Assessing in vivo Fertilizing Capacity of Liquid-Preserved Boar Semen According to the 'Hanover Gilt Model'

The goal of this study was to determine the ability of the Hanover gilt model to assess in vivo fertilizing capacity of preserved sperm and to consider whether any modifications to this model were needed. This model evaluates the fertilizing capacity of semen based on the fertilization rate, the rate of normal embryos and the accessory sperm count of 3–5‐day embryos. Its distinguishing characteristics are the use of one‐time insemination of sperm in reduced numbers, of spontaneously ovulating gilts and of ovulation detection through ultrasound examination of ovaries. Reduced sperm numbers allow for an accurate evaluation of the fertilizing potential of different semen treatments, thereby avoiding the compensatory effect of doses calibrated to maximize fertility. The model's usefulness was assessed in a trial run designed to compare the fertilizing capacity of liquid boar semen diluted into two different extenders. The diluent, the boar and the backflow, had no significant effect on any of the parameters studied. Gilts inseminated less than 24 h before ovulation had a significantly higher (p < 0.01) fertilization rate and accessory sperm cell count (p < 0.05) than those inseminated more than 24 h before ovulation. Very good/good embryos from homogeneous litters (only very good/good embryos were present) had a significantly higher (p < 0.01) accessory sperm count than those from heterogeneous litters (at least one embryo was of a different quality and/or oocytes were present). Both very good/good and degenerated/retarded embryos from heterogeneous litters had low accessory sperm numbers. This suggests that accessory sperm count is significantly related to the quality of the litter, but not to the quality of the embryo within gilts. It can be concluded that the Hanover gilt model is sensitive enough to show fertility differences (in this study, those associated with in vivo ageing of semen), while using relatively few gilts and little time.     

2-anilino-3-methylbutyrates and 2-(isoindolin-2-yl)-3-methylbutyrates,two novel groups of synthetic pyrethroid esters not containing a cyclopropane ring

A new series of substituted 2-anilino-3-methylbutyrates has been prepared; bioassay data for these compounds on Heliothis virescens, Musca domestica, Aphis fabae and Tetranychus urticae are presented and discussed. Some unexpected relationships were observed between the nature of the substituents and the biological activity. Increases in foliar stability were noted with certain substitution patterns. Both α-cyano-3-phenoxybenzyl 3-methyl-2-(α, α, α,2-tetrafluoro-p-toluidino)butyrate and the corresponding 2-(2-chloro-α, α, α-trifluoro-p-toluidino)-3-methylbutyrate showed good stability in air and light, and exhibited biological activities of a similar nature and potency to those of previously known synthetic pyrethroids. Esters of the (R)-2- anilino-3-methylbutyric acids are far more active than those prepared from the (S)-enantiomers. The (R)-configuration at C-2 in these acids is sterically equivalent to the active absolute configuration at the chiral carbon α to the carboxylate group in both the permethrin and the fenvalerate types of pyrethroids. A new class of insecticidal 2-(isoindolin-2-yl)alkanoates is also reported. In this series the most biologically active analogue was α-cyano-3-phenoxybenzyl 3-methyl-2-(4,5,6,7-tetrafluoroisoindolin-2-yl)butyrate. These esters were considerably less stable than the anilino analogues on exposure to air and light.     

NONHUMAN PRIMATE EXCRETORY UROGRAPHY*

Survey radiographs have not accurately identified renal contour or location in nonhuman primates. Excretory urography using 786 to 1193 mg iodinelkg body weight resulted in dense opacification of the renal parenchyma and pelvis. Abdominal compression improved visu- alization of the renal pelvis. Interpretation of the nonhuman primate excretory urogram was compromised by several anatomic characteristics of this animal group. Superimposition of the kidneys on the lateral radiograph limited evaluation of the renal contour. Since the renal pelvis in most species of nonhuman primates does not possess diverticula (as in the dog and cat) or a caliceal system (as in the pig and man), the diagnosis of pyelonephritis or renal mass lesions was difficult. Severe irregularities in the renal contour, size of the renal pelvis, or areas of deficient concentrating ability were identifiable in nonhuman primate excretory urograms, but the diagnosis of small cysts was not possible in smaller nonhuman primates. The presence of a caliceal collecting system in the spider monkey (Ateles sp.) suggests this animal as a potential model for the evaluation of human renal disease.     

Relationship between total transit time and faecal quality in adult dogs differing in body size

Fed the same diet, large and giant-breed dogs have higher faecal moisture and increased frequency of soft stools than small ones. This could be the result of physiological differences, such as a different gastrointestinal transit time. In this study, we have correlated mean total transit time (MTT) with body size and faecal consistency in dogs varying in body size. Fifty dogs from 13 different breeds were used, from a Dachshund to a Great Dane. The MTT was determined using coloured plastic beads [Cummings and Wiggins, Gut, Vol. 17 (1976), p. 219], and faecal consistency was scored daily during the study. We confirmed the strong correlation between height at the shoulder (body size) and faecal score (r = 0.76; p < 0.0001). The MTT increased with body size, from 22 h for a Miniature Poodle to 59 h for a Giant Schnauzer. We found significant positive correlations (p < 0.0001) between MTT and body size as well as faecal scores (r = 0.71 and 0.70 respectively). In the present study, we observed an effect of body size on MTT. In our 50 healthy dogs a longer MTT was related to a poorer faecal quality. Previous studies reported no relationship between body size and the upper gastrointestinal transit time in healthy dogs. So, we hypothesized that body size would mainly affect colonic transit time and that a longer colonic residence time would be related to a poorer faecal quality by promoting fermentation activity.     

Les vitamines du groupe B dans les aliments végétaux au Sud Vietnam

Résumé Les recherches sur la teneur en vitamines du groupe B (thiamine, riboflavine, nicotinamide) faites sur 135 végétaux alimentaires du Sud Vietnam, montrent que les plantes riches en thiamine sont rares; ce sont surtout les graines et quelques rares feuilles(Sesbania grandiflora Pers. 0,365 mg% de partie comestible) et fruit (pulpe deTamarindus indica L. 0,374 mg %:Durio Zibethinus Murr. 0,300 mg %).Les bonnes sources de riboflavine sont aussi rares mais ce sont essentiellement les légumes à feuilles vertes dont spécialementIpomoea aquatica Forsk. 0,500 mg % et un champignonVolvaria esculenta Massee 0,350 mg %.Enfin la nicotinamide se trouve en quantité importante dans les plantes alimentaires étudiées et sa répartition est assez variée. Les teneurs les plus élevées se trouvent dans les graines comme:Arachis hypogea L. 12 mg %,Citrullus vulgaris Schrad. 8,450 mg %Sesamum indicum L. 5,970 mg %.

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Summary The vitamins of the B group (thiamine, riboflavine, nicotin amide) were analysed in 135 edible plants from South-Vietnam. The plants rich in thiamine are rare; some seeds, rarer leaves(Sesbania grandiflora Pers. 0.365 mg % of the edible part) and fruits (pulp ofTamarus Indica L. 0.374 mg %,Durio Zibethinus Murr 0.3 mg %) were found.The rich sources of riboflavine are equally rare; into the group belong especially green leaves, in particularIpomoea aquatica Forsk, 0.5 mg % and a fungus,Volvaria esculenta Massee 0.35 mg %.Nicotinamide is found in greater quantities in the analysed plants, the allotment being rather great. The greatest amounts have been found in the seeds:Arachis hypogea L. 12 mg %,Citrullus vulgaris Schrad 8.45 mg %Sesamum Indicum L. 5.97 mg %.

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Zusammenfassung Die Vitamine der B-Gruppe (Thiamin, Riboflavin, Nicotinsäureamid) wurden in 135 essbaren Pflanzen aus Süd-Vietnam bestimmt. Thiamin-reiche Pflanzen sind selten: manche Samen, seltener manche Blätter,(Sesbania grandiflora Pers. 0,365 mg % des essbaren Teiles) und Früchte (Fleisch vonTamarindus indica L. 0,374 mg %,Durio Zibethinus Murr 0,3 mg %).Gute Quellen an Riboflavin sind ebenso selten. Solche sind besonders grüne Blätter, namentlich vonIpomoea aquatica Forsk, 0,5 mg %, und ein Pilz,Volvaria esculenta Massee 0,35 mg %.Nicotinsäureamid findet sich in wechselnden Mengen in den untersuchten Pflanzen. Die höchsten Gehalte sind in Samen zu finden:Arachis hypogea L. 12 mg %,Citrullus vulgaris Schrad. 8,45 mg %,Sesamum indicum L. 5,97 mg %.

     

Azotemia and mortality among Babesia microti-like infected dogs

Babesia microti-like piroplasms are a recently recognized cause of illness in dogs in northwest Spain. Our objective was to describe the clinical characteristics and investigate the risk factors for azotemia and death among 58 B microti-like infected dogs. Twenty-one of the 58 (36%) dogs were azotemic at the time that the infection was diagnosed. The case fatality rate during the following week was 22%. Dogs with azotemia at the time of diagnosis were 10 times (95% CI, 3.26-28.8) more likely to die during the following week. Azotemia was the main cause of death for B microti-like infected dogs (attributable fraction = 90%). Severe anemia was present in 45 of the 58 (78%) dogs. Azotemic dogs also presented with hyperphosphatemia, hypoalbuminemia, hypercholesterolemia, proteinuria, and high urine protein: creatinine ratios, suggesting a glomerular component to the disease. Age was the only factor significantly associated with the risk of azotemia (P = .042): on average, a 4-year age increase doubled the risk of an infected dog being azotemic. The only factor significantly associated with mortality was azotemia (P = .001). We concluded that B microti-like infection is associated with a high risk of azotemia and mortality.     

Feeding of Spinach or Sweet-potato Leaves and Growth Performance of Growing Pigs Kept on Smallholder Farms in Central Vietnam

We investigated whether the addition of either spinach or sweet-potato leaves to the diet of growing pigs, kept in small holdings in Central Vietnam, would improve growth performance. A control diet was formulated and mixed with each of the vegetables to a final concentration of 15% of the total dietary dry matter. The diets were fed to the pigs from 70 to 100 days of age on six different smallholder farms in Central Vietnam. There were three animals per treatment group per farm and biopsies of adipose tissue were analysed for their contents of alpha-linolenic, eicosapentaenoic and docosahexaenoic acids. The diets without and with vegetables contained on average 0.14 and 0.32 g alpha-linolenic acid per MJ metabolizable energy. The relative percentage of alpha-linolenic acid in adipose tissue was raised by the intake of the vegetables. Eicosapentaenoic acid was not detectable in adipose tissue and the level of docosahexaenoic acid was unchanged. There was a significant stimulatory impact of the intake of either spinach or sweet-potato leaves on growth performance of the growing pigs. It is suggested that an adipose tissue content of alpha-linolenic acid less than 1% of total fatty acids does not allow maximum growth in growing pigs.