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81.
Ki-Soo Park Yong-Soon Lee Kyung-Sun Kang 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(4):343-348
Mesenchymal stem cells (MSCs) have the capabilities for self-renewal and differentiation into cells with the phenotypes of bone, cartilage, neurons and fat cells. These features of MSCs have attracted the attention of investigators for using MSCs for cell-based therapies to treat several human diseases. Because bone marrow-derived cells, which are a main source of MSCs, are not always acceptable due to a significant drop in their cell number and proliferative/differentiation capacity with age, human umbilical cord blood (UCB) cells are good substitutes for BMCs due to the immaturity of newborn cells. Although the isolation of hematopoietic stem cells from UCB has been well established, the isolation and characterization of MSCs from UCB still need to be established and evaluated. In this study, we isolated and characterized MSCs. UCB-derived mononuclear cells, which gave rise to adherent cells, exhibited either an osteoclast or a mesenchymal-like phenotype. The attached cells with mesenchymal phenotypes displayed fibroblast-like morphologies, and they expressed mesenchym-related antigens (SH2 and vimentin) and periodic acid Schiff activity. Also, UCB-derived MSCs were able to transdifferentiate into bone and 2 types of neuronal cells, in vitro. Therefore, it is suggested that the MSCs from UCB might be a good alternative to bone marrow cells for transplantation or cell therapy. 相似文献
82.
Sang-Hyun Lee Jin-Ho Choi Wol-Soo Kim Tae-Ho Han Yong-Seo Park Hirosi Gemma 《Scientia Horticulturae》2006
This study was carried out to investigate the cause of stone cell formation in pear (Pyrus pyrifolia cv. ‘Niitaka’) flesh. Potted plants grown in a glass house were subjected to water stress conditions without irrigation for 30 days from 30 days before full bloom (BFB treatment), full bloom (FB treatment) and 30 days after full bloom (AFB treatment). Control plants were drip-irrigated daily maintaining a soil matrix potential around −40 ± 5 kPa. The formation of stone cells in pear flesh increased in the FB treatment and AFB treatment plants and this tendency was sustained until the harvest season. Root activity was investigated 60 days after full bloom (DAFB) and the triphenyltetrazolium chloride (TTC) reduction potential, the formazan content and leaf water potential were investigated 30, 45, and 60 DAFB. Root activity decreased progressively due to the effect of water stress. Also, the Ca content in leaf and flesh was lower. The peroxidase activity was high in the flesh at the early stages of fruit growth and decreased at the late stages of fruit growth, and then a higher increase of peroxidase activity was observed in water-stressed fruit. The reduction in calcium content of leaf and fruit in plants under water stress may be related to the reduction of root activity and leaf water potential. The increase in peroxidase activity under water stress may be due to limited calcium absorption. Higher peroxidase activity may induce the accumulation of lignin in the cell wall and promote the formation of stone cells in pear flesh. We conclude that water stress condition during the early stages of fruit growth is one of several factors that determine the formation of stone cells in pear flesh. 相似文献
83.
Proliferation and collapse of subcuticular hyphae of Venturia nashicola race 1 were studied ultrastructurally, after inoculation of susceptible Japanese pear cv. Kousui, resistant Japanese pear
cv. Kinchaku, resistant Asian pear strain Mamenashi 12 and nonhost European pear cv. Flemish Beauty leaves, to understand
the nature of the resistance mechanism. After cuticle penetration by the pathogen, the hyphae were observed at lower frequency
in epidermal pectin layers and middle lamellae of leaves of the three resistant plants than in those of susceptible ones.
This result suggested that fungal growth was suppressed in the incompatible interaction between pear and V. nashicola race 1. In the pectin layers of all inoculated plants, some hyphae had modifications such as breaks in the plasmalemma with
plasmolysis, necrotic cytoplasm and degraded cell walls. More hyphae had collapsed in the leaves of the three resistant plants
than in those of the susceptible cv. Kousui. In collapsed hyphae, the polymerized cell walls broke into numerous fibrous and
amorphous pieces, showing that the scab resistance might be associated with cell wall-degrading enzymes from pear plants. 相似文献
84.
C S Park 《Journal of animal science》1985,61(4):924-930
Forty male, Holstein calves were utilized to evaluate effects of either low (12%) or high (25%) dietary protein on growth and serum metabolites, with a particular concern for serum cholesterol. All diets contained 19% acid detergent fiber and consisted of corn silage, alfalfa hay and grain mixtures. Average concentration of total serum cholesterol was depressed (P = .001) for calves on the high-protein diets (58.8 mg/dl) as compared with their low-protein counterparts (67.9 mg/dl). Calves fed the high-protein diet had lower (P = .002) free serum cholesterol but elevated high-density lipoprotein cholesterol as a percentage of total cholesterol. Calves receiving the high-protein diets showed increases (P = .206) in lecithin cholesterol acyltransferase activity compared with low-protein counterparts. Serum urea nitrogen was increased (P = .001) for calves fed high-protein diets, while serum protein and glucose remained unchanged. Calves fed the high-protein diets consumed less dry matter with improved (P = .09) growth efficiency (gain/feed). 相似文献
85.
The antibacterial and antifungal activities of pinosylvin (3,5-dihydroxy-trans-stilbene), a constituent of pine, were studied and compared with those of resveratrol (3,5,4'-trihydroxy-trans-stilbene). Pinosylvin exhibited more potent growth inhibitory activity against Candida albicans and Saccharomyces cerevisiae. 相似文献
86.
在已筛选出的代料香菇胶囊菌种专用配方LSC1和LSC5基础上,设计了7种香菇胶囊菌种培养基配方,以L26为供试菌株,观察测试了菌丝生长速度、瘤状突起物形成时间与数量、菌种成型及在愈伤培养时的特征以及菌种持水性等特征指标,筛选出了适宜于工厂化繁育香菇胶囊菌种的专用培养基配方LSCG2T和LSCG3,并在胶囊菌种工厂化繁育生产线中进行了应用试验,取得了良好效果。试验结果表明,工厂化繁育胶囊菌种的配方中粗细木屑的比例应提高到5∶1,配方中应添加以提供微量元素和基质物理性能改良剂的辅料。 相似文献
87.
The road effect on small rodent population is investigated at 8 fragmented forest areas in the Baekdudaegan moun-tain range, South Korea in September 2001, We especially focused on the distribution and body condition of small rodents near the roads, Korean field mouse (Apodemus peninsulae) seems to be more sensitive to the existence of a road than striped field mouse (Apodemus agradus), Korean field mouse prefers interior forest area to around road. Striped field mouse is a habitat generalist and has wide distributional range around road, but Korean field mouse is forest-inhabiting species and their distribu-tion is limited in forest area. These results suggest the effect of road is different on each small rodent species and their habitat preferences. 相似文献
88.
In Stenocereus queretaroensis (Weber) Buxbaum, an arborescent cactus cultivated in Jalisco, Mexico, for its fruits but studied here in wild populations, stem extension occurred in the autumn at the beginning of the dry season, flowering and fruiting occurred in the spring at the end of the dry season, and new roots grew in the summer during the wet season. The asynchrony of vegetative and reproductive growth reduces competitive sink effects, which may be advantageous for wild populations growing in infertile rocky soils. Seasonal patterns of sugars in the roots and especially the stems of S. queretaroensis were closely related to the main phenological stages, becoming lower in concentration during periods of major stem extension. Cessation of stem extension occurred in 100-year-old plants for which injection of GA(3) reinitiated such growth. Isolated chlorenchyma cylinders had maximum extension in a bathing solution containing 0.1 &mgr;M gibberellic acid. 相似文献
89.
浅议贵州森林旅游的深层次开发 总被引:1,自引:0,他引:1
在分析旅游文化与贵州森林旅游深层次开发相互关系的基础上,结合贵州森林旅游业发展的实际情况,阐述了贵州森林旅游深层次开发的必要性和可行性,提出贵州森林旅游深层次开发的设想和保障措施。 相似文献
90.
A silvoarable experimental site in Buckinghamshire, England combined rows of poplar trees with an arable rotation. Soil properties were investigated using soil carbon and the bodysize of invertebrates. Four years after establishment, the soil within and 1 m from the tree rows has significantly higher soil organic matter and invertebrate populations. These effects do not extend far beyond the crown diameter. 相似文献