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排序方式: 共有167条查询结果,搜索用时 15 毫秒
71.
Yuri ABE Tomokazu TAMURA Shiho TORII Shiho WAKAMORI Makoto NAGAI Kazuya MITSUHASHI Junki MINE Yuri FUJIMOTO Naofumi NAGASHIMA Fumi YOSHINO Yukihiko SUGITA Takushi NOMURA Masatoshi OKAMATSU Hiroshi KIDA Yoshihiro SAKODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):61-70
In our previous study, we genetically analyzed bovine viral diarrhea viruses (BVDVs)
isolated from 2000 to 2006 in Japan and reported that subgenotype 1b viruses were
predominant. In the present study, 766 BVDVs isolated from 2006 to 2014 in Hokkaido,
Japan, were genetically analyzed to understand recent epidemics. Phylogenetic analysis
based on nucleotide sequences of the 5′-untranslated region of viral genome revealed that
766 isolates were classified as genotype 1 (BVDV-1; 544 isolates) and genotype 2 (BVDV-2;
222). BVDV-1 isolates were further divided into BVDV-1a (93), 1b (371) and 1c (80)
subgenotypes, and all BVDV-2 isolates were grouped into BVDV-2a subgenotype (222). Further
comparative analysis was performed with BVDV-1a, 1b and 2a viruses isolated from 2001 to
2014. Phylogenetic analysis based on nucleotide sequences of the viral glycoprotein E2
gene, a major target of neutralizing antibodies, revealed that BVDV-1a, 1b and 2a isolates
were further classified into several clusters. Cross-neutralization tests showed that
BVDV-1b isolates were antigenically different from BVDV-1a isolates, and almost BVDV-1a,
1b and 2a isolates were antigenically similar among each subgenotype and each E2 cluster.
Taken together, BVDV-1b viruses are still predominant, and BVDV-2a viruses have increased
recently in Hokkaido, Japan. Field isolates of BVDV-1a, 1b and 2a show genetic diversity
on the E2 gene with antigenic conservation among each subgenotype during the last 14
years. 相似文献
72.
Wei W Kakizawa S Jung HY Suzuki S Tanaka M Nishigawa H Miyata S Oshima K Ugaki M Hibi T Namba S 《Phytopathology》2004,94(7):683-686
ABSTRACT Antisera raised against phloem-limited phytoplasmas generally react only with the phytoplasma strain used to produce the antigen. There is a need for an antiserum that reacts with a variety of phytoplasmas. Here, we show that an antiserum raised against the SecA membrane protein of onion yellows phytoplasma, which belongs to the aster yellows 16S-group, detected eight phytoplasma strains from four distinct 16S-groups (aster yellows, western X, rice yellow dwarf, and elm yellows). In immunoblots, approximately 96-kDa SecA protein was detected in plants infected with each of the eight phytoplasmas. Immunohistochemical staining of thin sections prepared from infected plants was localized in phloem tissues. This antiserum should be useful in the detection and histopathological analysis of a wide range of phytoplasmas. 相似文献
73.
灌浆结实期高温对水稻籽粒蔗糖及降解酶活性的影响 总被引:8,自引:0,他引:8
选用水稻品种越光和笹锦为材料,在开花后设置自然温度和高温两种处理,研究了不同温度处理下籽粒蔗糖、果糖和葡萄糖含量的动态变化以及蔗糖合酶、液泡型转化酶和细胞壁结合型转化酶活性的差异。高温处理下,蔗糖合酶活性值大于转化酶活性值,且与淀粉积累速率呈显著正相关,表明蔗糖合酶在蔗糖的分解和淀粉的合成过程中起着重要作用;高温下两品种籽粒的蔗糖含量明显增加,但分解的葡萄糖和果糖含量并未相应增加,表明高温有利于籽粒中蔗糖的积累,而不利于籽粒中蔗糖的分解。高温下蔗糖合酶、液泡型转化酶和细胞壁结合型转化酶活性明显下降,表明蔗糖分解速率的下降与蔗糖合酶和转化酶活性下降有关。 相似文献
74.
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76.
KENSUKE NAKAMURA NOBORU SASAKI MUNEYOSHI YOSHIKAWA HIROSHI OHTA SHIANG-JYI HWANG TAKAHIRO MIMURA MASAHIRO YAMASAKI MITSUYOSHI TAKIGUCHI 《Veterinary radiology & ultrasound》2009,50(1):104-108
Contrast-enhanced ultrasonography has an important role in the detection of tumors in humans. The second-generation contrast agent Sonazoid has the ability of real-time contrast imaging along with parenchymal imaging. The purposes of this study were to determine the effect and duration of Sonazoid on the changes in gray-scale enhancement of canine spleen and to establish an appropriate protocol for contrast-enhanced ultrasonography of canine spleen. Six healthy beagles were injected with an intravenous bolus of Sonazoid. In the spleen parenchyma, the enhancement was maintained up to 30 min after injection. Moreover, for 5–22 s after injection, gray-scale enhancement of splenic arteries afforded arterial imaging. Perfusion of the kidney may be investigated from 3.6 s to 3.5 min after injection of Sonazoid. These results suggest that Sonazoid is applicable to canine spleen parenchymal imaging and that the optimal time for the parenchymal imaging is 7–30 min after injection. The findings of this quantitative study should prove useful in the evaluation of diffuse or focal splenic and renal diseases in dogs. 相似文献
77.
Naoki SASAKI Nao TSUZUKI Michiaki YAMADA Takuto MINAMI Haruo YAMADA 《Journal of Equine Science》2009,20(1):7-10
This study aimed to establish a standard dose and sample collection time for
13C phenylalanine and 13C-Dipeptide breath test in horses. To
evaluate dose-dependent effects, healthy horses received 2.5 mg/kg, 5 mg/kg, and 10 mg/kg
13C phenylalanine dissolved in 1 ml/kg distilled water and 1.25 mg/kg, 2.5
mg/kg, and 5 mg/kg 13C dipeptide dissolved in 2 ml/ kg distilled water. Tmax
was observed during the sample collection time. For 13C phenylalanine, the
standard deviation of Cmax at 5 mg/kg was lower than that of 10 mg/kg. For 13C
dipeptide, the standard deviation of Tmax was the lowest at 5 mg/kg. This study revealed
that an optimal dose for breath tests with 13C phenylalanine and 13C
dipeptide may be 5 mg/kg in horses. 相似文献
78.
Kensuke HIROSE Mariko NAKAMURA Tatsuya TAKIZAWA Kazuo FUKAWA Tetsuya ITO Masayuki UEDA Takashi SASAKI Kazuaki TANAKA 《Animal Science Journal》2009,80(6):624-630
An insertion/deletion variant of a thymine base (T5 and T6) in exon 2 of porcine beta 3‐adrenergic receptor (ADRB3) gene has been described. In the current study, we made an association study between the ADRB3 polymorphisms and production traits in 735 Duroc pigs. The allele frequencies for the T5 and T6 alleles in our study population were 0.433 and 0.567, respectively. Any associations between ADRB3 genotype and average daily weight gain during test period, or backfat thickness and intramuscular fat content were not detected in either sex. However the size of the loin eye muscle area (EMA) was significantly associated with ADRB3 genotypes in gilts. T6‐homozygous gilts had a higher mean of EMA (40.6 ± 0.6 cm2) than T5‐homozygous (38.1 ± 0.4 cm2, P = 0.002) and heterozygous (38.8 ± 0.3 cm2, P = 0.034) gilts. This association was not detected in males. In addition, a multiple traits animal model best linear unbiased predictor (BLUP) analysis revealed that the T6‐homozygous genotype had positive effects on breeding value of EMA. Accordingly, we suggest that ADRB3 polymorphism has the potential to be an important genetic marker for prediction of EMA in Duroc pigs. 相似文献
79.
Ozaki S Oki N Suzuki S Kitamura S 《Journal of agricultural and food chemistry》2010,58(22):11593-11599
An arabinogalactan-protein (WSSP-AGP) was isolated from the tuberous cortex of the white-skinned sweet potato (WSSP; Ipomoea batatas L.). It consists of 95% (w/w) carbohydrate and 5% (w/w) protein with high contents of hydroxyproline, alanine, and serine. Its sugar composition is α-L-Rha:α-L-Ara:β-D-Gal:β-D-GlcA in a molar ratio of 1.0:4.1:7.6:1.3. Its weight-average molecular weight was estimated to be 126,800 g/mol by high-performance size exclusion chromatography coupled with multiangle laser light scattering. Structural analysis indicated that WSSP-AGP is a (1→3)-β-D-galactan highly branched at O-6 with (1→6)-β-D-galactan, in which the branched chains are substituted at the O-3 position with α-L-Araf-(1→ and α-L-Araf-(1→5)-α-L-Araf-(1→ and at the O-6 position typically with α-L-Rhap-(1→4)-β-D-GlcAp-(1→ as terminating groups. Continuous administration of WSSP-AGP to KKAy mice significantly lowered fasting plasma glucose levels. This indicates that WSSP-AGP plays an important role in the hypoglycemic effects of WSSP. 相似文献
80.
Shiho USUI Hidemi YASUDA Yuzo KOKETSU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1063-1069
The objectives of this study were to survey clinics’ guidance about recommended
fasting duration (FD) prior to lipoprotein analysis, and to characterize lipoprotein
cholesterol and triglyceride concentrations in obese and overweight dogs categorized on
the basis of the 5-point body condition score (BCS) scale. A dataset was created from
lipoprotein analysis medical records of 1,538 dogs from 75 breeds in 354 clinics from 2012
to 2013. A phone survey was conducted to obtain the clinics’ FD. Two-level linear
mixed-effects models were applied to the data. Over 50% of the clinics said they
recommended fasting for 12 hr or more. Dogs in clinics with FD 12 hr or more had lower
chylomicron triglyceride concentrations than those in clinics with FD less than 8 hr
(P=0.05). Mean (± SEM) BCS at sampling was 3.7 ± 0.02. Obese and
overweight dogs had higher very low density lipoprotein (VLDL) and high density
lipoprotein (HDL) cholesterol and triglyceride concentrations than ideal dogs
(P<0.05), but no such difference was found for low density
lipoprotein cholesterol and triglyceride concentrations (P≥0.07). Across
all BCS, as dog age rose from 0 to 8 years old, HDL cholesterol concentrations decreased
by 13.5 mg/dl, whereas VLDL triglyceride concentrations increased by 81.7
mg/dl (P<0.05). In conclusion, FD of 8 hr or less
may affect lipoprotein lipid concentrations. Obese and overweight dogs were characterized
as having high VLDL and HDL cholesterol and triglyceride concentrations. 相似文献