全文获取类型
收费全文 | 79篇 |
免费 | 4篇 |
国内免费 | 1篇 |
专业分类
林业 | 1篇 |
7篇 | |
综合类 | 6篇 |
水产渔业 | 9篇 |
畜牧兽医 | 46篇 |
植物保护 | 15篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 9篇 |
2021年 | 8篇 |
2020年 | 1篇 |
2017年 | 3篇 |
2016年 | 1篇 |
2015年 | 7篇 |
2013年 | 3篇 |
2012年 | 3篇 |
2011年 | 1篇 |
2009年 | 2篇 |
2008年 | 3篇 |
2007年 | 3篇 |
2006年 | 7篇 |
2005年 | 4篇 |
2004年 | 5篇 |
2003年 | 8篇 |
2002年 | 4篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1994年 | 3篇 |
1992年 | 1篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1961年 | 1篇 |
排序方式: 共有84条查询结果,搜索用时 15 毫秒
81.
Antigenic relationships of selected strains of infectious pancreatic necrosis virus and European eel virus 总被引:1,自引:0,他引:1
Abstract. An antigenic comparison of ten strains of infectious pancreatic necrosis virus (IPNV) and a single strain of European eel virus (EEV) revealed the presence of three distinct groups separable by cross-neutralization. The 1/ r value calculated from the formula r=√r1 × r2 (Archetti & Horsfall 1950) was used to divide the virus strains into: group I, which included all six U.S.A. strains: Buhl, Idaho; Reno, Nevada; ATCC VR 299; Powder Mill, New Hampshire; West Buxton, Maine; and Cascade Locks, Oregon: group II, the European strains: ďHonnincthun, France; Sp Denmark; and Bonnamy, France: group III, Ab Denmark and EEV. The group II European strains of IPNV were more closely related to the group I U.S.A. strains than to those viruses in group III. European eel virus (EEV) was antigenically most closely related to the Ab strain of IPNV. 相似文献
82.
Three-dimensional (3D) photonic crystals containing artificial point defects have been fabricated to emit light at optical communications wavelengths. They were constructed by stacking 0.7-micrometer-period gallium arsenide striped layers, resulting in a 3D "woodpile" photonic crystal. Indium-gallium arsenide-phosphide quantum-well layers emitting at a wavelength of 1.55 micrometers were incorporated in the center of the crystal. Samples having up to nine stacked layers were constructed, and artificial point-defect cavities of different sizes were formed in the light-emitting layer. Light emission was suppressed in the photonic crystal regions, whereas cavity modes were successfully observed at the point defects and were size dependent. 相似文献
83.
Saki SAKUMA Mariko OKAMOTO Nao MATSUSHITA Masami UKAWA Takumi TOMONO Keiko KAWAMOTO Teruo IKEDA Shinji SAKUMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(4):484
Poly(N-vinylacetamide-co-acrylic acid) coupled with d-octaarginine (VP-R8) promotes the cellular uptake of peptides/proteins in vitro; however, details of the transfection efficacy of VP-R8, such as the cell types possessing high gene transfer, are not known. Herein, we compared the ability of VP-R8 to induce the cellular uptake of plasmid DNA in mouse and human cell lines from different tissues and organs. A green fluorescent protein (GFP)-expression plasmid was used as model genetic material, and fluorescence as an indicator of uptake and plasmid-derived protein expression. Three mouse and three human cell lines were incubated with a mixture of plasmid and VP-R8, and fluorescence analysis were performed two days after transfection. To confirm stable transgene expression, we performed drug selection three days after transfection. A commercially available polymer-based DNA transfection reagent (PTR) was used as the transfection control and standard for comparing transgene expression efficiency. In the case of transient transgene expression, slight-to-moderate GFP expression was observed in all cell lines transfected with plasmid via VP-R8; however, transfection efficiency was lower than using the PTR for gene delivery. In the case of stable transgene expression, VP-R8 promoted drug-resistance acquisition more efficiently than the PTR did. Cells that developed drug resistance after VP-R8-mediated gene transfection expressed GFP more efficiently than cells that developed drug resistance after transfection with the PTR. Thus, VP-R8 shows potential as an in vitro or ex vivo nonviral transfection tool for generating cell lines with stable transgene expression. 相似文献
84.
Mariko OKAMOTO Hirotaka FURUYA Ikuko SUGIMOTO Daisuke TAKAMATSU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(11):1453
American foulbrood (AFB) is a honeybee disease caused by Paenibacillus larvae, and tylosin is used as the prophylactic in Japan. Honey contains macrolide-resistant bacteria that are a potential source of genes that may confer tylosin resistance to P. larvae. To investigate the potential risk of such genes in Japanese honey, we developed real-time PCR assays for the detection of important macrolide resistance genes, ermC and ermB, and analyzed 116 Japanese honey samples with known contamination status of P. larvae. Consequently, 91.38% of samples contained ermC and/or ermB, and 71.55% of samples contained both ermC and P. larvae, suggesting the possible emergence of tylosin-resistant P. larvae in Japan. Therefore, judicious use of the prophylactic is essential in maintaining its effectiveness. 相似文献