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71.
A unique lipoprotein profile was found in the plasma of cultured Japanese eel,Anguilla japonica which accumulated more lipid in its muscle than in its liver. The plasma lipoprotein level of Japanese eels was in excess of 54 mg ml1, a concentration considered to be hyperlipoproteinemic in relation to other teleosts. The plasma lipoproteins consisted of very low density lipoprotein (VLDL, density (d)<1.006 g ml1) low density lipoprotein (LDL, 1.0061), high density lipoprotein 2 (HDL2, 1.0851), and HDL3 (1.1001). VLDL, but not HDL, was the main component in the plasma of Japanese eels, unlike most teleosts where HDL is the main component. An additional lipoprotein, vitellogenin (1.2101), was induced by the injection of estradiol-17ß (E2), but VLDL was the main plasma component even in the E2-treated eels. VLDL was a triacylglycerol (TG)-rich lipoprotein and possessed two apolipoprotein (apo) B-like proteins of molecular weights (Mr) 260K and 230K as main components.LDL, HDL2, and HDL3 were revealed to consist of heterogeneous components by a density gradient ultracentrifugation. LDL was separated into three subclasses of LDL1 (1.0301), LDL2 (1.0431), and LDL3 (1.0671). LDL1 with apo B-like protein of Mr 230K was a TG-rich lipoprotein, while both LDL2 and LDL3 were cholesterol ester-rich lipoproteins with apo A-I-and A-II-like proteins of Mr 25K and 14K. The particle sizes of HDL2 and HDL3 subclasses differed, although all of HDL2 and HDL3 subclasses possessed apo A-I-and A-II-like proteins of Mr 25K and 14K as main components. To our knowledge, this is the first report of detailed plasma lipoprotein profile in Japanese eels.Corresponding author: till August 18, 1996 and after April 19, 1997: at the above address. From August 19, 1996 till April 18. 1997: c/o Dr. N.H. Haunerland, Department of Biological Sciences. Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6. 相似文献
72.
73.
Kurosaki N Yamato O Sasamoto Y Mori F Imoto S Kojima T Yamasaki M Maede Y 《The Japanese journal of veterinary research》2007,55(1):3-12
In our previous study, it was demonstrated that the administration of anion salts, which slightly lower the dietary cation-anion difference (DCAD), in the prepartum period is safe and effective for preventing milk fever in multiparous cows. In the present study, several clinico-pathological constituents in serum and urine, which might be related to milk fever, were analyzed using stored samples from the previous study to identify clinico-pathological parameters for easily evaluating the efficacy of lowering DCAD and to further investigate the mechanism by which lowering DCAD prevents milk fever. Among the parameters analyzed in the present study, inorganic phosphorus (iP) was involved in milk fever because the serum concentration and urinary excretion of iP were significantly higher in the group of primiparous cows (heifer group), which did not develop hypocalcemia, than those in other groups of multiparous cows. Serum chloride concentrations in the heifer group and the group of multiparous cows fed anion salts (anion group) tended to remain higher than those in other control groups of multiparous cows suggesting that serum chloride concentration may be utilized for evaluating the status of metabolic acidosis and the efficacy of lowerng DCAD in dairy cows fed anion salts. In addition, plasma estradiol-17beta concentration in the heifer group tended to be lower at parturition compared with that in other multiparous groups suggesting that estrogen known as a potent inhibitor of bone resorption may be involved in developing milk fever. 相似文献
74.
Preventive effect of mildly altering dietary cation-anion difference on milk fever in dairy cows 总被引:1,自引:0,他引:1
Kurosaki N Yamato O Mori F Imoto S Maede Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(2):185-192
In the present study, we examined whether mildly altering dietary cation-anion difference (DCAD) contributes to the prevention of milk fever in dairy cows. Thirty multiparous cows and ten primiparous cows (heifer group) were used in this study and the multiparous cows were randomly divided into three groups of ten animals each (anion, non-anion and control groups). The cows in the anion group were given supplemental salts that slightly lowered DCAD. These salts consisted of 115 g of CaCO3, 42 g of CaHPO4, 65 g of MgSO4 x 7 H2O and 80 g of CaCl2 x 2 H2O as a daily dose for each cow, using a catheter from 21 days before the expected date of parturition until parturition. The cows in the non-anion group were given only the same Ca, Mg and ip supplement but no sulfate and chloride salts as that in the anion group. The cows in the control and heifer groups were not given any additional supplement. The incidence of hypocalcemia in the anion group decreased to approximately half of those in the non-anion and control groups, while the heifer group did not develop hypocalcemia at all. In addition, the number of days spent for the treatment of hypocalcemia and the number of drug bottles (calcium borogluconate solution) used for the treatment decreased to less than half in the anion group compared with those in the non-anion and control groups. At parturition, the serum Ca concentration in the control (6.2 +/- 1.9 mg/dl, mean +/- standard deviation) and non-anion groups (6.4 +/- 1.7 mg/dl) were significantly lower than that in the heifer group (8.3 +/- 0.4 mg/dl), and the level in the anion group was intermediate (7.3 +/- 1.3 mg/dl). The change in ionized Ca concentration was almost the same as that in serum Ca concentration, but only the concentration in the anion group tended to increase slightly from a week before parturition and was significantly higher than that in all other groups three days before parturition. Urinary pH in the anion group was maintained at a mildly acidic level (6.8-7.0) for the last two weeks before parturition, compared with those in the control (7.3-7.5) and non-anion groups (7.9-8.1), and similar to that in the heifer group (6.3-7.3). The urinary Ca excretion was the highest in the anion group among all groups during the prepartum period. There were no specific changes in the excretion of parathyroid hormone and 1,25-dihydroxyvitamin D in all groups of multiparous cows while the levels of these hormones remained low in the heifer group throughout the experimental period. The data in the present study indicates that the administration of anion salts that slightly lowered DCAD in the preparum period was effective for preventing milk fever in multiparous cows. Safe and mild metabolic acidosis induced by the anion salts could be evaluated by urinary pH (6.8-7.0), and might increase the responsiveness to Ca requirement at parturition through some complex mechanisms unrelated to the excretion of Ca-related hormones. In addition, it was clarified that primiparous cows have a high potential to respond to sudden Ca demand unrelated to hormone excretion, and their Ca metabolism was in some respects similar to that in multiparous cows fed anion salts. Therefore, manipulating mildly DCAD is expected to be an effective, safe and natural method for preventing milk fever in dairy cows. 相似文献
75.
Nibe K Kita C Morozumi M Awamura Y Tamura S Okuno S Kobayashi T Uchida K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(10):1047-1052
Neuroaxonal dystrophy (NAD) was examined in two Papillon dogs and a mix breed dog between Papillon and Chihuahua. In addition, cerebellar cortical abiotrophy (CCA) in a Papillon dog, which had similar clinical and magnetic resonance imaging (MRI) features to those of NAD, was also investigated. The common clinical symptoms of all dogs affected with NAD and CCA, were pelvic limb ataxia and cerebellar ataxia including intention tremor, head tremor, and hypermetria in the early onset. These clinical signs were progressed rapidly, and two dogs with NAD were euthanized by owner's request and the other two died by aspiration pneumonia. MRI examinations and gross observations at necropsy revealed moderate to severe cerebellar atrophy in all cases of NAD and CCA. The most typical histological change of NAD was severe axonal degeneration with marked spheroid-formation in the dorsal horn of the spinal cords, the nuclei gracilis, cuneatus, olivalis and its circumference in the medulla oblongata. The spheroids were characterized as large eosinophilic or granular globes within the enlarged myelin sheaths, sometimes accompanied by moderate accumulation of microglias and/or macrophages. In contrast, such spheroid formation was minimal in the brain of CCA. In the cerebellum, mild to moderate loss of the Purkinje and granular cells were recognized in three dogs with NAD, whereas these changes were more prominent in a dog with CCA. Although the clinical signs and MRI findings relatively resembled between NAD and CCA, the histopathological features considered to be quite differ, suggesting distinct pathogenesis and etiology. Since both NAD and CCA are proposed as the autosomal recessive hereditary disorders, careful considerations might be needed for the breeding of Papillon and Chihuahua dogs. 相似文献
76.
Koyama Jiro Kitoh Asami Nakai Makoto Kohno Kumiko Tanaka Hiroyuki Uno Seiichi 《Water, air, and soil pollution》2013,224(5):1-9
Water, Air, &; Soil Pollution - Karst systems harbor large groundwater resources for human consumption and represent an important habitat for rare and unprotected specialized animals, the... 相似文献
77.
Yamamoto Y Fukushima T Suna Y Ishii N Saeki A Seki S Tagawa S Taniguchi M Kawai T Aida T 《Science (New York, N.Y.)》2006,314(5806):1761-1764
Controlled self-assembly of a trinitrofluorenone-appended gemini-shaped amphiphilic hexabenzocoronene selectively formed nanotubes or microfibers with different photochemical properties. In these nanotubes, which are 16 nanometers in diameter and several micrometers long, a molecular layer of electron-accepting trinitrofluorenone laminates an electron-donating graphitic layer of pi-stacked hexabenzocoronene. The coaxial nanotubular structure allows photochemical generation of spatially separated charge carriers and a quick photoconductive response with a large on/off ratio greater than 10(4). In sharp contrast, the microfibers consist of a charge-transfer complex between the hexabenzocoronene and trinitrofluorenone parts and exhibit almost no photocurrent generation. 相似文献
78.
Yoshitaka Kubojima Seiichi Kanetani Takeshi Fujiwara Youki Suzuki Mario Tonosaki Hiroshi Yoshimaru Hiroharu Ikegame 《Journal of Wood Science》2008,54(6):443-450
A dead tree of Pinus armandii Franch. var. amamiana (Koidz.) Hatusima (abbreviated to PAAm) was obtained from a natural habitat on Tanega-shima Island and various properties
of its wood were investigated. Grain angle was measured and soft X-ray analysis was undertaken to obtain the density in each
annual ring. Unit shrinkage and dynamic properties were measured by shrinkage, bending, and compression tests. Variations
of wood properties in the radial direction, relationships of wood properties to density, and annual ring width were examined.
Roughly speaking, variations in the radial direction of the grain angle, twist angle by drying, Young’s modulus and strength
in static bending, absorbed energy in impact bending, compressive Young’s modulus, compressive strength, and compressive proportional
limit corresponded to the variation of annual ring width. As a result, it was determined that if PAAm is afforested artificially
for the purposes of lumber production and conservation, the annual rings of logs should not be too widely spaced. Wood properties
of PAAm were similar to those of Japanese black pine (Pinus thunbergii Parl.), which is another representative pine on Tanegashima Island.
This study was presented in part at the 56th Annual Meeting of the Japan Wood Research Society, Hiroshima, August 2007 相似文献
79.
80.
Seiichi Oyamada Yasuhiro Ueno Mitsutaku Makino Koji Kotani Hiroyuki Matsuda 《Fisheries Science》2009,75(2):273-283
In the middle of the 1990s, Pacific saury fishery vessels began to install “size separators” to selectively land large-size
class fish with a higher price. Contrary to expectations, this resulted in removal of the separators in 2006 because fishers
believed the separators had contributed to price collapses in the 2000s. The intent of this paper is to investigate the effects
of separators on both the fishery economy and stock of Pacific saury by simulating population and economic models under a
single framework. For this purpose, we developed (1) an age-structured population dynamics model with stochasticity, and (2)
an economic model spanning both price and inventory dynamics with stochasticity, in which each set of model parameters were
estimated on the basis of time series data. In a 10-year simulation, the harvest quota was set constant from 20,000 to 400,000 t
at intervals of 20,000 t, and the effects of separators were incorporated by controlling the catchability of 0-year-old fish.
We found that separators increase the expected yield and reduce the deficit risk for harvests of 140,000 t and smaller. However,
separators have the opposite effects for harvests of 160,000 t and larger. 相似文献