Pelger-Huët anomaly in an Arabian horse

Abstract Abstract: A 9‐year‐old Arabian mare was evaluated for a 7‐day history of malaise. Results of a CBC included a leukocyte concentration within the reference interval (8.4 × 10³/μL, reference interval 6.0–14.0 × 10³/μL) with an apparent degenerative left shift (segmented neutrophils 1.2 × 10³/μL, reference interval 2.5–7.5 × 10³/μL; hyposegmented neutrophils 1.8 × 10³/μL, reference interval 0.0–0.2 × 10³/μL). Serum clinical chemistry results included increased aspartate transaminase, alkaline phosphatase, and gamma‐glutamyltransferase activities. A presumptive diagnosis of hepatitis or cholangiohepatitis was made. The horse was treated with antimicrobials and the malaise quickly resolved. However, in a recheck CBC on day 13, the apparent degenerative left shift remained. Further evaluation of the blood smear revealed many hyposegmented granulocytes with coarse mature chromatin and normal cytoplasmic features. On the basis of the microscopic examination, the horse was diagnosed with Pelger‐Huët anomaly. The patient's offspring was subsequently also diagnosed with Pelger‐Huët anomaly on the basis of blood film examination. Neutrophil, eosinophil, and basophil mean nuclear scores in both affected horses (mare, range 1.5–2.6; offspring, range 1.6–3.2) were lower than those in 2 unrelated Arabian horses (range, 2.8–5.0) and 5 non‐Arabian control horses (range, 2.8–5.0). Results of immunophenotyping and phagocytosis/oxidative burst assays via flow cytometry showed no difference in the expression of myeloid–specific or adhesion molecules or in neutrophil function between affected and control horses. This is the second known report of equine Pelger‐Huët anomaly, both of which affected Arabian horses.     

Farmers’ Perceptions and Actions to Decrease Crop Raiding by Forest-Dwelling Primates Around a Rwandan Forest Fragment

Human–wildlife conflict (HWC) presents an increasing challenge to conservation, particularly in densely populated parts of low-income countries. This article assessed one form of conflict, crop raiding by forest-dwelling primates, along the boundary of a tropical montane forest fragment (Gishwati Forest) in western Rwanda. Variation in species involved, crops affected, differences in magnitude with season and distance to the forest boundary, and the nature of mitigation efforts were assessed through semi-structured interviews with farmers and field-based observations during June and July 2009. Substantial losses of crops were reported, with replacement costs possibly reaching 10–20% of total household income. Chimpanzees (Pan troglodytes) and Cercopithecus monkeys were the sole reported raiders, mainly affecting maize and legumes. Mitigation was restricted to guarding of crops and modification of farming practices, the latter potentially having significant dietary consequences for subsistence farmers. These findings highlight the potentially high economic costs, and food security effects of HWC.     

Hemolysis and Heinz body formation associated with ingestion of red maple leaves in two alpacas

Two adult female alpacas were evaluated for acute onset of weakness, pale mucous membranes, and signs of depression of unknown etiology. Both alpacas had intravascular hemolysis, anemia, and Heinz body formation and had been fed wilted red maple leaves. Clinical signs developed several days after ingestion of the leaves. No other toxin exposure was reported, and no parasites were detected. Dietary copper and nitrate-nitrite concentrations were evaluated and determined to be within reference limits. Both alpacas continued to become profoundly anemic with continued hemolysis. This necessitated blood transfusion as a life-saving intervention; however, prior to whole blood being available, transfusion with bovine hemoglobin glutamer was necessary. Both alpacas recovered without complications.     

欧盟对禽流感定义的变化

近年来的经验表明，中低致病性的禽流感病毒可很容易地突变成为高致病性病毒，因此，现在对所有禽流感病毒的检测工作必须认真对待。欧盟已经转而对禽流感本身重新定义，这项行动可能对国际贸易产生深远的影响。     

Preliminary in vivo study on the potential application of a novel method of e-tracking to facilitate traceability in the poultry food chain

The feasibility of using GS1 DataMatrix (GS1 DM) barcodes laser printed onto the beaks of poultry as a possible method of identification and, therefore, traceability of the individual were examined in this study, including a preliminary live trial on layer hens. The optimal laser type and settings for this particular application had been selected during previous in vitro and in vivo trials. GS1 DM barcodes were printed on both sides of the beaks of mature layer hens and read using a high specification camera based 1-Dimensional/2-Dimensional (1-D/2-D) DataMan 7500 barcode reader. The reading procedure was repeated on a number of occasions over a 5 week period to examine the effects of time in a commercial environment on the clarity and readability of the GS1 DM barcode, and the ability of the printed GS1 DM barcodes to resist the physical and chemical challenges of such a setting. The results show a very short timeframe during which all barcodes, both right and left combined, remain readable. Thereafter the readability deteriorates rapidly, due to the growth and healing of the beaks of the layer hens. Results also show that there was no significant difference in the readability between GS1 DM barcodes printed on the right or left side of the beak. The proportion of identification (ι) (i.e. number of layers identifiable by either one or two readable GS1 DM barcodes) was also calculated. All layer hens were fully identifiable for a seven day period by either one or two readable GS1 DM barcodes. Further analysis showed that the proportion of identification (ι) was significantly higher for layer hens when identified with two GS1 DM barcodes as opposed to just one. Secure movement control of live mature poultry at vulnerable points in the food chain, such as transfer of ownership, could well be facilitated by the use of this technology, thereby preventing fraud or substitution at these points.     

Interstate Equine Semen and Embryo Shipment Regulations in the United States and Their Implications on Control of Disease Transmission

Outbreaks of both equine viral arteritis and contagious equine metritis (CEM) in the United States have occurred in recent years. Shipped semen has been implicated in disease transmission and rapid spread. A survey was performed of state regulations regarding testing for equine arteritis virus (EAV) and CEM before interstate semen or embryo shipment. Results demonstrated lack of any requirements in 31 states. Four states had regulations regarding EAV; 17 required a Certificate of Veterinary Inspection (CVI) to accompany a semen or embryo shipment. Ten states required a negative equine infectious anemia test, primarily as a requirement of a CVI versus because of risk of dissemination of disease. No states required CEM testing. A comparison of state department of agriculture or veterinary medical association Web sites and direct communication with state veterinarians or their offices demonstrated contradictory information in six states. The lack of uniform regulations concerning CEM and EAV testing for shipped semen and embryos, and the threat they pose to the equine breeding industry and horse health, should be alarming to veterinarians and horse owners. Routine testing of animals before shipment of semen or embryos and veterinary involvement in collection and breeding activities are appropriate and necessary to help prevent future outbreaks and protect equine health.     

Cardio-Protective Properties and Health Benefits of Fish Lipid Bioactives; The Effects of Thermal Processing

The beneficial effects of fish-derived lipid bioactives have come to prominence over the last few decades, especially for their utilization in fish oils, supplements, and nutraceuticals. Omega-3 (n-3) polyunsaturated fatty acids (PUFA), lipid vitamins, carotenoids, and polar lipid bioactives from fish have shown to possess a vast range of beneficial effects against a multitude of chronic disorders and especially against inflammation-and cardiovascular disorders (CVD). The observed cardio-protective effects and health benefits are believed to be attributed to the synergy of these fish-derived lipid bioactives. Within the present article the recent findings in the literature on the lipid content of the mainly consumed fish species, their bio-functionality, and cardio-protective benefits is thoroughly reviewed. Moreover, the recovery and valorization of such lipid bioactives from fish by-products and fishing by-catch, in order to reduce waste, while developing useful products containing cardio-protective lipids from the leftover materials of fisheries and aquaculture industries, are also of industrial and environmental interest. Emphasis is also given to the effects of heat treatments during fish processing on the structures and bio-functionality of these marine lipid bioactives, based on the paradigm of different cooking methodologies and thermal processing, while the compounds produced during such treatment(s) with detrimental changes in the fish lipid profile, which can reduce its cardio-protective efficacy, are also reviewed. Novel green extraction technologies and low temperature processing and cooking of fish and fishery by-products are needed to reduce these undesirable effects in a sustainable and environmentally friendly way.