全文获取类型
收费全文 | 1574篇 |
免费 | 114篇 |
国内免费 | 35篇 |
专业分类
林业 | 110篇 |
农学 | 133篇 |
基础科学 | 36篇 |
258篇 | |
综合类 | 214篇 |
农作物 | 193篇 |
水产渔业 | 112篇 |
畜牧兽医 | 558篇 |
园艺 | 21篇 |
植物保护 | 88篇 |
出版年
2024年 | 2篇 |
2023年 | 10篇 |
2022年 | 34篇 |
2021年 | 58篇 |
2020年 | 45篇 |
2019年 | 63篇 |
2018年 | 43篇 |
2017年 | 59篇 |
2016年 | 50篇 |
2015年 | 83篇 |
2014年 | 87篇 |
2013年 | 97篇 |
2012年 | 108篇 |
2011年 | 114篇 |
2010年 | 76篇 |
2009年 | 72篇 |
2008年 | 115篇 |
2007年 | 101篇 |
2006年 | 79篇 |
2005年 | 73篇 |
2004年 | 59篇 |
2003年 | 54篇 |
2002年 | 49篇 |
2001年 | 36篇 |
2000年 | 32篇 |
1999年 | 23篇 |
1998年 | 11篇 |
1997年 | 12篇 |
1996年 | 12篇 |
1995年 | 5篇 |
1994年 | 5篇 |
1993年 | 8篇 |
1992年 | 9篇 |
1991年 | 2篇 |
1990年 | 7篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1985年 | 4篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1974年 | 2篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1966年 | 1篇 |
1965年 | 1篇 |
1955年 | 1篇 |
1950年 | 1篇 |
1949年 | 1篇 |
1908年 | 1篇 |
排序方式: 共有1723条查询结果,搜索用时 15 毫秒
131.
Cha KH Lee JY Song DG Kim SM Lee DU Jeon JY Pan CH 《Journal of agricultural and food chemistry》2011,59(16):8670-8674
Chlorella is a nutrient-rich microalga that contains protein, lipid, minerals, vitamins, and high levels of lutein. This study evaluated the bioavailability of lutein from Chlorella vulgaris using a coupled in vitro digestion and human intestinal Caco-2 cell model. Lutein bioaccessibility was low, and approximately 75% of total C. vulgaris lutein was not micellized during the digestion process but remained in the insoluble digestate. Microfluidization improved lutein micellization efficiency during C. vulgaris digestion. C. vulgaris was microfluidized at a pressure exceeding 10000 psi, and the cell surface disruption was visualized by scanning electron microscopy. The mean C. vulgaris particle size was reduced from 3.56 to 0.35 μm with the microfluidization treatment. C. vulgaris microfluidization at 20000 psi was three times more efficient for aqueous lutein micelles production as compared with untreated C. vulgaris, and the final lutein content accumulated by intestinal Caco-2 cells was also higher with microfluidization. C. vulgaris lutein stability was not affected by microfluidization. These results indicate that microfluidization may be useful for improving lutein bioaccessibility from C. vulgaris during food processing. 相似文献
132.
Shin C Hyun J Lim Y Kim JS Jang YM Kang SJ 《Journal of agricultural and food chemistry》2011,59(21):11579-11584
In this study, a derivative of p-phenoxybenzaldehyde in bamboo shoots was investigated. Bamboo shoots were ground and extracted with water, and an aqueous suspension was purified by SPE using Oasis HLB cartridges. After the SPE procedure, the analytes were analyzed by HPLC with refractive index detection (HPLC-RI). In the HPLC-RI analysis for sucralose, a putative sucralose was detected. In the subsequent HPLC-PDA analysis, the suspicious peak showed a unique UV absorption spectrum with the maximum wavelength at 285 nm indicating the existence of an aromatic ring. The contents of the unknown compound in bamboo shoot products ranged from 0.01 to 0.15 mg/g. The identity of the unknown compound was further confirmed by HPLC-ESI/MS/MS. The molecular weight of the unknown compound was determined to be 244. The chemical structure of the unknown compound was elucidated on the basis of NMR spectroscopic analyses ((1)H, (13)C, DEPT, COSY, HMQC, and HMBC). Finally, the structure of the unknown compound was characterized as 4-(4-dihydroxymethylphenoxy)benzaldehyde. 相似文献
133.
Arash Minai-Tehrani Young-Chan Park Soon-Kyung Hwang Jung-Taek Kwon Seung-Hee Chang Sung-Jin Park Kyeong-Nam Yu Ji-Eun Kim Ji-Young Shin Ji-Hye Kim Bitna Kang Seong-Ho Hong Myung-Haing Cho 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(4):309-317
Conventional lung cancer therapies are associated with poor survival rates; therefore, new approaches such as gene therapy are required for treating cancer. Gene therapies for treating lung cancer patients can involve several approaches. Among these, aerosol gene delivery is a potentially more effective approach. In this study, Akt1 kinase-deficient (KD) and wild-type (WT) Akt1 were delivered to the lungs of CMV-LucR-cMyc-IRES-LucF dual reporter mice through a nose only inhalation system using glucosylated polyethylenimine and naphthalene was administrated to the mice via intraperitoneal injection. Aerosol delivery of Akt1 WT and naphthalene treatment increased protein levels of downstream substrates of Akt signaling pathway while aerosol delivery of Akt1 KD did not. Our results showed that naphthalene affected extracellular signal-regulated kinase (ERK) protein levels, ERK-related signaling, and induced Clara cell injury. However, Clara cell injury induced by naphthalene was considerably attenuated in mice exposed to Akt1 KD. Furthermore, a dual luciferase activity assay showed that aerosol delivery of Akt1 WT and naphthalene treatment enhanced cap-dependent protein translation, while reduced cap-dependent protein translation was observed after delivering Akt1 KD. These studies demonstrated that our aerosol delivery is compatible for in vivo gene delivery. 相似文献
134.
Ji Hyung Kim Sun Young Hwang Jee Soo Son Jee Eun Han Jin Woo Jun Sang Phil Shin Casiano Choresca Jr Yun Jaie Choi Yong Ho Park Se Chang Park 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(1):41-48
The antibiotic resistance of 16 Aeromonas (A.) salmonicida strains isolated from diseased fish and environmental samples in Korea from 2006 to 2009 were investigated in this study. Tetracycline or quinolone resistance was observed in eight and 16 of the isolates, respectively, based on the measured minimal inhibitory concentrations. Among the tetracycline-resistant strains, seven of the isolates harbored tetA gene and one isolate harbored tetE gene. Additionally, quinolone-resistance determining regions (QRDRs) consisting of the gyrA and parC genes were amplified and sequenced. Among the quinolone-resistant A. salmonicida strains, 15 harbored point mutations in the gyrA codon 83 which were responsible for the corresponding amino acid substitutions of Ser83→Arg83 or Ser83→Asn83. We detected no point mutations in other QRDRs, such as gyrA codons 87 and 92, and parC codons 80 and 84. Genetic similarity was assessed via pulsed-field gel electrophoresis, and the results indicated high clonality among the Korean antibiotic-resistant strains of A. salmonicida. 相似文献
135.
Miyoung Yang Myoung-Sub Song Sung-Ho Kim Jong-Choon Kim Joong-Sun Kim Taekyun Shin Changjong Moon 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(3):203-207
This in vitro study evaluated the detrimental effect of acute gamma (γ)-irradiation on rat immature hippocampal neurons. Rat immature hippocampal neurons (0.5 day in vitro) were irradiated with 0~4 Gy γ-rays. Cytotoxicity was analyzed using a lactate dehydrogenase release assay at 24 h after γ-irradiation. Radiation-induced cytotoxicity in immature hippocampal neurons increased in a dose-dependent manner. Pre-treatments of pro-apoptotic caspase inhibitors and anti-oxidative substances significantly blocked γ-irradiation-induced cytotoxicity in immature hippocampal neurons. The results suggest that the caspase-dependent cytotoxicity of γ-rays in immature hippocampal cultured neurons may be caused by oxidative stress. 相似文献
136.
Min‐Jung GU Md. Jahangir ALAM Seon‐Ho KIM Che‐Ok JEON Moon‐Baek CHANG Young‐Kyoon OH Sang‐Cheol LEE Sang‐Suk LEE 《Animal Science Journal》2011,82(5):663-672
Molecular diversity of methanogens in the rumen of Korean black goats was investigated with 16S rRNA gene clone libraries using methanogen‐specific primers. The libraries were composed of rumen fluid‐associated methanogens (FAM) and rumen particle‐associated methanogens (PAM) from rumen‐fistulated Korean black goats. Among the 141 clones of the FAM library, the sequences were mostly related to two phyla, the Methanobacteriaceae family (77.3%) and the Thermoplasmatales family (22.7%); and among the 68 clones of the PAM library, sequences were also mainly clustered in the two phyla, the Thermoplasmatales family (63.24%) and the Methanobacteriaceae family (35.29%). Most of the sequenced clones in the two libraries were closely related to uncultured methanogenic archaeon. Quantitative real‐time PCR revealed that PAM (8.97 log 10) had significantly higher (P < 0.01) density of methanogens by the methanogenic 16S rRNA gene copies than FAM (7.57 log 10). The two clone libraries also showed difference in Shannon index (FAM library 1.70 and PAM library 1.59) and Chao 1 estimator (FAM library 18 and PAM library 17 operational taxonomic units). Apparent differences found in the microbial community from the two 16S rRNA gene libraries could be a result of such factors as the chemical and physical nature of the target material surface, types or component of diets, the interaction between the methanogens and other microbes, and age of the experimental goats. 相似文献
137.
剖析警犬侦破命案的成功案例,笔者发现带犬民警在命案现场的勘查工作至关重要。现场勘查工作仔细、有成效,警犬技术就能快速发挥作用;反之,警犬技术则无 相似文献
138.
139.
Flavonol glycosides and novel iridoid glycoside from the leaves of Morinda citrifolia 总被引:5,自引:0,他引:5
Sang S Cheng X Zhu N Stark RE Badmaev V Ghai G Rosen RT Ho CT 《Journal of agricultural and food chemistry》2001,49(9):4478-4481
One new iridoid glycoside and five known flavonol glycosides have been isolated from the leaves of Morinda citrifolia. The new iridoid exists as an epimeric mixture in solution. Complete assignments of the proton and carbon chemical shifts for the individual epimers were accomplished on the basis of high-resolution 1D and 2D NMR data. Their antioxidative activities were measured. All of these compounds showed DPPH free radical scavenging activity at the concentration of 30 microM. 相似文献
140.
Sato D Awad AA Chae SH Yokota T Sugimoto Y Takeuchi Y Yoneyama K 《Journal of agricultural and food chemistry》2003,51(5):1162-1168
A simple and rapid analytical method for strigolactones, germination stimulants for the root parasitic weeds witchweed (Striga spp.) and broomrape (Orobanche spp.), has been developed using high-performance liquid chromatography connected to tandem mass spectrometry (LC/MS/MS). The natural strigolactones (strigol, sorgolactone, orobanchol, and alectrol) were clearly separated and identified by LC/MS/MS. As low as 0.1 pg/microL of strigol and 0.5 pg/microL of sorgolactone could be quantified, whereas 1 pg/microL was needed for the quantification of orobanchol (S/N > 10). Using this method, it was found that red clover produces orobanchol and alectrol but not strigol. The roots of red clover seedlings were found to produce 13, 70, 58, and 65 pg of orobanchol/plant 1, 2, 3, and 4 weeks after germination, respectively. 相似文献