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21.
Kitadai N Obi T Yamashita S Murase T Takase K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(3):395-397
Susceptibility to 13 antimicrobial agents was examined for 138 Escherichia coli isolates obtained from 192 fecal samples of wild cranes that migrated for wintering to the Izumi plain, Kagoshima prefecture in Japan. The numbers of isolates that were resistant to the antimicrobials used in this study are as follows: oxytetracycline (OTC), 22 isolates; minocycline, 7 isolates; ampicillin (ABPC), 4 isolates; nalidixic acid, 4 isolates; enrofloxacin, 2 isolates; kanamycin, one isolate. Multidrug resistant isolates exhibiting 2-4 drug resistances were obtained. All of the OTC-resistant isolates carried either the tet (A) or tet(B) gene. The bla(TEM) gene was found in all of the ABPC-resistant isolates. 相似文献
22.
Hideki OHSAKI Atsuko TANAKA Shogo HOASHI Shinji SASAZAKI Kenji OYAMA Masaaki TANIGUCHI Fumio MUKAI Hideyuki MANNEN 《Animal Science Journal》2009,80(3):225-232
Fatty acid composition of beef adipose tissue is one of important traits because high proportions of monounsaturated fatty acid are related to favorable beef flavor and tenderness. In this study, we investigated effects of genetic factors such as stearoyl-CoA desaturase (SCD) and sterol regulatory element binding protein (SREBP) on beef carcass traits including fatty acid composition using two cattle populations. Sire effect was significantly related to almost all traits except BMS, suggesting that the trait examined in this study is highly controlled by genetic factors. The effect of SCD genotype on fatty acid composition was detected remarkably in both cattle groups, especially on stearic and oleic acids. This result was consistent with our previous studies and suggests that SCD is associated with fatty acid composition. Unlike SCD genotyping, the effect of SREBP genotype was not identified in this study. Our results suggested that SCD genotype would contribute to improving beef quality in field populations. Further studies about the relationship among these factors will bring an insight into the molecular mechanism of fatty acid metabolism in cattle. 相似文献
23.
Shogo HIGAKI Hongyu DARHAN Chie SUZUKI Tomoko SUDA Reina SAKURAI Koji YOSHIOKA 《The Journal of reproduction and development》2021,67(1):67
We aimed to determine the effectiveness of estrus detection based on continuous measurements of the ventral tail base surface temperature (ST) with supervised machine learning in cattle. ST data were obtained through 51 estrus cycles on 11 female cattle (six Holsteins and five Japanese Blacks) using the tail-attached sensor. Three estrus detection models were constructed with the training data (n = 17) using machine learning techniques (random forest, artificial neural network, and support vector machine) based on 13 features extracted from sensing data (indicative of estrus-associated ST changes). Estrus detection abilities of the three models on test data (n = 34) were not statistically different among models in terms of sensitivity and precision (range 50.0% to 58.8% and 60.6% to 73.1%, respectively). The relatively poor performance of the models might indicate the difficulty of separating estrus-associated ST changes from estrus-independent fluctuations in ST. 相似文献
24.
The object of this study was to compare the mass concentration of airborne dust during circular sawing of five wood-based
materials: solid sugi (Cryptomeria japonica) lumber, tropical hardwood plywood, softwood plywood, particleboard, and medium-density fiberboard. Specimens were sawn at
a constant feed per tooth (0.05 mm) using two saw speeds. The mass concentration of airborne dust of diameter 7.07 μm or less
(respirable dust) was measured with a light-scattering dust monitor. The mass concentration showed a log-normal distribution,
and the geometric means of mass concentration at saw speeds of 2000 and 3000 rpm were 2.33 and 2.89 mg/m3 for tropical hardwood plywood, 1.13 and 2.84 mg/m3 for particleboard, 0.91 and 2.28 mg/m3 for medium-density fiberboard, 1.09 and 1.38 mg/m3 for softwood plywood, and 0.32 and 0.66 mg/m3 for sugi lumber. The mass concentration for all five wood-based materials increased with the revolution speed of the circular
saw. 相似文献
25.
Soichi?TanakaEmail author Yuko?Fujiwara Yoshihisa?Fujii Shogo?Okumura Hiroyoshi?Togo Naoya?Kukutsu Tadao?Nagatsuma 《Journal of Wood Science》2011,57(3):189-194
The attenuation coefficients of 100-GHz millimeter waves polarized linearly were measured for cross-cut, quarter-sawn, and
flat-sawn boards of hinoki (Chamaecyparis obtusa) that were 0.2–2.0 cm thick. This was done to examine the applicability of free-wave propagation theory for applying electromagnetic
waves to wood. It was found that the transmittance of a millimeter wave through the specimen boards was lower when the fiber
direction of a board was parallel to the direction of the electric field of the incident wave than when the fiber direction
was perpendicular to the electric field, and there was little difference in the transmittance between the tangential and radial
directions for the former case. These findings can be quantitatively explained by using propagation theory and the dielectric
properties of wood. 相似文献
26.
Yamasaki S Hara K Izumiya H Watanabe H Misawa N Okamoto K Takase K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(8):813-818
One hundred twenty Salmonella Enteritidis isolates collected from 1992 to 2005 in Nagasaki prefecture (65 isolates from 40 outbreak cases, 44 from sporadic diarrhea patients, and 11 from chicken-related products) were investigated by their antibiotic susceptibility profiles, phage typing, and pulsed-field gel electrophoresis (PFGE) typing. Out of them, 18 were identified as lysine decarboxylase (LDC)-negative isolates, and 15 showed resistance toward streptomycin. Based on the PFGE typing, the isolates were classified into five clusters by UPGMA clustering method. Three LDC-negative isolates belonged to cluster A and were of phage type (PT) 4 and isolated between 2000 and 2004. Other 15 LDC-negative isolates belonged to cluster E. They were PT1, reacted but did not conform (RDNC), or untypable and were isolated between 2001 and 2004. LDC-negative isolates of the cluster A differed from LDC-negative isolates of the cluster E in antibiotic susceptibility profiles, phage typing, and PFGE typing. LDC-negative isolates of the cluster E were isolated after 2001 in Nagasaki prefecture. 相似文献
27.
Yamada K Taniura T Tanabe S Yamaguchi M Azemoto S Wisner ER 《Journal of veterinary medical education》2007,34(2):143-150
The purpose of the study reported here was to establish a method of teaching veterinary anatomy, including radiologic anatomy, for clinical practice using computer-aided diagnosis (CAD). Two clinically healthy dogs and three cats were scanned using multi-detector row computed tomography (MDCT). Images were made by means of imaging processing software. At the workstation, by observing the transverse, dorsal-plane, or sagittal sections and three-dimensional (3D) images simultaneously, it is much easier to understand the 3D anatomical structure. With this educational support system, anatomical figures can be explained using living animals instead of specimens. In addition, clinical representative examples can be used to show anatomical disorders to students. Veterinary students (N = 62) who filled out a questionnaire evaluating how the method aided their understanding of both experimental study and clinical examples gave it a score of 88.2 +/- 20.6 (Mean +/- SD) on a visual analog scale. This system can enhance veterinary students' understanding and interest in anatomy and can enable us to offer them a quality veterinary medical education. We concluded that CAD is a useful new option not only for clinical service but also for veterinary education. 相似文献
28.
Yoshihiro KOBAYASHI Tetsushi HIRANO Takuya OMOTEHARA Rie HASHIMOTO Yuria UMEMURA Hideto YUASA Natsumi MASUDA Naoto KUBOTA Kiichi MINAMI Shogo YANAI Mitsuko ISHIHARA-SUGANO Youhei MANTANI Toshifumi YOKOYAMA Hiroshi KITAGAWA Nobuhiko HOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1355-1361
29.
Yoshiyuki Yanase Masahiro Miura Yoshihisa Fujii Shogo Okumura Tsuyoshi Yoshimura 《Journal of Wood Science》2013,59(3):243-248
A gas detection apparatus equipped with a semiconductor gas sensor was employed for qualitative and quantitative measurement of hydrogen and methane emitted by termites. A gas sample of 2.5 ml was injected into the semiconductor gas sensor through the gas detection apparatus, and the maximum voltage of the sensor was converted into gas concentration. The gas samples were collected from three distinct experiments: (1) five combinations of workers and soldiers of Coptotermes formosanus with and without a wood specimen; (2) C. formosanus under six temperature conditions; and (3) four different termite species, C. formosanus, Reticulitermes speratus, Incisitermes minor, and Zootermopsis nevadensis. The hydrogen and methane concentrations increased with an increase in the number of termites. Concentrations were higher in samples with a wood specimen than without it. Both hydrogen and methane concentrations were the highest for the samples at 35 °C and were lowest at 15 and 5 °C. The concentrations were very low at 45 °C because all the termites had died in a few hours. The concentrations of hydrogen and methane were highest for Z. nevadensis, the dampwood termite, among the four species, and no methane was detected for I. minor, the drywood termite, at 28 °C and 75 % RH. 相似文献
30.
Mami OIKAWA Shogo MATOBA Kimiko INOUE Satoshi KAMIMURA Michiko HIROSE Narumi OGONUKI Hirosuke SHIURA Michihiko SUGIMOTO Kuniya ABE Fumitoshi ISHINO Atsuo OGURA 《The Journal of reproduction and development》2013,59(3):231-237
In mice, one of the major epigenetic errors associated with somatic cell nuclear
transfer (SCNT) is ectopic expression of Xist during the preimplantation
period in both sexes. We found that this aberrant Xist expression could
be impeded by deletion of Xist from the putative active X chromosome in
donor cells. In male clones, it was also found that prior injection of
Xist-specific siRNA could significantly improve the postimplantation
development of cloned embryos as a result of a significant repression of
Xist at the morula stage. In this study, we examined whether the same
knockdown strategy could work as well in female SCNT-derived embryos. Embryos were
reconstructed with cumulus cell nuclei and injected with Xist-specific
siRNA at 6–7 h after oocyte activation. RNA FISH analysis revealed that siRNA treatment
successfully repressed Xist RNA at the morula stage, as shown by the
significant decrease in the number of cloud-type Xist signals in the
blastomere nuclei. However, blastomeres with different sizes (from “pinpoint” to “cloud”)
and numbers of Xist RNA signals remained within single embryos. After
implantation, the dysregulated Xist expression was normalized
autonomously, as in male clones, to a state of monoallelic expression in both embryonic
and extraembryonic tissues. However, at term there was no significant improvement in the
survival of the siRNA-injected cloned embryos. Thus, siRNA injection was largely effective
in repressing the Xist overexpression in female cloned embryos but failed
to rescue them, probably because of an inability to mimic consistent monoallelic
Xist expression in these embryos. This could only be achieved in female
embryos by applying a gene knockout strategy rather than an siRNA approach. 相似文献