大豆磷脂羟酰化的研究

对大豆磷脂羟酰化的研究，所得产品具有良好的乳化性、润湿性和乳化稳定性．结果表明，过氧化氢加入量对产品的质量和主要性质有显著影响，温度对碘值和过氧化值分别有一定的和显著影响，低压蒸汽的量对过氧化值有一定的影响，氢氧化钠加入量对乳化稳定性有一定的影响，并得出磷脂羟酰化的最佳工艺条件．     

不同激素配比对辣木组培苗生长的影响

以辣木嫩梢茎段为外植体,在25种不同激素配比的培养基上进行培养,探讨不同激素配比对辣木组培苗生长的影响,并在6种不同生根培养基上进行生根试验。研究结果表明:在MS+6-BA0.02～0.5mg.L-1+NAA0.02mg.L-1+蔗糖3%的培养基上辣木组培苗生长良好,增殖倍数达5.6～8.1。在1/2MS+NAA0.2mg.L-1+蔗糖2%的培养基上生根率最高达79%。用形态学上端朝上与朝下两种方式接种到生根培养基上均能在形态学下端生根。     

大豆疫霉慢生长菌株HN-1的生物学特性及传染规律

观察和测定了大豆疫霉(Phytophthora sojae)慢生长菌株HN-1及其单游动孢子后代的培养性状及致病力,并将其与正常生长的其他3个菌株进行对峙培养,研究其慢生长特性传染规律,测定受感染菌株的培养特性及致病力.结果表明,大豆疫霉慢生长菌株HN-1的培养特性与正常菌株相比生长显著缓慢,菌落形态有明显差异,卵孢子产生量低,对大豆幼苗的致病力显著较弱;该菌的慢生长特性可通过无性繁殖传递至子代,并可通过对峙培养传染至其他正常生长菌株,传染后产生的受染菌株表现慢生长特性且致病力减弱.     

模糊多维综合评判在两系杂交粳稻新品种选育中的应用

应用模糊多维综合评判方法,对2010年自育两系杂交粳稻新组合进行多因素综合评价分析,结果表明,9优418(D11)评价指标是0.7329,综合表现最优,其次是1586S/爪哇稻(D14),其评价指标为0.7166,1592S/新选1号(D15)评价指标是0.2152,综合表现最差,此结果与试验地实际表现吻合。     

猕猴血清B病毒抗体3种检测方法的比较

为了解猕猴B病毒3种检测方法的检测效果,用3种方法对猕猴血清B病毒抗体分别进行检测并比较。结果表明,幼年组HSV-1EIA法检测结果与BV ELISA法比较差异显著(P<0.05),HSV-1EIA法与BV EIA法符合率96.6%,HSV-1EIA法与BV ELISA法符合率95.3%,BV EIA法与BVELISA法符合率98.7%。表明3种抗体检测方法检测结果符合率较高,均可以做为初筛检测,但BVELISA法在幼年猕猴血清B病毒抗体检测上敏感性最好。     

Chemerin analog regulates energy metabolism in sheep

Accumulating data suggest a relationship between chemerin and energy metabolism. Our group previously described gene cloning, expression analysis and the regulatory mechanism of chemerin and its own receptor in mice and cattle. The objective of the present study was to investigate the physiological effect of chemerin on endocrine changes and energy metabolism in sheep using a biologically stable chemerin analog. The chemerin analog was intravenously administrated (100 or 500 µg/head) to sheep, and plasma insulin and metabolites (glucose, nonesterified fatty acids (NEFA), triglyceride, total cholesterol and high‐density lipoprotein (HDL) cholesterol) were analyzed. The chemerin analog dramatically increased the insulin levels, and glucose levels were decreased. NEFA levels were slightly decreased at 20 min but then increased gradually from 60 to 180 min after analog administration. In addition, injection of the chemerin analog immediately increased triglyceride and total cholesterol but not HDL levels. These results suggested that chemerin analog regulated insulin secretion related to glucose metabolism and the release of triglycerides in sheep in vivo. This study provides new information about endocrine and metabolic changes in response to chemerin in sheep.     

试论加强青海高原对外制种环境保护问题

加强对农作物制种环境的保护工作,抓好农田有害生物发生的预测预报和防治,特别是防止农作物检疫对象的发生蔓延是事关国家“三农”工作和粮食安全,保持农业可持续健康稳定发展的重大问题,也是当前农业相关部门面临的现实课题.     

Effect of Maternal Age on the Ratio of Cleavage and Mitochondrial DNA Copy Number in Early Developmental Stage Bovine Embryos

Age-associated deterioration in both the quality and quantity of mitochondria occurs in older women. The main aim of this study was to examine the effect of age on mitochondrial DNA copy number (mtDNA number) in early developmental stage bovine embryos as well as the dynamics of mtDNA number during early embryo development. Real-time PCR was used to determine mtDNA number. In vitro-produced embryos 48 h after insemination derived from Japanese black cows, ranging in age from 25 to 209 months were categorized based on their cleavage status. There was an overall negative relationship between the age of the cow and cleavage status, to the extent that the ratio of embryos cleaved over the 4-cell stage was greater in younger cows. The mtDNA number did not differ among the cleaved status of embryos. In the next experiment, oocytes collected from each donor cow were divided into 2 groups containing 10 oocytes each, in order to compare the mtDNA number of mature oocytes and early developmental stage embryos within individuals. Upon comparing the mtDNA number between oocytes at the M2 stage and early developmental stage 48 h post insemination, mtDNA number was found to decrease in most cows, but was found to increase in some cows. In conclusion, age affects the cleaving ability of oocytes, and very old cows (> 180 months) tend to have lower mtDNA numbers in their oocytes. The change in mtDNA number during early development varied among individual cows, although overall, it showed a tendency to decrease.