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71.
Satoshi KAMOTO Masahiro SHINADA Daiki KATO Masaya TSUBOI Sho YOSHIMOTO Ryohei YOSHITAKE Shotaro ETO Namiko IKEDA Yosuke TAKAHASHI Yuko HASHIMOTO James CHAMBERS Kazuyuki UCHIDA Shinji YAMADA Mika K. KANEKO Ryohei NISHIMURA Yukinari KATO Takayuki NAKAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1795
Podoplanin is expressed in various human tumors where it promotes tumor progression, epithelial-mesenchymal transition, and distant metastasis. Podoplanin is also expressed in cancer-associated fibroblasts and induces tumor malignancy. The objective of this study was to evaluate podoplanin expression in various types of feline tumor tissues. Immunohistochemical analysis revealed that podoplanin was expressed in cells of 13/15 (87%) squamous cell carcinomas and 5/19 (26%) fibrosarcomas. Moreover, cancer-associated fibroblasts expressed podoplanin in most tumor types, including 18/21 (86%) mammary adenocarcinoma tissues. Our findings demonstrate that various types of feline tumor tissues expressed podoplanin, indicating the importance of the comparative aspects of podoplanin expression, which may be used as a novel research model for podoplanin biology. 相似文献
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Takayuki YAMAMOTO Masatoshi KATO Kiwamu ENDO Satoshi KOTOURA Zenya TAKEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1605-1609
A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) using two
monoclonal antibodies directed against a synthetic peptide with an amino-acid sequence
related to the C-terminus of bovine myoglobin and the whole molecule of sodium dodecyl
sulphate (SDS)-denatured bovine myoglobin was adapted for detecting bovine myoglobin in
contaminated feeds. The ELISA employed bovine meat extract of a known myoglobin
concentration as a calibration standard and had an limit of detection (LOD) of 3.54
ng/ml and an limit of quantification (LOQ) of 11.0
ng/ml corresponding to 0.022% and 0.067% (wt/wt)
bovine meat-and-bone-meal (MBM) mixed in 20-fold-diluted feed extracts, respectively. A
cut-off threshold of 20.6 ng/ml bovine myoglobin was set
to simplify ELISA and facilitate quick assessment of test results without a tedious
calibration process. The ELISA was able to detect bovine MBM in artificially prepared
model feeds, mixed botanical feeds, mixed botanical feeds with skimmed milk, fish meal,
pork meal and pork/chicken meal at 0.1% (wt/wt). It was also able to detect sheep MBM in
test feeds, but showed no reactivity to swine MBM, chicken MBM, skimmed milk or gelatine
of bovine origin. The advantages of this method are the quick and easy extraction protocol
of proteins from test feeds, using 100 mM sodium sulphide and 0.6% sodium dodecyl sulphate
in the extraction solution and the effective detection of bovine and sheep MBM at 0.1%
(wt/wt). 相似文献
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Akira GOTO Masaaki TAGAMI Fumiki KATO Tsukasa SUZUKI Takashi YAMAGA Harutaka MURASE Fumio SATO Munkhtuul TSOGTGEREL Tadamasa NIIKURA Tomoe MORIYAMA Akiko CHIBA Ken-ichi WATANABE Nao TSUZUKI Yasuo NAMBO 《Journal of Equine Science》2021,32(4):147
We performed a standing hand-assisted laparoscopic ovariectomy in a draft mare that presented with high serum anti-Müllerian hormone (AMH) level and had an enlarged single cystic ovary. Histopathological examination revealed no tumor cell proliferation in the ovary, but the presence of a large ovarian cyst was confirmed. In the diagnosis of abnormal ovaries in mares, a comprehensive assessment should be performed, including the monitoring of ovarian morphology and biomarkers over time, to determine the disease prognosis and treatment plan. The case of this mare with a nonneoplastic abnormal ovary and increased serum AMH level was rare. We suggest that standing hand-assisted laparoscopic ovariectomy is useful for the removal of large ovaries in draft mares. 相似文献
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An experiment was carried out to study the transport process of nitrogen (N) assimilation from tea roots by monitoring the dynamic composition of N compounds in xylem sap after 15^N-NO3 and 15^N-NH4 were fed to the root of tea plants (Camellia sinensis L.). Results showed that the main amino acids were glutamine, theanine, axginine, asparic acid and glutamic acid, which accounted for 49%, 17%, 8%, 7%, and 4%, respectively, of the total amino acids in the xylem sap. After the tea plants were fed with 15^N-NO3 and 15^N-NH4 for 48 h, the amount of total amino acids in xylem sap significantly increased and those fed with 15^N-NH4 had higher increment than those with 15^N-NOa. Two hours after 15^N- NO3 and 15^N-NH4 were fed, 15N abundance in glutamine, asparagine, glutamic acid, alanine, and arginine were detected and increased quickly over time. This indicated that it took less than 2 h for NO3-N and NH4-N to be absorbed by tea roots, incorporated into the above amino acids and transported to the xylem sap. Rapid increase in 15^N-NO3 in the xylem sap of tea plants fed with 15^N-NO3 indicated that nitrate could be directly transported to the xylem sap. Glutamine, theanine, and alanine were the main amino acids transported in xylem sap of tea plants fed with both 15^N-NO3 and 15^N-NH4. 相似文献
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Yuko HARUTA-ONO Hiroshi UENO Noriko UEDA Ken KATO Toshimitsu YOSHIOKA 《Animal Science Journal》2012,83(2):178-183
We previously found that dietary sphingomyelin (SPM) concentrate from bovine milk improved epidermal function. In this study, we investigated the dosage of dietary SPM concentrate from bovine milk in relation to the improvement of epidermal function. Thirteen-week-old hairless male mice were separated into four experimental groups, each fed one of four types of experimental diet: the control group, the low SPM group, the medium SPM group and the high SPM group. The mice were each fed the experimental diet for 6 weeks. The stratum corneum hydration and the transepidermal water loss (TEWL) were measured using a Corneometer and a Tewameter at 3 weeks and 6 weeks. After the feeding period, ceramides in the stratum corneum were analyzed. We found that the stratum corneum hydration in all the SPM groups was significantly higher than that in the control group, whereas TEWL in all the SPM groups was significantly lower than that in the control group. Ceramides increased significantly in mice fed the medium SPM diet and statistically tended to increase in mice fed the high SPM diet. Our results indicate that a daily intake of 17 mg SPM concentrate is enough to improve epidermal function in hairless mice. 相似文献
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Yamato SAJIKI Satoru KONNAI Reiko NAGATA Satoko KAWAJI Hayato NAKAMURA Sotaro FUJISAWA Tomohiro OKAGAWA Naoya MAEKAWA Yukinari KATO Yasuhiko SUZUKI Shiro MURATA Yasuyuki MORI Kazuhiko OHASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):162
Johne’s disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic enteritis of ruminants. Previous studies have shown that programmed death-ligand 1 (PD-L1) is associated with the disease progression, and PD-L1 blockade activates MAP-specific Th1 responses in vitro. Here, we performed anti-PD-L1 antibody administration using 2 MAP-infected cattle at the late subclinical stage of infection. After administration, bacterial shedding was reduced or maintained at a low level. Additionally, MAP-specific Th1 cytokine production was upregulated, and CD69 expression was increased in T cells. Collectively, the treatment has a potential as a novel control method against Johne’s disease. 相似文献
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