全文获取类型
收费全文 | 384篇 |
免费 | 29篇 |
专业分类
林业 | 22篇 |
农学 | 30篇 |
41篇 | |
综合类 | 33篇 |
农作物 | 54篇 |
水产渔业 | 56篇 |
畜牧兽医 | 149篇 |
园艺 | 3篇 |
植物保护 | 25篇 |
出版年
2023年 | 2篇 |
2022年 | 7篇 |
2021年 | 21篇 |
2020年 | 10篇 |
2019年 | 14篇 |
2018年 | 15篇 |
2017年 | 3篇 |
2016年 | 8篇 |
2015年 | 13篇 |
2014年 | 16篇 |
2013年 | 18篇 |
2012年 | 33篇 |
2011年 | 33篇 |
2010年 | 11篇 |
2009年 | 28篇 |
2008年 | 35篇 |
2007年 | 15篇 |
2006年 | 20篇 |
2005年 | 16篇 |
2004年 | 29篇 |
2003年 | 12篇 |
2002年 | 8篇 |
2001年 | 5篇 |
2000年 | 8篇 |
1999年 | 1篇 |
1998年 | 3篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 5篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1970年 | 2篇 |
1965年 | 1篇 |
1964年 | 4篇 |
1963年 | 3篇 |
排序方式: 共有413条查询结果,搜索用时 0 毫秒
91.
Dong Woo Chang Bora Kim Jae Hoon Shin Young Min Yun Jung Ho Je Yeu kuang Hwu Jung Hee Yoon Je Kyung Seong 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(2):107-113
The X-ray synchrotron is quite different from conventional radiation sources. This technique may expand the capabilities of conventional radiology and be applied in novel manners for special cases. To evaluate the usefulness of X-ray synchrotron radiation systems for real time observations, mouse fetal skeleton development was monitored with a high resolution X-ray synchrotron. A non-monochromatized X-ray synchrotron (white beam, 5C1 beamline) was employed to observe the skeleton of mice under anesthesia at embryonic day (E)12, E14, E15, and E18. At the same time, conventional radiography and mammography were used to compare with X-ray synchrotron. After synchrotron radiation, each mouse was sacrificed and stained with Alizarin red S and Alcian blue to observe bony structures. Synchrotron radiation enabled us to view the mouse fetal skeleton beginning at gestation. Synchrotron radiation systems facilitate real time observations of the fetal skeleton with greater accuracy and magnification compared to mammography and conventional radiography. Our results show that X-ray synchrotron systems can be used to observe the fine structures of internal organs at high magnification. 相似文献
92.
93.
Radioprotective effects of an acidic polysaccharide of Panax ginseng on bone marrow cells 总被引:1,自引:0,他引:1
Hyun-Ji Kim Mi-Hyoung Kim Yun-Young Byon Jae Woo Park Youngheun Jee Hong-Gu Joo 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(1):39-44
An acidic polysaccharide of Panax ginseng (APG), so called ginsan is known to have important immunomodulatory activities. It was recently reported that APG has radioprotective effects in mice but the detailed mechanism was not fully elucidated. This study examined the effects of APG on bone marrow cells (BMs). The phenotypical and functional changes in APG-treated BMs after gamma radiation were studied. The benefit of APG on BMs damaged by gamma radiation was determined by measuring the cell viability. Using 2 different assays, a pretreatment with APG significantly increased the viability of BMs against gamma radiation. APG-treated BMs had a significantly higher amount of IL-12, which is a major cytokine for immune responses, compared with the medium-treated BMs. The expression of MHC class II molecules of APG-treated BMs was also increased, and APG-treated BMs showed significantly higher levels of allogeneic CD4+ T lymphocyte proliferation. Furthermore, APG-treated mice had a larger number of BMs after gamma radiation than the control mice, and the BMs of APG-treated mice were successfully cultured into dendritic cells, which are the representative antigen-presenting cells. Overall, this study shows that APG alters the phenotype of BMs, increases the viability and alloreactivity of BMs after gamma radiation both in vitro and in vivo. Therefore, APG may be a good candidate radioprotective agent for BMs. 相似文献
94.
Little is known of the association of enterohemorrhagic Escherichia coli O157:H7/NM (EHEC O157) with disease in naturally infected calves, although cattle have been known as a major source for EHEC O157 outbreaks in humans. In this study, we investigated the occurrence of EHEC O157 in calves associated with/without diarrhoea to examine if EHEC O157 is involved in calf diarrhoea and to characterize the isolates. Four hundred and ninety eight diarrhoeic and non-diarrhoeic young calves from 115 different farms were examined. Of 244 diarrhoeic calves, 24 (9.8%) were positive for EHEC O157, and of 254 non-diarrhoeic calves, 7 (2.8%) were positive. EHEC O157 was recovered from 12/76 (15.79%) of diarrhoeic calves less than 2-week-old, and no EHEC O157 was detected in this age group of non-diarrhoeic calves. This implicates EHEC O157 as a possible cause of the disease in naturally infected neonatal calves. The occurrence of EHEC O157 was relatively lower in the older calves (aged older than 8 weeks) and no significant difference was found in the occurrence rates between these diarrhoeic and non-diarrhoeic calves. PCR analysis of virulence markers revealed that the isolates carried various virulence genes such as Ehly, eae, stx1 and stx2, which underlines the potential importance of these attributes for the infection, colonization and possible pathogenesis of calf diarrhoea. 相似文献
95.
Seonghyu Shin Seong-Guk Kim Gun-Ho Jung Chung-Guk Kim Beom-Young Son Jeong Tae Kim Sang Gon Kim Woonho Yang Youngup Kwon Kang-Bo Shim Mi-Ok Woo 《Journal of Crop Science and Biotechnology》2016,19(5):393-399
Maize is highly susceptible to waterlogging, which is becoming one of worldwide abiotic threats in many agricultural areas. This study was evaluated to establish the screening method and to find tolerant maize genotypes. Six Korean maize inbred lines were subjected to waterlogging at V3 for 15 days using a big size pot with single maize plant (big pot method) and a box containing 31 maize plants at a time (box method). The degree of foliar senescence and the number of senescent leaves were better indicators for selecting waterlogging tolerant maize genotypes than SPAD value and plant height. The degree of foliar senescence revealed that KS124, KS140, and KS141 are tolerant, and KS85 is susceptible to waterlogging at the early growth stage. These responses of foliar senescence were in clear accordance with those of plant grain yield, which was supported by stress tolerance index for grain yield. The box method also showed the similar response of foliar senescence to the big pot method. Therefore, this box method based on foliar senescence may be simple and efficient for large-scale screening of maize germplasm against waterlogging stress. It was concluded that foliar senescence can be a good indicator for selecting tolerant maize genotypes against waterlogging at the early growth stage. 相似文献
96.
Kota Tsutsui Bum Hee Jeong Yukiko Ito Sang Woo Bang Yukio Kaneko 《Breeding Science》2011,61(4):373-379
Intergeneric hybridization was performed between Moricandia arvensis and four inbred lines of Brassica rapa following embryo rescue. Three F1 hybrid plants were developed from three cross combinations of M. arvensis × B. rapa, and amphidiploids were synthesized by colchicine treatment. Six BC1 plants were generated from a single cross combination of amphidipolid × B. rapa ‘Ko1-303’ through embryo rescue. One BC2 and three BC3 plants were obtained from successive backcrossing with B. rapa ‘Ko1-303’ employing embryo rescue. Alloplasmic and monosomic addition lines of B. rapa (Allo-MALs, 2n = 21) were obtained from backcrossed progeny of three BC3 plants (2n = 21, 22 and 23) without embryo rescue. An alloplasmic line of B. rapa (2n = 20) degenerated before floliation on 1/2 MS medium due to severe chlorosis. Allo-MALs of B. rapa (2n = 21) showed stable male sterility without any abnormal traits in vegetative growth and female fertility. Molecular analyses revealed that the same chromosome and cytoplasm of M. arvensis had been added to each Allo-MAL of B. rapa. This Allo-MAL of B. rapa may be useful material for producing cytoplasmic male sterile lines of B. rapa. 相似文献
97.
Hyoung Seok Lee Munusamy Madhaiyan Chung Woo Kim Seung Ju Choi Keun Yook Chung Tong Min Sa 《Biology and Fertility of Soils》2006,42(5):402-408
Three plant-growth promoting, N2-fixing methylotrophic strains isolated from rice cultivars (Oryza sativa L.), viz, Methylobacterium sp. CBMB20, Enterobacter sp. CBMB30, Burkholderia sp. CBMB40, were selected, and their activities in promoting the early growth of rice were studied. Seeds treated with the methylotrophic strains improved seed germination, seedling vigor index (SVI), and biomass of rice seedlings. The methylotrophic population in the treated seedlings increased in the vegetative stages when compared to seeding stages. Treated seedlings showed a higher accumulation of plant hormones viz trans-zeatin riboside, isopentenyladenosine, and indole-3-acetic acid than untreated seedlings. Plant hormones were detected immunologically using the phytodetek kit. Conformational evidence suggested that cytokinins were produced by the epiphytic bacteria colonizing the plants rather than by the plants themselves. In addition, the inoculated early stage rice seedlings also exhibited a wide range of acetylene reduction activity. The results suggest the potential use of these bacteria to stimulate germination, SVI, and biomass production, which is mediated by production of plant hormone accumulation and nitrogen fixation. 相似文献
98.
Seventy‐two pigs at 34.4 kg body weight (BW) were allotted to two treatments with six replicates/treatment and six pigs/pen: the CON (negative control, no added selenium (Se)) and the OS (0.36 mg/kg added selenium from selenium‐enriched yeast). Pigs were fed until 130 kg BW. The CON diet contained 0.18 mg/kg indigenous Se whereas the OS diet contained 0.54 mg/kg Se. Blood samples were collected at 130 kg BW and further processed for microarray analysis, prepared with 885 genes related to immune function of pigs. Among those, 28 genes related to improved immune status and innate immunity were up‐regulated (P < 0.05) in leukocytes from Se‐fed pigs and those include major histocompatibility class I (> 1.66), arginase I (> 1.27), integrin beta‐1‐subunit (> 1.20), toll like receptor 2 (> 1.12) and double‐stranded RNA‐dependent protein kinase. However, 24 genes including tissue factor (< 4.70), serum amyloid A‐2 protein (< 3.11) and p27Kip1 (< 1.42) were down‐regulated (P < 0.05) in leukocytes from Se‐fed pigs. Expression of four selected genes was validated using quantitative PCR (qPCR) showing significant correlation between mircroarray analysis and qPCR analysis. This study indicates that a long‐ term dietary supplementation (0.3%) of organic Se improves the expression of genes that are related to enhanced immunity of pigs. 相似文献
99.
用800个中国水稻品种试验的结果指出:不同地区原产的品种不但对光照长度的反应特性不同,而且在短日照下的生育期也不同。这种特性称为短日生育性。北方的品种短日生育性较小;南方的品种从小到大都有。品种成熟期的早晚是其光照长度反应特性和短日生育性在一定环境条件下的具体表现,改变环境条件时,早晚熟的顺序随之改变。对光照长度反应弱的品种一般产量变化小,适应力大。试验结果得出各地区品种的光长反应特性和短日生育性。 相似文献
100.
Kwon Soo-Jeong Roy Swapan Kumar Yu Jang-Hwan Cho Seong-Woo Kim Hag-Hyun Boo Hee-Ock Woo Sun-Hee 《Journal of Crop Science and Biotechnology》2019,22(5):465-474
Journal of Crop Science and Biotechnology - The root of Platycodon grandiflorum is used as traditional oriental medicine in Asian countries since ancient times to treat bronchitis, tonsillitis,... 相似文献