全文获取类型
收费全文 | 367篇 |
免费 | 2篇 |
国内免费 | 1篇 |
专业分类
林业 | 41篇 |
农学 | 7篇 |
基础科学 | 3篇 |
47篇 | |
综合类 | 12篇 |
农作物 | 2篇 |
水产渔业 | 44篇 |
畜牧兽医 | 196篇 |
园艺 | 3篇 |
植物保护 | 15篇 |
出版年
2020年 | 2篇 |
2019年 | 1篇 |
2018年 | 2篇 |
2016年 | 5篇 |
2014年 | 7篇 |
2013年 | 25篇 |
2012年 | 19篇 |
2011年 | 27篇 |
2010年 | 8篇 |
2009年 | 18篇 |
2008年 | 29篇 |
2007年 | 28篇 |
2006年 | 30篇 |
2005年 | 28篇 |
2004年 | 22篇 |
2003年 | 25篇 |
2002年 | 19篇 |
2001年 | 18篇 |
2000年 | 14篇 |
1999年 | 12篇 |
1998年 | 3篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1992年 | 2篇 |
1991年 | 4篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1978年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有370条查询结果,搜索用时 15 毫秒
111.
112.
Prevalence of fowl glioma-inducing virus in chickens of zoological gardens in Japan and nucleotide variation in the env gene 总被引:1,自引:0,他引:1
113.
Mekata H Konnai S Simuunza M Chembensofu M Kano R Witola WH Tembo ME Chitambo H Inoue N Onuma M Ohashi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(9):923-928
The prevalence of trypanosome infections in tsetse flies, Glossina pallidipes, collected from Chiawa and Chakwenga in Zambia with endemic trypanosomosis was assessed by polymerase chain reaction (PCR). Out of the 550 G. pallidipes, 58 (10.5%) flies were found to harbor trypanosome DNA. Infection rates of tsetse with Trypanosoma vivax universal, Trypanosoma congolense savannah, T. congolense forest and T. congolense kilifi were 4.2% (23/550), 4.7% (26/550), 1.1% (6/550) and 1.6% (9/550), respectively. To determine the mammalian hosts of T. congolense and T. vivax infections from the tsetse flies, mammalian mitochondrion DNA of blood meal in these flies were analyzed by PCR and subsequent gene sequence analysis of the amplicons. Sequence analysis showed the presence of cytochrome b gene (cyt b) of 7 different mammalian species such as human, elephant, buffalo, goat, warthog, greater kudu and cattle. Goats which were main livestock in these areas were further examined to know the extent of its contribution in spreading the infection. We examined the prevalence of trypanosome infections in the domestic goat population in 6 settlements in Chiawa alone. Of the 86 goats sampled, 4 (4.6%), 5 (5.8%), 4 (4.6%) and 4 (4.6%) were positive for T. vivax universal, T. congolense savannah, forest and kilifi, respectively. These findings showed that the host-source of trypanosome infections in vector fly give a vital information about spread of infection. The result of this study will certainly contribute in elucidating more the epidemiology of trypanosomosis. 相似文献
114.
Marr-Belvin AK Carville AK Fahey MA Boisvert K Klumpp SA Ohashi M Wang F O'Neil SP Westmoreland SV 《Veterinary pathology》2008,45(6):914-921
Epstein-Barr virus (EBV) is a worldwide endemic gamma herpesvirus of the genus Lymphocryptovirus (LCV) that infects more than 90% of the world's population. EBV has been associated with a variety of malignancies, but it has a demonstrated role in lymphomas, especially in immunosuppressed individuals. Lymphomas of the nasal cavity, paranasal sinuses, and nasopharynx are uncommon and constitute less than 5% of all extranodal lymphomas. Sinonasal non-Hodgkin's lymphomas have been reported in patients infected with human immunodeficiency virus (HIV) at an increased frequency. Rhesus LCV (rhLCV), the rhesus viral homolog of EBV, has been cloned and is associated with B-cell lymphomas in immunosuppressed rhesus macaques. We report two cases of B-cell lymphoma within the nasal cavity from 2 simian immunodeficiency virus-infected rhesus macaques with acquired immunodeficiency syndrome. The B-cell phenotype and rhLCV association were demonstrated by immunohistochemistry and confocal microscopy. The majority of the nuclei of the neoplastic B lymphocytes were EBNA-2 positive. RhLCV type 1 sequences were verified from the neoplasms by polymerase chain reaction. Nasal lymphoma is an unusual presentation of rhLCV-associated B-cell lymphoma in immunosuppressed rhesus macaques. These tumors demonstrate comparable viral pathogenesis with EBV-induced nasal lymphomas in HIV-positive people. 相似文献
115.
Takanori Imai Hideyuki Goto Hiroyuki Matsumura Seiichi Yasuda 《Journal of Wood Science》1998,44(2):106-110
myo-Inositol-[2-3H] and d-glucuronic acid-[6-14C] were administered simultaneously to a growing stem of magnolia (Magnolia kobus DC) to label xylan and pectin, respectively, in the cell wall. Determination of the radioactivity of nitrobenzene oxidation products and sulfuric acid hydrolysates of the newly formed xylem indicated that xylan and pectin were labeled with 3H and 14C, respectively. The doubly labeled wood tissue was treated to kraft pulping, and the radioactivity of the pulping black liquor and treated wood tissue were determined at various stages of the pulping to compare the dissolving behavior between pectin and xylan during the pulping. The results showed that pectin was not dissolved as easily as xylan and was not redeposited on pulp fiber at the late stage of the pulping.Part of this report was presented at the 40th Lignin Symposium at Tsukuba, October 1995 相似文献
116.
The chemical conversion of red pine sulfuric acid lignin (Klason lignin) (SAL) as an acid hydrolysis lignin sample to water-soluble arylsulfonates of lignin derivation (i.e., phenolized SAL) was investigated. Treatment of phenolized SAL with chlorosulfonic acid followed by alkali hydrolysis gave water-soluble sulfonated products with a sulfonic acid group on their aromatic nuclei quantitatively. The products possess 2.0 SO3Na/C9 C6. In contrast, the content of sulfuric acid group in sulfonated SAL was only 0.33C9. Chlorosulfonation of 1-guaiacyl-l-p-hydroxyphenylethane as a phenolized guaiacyl lignin model compound revealed that the sulfonyl chloride group was introduced at thepara position of an aromatic methoxyl group, theortho position of a phenolic hydroxyl group, or both. 相似文献
117.
We discovered that some isolated eel skeletal muscle cells exhibited green fluorescence under a fluorescence stereomicroscope,
and we successfully isolated a novel fluorescent protein from the eel muscle homogenate. The protein was a monomer with a
molecular mass of 16.5–17 kDa and showed minor and major peaks at 280 and 493 nm, respectively, in the absorption spectrum.
The molar extinction coefficient at 493 nm was 41,300 M−1 cm−1 and A280/A493 was 0.083. Excitation and emission spectra of the protein showed maxima at 493 and 527 nm, respectively. Heat
treatment at 95°C for 10 min or 5% trichloroacetic acid treatment of the protein caused aggregation of the protein but did
not release any fluorescent components such as FAD into the supernatant after centrifugation. Fluorescence of the protein
remained after native PAGE, but not after SDS-PAGE. These results indicate that the purified fluorescent protein is not a
flavoprotein, and that its fluorescent chromophore is a covalently bound one, such as green fluorescent protein (GFP) from
jellyfish Aequoria victoria, but that its fluorescence requires its native conformation within the protein. Based on these results, we can conclude that
the fluorescent protein obtained from eel skeletal muscle is a novel GFP-like protein. 相似文献
118.
Similarities and differences in meat consumption patterns in Asia and Pacific-Rim countries 总被引:1,自引:0,他引:1
Seiichi KOIZUMI Shinichi KOBAYASHI In-Jen PAN Shinichi TAKAKU Masao BABA Raymond A. JUSSAUME Jr Paul RIETHMULLER Julian MORISON J. C. RYOO C. W. LIN I. H. LIN H. ZHOU Apichai RATANAWARAHA Minoru NAGANO 《Animal Science Journal》2002,73(4):293-300
This study investigated consumer behavior towards meat across Asia and Pacific-Rim countries. Mail surveys were undertaken in 1994 and 1995. Data was obtained from 6197 households in Japan, the USA (mainland and Japanese–Americans in Hawaii), Australia, Thailand, Korea, Taiwan and China. The data were mainly analyzed using cluster analysis to determine the distance between each country and to classify countries into groups. Japanese respondents purchased beef more frequently than respondents from other countries. The average amount purchased on each occasion was far more for Australian respondents than for respondents from other countries, while Japanese purchased far less. Respondents in Japan, Korea, China and Australia thought beef was the most expensive meat, whereas lamb was thought to be the most expensive in the remaining countries. Chinese respondents indicated the highest consciousness of price indexes. The level of uneasiness about eating meat indicated by Asian respondents was relatively strong compared with respondents in Hawaii, the USA and Australia. Average ratings (semantic differential scale) on plans for future meat consumption were higher for beef than for other meats in Korea and China, whereas chicken was rated higher in the other countries. From the dendrogram, surveyed countries were classified into three groups: Japan, Taiwan and Korea; Thailand and China; and the USA, Japanese–Americans and Australia. Among them, the first and second clusters merged into a larger cluster, the 'Asian' group. 相似文献
119.
120.
Matsuu A Koshida Y Kawahara M Inoue K Ikadai H Hikasa Y Okano S Higuchi S 《Veterinary parasitology》2004,124(1-2):9-18
The therapeutic efficacy of atovaquone against Babesia gibsoni was examined in three dogs experimentally infected with B. gibsoni isolated from naturally infected dogs in Aomori Prefecture, Japan. Once parasitemia reached 10%, atovaquone was administered orally (30 mg/kg twice daily for 7 days). Within 2 days of atovaquone treatment, the parasite disappeared from blood smears without any clinical side effects. Anemia and thrombocytopenia were significantly improved in all the dogs. However, a polymerase chain reaction assay revealed that a B. gibsoni marker gene was intermittently present in peripheral blood after atovaquone therapy, indicating that the organism had not been eliminated, and parasites reappeared in blood smears 33 days after the last treatment. To investigate the change in sensitivity against atovaquone, an in vitro sensitivity test was performed using peripheral blood obtained from an untreated dog that was infected with the original parasite isolate, and from two of the experimentally infected and atovaquone-treated animals (blood was collected at the time of the post-treatment recurrence of the B. gibsoni infection). Atovaquone was added to the culture medium to final concentrations of 0.1, 1, 10, 100, and 1000 nM. For the untreated parasites, complete growth inhibition occurred at 1000 nM of atovaquone, whereas the recurrent parasites were inhibited by only 39.52 +/- 8.34% and 31.31 +/- 8.14% at this concentration after 48 h of incubation. Thus, the recurring parasites were less sensitive to atovaquone than the untreated originally isolated parasites. 相似文献