全文获取类型
收费全文 | 505篇 |
免费 | 67篇 |
专业分类
林业 | 26篇 |
农学 | 16篇 |
基础科学 | 5篇 |
53篇 | |
综合类 | 42篇 |
农作物 | 20篇 |
水产渔业 | 44篇 |
畜牧兽医 | 337篇 |
园艺 | 5篇 |
植物保护 | 24篇 |
出版年
2023年 | 3篇 |
2022年 | 9篇 |
2021年 | 11篇 |
2020年 | 17篇 |
2019年 | 19篇 |
2018年 | 16篇 |
2017年 | 29篇 |
2016年 | 20篇 |
2015年 | 17篇 |
2014年 | 20篇 |
2013年 | 32篇 |
2012年 | 13篇 |
2011年 | 25篇 |
2010年 | 22篇 |
2009年 | 19篇 |
2008年 | 27篇 |
2007年 | 27篇 |
2006年 | 31篇 |
2005年 | 31篇 |
2004年 | 16篇 |
2003年 | 17篇 |
2002年 | 17篇 |
2001年 | 12篇 |
2000年 | 6篇 |
1999年 | 15篇 |
1998年 | 12篇 |
1997年 | 15篇 |
1996年 | 10篇 |
1995年 | 8篇 |
1994年 | 5篇 |
1993年 | 5篇 |
1992年 | 9篇 |
1991年 | 8篇 |
1990年 | 3篇 |
1989年 | 5篇 |
1988年 | 2篇 |
1987年 | 3篇 |
1986年 | 4篇 |
1985年 | 1篇 |
1982年 | 2篇 |
1978年 | 1篇 |
1975年 | 1篇 |
1974年 | 2篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1966年 | 3篇 |
排序方式: 共有572条查询结果,搜索用时 230 毫秒
21.
Syndromic surveillance systems can enhance early disease warning, endemic disease monitoring, or help to accumulate proof of disease freedom. In order to provide immediate feedback to achieve these goals, the health data sources scanned should be acquired continuously, in an automated fashion, and should be stored electronically. Recognizing that data from diagnostic test requests often meet these requirements, two systems designed to automatically extract surveillance information from animal laboratory databases have been developed and are described in this paper. These systems are designed to contribute to early disease detection, as well as the timely management of epidemiological information, in a province of Canada and in Sweden, the areas served by the diagnostic laboratories concerned. Classifying in-coming requests into syndromes, the first step, was the most time-consuming and the least portable step between the two systems. The remaining steps were more easily adjusted from one system to implementation in the other. These steps included: retrospective evaluation of data to create baseline profiles following the removal of excessive noise and aberrations; the identification of temporal effects; prospective evaluation of detection algorithms; and finally real-time monitoring and implementation. Building upon the institutions’ existing data management software, all steps to use those data for the purposes of syndromic surveillance were set up using open source software; as a result this approach could be readily adopted by other institutions. Relatively straight-forward development and maintenance is expected to lead to the incorporation of these systems into each institution's surveillance processes, becoming an indispensable tool for diagnosticians and epidemiologists, as well as stimulating further technical development of such systems. 相似文献
22.
23.
Anne-Sophie Sergent Nathalie Bréda Léopoldo Sanchez Jean-Charles Bastein Philippe Rozenberg 《Annals of Forest Science》2014,71(6):709-720
Context
Since the 2003 drought and heat wave, Douglas-fir dieback has been reported in France in trees older than 30 years. Consequently, it is questioned whether selected Douglas-fir provenances are suited to the frequent and severe drought events which are forecast due to climate change.Aims
Our objective was to contribute to the screening of variability in productivity and growth response to soil-water deficit of mature trees from provenances not currently used for plantation in France.Methods
We sampled 22 provenances, including coastal and interior Douglas-fir, covering a wide part of its natural distribution, from Oregon to California for coastal provenances and from British Columbia to New Mexico for interior provenances. These provenances were planted at the mid 1970s in two provenance trials located in the south-west area of France. Variability of productivity, of wood density, and of radial growth in response to drought episodes among provenances was quantified and related to soil-water deficit computed by daily water balance calculations.Results
Whatever the provenance, annual radial growth is highly dependent on local soil-water deficit (Felines R 2 = 0.57, Le Treps R2 = 0.49). Radial growth and wood properties exhibit large differences between provenances at 30 years old. Variability between provenances for all wood characteristics studied is mainly structured geographically. Coastal provenances perform best for productivity at 30 years old (619 cm2?±?59), and exhibit a small growth reduction in 2004, the second successive year of drought (?10.7 %?±?3.8). Surprisingly, the southern interior provenances from the driest environments in the natural range show a large growth reduction in 2004 (?30.5 %?±?5.2).Conclusions
The provenances tested exhibited significant differences in growth performance and growth reduction induced by the soil-water deficit. The approach coupling retrospective analysis of radial growth on mature trees and water balance modelling is relevant for evaluating provenance adaptation to more frequent or severe drought episodes. 相似文献24.
Effect of pH and Decapsulation on the Toxicity of Ammonia to the Brine Shrimp Artemia franciscana 总被引:1,自引:0,他引:1
Artemia franciscana were hatched and tested for tolerance to ammonia at pH 6.5 and 8.5 in artificial seawater with a salinity of 17 ‰. Nauplii seemed to be less tolerant of ammonia at pH 6.5 despite the fact that more of the ammonia is in the ionized form (NH4 + ). It is suggested that this greater sensitivity is a result of the NH4 + competing with the Na+ during gut transfer. Nauplii hatched from decapulated cysts were more tolerant of ammonia than those hatched from whole cysts, probably because of a greater energy reserve. 相似文献
25.
John M David Sandra Duarte Vogel Kimberly Longo Daniel Sanchez Gregory Lawson 《Veterinary anaesthesia and analgesia》2014,41(6):654-659
ObjectiveTo investigate a topical local anesthesia technique as a means to prevent and/or diminish pain in mice in a laboratory setting associated with tail vein injections performed by personnel in training.Study designProspective, randomized experimental trial.AnimalsThirty six adult female, 23–28 g CD-1 mice from an in-house training colony. They were acclimated to routine training and handling classes.MethodsEutectic mixture of local anesthetics (EMLA) cream (2.5% lidocaine/2.5% prilocaine) or a bland ointment control (n = 18) was applied on the tail prior to intravenous injection. The injections were performed by novices, who had never attempted the procedure, and experienced personnel. All participants were blinded to treatment groups. Three injection attempts were allowed per animal. The mice were observed and scored by blinded evaluators for behavioral and physiological changes, including respiratory rate, vocalization, tail flick, and escape behaviors, during and after the injection.ResultsThis study demonstrates that aversive behaviors induced by lateral tail vein injection were not changed by the preemptive application of EMLA cream. The aversive behaviors associated with lateral tail vein injection were significantly affected by the number of injection attempts and the individual's experience level.Conclusions and clinical relevanceTopical EMLA cream did not reduce signs of aversive reaction to tail vein injection and thus we did not find support for its use in mouse training programs for tail vein injections. 相似文献
26.
27.
C. SABATON L. SIEGLER V. GOURAUD J.L. BAGLINIÈRE & S. MANNÉ 《Fisheries Management and Ecology》1997,4(6):425-438
A mathematical model representing the long-term change in a trout population under different river management scenarios is presented. It describes the structure of a population broken down into age classes based on the Leslie matrix; if the population structure for any given month is known, the model should be able to estimate that of the following month. The passage from one month to the next takes into account various relevant factors: survival rate of individuals in the different age classes; fertility rate of females; linear and weighted growth rates; displacement linked to habitat fluctuations using weighted usable area (WUA) values. The model was applied to two French rivers. Regular monitoring of trout populations on the River Kernec enabled comparison of the response of the model with no displacement, with actual variations in fish stocks on the first river. In addition, the knowledge of WUA chronologies on the River Echez made it possible to carry out initial simulations of the response of a fish population to different river management scenarios at the second site. 相似文献
28.
S. Zia G. Romano W. Spreer C. Sanchez J. Cairns J. L. Araus J. Müller 《Journal of Agronomy and Crop Science》2013,199(2):75-84
The main task of this research was to evaluate canopy temperature and Crop Water Stress Index (CWSI) by assessing genotype variability of maize performance for different water regimes. To that end, three hundred tropical and subtropical maize hybrids with different phenology in terms of date of anthesis were evaluated. The influence of phenology on the change in canopy temperatures and CWSI was not equal during the three dates of measurement. At the end of vegetative growth (82 days after sowing, DAS) and at the blister stage (DAS 97), a high significant difference in temperatures and CWSI (P < 0.001) were obtained between the early‐ and late‐maturity genotypes. During anthesis (DAS 89), phenology had a significant effect (P < 0.01) only for the well‐watered genotypes, while under water‐stress conditions, no differences were found between early and late genotypes in terms of canopy temperature and CWSI. High significant differences (P < 0.001) in stomatal conductance (gs) between early and late genotypes for different treatments were observed. A relationship (R2 = 0.62) between gs and canopy temperature was obtained. Under a water‐stress canopy, temperature was measured at anthesis, which was negatively correlated with grain yield of the early (r = ?0.55)‐ and late (r = ?0.46)‐maturity genotypes in the water‐stressed condition. 相似文献
29.
Objective To determine the proportion and incidence of calves persistently infected with bovine pestivirus in calves (n = 1521) supplied to the Tick Fever Research Centre and to assess the test regime to detect calves persistently infected with bovine pestivirus.
Design Calves, 1 to 6 weeks old, selected for use in the production of the tick fever vaccine were collected from 21 properties in 56 separate groups between October 1990 and December 1996. Each group was examined for the presence of calves persistently infected with bovine pestivirus.
Procedure All calves were routinely tested for antibody to bovine pestivirus and bovine pestivirus antigen using a serum neutralisation test and an antigen-capture ELISA, respectively. Pooled lymphocyte samples from calves were also monitored for bovine pestivirus by inoculation of sheep. Whole herd testing was carried out in eight herds, using a serum neutralisation test as a screen test followed by an antigen-capture ELISA of cattle with a serum neutralisation test titre of less than 32.
Results Fourteen of the 1521 calves tested (0.9%), were detected as persistently infected and the incidence ranged from 0.0 to 3.0 % per year over 6 years. Persistently infected calves were found in 13 of the 59 groups and originated from 7 of the 21 herds used. In whole herd testing on the properties of origin, cattle persistently infected with bovine pestivirus were detected in four of the eight herds tested
Conclusions The proportion of calves persistently infected with bovine pestivirus is similar to that in other countries and indicates that bovine pestivirus could be a significant cause of economic loss in Australian cattle herds. In detecting calves persistently infected with bovine pestivirus, the combination of sheep inoculation, paired antigen-capture ELISA and serum neutralisation tests appeared to be highly sensitive and specific. 相似文献
Design Calves, 1 to 6 weeks old, selected for use in the production of the tick fever vaccine were collected from 21 properties in 56 separate groups between October 1990 and December 1996. Each group was examined for the presence of calves persistently infected with bovine pestivirus.
Procedure All calves were routinely tested for antibody to bovine pestivirus and bovine pestivirus antigen using a serum neutralisation test and an antigen-capture ELISA, respectively. Pooled lymphocyte samples from calves were also monitored for bovine pestivirus by inoculation of sheep. Whole herd testing was carried out in eight herds, using a serum neutralisation test as a screen test followed by an antigen-capture ELISA of cattle with a serum neutralisation test titre of less than 32.
Results Fourteen of the 1521 calves tested (0.9%), were detected as persistently infected and the incidence ranged from 0.0 to 3.0 % per year over 6 years. Persistently infected calves were found in 13 of the 59 groups and originated from 7 of the 21 herds used. In whole herd testing on the properties of origin, cattle persistently infected with bovine pestivirus were detected in four of the eight herds tested
Conclusions The proportion of calves persistently infected with bovine pestivirus is similar to that in other countries and indicates that bovine pestivirus could be a significant cause of economic loss in Australian cattle herds. In detecting calves persistently infected with bovine pestivirus, the combination of sheep inoculation, paired antigen-capture ELISA and serum neutralisation tests appeared to be highly sensitive and specific. 相似文献
30.
Sanchez CA Krieger RI Khandaker N Moore RC Holts KC Neidel LL 《Journal of agricultural and food chemistry》2005,53(13):5479-5486
The Colorado River is contaminated with perchlorate concentrations of 1.5-8 microg/L, an anion linked to thyroid dysfunction. Over 90% of the lettuce (Lactuca sativa L.) consumed during the winter months in the United States is produced in the Lower Colorado River region. Studies were conducted in this region to survey the potential for lettuce perchlorate accumulation and estimate potential human exposure to perchlorate from lettuce. Total uptake of perchlorate in the above-ground plant of iceberg lettuce was approximately 5 g/ha. Exposure estimates ranged from 0.45 to 1.8 microg/day depending on lettuce types and trimming. For all lettuce types, hypothetical exposures were less than 4% of the reference dose recommended by the National Academy of Sciences. Results show the relative iodide uptake inhibition potential because of lettuce nitrate was 2 orders of magnitude greater than that associated with the corresponding trace levels of perchlorate. These data support the conclusion that potential perchlorate exposures from lettuce irrigated with Colorado River water are negligible relative to acute or long-term harmful amounts. 相似文献