小麦根腐离蠕孢菌诱导小麦β-1,3-葡聚糖酶基因的克隆及其表达

为了明确小麦受小麦根腐离蠕孢侵染后β-1,3-葡聚糖酶基因的表达情况,采用RT-PCR方法在受小麦根腐离蠕孢菌侵染后的小麦叶片中扩增β-1,3-葡聚糖酶基因,然后对侵染后的小麦叶片总RNA进行Northern斑点杂交。结果表明,克隆到了β-1,3-葡聚糖酶基因,该基因在小麦被侵染12 h时开始表达,在48 h后未见表达。对照中未见表达。可推断出此β-1,3-葡聚糖酶基因是被小麦根腐离蠕孢菌（Bipolaris sorokinianum）侵染后诱导表达的。     

西北干旱地区边际土地高抗逆纤维类能源植物的筛选与初探

合理利用边际土地,筛选种植高抗逆新型能源植物,为发展生物质能源提供足够的生物能源植物原料已成为解决目前能源危机的一个重要方向。本研究针对我国西北干旱区广泛分布的边际土地的优势,以甘肃省民勤县和榆中县为代表,对该地区高抗逆的纤维类植物进行了实地调查,分析这些调查植物的生长习性,并选取了分布广泛且高抗逆的甘肃省民勤县11种植物、榆中县9种植物,采集这些植物地上部分,测定能源指标含量,包括综纤维、木质素、灰分和热值,进一步利用科学合理的方法评价各个植物是否适宜作为能源植物,从而为西北干旱地区高抗逆新型纤维类能源植物的初步筛选和开发利用提供理论依据和技术支撑。结果表明,草本植物的综纤维含量高于木本植物,木质素含量低于木本植物,但灰分含量较高。综合生态适应性、化学成分和热值三项指标可得,草本植物芨芨草、冰草和旱生芦苇及木本植物柠条、野蔷薇、花棒可以作为潜在的高抗逆能源植物。柠条作为已公认的能源植物,目前已经大面积的种植和开发利用,这也为其他高抗逆能源植物的开发和利用提供重要的参考和借鉴。     

宿迁市林地利用现状及对策初探

宿迁市现有林地资源201089.36 hm2,活立木蓄积量15327818.51 m3,林业产业发达。但是,存在林地结构不合理、树种结构单一、中龄林比重过大和生态功能一般等问题。笔者从优化林业产业结构协调发展、转变模式分类推进、加强管护定向培育和科技支撑提高水平等方面提出了对策和建议。     

浅析学分制下高校课程设置的改革

我国高等学校传统的课程设置具有狭隘、封闭、开放性不强等弊端,已不能适应当前高等教育培养综合性高素质人才这一培养目标的要求.高校课程改革是高等教育在继实行学分制等全方位的体制改革和大范围的布局结构调整之后被提出来的.文中就高校课程改革应遵循的原则、改革的具体思路和举措等方面进行了探讨和分析.     

蝴蝶兰27个品种观赏性状评价

应用主成分分析法对蝴蝶兰27个品种的12个观赏性状进行分析.结果表明:12个性状指标可以分为6个主成分.主成分的方差贡献率达全部性状信息的86.61％；通过计算各品种的重要主成分值.得出10个观赏性状优良的品种.其结果与实际情况相近.     

多目标随机结盟对策的区间模糊ZS -值

内部控制和企业价值之间存在着密切的联系，内部控制是否有效及效率高低影响着企业的价值。内部控制质量高的企业有动机通过自愿性信息披露向市场传递企业价值较高的信号，由于信号传递作用，企业更容易获得较低成本的商业信用和银行信用借款，提升了企业价值，而内部控制质量低的企业没有动机进行内部控制信息披露，从而不得不付出较高的债务融资成本，降低了企业价值。     

Effects of ibrolipim against atherosclerosis in minipigs

AIM: To explore the application mechanism of NO-1886 (ibrolipim), a synthetic compound, improving dyslipidemia and inhibiting atherosclerosis in Guizhou minipigs fed with high fat/high sucrose diet. METHODS: Fifteen Chinese Guizhou minipigs were randomized into three groups with similar body weight ［(n=5 in normal control group (CD); n=5 in high fat/high sucrose group (HFSD); n=5 in high fat/high sucrose supplemented ibrolipim group （HFSD+ibrolipim)］. Blood samples were withdrawn from the eyehole sinus venosus of the animals at the end of each month after fasting overnight. The animals were sacrificed at the end of 8 months. The concentrations of cholesterol ester in plasma HDL were analyzed by HPLC. The aortic fatty streak-lesions were quantified following lipid staining with Sudan IV. Lipid droplets in liver were observed by Oil red O staining. RESULTS: Compared with CD, fasting plasma TC, TG and FFA levels of HFSD were elevated significantly. The aortic fatty streak-lesions were clearly presented in the animals’ aortas. The intima became rougher and thicker. A lot of lipoid foam cells migrated to regions of intima and smooth muscle cells, which associated with the injuries of internal elastic lamina. Extensive fat deposited in the liver were observed. Supplementing of 1.0% ibrolipim into high fat/high sucrose diet induced the decrease in plasma TG and FFA concentrations and an increase in plasma HDL-C concentration compared with HFSD. A little fat deposited in the liver were observed. CONCLUSION: ibrolipim prevents AS in high fat/high sucrose diet feeding minipigs through decreasing the plasma TG and elevating the plasma HDL-C.     

共轭亚油酸对动物免疫调节的研究

共轭亚油酸是一种功能性脂肪,具有抗癌、抗动脉硬化、抗糖尿病、抗氧化、降低脂肪沉积、促进骨生成、提高免疫力和缓和狼疮等功能。     

Identification of polymorphisms and association analysis with meat quality traits in the porcine KIAA1717 and HUMMLC2B genes

Skeletal muscle genes are potential candidates for production and meat quality. Screening a subtracted cDNA library constructed with mRNA obtained from longissimus dorsi muscles of F1 hybrids Landrace × Yorkshire and their female parents Yorkshire, we isolated two partial sequences coding for the H3-K4-specific methyltransferase (KIAA1717) and skeletal muscle myosin regulatory light chain (HUMMLC2B) genes. Database search revealed KIAA1717 and HUMMLC2B encoded proteins with SET domain and EF-hand calcium binding motif, respectively. In the present work we identified their partial polymorphisms and two SNPs, one (C1354T) at the 3′ untranslated region (UTR) of KIAA1717 and one (A345G) at the SINE (PRE-1) element of HUMMLC2B, both created/disrupted a restriction site for endonuclease Msp I. The selected pigs were genotyped at the KIAA1717 C1354T and HUMMLC2B A345G sites by means of a PCR-RFLP protocol. Significant associations were observed for the KIAA1717 C1354T polymorphic site with meat marbling (longissimus doris (p < 0.05), biceps femoris (p < 0.01)) and intramuscular fat (p < 0.01). HUMMLC2B A345G were significantly associated with meat pH (longissimus doris (p < 0.05), biceps femoris (p < 0.01)), drip loss (p < 0.01), water holding capacity (p < 0.01) and meat color value (longissimus doris (p < 0.01), biceps femoris (p < 0.05)). Further studies are needed to confirm these preliminary results.     

鹅细小病毒河北流行株HBZF07 NS1基因的克隆与序列分析

鹅细小病毒HBZF07株经13日龄鹅胚增殖后收集尿囊液,提取病毒基因组总DNA,采用PCR方法,一次性扩增出与预期大小相符的特异性条带.将扩增产物提纯回收后克隆入pMD18-T载体,经转化、筛选及酶切鉴定后,对阳性克隆进行了序列测定和序列分析.测序后拼接的基因长1 892 bp,包含完整的NS1开放阅读框(1 884 bp),编码623个氨基酸.与GenBank中其它毒株的NS1基因核苷酸序列相比,同源性分别为DY(EF515837)98.4%,与B(GPU25749)为99.2%,与HG5/82(AY506546)为93.9%,与YG(AF416726)为93.9%,与SHM319(GPU34761)为97.0%.