杂交玉米制种基地管理存在的问题和对策

本文阐述了全国杂交玉米制种集中区制种基地发展的现状，分析了基地管理中存在的问题，提出了加强制种基地管理的意见和建议，就是要制定产业保护政策，做好制种基地规划，加大政府投入，建设国家级标准化制种基地，提升种子管理服务能力，强化基地管理，严格市场准入，加强行业自律，建立制种风险政策性保险，开通种子运输“绿色通道”，促进种子产业又快又好发展。     

莱航鸡rDNA重复基因家族的克隆

动物rDNA基因是一种GC含量较高、结构复杂的重复序列。通过结合生物信息学技术,经反复摸索后选用LAPCR法扩增莱航鸡rDNA基因重复序列,经测序鉴定最终克隆了莱航鸡的3个rDNA基因及其2个间隔序列。研究对克隆复杂DNA序列时引物设计的特别规则、反应体系的改进、DNA聚合酶的选用、循环参数的调整等进行了探索。     

森林资源社区共管的经济学分析

森林资源社区共管作为一种将森林资源生态生产力的发展和经济生产力的提高相结合的全过程管理方式,得到了社会各界的广泛认可.从经济学角度分析,支撑社区共管发展的主要理论包括初始阶段的产权安排理论、计划阶段的资源配置理论及审批实施阶段的监测评估理论.就我国目前森林资源管理的实践而言,社区共管的本土化已成趋势,提高其中的"耦合度",必须保障集体产权、强化政府能力,建立激励机制、改变管理体制,建立参与式动态监测评估系统.     

S_m∨P_n和S_m∨C_n的边共色数

利用Lowell Beineke和Richard Ringeisen给出的边共色数的界,研究得到了Sm∨Pn和Sm∨Cn的边共色数.     

小波神经网络在模拟电路故障诊断中的应用研究

对模拟电路提出了一种基于小波与神经网络辅助式结合的故障诊断方法．该方法用小波变换作为模拟电路故障信号的预处理器，大大减少神经网络的输入数目，简化神经网络结构和减少它的训练时间，提高辨识故障能力．在介绍该故障诊断方法的基本原理后。给出了小波函数及故障特征选择的方法．     

Mechanism of systemic inflammatory response syndrome induced by EC DNA

AIM: To investigate whether the bacterial DNA participates in SIRS and its possible mechanism. METHODS: Escherichia coli genomic DNA (EC DNA) was extracted and purified from Escherichia coli 25922 by alkaline lysis method. Mortality of mice challenged with EC DNA and the changes of TNF-α and IL-6 in rat serum were observed. ANA-1 cells were cultured in vitro, after the cells were stimulated by different concentrations of EC DNA and LPS, the level of TNF-α and IL-6 in supernatant were tested. Meanwhile,expression of TLR9 and TLR4 on cell surface was measured. Activation of NF-JB was also observed. RESULTS: The lethal effect of EC DNA on mice with an obvious dose-effect relationship was observed. The death happened within 24 hours. Calf thymus DNA and DNase I-treated EC DNA did not lead to mice to die. The changes of serum TNF-α and IL-6 in rats induced by EC DNA and LPS were similar, but TNF-α peak level of EC DNA group appeared 1 hour earlier than that of LPS group. In vitro, large amount of TNF-α and IL-6 were released from ANA-1 cells stimulated by EC DNA. High expression of TLR9 and TLR4 was observed on surfaces of THP-1 cells. In particularly, LPS induced strong activation of NFκB. The results suggested other pathway possibly took part in the signal transduction inducea by EC DNA. CONCLUSION: EC DNA has the abilities to lead to death of mice, andinduces serum TNF- αand IL- 6 level to increase in rats and ANA- 1 cells to release cytokines in vitro. High expression of TLR9 and TLR4, strong activation of NF- κB may be its importantmolecular mechanism, but other pathway probably exists to play an important role.     

Attempted transmission ofBabesia major byBoophilus microplus

Tropical Animal Health and Production - Two experiments were carried out to determine ifBabesia major could be transmitted byBoophilus microplus. In experiment 1, aBabesia-free batch of laboratory...     

提高丘陵岗地楸树造林成活率的技术措施

以2 a生楸树实生苗为试验材料,研究了腐殖酸液肥、生物菌肥、保水剂和生根粉技术对提高丘陵山区困难立地造林成活率的应用效果.结果表明:在栽植穴内施入腐殖酸液肥、施用抗旱保水剂或使用GGR生根粉溶液对根系进行浸泡处理,均能显著提高楸树在困难立地上的造林成活率;在栽植穴内填加生物菌肥,对造林成活率没有显著影响.试验中,每株施用2.5 kg腐殖酸液肥或施用200 g抗旱保水剂,造林成活率最高;此外,使用1000 mg/L的GGR1号生根粉溶液浸泡苗木根系2 min,造林后成活率最高.