陕北地区秦川牛及其多个杂交后代群体的遗传多样性

为了解陕北地区黄牛的遗传多样性及遗传背景,采用直接测序技术对55头饲养在陕北榆林地区的秦川牛及其多个杂交后代群体的线粒体DNA D-loop区826bp序列进行测定。结果表明,榆林地区黄牛D-loop区序列A+T平均含量为61.5%,G+C含量38.5%;共检测到33种单倍型和84个变异位点,核苷酸多样度(π)为0.021 43,单倍型多样度(h)为0.970,表明陕北榆林地区的黄牛具有丰富的遗传多样性。NJ法聚类结果显示,该地区的黄牛品种或群体有2个母系起源。     

Application of Function Analysis in Conceptual Design

Product design is a procedure that enterprises meet the needs of consumers; conceptive design is one of the steps in product design and it directly considers requirements of consumers. So how to supply consumers with satisfying function is the essential point. On the basis of function analysis of Value Engineering, the paper puts forward a new method to exercise function definition and function analysis, implements conceptual product design procedure with database techniques.A new way is gotten to convert requirements to conceptual product.     

A Product BOM Management Method Based on Single Layer BOM

Bill of material of product is data about product structure, and plays an important role in product information management. The paper analyzed characteristics of product BOM, and put forward a product BOM management method based on single layer BOM, witch can well meet requirements of product BOM.     

小麦 Glu A1位点1亚基和Null亚基间品质效应的差异

为了解小麦Glu-A1位点1亚基和Null亚基间的品质效应差异,用郑麦9405/郑麦98//周麦16配制杂交组合,利用系谱法辅助生化标记连续多代选择后得到纯合的1亚基和Null亚基的近等基因系,并对近等基因系1亚基和Null亚基间的面筋特性和粉质特性进行了测定和分析.结果表明,对于面筋指数、形成时间、稳定时间和粉质质量指数,1亚基的贡献明显大于Null亚基,1亚基比Null亚基的面筋指数、形成时间、稳定时间和粉质质量指数分别提高9.79％、68.73％、25.89％和13.99％,成组数据的t测验显示差异达到极显著水平(P值分别为0.007、0.000、0.002和0.006),亚基效应为1亚基＞Null亚基;对于湿面筋含量、干面筋含量和吸水率,1亚基和Null亚基的贡献无明显差别,成组数据t检验显示差异不显著(0.978、0.654和0.206).     

高产小麦品种蘖叶构型动态模式的探索

为阐述高产的小麦蘖叶构型特征,并验证了具有这种蘖叶构型小麦品种的高产性,对郑麦7698、周麦18和矮抗58的蘖叶动态及其特征进行分析。结果表明,高产小麦蘖叶建构过程中,分蘖以冬前低位蘖为主,春生高位蘖少且不拔节;小麦生育前期冠层上部叶片直立,冠层透光性好,下部叶片面积大且持绿期长,在灌浆中后期叶片仍能保持较好的直立性。这种蘖叶构型分蘖冗余小,光和养分资源能高效利用,同时冠层透光性好,群体光合同化能力可以得到充分发挥,进而易于实现高产。该蘖叶构型在密植条件下的优势更为突出,有助于解决生产中农民大播量种植进而造成倒伏减产的问题。     

低糖果味胡萝卜脯加工工艺的研究

以新鲜胡萝卜为原料,制作低糖果味胡萝卜果脯。在传统生产工艺中增加了缓冻预处理工序,果脯加工工艺的优化结果为:在-18℃下冻结6h,不仅能较好地促进渗糖,而且口感较佳;渗糖液添加组分为:25%的蔗糖,25%的淀粉糖浆,0.4%的柠檬酸,1.2%的鲜橘皮和0.4%的明胶;采用60～65℃恒温鼓风干燥6～7h,以1.0%的海藻酸钠胶体浸泡1min后继续干燥1～2h,制得成品的色、香、味、形俱佳。     

坝上地区景观格局动态变化分析

在遥感与G IS技术支持下,利用景观格局分析软件Fragstats分别计算了研究区内1987、1999和2004年的景观格局指数,对研究区17 a来的景观格局变化进行了分析。结果表明:该区域景观整体上处于破碎化过程,尤其是沙地在1987~1999年间迅速扩大,虽然在1999~2004年经过了一定的恢复,景观异质性有所下降,但仍然没有恢复到1987年的水平。     

Effect of proline-spirooxindole on viability and apoptosis of non-small-cell lung cancer A549 cells

AIM:To investigate the effect of proline-spirooxindole on the viability and apoptosis of human non-small-cell lung cancer A549 cells. METHODS:The effect of proline-spirooxindole on the viability of A549 cells was determined by CCK-8 assay. The apoptosis was analyzed by flow cytometry. The effects of proline-spirooxindole on the expression of PARP and p53 and the phosphorylation of mTOR were determined by Western blot. RESULTS:After A549 cells were treated with proline-spirooxindole (25, 50 and 100 mg/L), the cell viability was decreased (P<0.01) compared with DMSO control group. The apoptotic rate was increased compared with DMSO control group (P<0.01). The protein expression of p53 was up-regulated, the increased apoptotic protein cleaved PARP was observed, and the phosphorylation of mTOR was inhibited (P<0.01). CONCLUSION:Proline-spirooxindole inhibits the viability of A549 cells and induces apoptosis, which may be related to the phosphorylation of mTOR.     

基于landsat卫星影像的水库水体总磷质量浓度反演研究

以清河水库为研究区域,利用Landsat8卫星OLI数据及实地观测数据,建立了适于清河水库水体总磷质量浓度的最小二乘支持向量机(LS-SVM)遥感反演模型,并对清河水库总磷量进行了反演分析。结果表明,LS-SVM模型的平均相对误差为6.06%,相比于单波段线性回归反演模型及波段组合线性回归模型,平均误差分别降低了20.77%、12.53%,显著提高了清河水库水体总磷质量浓度反演的模型精度,达到遥感反演预测精度要求;利用LSSVM反演模型对清河水库总磷量反演显示,水体中总磷质量浓度主要集中在0.04~0.08 mg/L,水库水体总磷质量浓度总体偏高。     

外源性CNT对3 H-赖氨酸在哈白兔体内代谢动力学的影响

以哈白兔为试验动物,利用同位素示踪法研究外源环核苷酸(CNT)对3H-标记的赖氨酸(3H-Lysine)在动物体内的分布、转运与代谢影响,其示踪动力学可用二室模型来描述:^Y(t)=983.6281e-0.021935t+1773.9999e-0.083932t-983.6281e-0.432590t-0773.9999e-0.050399t+300.2820。研究结果证明,皮下注射95h后,对照组3H-Lysine主要分布于肾脏、心脏、肝脏、脾脏、肌肉中;cGMP组中主要分布分布于膀胱、肌肉、肺脏、肠中;cAMP组主要分布于肌肉、胃、肝脏、心脏、生殖器中;cGMP+cAMP组中主要分布于肌肉、膀胱、生殖器、脂肪。同时,心脏、肝脏、脾脏、肾脏、肌肉、脑、膀胱、肠器官中,皮下注射环核苷酸后均有显著(p<0.05)或极显著(p<0.01)的变化,说明外源性CNT对赖氨酸在哈白兔血液中的代谢及在组织和器官中分布均有明显影响。