全文获取类型
收费全文 | 239篇 |
免费 | 9篇 |
国内免费 | 2篇 |
专业分类
林业 | 5篇 |
农学 | 9篇 |
基础科学 | 1篇 |
22篇 | |
综合类 | 14篇 |
农作物 | 50篇 |
水产渔业 | 27篇 |
畜牧兽医 | 109篇 |
园艺 | 4篇 |
植物保护 | 9篇 |
出版年
2022年 | 3篇 |
2021年 | 12篇 |
2020年 | 12篇 |
2019年 | 6篇 |
2018年 | 3篇 |
2017年 | 10篇 |
2016年 | 15篇 |
2015年 | 20篇 |
2014年 | 11篇 |
2013年 | 9篇 |
2012年 | 12篇 |
2011年 | 25篇 |
2010年 | 10篇 |
2009年 | 15篇 |
2008年 | 20篇 |
2007年 | 15篇 |
2006年 | 9篇 |
2005年 | 9篇 |
2004年 | 16篇 |
2003年 | 6篇 |
2002年 | 3篇 |
2000年 | 1篇 |
1997年 | 1篇 |
1994年 | 1篇 |
1985年 | 2篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1974年 | 2篇 |
排序方式: 共有250条查询结果,搜索用时 15 毫秒
81.
82.
83.
In most sciaenids, males possess sonic muscles and produce sound through the contraction of these muscles and amplification
of the swim bladder. The sonic muscles in some fishes exhibit seasonal changes in size. For example, they are hypertrophic
in the spawning season, and atrophic in the non-spawning months. The protein profiles of the sonic muscle, red muscle, and
white muscle in the Johnius macrorhynus were shown by two-dimensional electrophoresis (2-DE) and were compared to reveal differential protein expressions. About
80 up-regulated protein spots in the sonic muscle, and 30 spots related to six contractile proteins (fast muscle myosin heavy
chain, skeletal alpha actin, alpha actin cardiac, tropomyosin, myosin light chain 2, and myosin light chain 3), four energy
metabolic enzymes (enolase, acyl-CoA synthetase, creatine kinase, and cytochrome P450 monooxygenase), and two miscellaneous
proteins (DEAD box protein and cyclin H) were identified. Seasonal hypertrophy and atrophy of the sonic muscles related to
the reproductive cycle were verified in male big-snout croaker. The contents of some proteins were significantly different
in the muscles under these conditions. The levels of cytochrome P450 monooxygenase, fast muscle myosin heavy chain, DEAD box
proteins, isocitrate dehydrogenase, and creatine kinase were up-regulated in the hypertrophic muscle, but the levels of alpha
actin cardiac, myosin light 2, and myosin light 3 were lower than in the atrophic muscle. Potential reasons for these differences
in protein expression related to physiological adaptation are discussed. 相似文献
84.
Lee BM Han YW Kim SB Rahman MM Uyangaa E Kim JH Roh YS Kim B Han SB Hong JT Kim K Eo SK 《Comparative immunology, microbiology and infectious diseases》2011,34(4):369-380
The enhanced effect of cytokine combinations has been assessed empirically, based on their immunobiological mechanisms. However, far less is known of the enhanced protection of practical cytokine combinations against viral infection in the livestock industry, due to cost and production issues associated with mass administration. This study demonstrates the enhanced protection of oral co-administration of swine interferon-α (swIFN-α) and interleukin-18 (swIL-18) against infection with transmissible gastroenteritis virus (TGEV) in piglets using attenuated Salmonella enterica serovar Typhimurium as carrier of cytokine proteins. A single oral co-administration of S. enterica serovar Typhimurium expressing swIFN-α and swIL-18 induced enhanced alleviation of the severity of diarrhea caused by TGEV infection, compared to piglets administered S. enterica serovar Typhimurium expressing swIFN-α or swIL-18 alone. This enhancement was further observed by the reduction of TGEV shedding and replication, and the expression of IFN-stimulated gene products in the intestinal tract. The results suggest that the combined administration of the swIFN-α and swIL-18 cytokines using attenuated S. enterica serovar Typhimurium as an oral carrier provides enhanced protection against intestinal tract infection with TGEV. 相似文献
85.
Kim EJ Kwon J Park SH Park C Seo YB Shin HK Kim HK Lee KS Choi SY Ryu do H Hwang GS 《Journal of agricultural and food chemistry》2011,59(16):8806-8815
Angelica gigas obtained from different geographical regions was characterized using (1)H nuclear magnetic resonance (NMR) spectroscopy and ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) followed by multivariate data analyses. Principal component analysis (PCA) and orthogonal partial least-squares-discriminant analysis (OPLS-DA) score plots from (1)H NMR and UPLC-MS data sets showed a clear distinction among A. gigas from three different regions in Korea. The major metabolites that contributed to the discrimination factor were primary metabolites including acetate, choline, citrate, 1,3-dimethylurate, fumarate, glucose, histamine, lactose, malate, N-acetylglutamate, succinate, and valine and secondary metabolites including decursin, decursinol, nodakenin, marmesin, 7-hydroxy-6-(2R-hydroxy-3-methylbut-3-ethyl)coumarin in A. gigas roots. The results demonstrate that (1)H NMR and UPLC-MS-based metabolic profiling coupled with chemometric analysis can be used to discriminate the geographical origins of various herbal medicines and to identify primary and secondary metabolites responsible for discrimination. 相似文献
86.
Ju‐Hwan LEE Ichiro YAMAMOTO Jin‐Suk JEONG Toshihiro NADE Toshiro ARAI Nobuhiro KIMURA 《Animal Science Journal》2011,82(5):689-697
The amount of monounsaturated fatty acid (MUFA) is intimately related to adipose softness, melting point (MP) and flavor in beef. Stearoyl‐CoA desaturase (SCD) is a main gene involved in MUFA synthesis. Mature adipose tends to be highly saturated, whereas immature or maturing adipose is highly unsaturated when chronologically based, so the degree of non‐saturation can be an index of adipose maturity. In this study, three different adipose tissues (coelomic (CL), perirenal (PR), and subcutaneous (SC)) from three beef breeds with differing slaughter ages (Japanese Black (29.5 months), Holstein (20.1 month), and F1 crossbreed (25.6 months)) were examined to: (i) determine adipose maturity level as indexed by MUFA %; and (ii) determine SCD and other lipogenic gene messenger RNA (mRNA) expression levels in relation to unsaturated fatty acid content. Fatty acid composition was significantly different between adipose tissues (P < 0.05). MUFA amount was high in the following order: SC > CL > PR. This pattern corresponded to SCD mRNA expression profile showing higher expression in SC than CL and PR. However, Japanese black cattle are an exception with CL adipose containing similar UFA % as SC adipose, yet having the lowest SCD mRNA expression level among all adipose tissues tested. Therefore, SCD mRNA expression and MUFA % appear to be directly related; however, differences in SCD mRNA expression among three adipose tissues may reflect differences in the fat development characteristics affected by chronological age of the cattle breeds. 相似文献
87.
Kim YK Lee SS Suh EH Lee L Lee HC Lee HJ Yeon SC 《Journal of zoo and wildlife medicine》2011,42(3):499-503
This study aimed to determine the minimum anesthetic concentration (MAC) and dose-related cardiovascular effects of isoflurane during controlled ventilation in cinereous vultures (Aegypius monachus). The MAC was determined for 10 cinereous vultures as the midpoint between the end-tidal isoflurane concentration that allows gross purposeful movement and that which prevents the movement in response to clamping a pedal digit. Immediately after the MAC was determined, the cardiovascular effects of isoflurane at 1.0, 1.5, and 2.0 times the MAC were investigated in seven of the 10 birds. The MAC of isoflurane for 10 cinereous vultures during controlled ventilation was 1.06 +/- 0.07% (mean +/- SD). When the isoflurane concentration was increased to 1.5 and 2.0 times the MAC, there was significant dose-dependent decrease in the arterial blood pressure. However, the heart rate did not change over a range of 1.0 to 2.0 times the MAC. 相似文献
88.
Dong Woo Chang Bora Kim Jae Hoon Shin Young Min Yun Jung Ho Je Yeu kuang Hwu Jung Hee Yoon Je Kyung Seong 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(2):107-113
The X-ray synchrotron is quite different from conventional radiation sources. This technique may expand the capabilities of conventional radiology and be applied in novel manners for special cases. To evaluate the usefulness of X-ray synchrotron radiation systems for real time observations, mouse fetal skeleton development was monitored with a high resolution X-ray synchrotron. A non-monochromatized X-ray synchrotron (white beam, 5C1 beamline) was employed to observe the skeleton of mice under anesthesia at embryonic day (E)12, E14, E15, and E18. At the same time, conventional radiography and mammography were used to compare with X-ray synchrotron. After synchrotron radiation, each mouse was sacrificed and stained with Alizarin red S and Alcian blue to observe bony structures. Synchrotron radiation enabled us to view the mouse fetal skeleton beginning at gestation. Synchrotron radiation systems facilitate real time observations of the fetal skeleton with greater accuracy and magnification compared to mammography and conventional radiography. Our results show that X-ray synchrotron systems can be used to observe the fine structures of internal organs at high magnification. 相似文献
89.
Choi JH Lee CH Hwang IK Won MH Seong JK Yoon YS Lee HS Lee IS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(11):1131-1136
Microglia are evenly distributed throughout the brain parenchyma. They respond rapidly to a variety of alterations in the microenvironment of the brain and act as sensors for pathological events in the brain. In the present study, we investigated the age-dependent changes in the immunoreactivity and protein level of ionized calcium-binding adapter molecule 1 (Iba-1), a microglial marker, in the CA1 region of the gerbil hippocampus. Iba-1 immunoreactive microglia were detected in the hippocampal CA1 region of the postnatal month 1 (PM 1) group. Iba-1 positive microglia were morphologically inactive between the PM 1 and PM 12 stages. Some Iba-1 immunoreactive microglia were present in the active form in the hippocampal CA1 region of the PM 18 and PM 24 groups. The Iba-1 protein levels in hippocampal CA1 homogenates were decreased in the PM 1 through PM 6 groups and increased in an age-dependent manner thereafter. These results suggest that Iba-1 immunoreactive microglia in the active form were detected in the hippocampal CA1 region in the PM 18 and PM 24 groups. This result may be associated with an age-dependent susceptibility to neurodegenerative diseases associated with the hippocampus. 相似文献
90.
Jin HUR Seong Kug EO Sang-Youel PARK Yoonyoung CHOI John Hwa LEE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1693-1696
Salmonella Typhimurium strain expressing the Actinobacillus
pleuropneumoniae antigens, ApxIA, ApxIIA, ApxIIIA and OmpA, was previously
constructed as a vaccine candidate for porcine pleuropneumonia. This strain was a live
attenuated (∆lon∆cpxR∆asd)Salmonella as a delivery host
and contained a vector containing asd. An immunological study of
lymphocyte proliferation, T-lymphocyte subsets and cytokines in the splenocytes of a mouse
model was carried out after stimulation with the candidate Salmonella
Typhimurium by intranasal inoculation. The splenic lymphocyte proliferation and the levels
of IL-4, IL-6 and IL-12 of the inoculated mice were significantly increased, and the T-
and B-cell populations were also elevated. Collectively, the candidate may efficiently
induce the Th1- and Th2-type immune responses. 相似文献