impact of tillage erosion in hilly areas of the humid sub-tropics. This study on tillage erosion by hoeing was conducted on a purple soil (Regosols) of the steep land, in Jianyang County, Sichuan Province, southwestern China (30°24′N and 104°35′E) using the physical tracer method.

The effects of hoeing tillage on soil translocation on hillslopes are quite evident. The tillage transport coefficients were 26–38 kg m⁻¹ per tillage pass and 121–175 kg m⁻¹ per tillage pass respectively for k₃- and k₄-values. Given that there was a typical downslope parcel length of 15 m and two times of tillage per year in this area, the tillage erosion rates on the 4–43% hillslopes reached 48–151 Mg ha⁻¹ per year. The downslope soil translocation is closely related to slope gradient. Lateral soil translocation by such tillage is also obvious though it is lower than downslope soil translocation. Strong downslope translocation accounts for thin soil layers and the exposure of parent materials/rocks at the ridge tops and on convexities in the hilly areas. Deterioration in soil quality and therefore reduction in plant productivity due to tillage-induced erosion would be evident at the ridge tops and convex shoulders.     

Speciation of arsenic in different types of nuts by ion chromatography-inductively coupled plasma mass spectrometry

In this work the quantitative determination and analytical speciation of arsenic were undertaken in different types of nuts, randomly purchased from local markets. The hardness of the whole nuts and high lipid content made the preparation of this material difficult for analysis. The lack of sample homogeneity caused irreproducible results. To improve the precision of analysis, arsenic was determined separately in nut oil and in the defatted sample. The lipids were extracted from the ground sample with the two portions of a mixture of chloroform and methanol (2:1). The defatted material was dried and ground again, yielding a fine powder. The nut oil was obtained by combining the two organic extracts and by evaporating the solvents. The two nut fractions were microwave digested, and total arsenic was determined by inductively coupled plasma mass spectrometry (ICP-MS). The results obtained for oils from different types of nuts showed element concentration in the range 2.9-16.9 ng g(-)(1). Lower levels of arsenic were found in defatted material (<0.1 ng g(-)(1) with the exception of Brazil nuts purchased with and without shells, 3.0 and 2.8 ng g(-)(1) respectively). For speciation analysis of arsenic in nut oils, elemental species were extracted from 2 g of oil with 12 mL of chloroform/methanol (2:1) and 8 mL of deionized water. The aqueous layer, containing polar arsenic species, was evaporated and the residue dissolved and analyzed by ion chromatography-ICP-MS. The anion exchange chromatography enabled separation of As(III), dimethylarsinic acid (DMAs(V)), monomethylarsonic acid (MMAs(V)), and As(V) within 8 min. Several types of nuts were analyzed, including walnuts, Brazil nuts, almonds, cashews, pine nuts, peanuts, pistachio nuts, and sunflower seeds. The recovery for the speciation procedure was in the range 72.7-90.6%. The primary species found in the oil extracts were As(III) and As(V). The arsenic concentration levels in these two species were 0.7-12.7 and 0.5-4.3 ng g(-)(1), respectively. The contribution of As in DMAs(V) ranged from 0.1 +/- 0.1 ng g(-)(1) in walnuts to 1.3 +/- 0.3 ng g(-)(1) in pine nuts. MMAs(V) was not detected in almonds, peanuts, pine nuts, sunflower seeds, or walnuts, and the highest concentration was found in pistachio nuts (0.5 +/- 0.2 ng g(-)(1)).     

Analysis of virgin olive oil volatiles by a novel electronic nose based on a miniaturized SAW sensor array coupled with SPME enhanced headspace enrichment

A novel electronic nose based on solid-phase microextraction (SPME) coupled with a surface acoustic wave (SAW) sensor array has been used to analyze different quality virgin olive oils. A mathematical model was designed with 37 samples to distinguish lampante from the other virgin olive oils categories (extra-virgin and virgin), because lampante-virgin olive oils cannot be consumed without a previous refining process. The model, successfully validated with a test set of 16 samples, was able to classify 90% of the samples correctly. Misclassifications were explained by SPME-HRGC analyses and a second sensory evaluation.     

Hydrolase activity, microbial biomass and community structure in long-term Cd-contaminated soils

Long-term effects of high Cd concentrations on enzyme activities, microbial biomass and respiration and bacterial community structure of soils were assessed in sandy soils where Cd was added between 1988 and 1990 as Cd(NO₃)₂ to reach concentrations ranging from 0 to 0.36 mmol Cd kg⁻¹ dry weight soil. Soils were mantained under maize and grass cultivation, or ‘set-aside’ regimes, for 1 year. Solubility of Cd and its bioavailability were measured by chemical extractions or by the BIOMET bacterial biosensor system. Cadmium solubility was very low, and Cd bioavailability was barely detectable even in soils polluted with 0.36 mmol Cd kg⁻¹. Soil microbial biomass carbon (B_C) was slightly decreased and respiration was increased significantly even at the lower Cd concentration and as a consequence the metabolic quotient (qCO₂) was increased, indicating a stressful condition for soil microflora. However, Cd-contaminated soils also had a lower total organic C (TOC) content and thus the microbial biomass C-to-TOC ratio was unaffected by Cd. Alkaline phosphomonoesterase, arylsulphatase and protease activities were significantly reduced in all Cd-contaminated soils whereas acid phosphomonoesterase, β-glucosidase and urease activites were unaffected by Cd. Neither changes in physiological groups of bacteria, nor of Cd resistant bacteria could be detected in numbers of the culturable bacterial community. Denaturing gradient gel electrophoresis analysis of the bacterial community showed slight changes in maize cropped soils containing 0.18 and 0.36 mmol Cd kg⁻¹ soil as compared to the control. It was concluded that high Cd concentrations induced mainly physiological adaptations rather than selection for metal-resistant culturable soil microflora, regardless of Cd concentration, and that some biochemical parameters were more sensitive to stress than others.