The influence of soluble carbon and fertilizer nitrogen on nitric oxide and nitrous oxide emissions from two contrasting agricultural soils

Contradictory effects of simultaneous available organic C and N sources on nitrous oxide (N₂O), carbon dioxide (CO₂) and nitric oxide (NO) fluxes are reported in the literature. In order to clarify this controversy, laboratory experiments were conduced on two different soils, a semiarid arable soil from Spain (soil I, pH=7.5, 0.8%C) and a grassland soil from Scotland (soil II, pH=5.5, 4.1%C). Soils were incubated at two different moisture contents, at a water filled pore space (WFPS) of 90% and 40%. Ammonium sulphate, added at rates equivalent to 200 and 50 kg N ha^?1, stimulated N₂O and NO emissions in both soils. Under wet conditions (90% WFPS), at high and low rates of N additions, cumulative N₂O emissions increased by 250.7 and 8.1 ng N₂O–N g^?1 in comparison to the control, respectively, in soil I and by 472.2 and 2.1 ng N₂O–N g^?1, respectively, in soil II. NO emissions only significantly increased in soil I at the high N application rate with and without glucose addition and at both 40% and 90% WFPS. In both soils additions of glucose together with the high N application rate (200 kg N ha^?1) reduced cumulative N₂O and NO emissions by 94% and 55% in soil I, and by 46% and 66% in soil II, respectively. These differences can be explained by differences in soil properties, including pH, soil mineral N and total and dissolved organic carbon content. It is speculated that nitrifier denitrification was the main source of NO and N₂O in the C-poor Spanish soil, and coupled nitrification–denitrification in the C-rich Scottish soil.     

Soil enzyme activity: a brief history and biochemistry as a basis for appropriate interpretations and meta-analysis

Enzyme activity as a method for soil biochemistry and microbiology research has a long history of more than 100 years that is not widely acknowledged in terms of adherence to strict assay protocols and the interpretation of results. However, in the recent past, there is a growing lack of recognition of the historic advancements among researchers that use soil enzymology. Today, many papers are being published that use methods that either do not follow exact protocols as originally vetted in the research literature or individual labs use their own method that has not been optimized for pH, co-factors, substrate concentrations, or other conditions. This is of particular concern for fluorogenic substrates and microplate methods. Furthermore, there is a lack of understanding of the origin and location of a given enzyme being studied. Notably, regardless of the enzyme, it is too often assumed that enzyme activity equals microbial activity—which is not the case for most hydrolytic enzyme assays. Because as established by Douglas McLaren in the 1950s, a considerable amount of activity can come from catalytic enzymes stabilized in the soil matrix but that are no longer associated with viable cells (known as abiontic enzymes). In summary, today, many papers are using imperfect methods and/or misinterpret enzyme activity data that at a minimum confounds cross paper studies and meta-analysis. However, most importantly, lack of historical perspectives and ignoring strict protocols cause redundancy and fundamentally undermine the discipline and understanding of soil microbiology/biochemistry when enzymology methods are used.     

Chemical control of two Phytophthora species infecting the canopy of Monterey pine (Pinus radiata)

Twelve active ingredients were screened for their ability to control foliage disease caused by Phytophthora kernoviae and Phytophthora pluvialis. Inhibition of mycelial growth and sporangial production of both pathogens were assessed in in vitro assays after exposure to three concentrations of each active ingredient. While most of these active ingredients inhibited mycelial growth in vitro, due to their widespread use, phosphite, copper oxychloride and metalaxyl‐M were selected for further study. Four rates of each active ingredient were applied to two Pinus radiata genotypes, and detached needle assays at 6 and 90 days post‐treatment were used to determine treatment efficacy and persistence. Untreated needles showed significantly larger lesions than all fungicide‐treated needles after exposure to P. pluvialis or P. kernoviae on both sampling dates. Efficacy and persistence of the three active ingredients did not increase with increase in concentration. Larger lesions were formed on more susceptible genotype after inoculation with both Phytophthora species, even when higher rates of active ingredients were applied. Phosphite, copper oxychloride and metalaxyl‐M have potential to protect commercially planted P. radiata from these two Phytophthora species.     

Versuche mit Herbiziden in Artischocke (Cynara scolymus L.) in Thüringen

Between 1998 and 2003 11 experiments with 24 herbicides were carried out in artichoke in experimental stations and on artichoke fields of Thuringian farms. The main objective of the tests was to get the necessary data for the authorization procedure according to the regulations of § 18 a PflSchG. Artichoke plants are very susceptible against some herbicides like Afalon, Bandur, Cirrus, Goltix 700 SC, Oratio, Stefes IPU. Other herbicides like Lentagran WP, Stomp Aqua or Stomp Raps can cause severe damages on artichoke if they are applied on unfavourable climatic conditions. The use oft the herbicides BASTA, Devrinol Kombi CS, Gallant Super, Kontakt 320 SC, Lentagran WP, Stomp SC/Stomp Aqua and Targa Super was authorized for the application in artichoke according the regulations of § 18 a PflSchG in the past. The herbicides Fusilade MAX, Kontakt 320 SC and Roundup UltraMAX can be used in artichoke according the regulations of § 18 b PflSchG. These herbicides aren??t enough for the chemical control of weeds likeEchinochloa spec.,Galinsoga spec., Polygonum spec. in artichoke. In addition to the application of the herbicides are mechanical measures necessary.     

Soil chemical attributes,nutrient uptake and yield of no-till crops as affected by phosphogypsum doses and parceling in southern Brazil

This study evaluated soil chemical attributes, leaf nutrient concentrations and grain yield of corn (Zea mays), barley (Hordeum vulgare), common bean (Phaseolus vulgaris) and wheat (Triticum aestivum) as affected by phosphogypsum (PG) rates and split application to a Typic Hapludox under no-till from Southern Brazil. A Randomized complete block design was used, with factorial treatment structure (5 x 2) consisting of increasing PG rates (0, 3, 6, 9 and 12 Mg ha^?1) either in single or split (half dose, two consecutive years) application. PG rates have reduced concentrations of Al³⁺, increasing Ca²⁺, SO₄^2- and pH values in soil layers up to 0.8 m. Leaf concentrations of Ca (between 28 and 42%) and S (between 6% and to 50%) increased in all crops, while leaf levels of Mg decreased on corn (?16%), bean (?22%) and wheat (?14%) by PG rates. Compared to the control, PG rates between 4.0 and 6.1 Mg ha^?1 promoted increases of 11%, 10% and 10% on corn, barley and wheat yields, respectively. There was no effect of PG on common bean’s yield. Phosphogypsum was effective for improving soil fertility, plant nutrition and crop yields. Applying the PG annually at one-half rate gave best overall results.     

United States Department of Agriculture-Agricultural Research Service research on managing insect resistance to insecticides

Insecticide resistance has developed within many classes of pesticide, and over 500 species of insects and mites are resistant to one or more insecticides. Insecticide resistance and the consequent losses of food and fiber caused by failure to control insect and mite pests causes economic losses of several billion dollars worldwide each year. It is the goal of insect resistance management (IRM) to preserve useful pesticides by slowing, preventing or reversing development of resistance in pests. Important aspects of this goal are understanding the development of resistance and monitoring to determine ways to prevent its development. We describe programs specific to missions of the US Department of Agriculture, Agricultural Research Service, which are designed to characterize insecticide resistance in insects and mites with the goal of managing pests in an ecologically acceptable manner. Resistance management of cotton, potatoes, vegetables, melons, ornamentals, greenhouse crops, corn, stored grains, livestock, honeybees and mites, as well as management of transgenic crops are evaluated. We conclude that IRM is a vital part of stewardship of any pest management product and must be a combined effort of manufacturers, growers, consultants, extension services and grower organizations, working closely with regulators, to achieve logistically and economically feasible systems that prolong the effectiveness of all pest-control products.     

The impact of controlled release capsules of monensin on postcalving haptoglobin concentrations in dairy cattle

This study was designed to assess the impact of a controlled release capsule (CRC) of monensin, administered prior to calving, on postcalving haptoglobin levels. The role of disease on haptoglobin levels was also studied. The study population consisted of 1010 cows from 25 Holstein dairy herds near Guelph, Ontario. Monensin CRC or placebo capsules were randomly assigned within each herd 3 wk prior to the expected calving date. Serum from week 1 and week 6 postcalving was submitted for quantification of haptoglobin concentrations. Haptoglobin results were analyzed for associations with treatment, health data, and individual cow factors up to 95 d in milk. Haptoglobin concentrations were higher in week 1 than week 6 (P < 0.05). In univariate analysis, several diseases were significantly associated with haptoglobin concentrations. However, occurrence of disease appeared to be a confounding factor in the data interpretation. Thus, the analysis was stratified by the presence or absence of disease. There appeared to be associations between factors other than clinical disease contributing to increased haptoglobin levels in both clinically healthy and unhealthy cattle. Haptoglobin served as a good indicator of inflammatory disease. Monensin CRC treatment was associated with increased haptoglobin concentrations in clinically unhealthy cattle, perhaps reflecting a better ability to respond to disease challenge. The lower haptoglobin concentrations in monensin CRC treated cattle that were clinically normal may be a reflection of reduced subclinical disease.