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141.
Joseph Hill Jessica Lawrence Corey Saba Michelle Turek Brittany Feldhaeusser Sheryl Coutermarsh-Ott Jamie Barber Jo Smith Robert Gogal Jr 《Research in veterinary science》2014
Feline injection site sarcoma (ISS) is a locally invasive tumor, in which surgical treatment is frequently combined with radiation or chemotherapy to improve tumor control. The focus of this study was to evaluate the cytotoxic effects of doxorubicin or etoposide on a feline injection site sarcoma cell line (JB) and to assess the impact of combining these drugs on cell death and cell cycle. Both single agent and combination drug administration increased cell death and significantly reduced the number of viable cells. Cells in G0/G1 were significantly reduced while the G2/M fraction was significantly increased following treatment. Collectively, combining doxorubicin and etoposide at the lower EC yielded comparable results to the EC50 of either drug alone in degree of cytotoxicity, level of apoptosis, and % of cells in G2/M. The results of this study indicate that doxorubicin and etoposide alone and in combination differentially alter ISS cell viability and cycle. 相似文献
142.
O'Donoghue EM Somerfield SD Shaw M Bendall M Hedderly D Eason J Sims I 《Journal of agricultural and food chemistry》2004,52(17):5383-5390
The storage, soluble, and structural carbohydrates of two onion cultivars, the hard, pungent Pukekohe Longkeeper (PLK) and the softer, milder Houston Grano, were analyzed to determine differences that might be related to their response to sulfur nutrition received during growth as well as their postharvest attributes and end-use suitability. PLK tissue contained 1.37 times more dry matter than Grano and was composed of more fructan and sucrose and less glucose and fructose than Grano [corrected] There were also differences in neutral sugar content, especially galactose, and the amount, size, and content of pectin fractions soluble in chelator and weak alkali. These two onion cultivars differed in their capacity to take up sulfur, but there was no statistical association between sulfur supply and any measured dry matter component. 相似文献
143.
Nathan E.N. Erickson Adam Berenik Herbert Lardner Stacey Lacoste John Campbell Sheryl Gow Cheryl Waldner John Ellis 《The Canadian veterinary journal. La revue veterinaire canadienne》2021,62(1):37
Bovine respiratory disease (BRD) is an economically important cause of morbidity and mortality in beef calves. Control of BRD is most often addressed through “homologous” vaccination utilizing the same injectable modified-live viral (MLV) vaccine for both priming and boosting. Heterologous prime-boosting uses different routes and antigenic forms for priming and boosting. Three vaccine protocols were compared: an injectable (IJ) MLV (IJ-MLV) group (IJ-MLV priming at ~48 days and boosted with IJ-MLV at weaning), intranasal (IN) MLV (IN-MLV) group (intranasal priming with MLV at ~24 hours, boosted twice with an IJ-MLV), and intranasal killed viral (IN-KV) group (primed with an IN-MLV at ~24 hours, boosted twice with an IJ-KV). Serum antibody concentrations determined by enzyme-linked immunosorbent assays (ELISAs) were compared and the IN-KV group had significantly higher BRSV-specific antibody concentrations after boosting compared with the 2 homologous groups. No differences in BHV-specific antibody concentrations were observed between any of the groups. 相似文献
144.
Mayewski PA Meeker LD Whitlow S Twickler MS Morrison MC Bloomfield P Bond GC Alley RB Gow AJ Meese DA Grootes PM Ram M Taylor KC Wumkes W 《Science (New York, N.Y.)》1994,263(5154):1747-1751
High-resolution, continuous multivariate chemical records from a central Greenland ice core provide a sensitive measure of climate change and chemical composition of the atmosphere over the last 41,000 years. These chemical series reveal a record of change in the relative size and intensity of the circulation system that transported air masses to Greenland [defined here as the polar circulation index (PCI)] and in the extent of ocean ice cover. Massive iceberg discharge events previously defined from the marine record are correlated with notable expansions of ocean ice cover and increases in PCI. During stadials without discharge events, ocean ice cover appears to reach some common maximum level. The massive aerosol loadings and dramatic variations in ocean ice cover documented in ice cores should be included in climate modeling. 相似文献
145.
OBJECTIVE: To evaluate the in vitro antifungal properties of silver sulfadiazine (SSD) and natamycin against filamentous fungi isolated from eyes of horses with keratomycosis. SAMPLE POPULATION: Filamentous fungal isolates obtained from eyes of keratomycosis-affected horses. PROCEDURES: Fungal culture of ocular samples yielded 6 Fusarium spp; 7 Aspergillus spp; and 1 isolate each of Curvularia, Scopulariopsis, Penicillium, and Chrysosporium. For each fungal isolate, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of SSD and natamycin were determined. RESULTS: For all 17 fungal isolates, SSD MIC distribution ranged from < or = 1 to > 64 microg/mL; MIC50 and MIC90 (MICs at which 50% and 90% of organisms were inhibited) were 4 and 32 microg/mL, respectively. The SSD MFC distribution for all isolates was < or = 1 to > 64 microg/mL; MFC50 and MFC90 (MFCs at which 50% and 90% of organisms were killed) were 8 and > 64 microg/mL, respectively. For all fungal isolates, natamycin MIC distribution ranged from 256 to > 1,000 microg/mL; MIC50 and MIC90 were 512 and > 1,000 microg/mL, respectively. The natamycin MFC distribution for all isolates ranged from 512 to > 1,000 microg/mL; MFC(50) and MFC(90) were each > 1,000 microg/mL. CONCLUSIONS AND CLINICAL RELEVANCE: These in vitro data suggest that SSD is fungicidal against the fungal isolates that were obtained from eyes of horses with keratomycosis and that natamycin is fungicidal against some of the isolates at the drug concentrations evaluated. Silver sulfadiazine may be a therapeutic option for equine keratomycosis. 相似文献
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