Diagnosis of vibriosis in bulls at breeding stations through lavages of the artificial vagina]

The objective of the study was to test the suitability of lavages of artificial vaginas which are used for collecting ejaculate for diagnosing the Campylobacter fetus subsp. fetus germs. According to the results of the investigation this method may be considered equal to the method of lavages of the prepuce. However, it is important to point out the advantages: it eliminates the danger of an accident, it enables quality lavage in the laboratory. To caputure the Campylobacter fetus the filtration through diaphragm filters is used together with the culture medium Fluid Thioglycollate medium (Difco) with an additive of agar, 10% ram blood and antibiotics (bacitracin, novobiocin). The catch rate in the prepuce lavages is 26.9%, in lavages of the artificial vagina 26.1%. The insignificant difference between the errors of the methods, i.e. the number of negative findings by employing one method and positive results with the other method, is 24.4% in lavages of the artificial vagina and 22.0% in lavages of the prepuce.