Crystal structure of a naturally occurring dinucleoside monophosphate: uridylyl (3',5') adenosine hemihydrate

The crystal structure of uridylyl (3',5') adenosine hemihydrate has been analyzed by x-ray diffraction. The two independent molecules found in the asymmetric unit exhibit conformations that differ significantly from those found in double-helical RNA. The conformational information obtained from this analysis provides considerable insight into the possible conformations of nonhelical "loop" regions of transfer RNA, as well as single-stranded regions of nucleic acids in general.     

Long-Term Residual Value of Zinc Fertilizer for Pasture Legumes Using Yield of Dried Herbage and Critical Concentration of Zinc in Young Tissue

The zinc (Zn) requirements of subterranean clover (Trifolium subteraneaum L.) are well known for the predominantly marginally acidic to neutral sandy soils of southwestern Australia, but the Zn requirements of French serradella (Ornithopus sativus Brot.) and biserrula (Biserrula pelecinus L.), now also grown on these soils, is not known and were investigated in a glasshouse pot experiment. Soil was collected from field plots never treated with Zn or treated once only with 0.5 and 1.0 kg Zn/ha as Zn oxide either in 1983, 1984, 1986, 1990, 1992, 1996, 1997, or 2000. In the pot experiment, six levels of Zn were freshly-applied to samples of soil collected from each Zn treatment of the field experiment, and pots were sown either with subterranean clover cv. ‘Dalkeith’, French serradella cv. ‘Margurita’, or biserrula cv. ‘Casbah’. The pasture species were defoliated by cutting pasture herbage 3 cm above the soil surface for up to 4 consecutive defoliations. The approximate linear relationship between the level of freshly-applied Zn in the pot experiment required to produce 90% of the maximum yield of dried defoliated herbage (y-axis) and the number of years since Zn was applied in the field (x-axis) was used to project the number of years it took for the 0.5 or 1.0 kg Zn/ha treatments applied in the field to require the same level of freshly-applied Zn to produce 90% of the maximum yield of the nil-Zn treatment in the field. This was 18–19 years for the 0.5 kg Zn/ha treatment and 32–35 years for the 1.0 kg Zn/ha treatment. The range in values was due to differences between legume species and the defoliation treatments, which were not significant. When yields were measured, samples of young growth (YG) were separated from the rest of the defoliated herbage (ROH) and the concentration of Zn in YG and ROH was measured. The concentration of Zn in YG that was related to 90% of the maximum yield of defoliated dried herbage (critical tissue test value) was (mg/kg) 13 for subterranean clover, 17 for French serradella, and 19 for biserrula; respective critical tissue test values for ROH were 19, 24, and 21 mg/kg. The approximate linear relationship between critical Zn concentration in YG (y-axis) and the number of years since Zn was applied in the field (x-axis) was used to project the number of years it took for the 0.5 and 1.0 kg Zn/ha levels applied in the field to reach the same critical tissue test value as the nil-Zn treatment of the field experiment. This was 17–20 years for the 0.5 kg Zn/ha treatment and 31–37 years for the 1.0 kg/ha treatment, which was similar to the 18–19 and 32–35 years projected using yield data. Before sowing the legumes in the pot study soil samples were collected to measure soil test Zn using the DTPA procedure. The critical DTPA soil test Zn, the soil test value that was related to 90% of the maximum yield of defoliated dried herbage, was about 0.20 mg Zn/kg for the 3 pasture legume species and all Zn treatments in the field and pot studies.     

Soil and Tissue Tests to Predict the Phosphorus Requirements of Canola in Southwestern Australia

Canola (oil seed rape, Brassica napus L.) is now a major crop grown on the predominantly sandy soils in southwestern Australia and knowledge about the phosphorus (P) requirements of the crop in the region is limited. The results of 22 experiments done in the region are reported to determine the relationships between absolute seed (grain) yield response to applied P and (1) soil test P (Colwell sodium bicarbonate procedure) and (2) concentration of P measured in dried shoots at the rosette growth stage. Large grain yield responses to applied P occurred when Colwell soil test P values for the top 10 cm of soil were < 20 mg/kg soil and when concentrations of P in dried shoots were < 3.6 g/kg. The fertilizer P requirements for optimal economic return for canola grain production in the region varied from 10–35 kg P/ha. The 9 different canola cultivars used in the experiments from 1987–2005 had no major effect on the relationship between absolute grain yield response to applied P and soil test P. Application of fertilizer P mostly had no significant effects on either oil or protein concentrations in grain.     

Investigation of Caval Syndrome in Dogs Experimentally Infected With Dirofilaria immitis

Nine of 16 dogs inoculated with 200 infective heartworm larvae developed caval syndrome (CS) of heartworm disease (HWD). There was no difference between dogs that did and did not develop CS with regard to total heartworm burden, burden relative to body weight, or female heartworm burden, indicating that factors other than worm mass are involved in the pathogenesis of CS. Male dogs were twice as frequently affected as females, although this finding was not statistically significant. Dogs afflicted with CS exhibited radiographic, pathologic, and hemodynamic evidence of chronic HWD. In a model of single heartworm exposure, these findings strongly support the theory that CS develops due to retrograde migration of adult worms from the pulmonary arteries and right ventricle to the right atrium and venae cavae. Pulmonary artery pressures were dramatically and significantly greater in dogs with CS (60 +/- 18 torr) as compared to non-CS (30 +/- 4 torr) dogs with equal worm burdens.     

Potentiation of triclabendazole action in vivo against a triclabendazole-resistant isolate of Fasciola hepatica following its co-administration with the metabolic inhibitor, ketoconazole

An in vivo study in the laboratory rat model has been carried out to monitor morphological changes in adult Fasciola hepatica over a 4-day period resulting from co-treatment with triclabendazole (TCBZ) and ketoconazole (KTZ), a cytochrome P450 inhibitor. Rats were infected with the triclabendazole-resistant Oberon isolate of F. hepatica, dosed orally with triclabendazole at a dosage of 10 mg/kg live weight and ketoconazole at a dosage of 10 mg/kg live weight. Flukes were recovered at 24, 48, 72 and 96 h post-treatment (p.t.) and changes to fluke ultrastructure were assessed using transmission electron microscopy (TEM). Results showed an increase in the severity of changes to the fluke ultrastructure with time p.t. Swelling of the basal infolds and the associated mucopolysaccharide masses became more severe with time. Golgi complexes, if present, were greatly reduced in size and number by 96 h p.t., and sub-tegumental flooding was seen from the 72 h time-period onwards. Some sloughing of the tegumental covering over the spines was observed at 96 h p.t. The results demonstrated that the Oberon isolate is more sensitive to TCBZ action in the presence of KTZ than to TCBZ alone, reinforcing the idea that altered drug metabolism is involved in the resistance mechanism. Moreover, they support the concept that TCBZ + inhibitor combinations (aimed at altering drug pharmacokinetics and potentiating the action of TCBZ) could be used in the treatment of TCBZ-R populations of F. hepatica.     

Age differences in infants' attention to patterns of different complexities

Infants 3 weeks, 8 weeks, and 14 weeks old were presented with checkerboard patterns which differed in the number of squares they contained. The older the infants, the more complex was the checkerboard that they looked at longest. This result does not appear to depend on changes in visual acuity or accommodation related to age.     

The contribution of different groups of foodstuffs to the intake of dietary fibre

The intake of Dietary Fibre (which is defined as the sum of lignin and the polysaccharides not hydrolysed by the endogenous secretions of the human digestive tract) is derived from the plant cell walls in the diet and other non-structural polysaccharides either present naturally in foods, or derived from polysaccharide food additives such as gums or algal polysaccharides. The composition of this complex mixture, its chemical and physical properties, and therefore, the physiological effects associated with its ingestion, will vary according to the foods making up in the diet. While techniques for the measurement and characterisation of dietary fibre still call for a considerable amount of methodological development it is possible to obtain reliable estimates for the major components and the overall composition of these components. These show that in fruits and vegetables the total dietary fibre content (on a fresh weight basis) lies between 2 and 5 g/100 g with peas and beans being an exception in having higher contents. The dietary fibre in cereals varies with the cereal and the reate of extraction used; for example, wholemeal wheat flours contain between 12 and 15 g/100 g whereas white wheat flour (72% extraction) contains between 3 and 4 g/100 g. Wheat bran and related products have much higher contents. The proportion of the dietary fibre present as cellulose does not show great variablility but lignin is extremely variable. Fruits and vegetables contain very small amounts of lignin unless they have lignified seeds or special lignified tissues. The non-cellulosic polysaccharides of fruits and vegetables are characteristically rich in uronic acids (from pectic substances) and arabino-galactans. In cereals β-glucans and arabino-xylans predominate and wheat bran and wholemeal wheat and rye flours are particularly rich in arabino-xylans. The amounds and composition of the dietary fibre in foods commonly forming the diet eaten in the United Kingdom will be reviewed and their contribution to the diet as a whole will be discussed. Changes in the pattern of food consumption in the United Kingdom in the past century will be discussed in relation to the effects that these have had on the consumption of dietary fibre.     

Equilibrative nucleoside transporter 3 deficiency perturbs lysosome function and macrophage homeostasis

Lysosomal storage diseases (LSDs) are a group of heterogeneous disorders caused by defects in lysosomal enzymes or transporters, resulting in accumulation of undegraded macromolecules or metabolites. Macrophage numbers are expanded in several LSDs, leading to histiocytosis of unknown pathophysiology. Here, we found that mice lacking the equilibrative nucleoside transporter 3 (ENT3) developed a spontaneous and progressive macrophage-dominated histiocytosis. In the absence of ENT3, defective apoptotic cell clearance led to lysosomal nucleoside buildup, elevated intralysosomal pH, and altered macrophage function. The macrophage accumulation was partly due to increased macrophage colony-stimulating factor and receptor expression and signaling secondary to the lysosomal defects. These studies suggest a cellular and molecular basis for the development of histiocytosis in several human syndromes associated with ENT3 mutations and potentially other LSDs.