柿绒粉蚧种群空间分布及抽样技术探讨

根据系统调查资料,对柿绒粉蚧 (Eriococcus kaki kuwnan) 在柿树上的空间分布及抽样技术进行了初步探讨,其结果为:柿绒粉蚧种群密度在树冠不同部位的顺序为,垂直方向,下部树冠>中部树冠>上部树冠,下部树冠四个方向依次为西>南>东>北,内外两层以外层密度为高,柿绒粉蚧的空问分布为聚集型,聚集密度在不同部位有所差异,总的表现为低密度,高聚块,高密度,低聚块。在t=1,D=0.2,其理论抽样数为681个样方,但只以东西两侧为取样单位时,同样条件下,理论抽样数下降为475个样方。     

红外光谱法测定红松的木素含量

研究了红松红外光谱的相对吸光度与木素含量的关系。所取的吸收带有2920cm~(-1),1600cm~(-1),1510cm~(-1),1316cm~(-1),1160cm~(-1),895cm~(-1),实验结果表明,相对吸光度A_(1510/895)与红松中的木素含量有较好的相关性,基线以通过840cm~(-1)和1550cm~(-1)处的峰肩作水平线较好。用这种基线时,可用二条直线分别表示不同木素含量情况下A_(1510/895)与C_(本素)的关系。它们的回归方程分别为:当A_(1510/895)=1.6—3.0时,C_(本素)=2.597A_(1510/895)+20.38;当A_(1510/895)=0.7—1.6时,C_(本素)=1.481A_(1510/895)—0.695。用这种方法测定脱脂木粉及其脱木素过程中试样的木素含量,绝对误差分别不超过0.17％和1.58％。     

Research Status and Prospect of Larix spp.Wood Modification

Larix spp.wood is one of the major fast-growing tree species in China,which also has the most forest storage in the northeast of China.Several key properties modification methods and achievements of Larix spp.wood were summarized based on their characteristics.Technology problems of properties modification and new use prospect of modified Larix spp.wood were all discussed.     

寄生柑橘和杉木的柑橘半穿刺线虫种内群体变异

[目的]对寄生于柑橘与杉木2种寄主的柑橘半穿刺线虫种群的rDNA-ITS区(ITS1-5.8S-ITS2)及形态变异进行研究,分析种群间遗传多态性及其亲缘关系.[方法]对采自中国3个省份(福建、四川、贵州)柑橘产区的21个柑橘半穿刺线虫种群(柑橘种群)及福建省杉木林区的6个柑橘半穿刺线虫种群(杉木种群)的ITS区进行PCR扩增、克隆与测序,通过相关软件进行序列比对与系统发育分析;对3个柑橘种群及1个杉木种群进行形态特征测计分析.[结果]柑橘半穿刺线虫的柑橘种群与杉木种群相比,ITSI序列相似性为93.2%-99.3%,ITS2序列相似性为92.4%-100%;基于ITS序列构建的系统发育树,所有杉木种群与福建省部分柑橘种群处于同一进化主分枝.4个柑橘半穿刺线虫种群间部分测量值存在显著差异,补充描述了种群内二龄幼虫尾部形态变异类型.[结论]柑橘半穿刺线虫柑橘种群和杉木种群在rDNA-ITS序列相似性高,序列差异不能用于种群的区分;杉木种群与福建部分柑橘种群在进化上可能有相同的地理起源.种群间部分测量值的显著差异及二龄幼虫尾部变异应属于种群正常变异.     

胎羊肾细胞用于增殖H1N1亚型流感病毒的初步研究

为研究胎羊肾细胞用于H1N1亚型流感病毒增殖的可能性,采用胰酶消化法从胎羊肾组织中分离肾细胞进行传代培养,并采用间接免疫荧光法检测细胞表面的流感病毒受体,采用直接血凝法测定病毒培养物的血凝价。结果表明,该细胞表面有较丰富的流感病毒受体,且α2,6-Gal受体的含量明显高于犬肾细胞(MDCK);用该细胞培养H1N1亚型流感病毒,其病毒培养物的血凝价达到29～210,略高于MDCK细胞的毒价;该细胞在体外连续传10代,其病毒培养物的血凝价没有明显改变。以上结果初步表明胎羊肾细胞可用于H1N1亚型流感病毒的增殖。     

农业科研单位科研项目管理中存在的问题与对策

科研项目管理是科学研究的重要组成部分,对科学研究、科技发展以及自主创新能力的增强具有重要作用。分析农业科研单位科研项目管理中存在的问题,从加强科研项目全过程服务、打造高绩效的科研团队、完善科研评价机制、提高科研管理人员的综合素质4个方面提出应对策略,实现农业科研单位科研项目管理工作的可持续发展。     

西藏高原棚栽黄瓜花打顶的原因及防治方法

本文针对西藏高原温室、大棚造成黄瓜花打顶的原因与症状,从黄瓜适期定植、营养诊断,对症下药及科学管理,根据病情减轻植株负荷,喷施植物生长激素等方面总结出黄瓜花打顶的具体防治措施。     

High doses of FSH induce autophagy in bovine ovarian granulosa cells via the AKT/mTOR pathway

Follicle-stimulating hormone (FSH) plays a critical role in follicular growth and granulosa cell function; however, the mechanism by which the aggressive stimulation of FSH leads to poorer oocyte quality and embryo development potential is unclear. In this study, bovine ovarian granulosa cells (BGCs) were challenged with FSH doses (vehicle, 0.1, 1, 10 and 100 ng/ml) to investigate the effects of FSH on BGCs. The results indicated that the relative viability of BGCs was significantly increased in cells challenged with 1 ng/ml FSH, whereas the viability was significantly decreased with 100 ng/ml FSH treatment. The mRNA abundance of FSHR, CYP19, StAR and BAX was significantly upregulated with 1, 10 and 100 ng/ml of FSH, while the BCL-2 mRNA level was downregulated with higher concentrations of FSH (10 and 100 ng/ml). Furthermore, BGC autophagy was detected in cells treated with 10 and 100 ng/ml FSH by MDC staining, and the mRNA abundance of LC3, BECN1, BNIP3, ATG3 and ATG7 was upregulated with increasing FSH concentration. Meanwhile, the protein expression of LC3 was increased in cells treated with 10 and 100 ng/ml FSH. 1 and 10 ng/ml FSH significantly increased E2 production, whereas 10 and 100 ng/ml FSH significantly increased P4 production. FSH significantly inhibited the phosphorylation of AKT in cells treated with higher concentrations (1, 10 and 100 ng/ml), while activating mTOR phosphorylation at concentrations of 10 and 100 ng/ml of FSH. In summary, we can conclude that higher doses of FSH (10 and 100 ng/ml) induce BGC autophagy via the AKT/mTOR signalling pathway.     

森林健康经营理论应用研究——以塞罕坝机械林场为例

以塞罕坝林区为例,针对森林健康状况,在森林经营中引入森林健康经营理念,构建了完整森林健康经营技术措施。按照《森林健康经营技术研究》理论,分基础性指标、结构性指标、抗干扰性指标、生态服务价值评价等4类综合性指标对森林经营实践应用进行评析,并提出改进措施,促进森林健康经营理论应用。