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排序方式: 共有1164条查询结果,搜索用时 15 毫秒
121.
Nakamura RK Rishniw M King MK Sammarco CD 《Journal of Feline Medicine and Surgery》2011,13(4):266-271
The objective of this prospective study was to determine the prevalence of echocardiographic evidence of heart disease in apparently healthy cats with heart murmurs. Thirty-two privately owned domestic cats were examined. All cats were considered healthy on the basis of history and physical examination, except for the finding of a heart murmur on auscultation. Cats on any medications (besides regular flea, tick and heartworm preventative) or that were pregnant or lactating were excluded from this study. The prevalence of echocardiographic evidence of heart disease in this population of cats was 53%. Therefore, identification of a heart murmur on routine physical examination in apparently healthy cats warrants further investigation. 相似文献
122.
Shinsuke Yamanaka Ikuo Nakamura Hirokazu Nakai Yo-Ichiro Sato 《Genetic Resources and Crop Evolution》2003,50(5):529-538
The direct ancestor of rice (Oryza sativa L.) is believed to be AA genome wild relatives of rice in Asia. However, the AA genome wild relatives involve both annual and perennial forms. The distribution of the retrotransposon p-SINE1-r2, a short interspersed nuclear element (SINE) at the waxy locus was analyzed in diverse accessions of the AA genome wild relatives of rice (O. rufipogon sensu lato). Most annual wild rice accessions had this retrotransposon, while most perennial types lacked this element, contradicting results to the previous studies. Results presented here suggest that O. sativa has dual origin that lead to indica-japonica differentiation. Results suggest the indica line of rice varieties evolved from the annual genepool of AA genome and the japonica varieties from the perennial genepool of AA genome wild rice. 相似文献
123.
Nakamura Y Suganuma E Matsuo T Okamoto S Sato K Ohtsuki K 《Journal of agricultural and food chemistry》1999,47(2):544-549
Fushimi sweet pepper, "Fushimi-togarashi", is one of the "Kyo-yasai", traditional vegetables, in Kyoto, Japan. The chloroform fraction of Fushimi sweet pepper showed bioantimutagenicity on UV induced mutation in Escherichia coli B/r WP2. The bioantimutagen was purified with silica gel chromatography and identified as 2, 4-nonadienal (ID(50) = 20 microg/plate) on the basis of GC retention time and EI-MS spectrum of authentic 2,4-nonadienal. The sweet pepper also contained a known bioantimutagen, benzaldehyde (ID(50) = 2 mg/plate). Additive bioantimutagenicity was also observed by 2, 4-nonadienal with benzaldehyde. 2,4-Nonadienal did not show bioantimutagenicity in an UV excision repair deficient strain, E. coli B/r WP2s uvrA(-)(). Furthermore no delay of the first cell division after UV irradiation was observed in E. coli B/r WP2. These results indicate that the bioantimutagenic activity of 2, 4-nonadienal on UV mutagenesis might depend on the excision repair system in E. coli B/r WP2. 相似文献
124.
Shoji T Mutsuga M Nakamura T Kanda T Akiyama H Goda Y 《Journal of agricultural and food chemistry》2003,51(13):3806-3813
Procyanidin fractions from apple were separated according to the degree of polymerization using normal phase chromatography. Evaluation of physiological functionalities of procyanidins requires individual structural determination. However, it is difficult to elucidate the structure of procyanidins, in particular those with (+)-epicatechin (1) or (-)-catechin (2) units, and determine whether the interflavanoid bonds are 4beta-->8 or 4beta-->6 without cleavage and acetylation. Structural determination used LC-MS and low-temperature NMR. Nine procyanidins were separated by preparative HPLC consisting of three well-known procyanidins [procyanidin B1 (3), procyanidin B2 (4), and procyanidin C1 (5)] and six new procyanidins [epicatechin-(4beta-->8)-epicatechin-(4beta-->8)-catechin (6); epicatechin-(4beta-->6)-epicatechin-(4beta-->8)-catechin (7); epicatechin-(4beta-->6)-epicatechin-(4beta-->8)-epicatechin (8); epicatechin-(4beta-->8)-epicatechin-(4beta-->6)-catechin (9); epicatechin-(4beta-->8)-epicatechin-(4beta-->6)-epicatechin (10); and epicatechin-(4beta-->8)-epicatechin-(4beta-->8)-epicatechin-(4beta-->8)-epicatechin (11)]. Compounds 6-11 were detected for the first time as apple constituents. 相似文献
125.
H Kikuchi A Nakamura K Tsuji 《Journal of the Association of Official Analytical Chemists》1988,71(5):1057-1062
A sensitive method is described to determine trace quantities of ethylene oxide (EO) in EO-sterilized intraocular lenses (IOLs). An IOL is dipped in ethanol containing 0.25 ppm propylene oxide (PO) in a 4 mL vial, 2 drops of freshly distilled hydrobromic acid is added through a septum, and the mixture is warmed at 50 degrees C for 24 h. It is then neutralized by vigorous shaking with sodium bicarbonate, dehydrated with anhydrous sodium sulfate, and filtered. The filtrate is injected into a gas chromatograph with electron-capture detection, and the peak height ratio of ethylene bromohydrin/propylene bromohydrin is measured. EO residue is calculated from the calibration curve obtained through a similar procedure with the standard EO/PO solutions. The limit of determination is 0.04 microgram/lens (ca 2.0 ppm). When EO residue levels were determined for IOLs sampled at 3 different aeration periods after sterilization, we found that 9 days of aeration was necessary to meet the U.S. Food and Drug Administration proposed limit for EO residue in IOLs. 相似文献
126.
R. Ishikawa S. Yamanaka Y. Fukuta S. Chitrakon C. Bounphanousay K. Kanyavong L.-H. Tang I. Nakamura T. Sato Y.-I. Sato 《Genetic Resources and Crop Evolution》2006,53(2):245-252
The purpose of this study was to assess the extent of genetic erosion of traditional upland germplasm in northern Thailand
as a result of gene-flow from distinct strains carrying different genotypes. Even modern variety specific markers have not
been developed, there is a comparative population in Laos. Thus, both populations were compared with various characters to
evaluate gene-flow from modern variety to landraces. Glutinous and glabrous strains are predominated in Laos. However, such
strains were drastically decreased in north–east Thailand. Gene diversity is higher in Thailand, compared to Laos at seven
isozyme loci. This was a result of the higher frequencies of Indica strains and heterozygotes in Thailand. Plastid type was
also determined by using an INDEL marker. Nearly half of Indica strains carried the Japonica plastid. Heterozygotes also tended
to carry Japonica cytoplasm. Such nuclear–cytoplasm substituted strains and heterozygotes were probably generated by natural
hybridization. Japonica strains tended to be a maternal donor rather than Indica ones. Or Indica strains would easily release
pollens, which grow outside of upland fields. 相似文献
127.
Hu F Furihata K Ito-Ishida M Kaminogawa S Tanokura M 《Journal of agricultural and food chemistry》2004,52(16):4969-4974
In this study were successfully observed the one- (1H, 13C) and two-dimensional (1H-13C, 1H-15N, 1H-31P) NMR spectra of milk directly without any pretreatment. The signals of each NMR spectrum were assigned, and their existing states were also analyzed. Lactose existed in a free state in milk. The signals due to the butyric acid chain can be assigned among the other fatty acid chains. Monounsaturated fatty acid (oleic acid chains) and polyunsaturated fatty acid chains (linoleic and linolenic acid) were assigned by their characteristic signals. The signals from citrate, N-acetylcarbohydrates, and lecithin could be observed directly in the 1H-13C HSQC NMR spectra; the assignment of their signals was made through the 1H-13C, 1H-15N, and 1H-31P HMBC spectra of extracted milk. Signals from creatine and N-acetylcarbohydrates were detected for the first time. 相似文献
128.
Pathological changes of tracheal mucosa in chickens infected with lentogenic Newcastle disease virus 总被引:1,自引:0,他引:1
Forty-day-old specific-pathogen-free chickens were exposed by aerosol to lentogenic Newcastle disease virus (NDV) and observed for 24 days for pathological changes in the tracheal mucociliary system. Specific fluorescence of NDV antigen was observed through day 5 postexposure (PE) in the cytoplasm of the tracheal epithelium and desquamated epithelium in the lumen. On day 1 PE, scanning electron microscopy revealed hypertrophy of goblet cells and small patches of the deciliated epithelium scattered mainly around the openings of mucous glands. The deciliated area of tracheal surface increased through day 4 PE. Light microscopy showed small vacuoles containing lymphocytes and heterophils in the epithelial layer. Immature epithelium proliferated in some areas. On days 5 and 6 PE, ciliated areas of the trachea tended to increase as a result of regeneration of the epithelium, still leaving many nonciliated patches of various sizes. On and after day 8 PE, there remained plaques with nonciliated flat epithelium, but most areas were covered with well-ciliated epithelium. Non-ciliated plaques were observed until day 24 PE, but they gradually decreased in size. These plaques were covered by a single layer of flat epithelium and were formed upon lymph follicles in subepithelial tissue. 相似文献
129.
梨果实发育中Ca2+在果肉细胞的定位及变化研究* 总被引:17,自引:3,他引:14
用焦锑酸钾沉淀的细胞化学方法, 研究了 幸水 梨果实发育中果肉细胞的焦锑酸Ca2+ 定位变化及其与细胞超微结构的关系。结果表明: ( 1) 在未受精之前, 果肉细胞内未检测到Ca2+ 沉淀颗粒, 细胞核内的染色质少且染色淡, 细胞质的细胞器数量也少; ( 2) 受精后果肉细胞呈现大量的Ca2+沉淀颗粒, 主要分布在细胞核、细胞质、质体以及叶绿体外膜上, 含Ca2+沉淀颗粒的质体非常膨大, 导管和初期发育的石细胞内也密集分布Ca2+ 沉淀颗粒; ( 3) 受精1 周后果肉细胞的Ca2+移向细胞之间的连接处; ( 4) 生理落果的细胞和导管中Ca2+没有或极少, 但有的细胞内沿液泡膜有Ca2+分布; ( 5) 受精3~ 4 周后, 果肉细胞中很难检测到Ca2+沉淀颗粒, 此状态一直持续到果实采收, 但果实腐烂前Ca2+沉淀颗粒沿果肉细胞
壁两侧出现。就Ca2+ 在果实发育中的作用及与细胞超微结构的关系等进行了讨论。 相似文献
130.
Escherichia coli numbers and histopathological changes were studied in the respiratory tract of line 151 chickens intranasally inoculated with infectious bronchitis virus (IBV) and/or virulent E. coli; this line is highly susceptible to IBV. Chickens inoculated with IBV alone showed increased numbers of E. coli in the trachea and had tracheitis, airsacculitis, and bronchiolitis. One of 17 chickens inoculated with IBV alone died with fibrinopurulent serositis. Chickens inoculated with IBV and E. coli had more severe and persistent respiratory lesions than those inoculated with IBV alone. E. coli was isolated from tracheas of chickens inoculated with IBV and E. coli more frequently than from chickens inoculated with IBV alone. In this group, 14 of 27 chickens died with tracheal plugs or with fibrinopurulent serositis. There was neither increased numbers of E. coli nor significant lesions in the respiratory tract of the group inoculated with E. coli alone. These results suggest that IBV may facilitate E. coli invasion into the lower respiratory tract of the chicken. 相似文献