Influence of weed management measures on glyphosate resistance and endophyte infection in naturalized Italian ryegrass (Lolium multiflorum)

Recently, glyphosate‐resistant Italian ryegrass (Lolium multiflorum Lam.) was found on rice paddy levees in a western region of Shizuoka Prefecture, Japan. Naturalized populations of Italian ryegrass are frequently infected with fungal Epichloë endophytes. Endophytes often confer benefits to their host grasses. This study investigated the influence of five weed management treatments on glyphosate resistance and endophyte infection in Italian ryegrass that was growing on paddy levees where glyphosate‐resistant individuals were dominant. The weed management treatments were: (i) mowing once before the grass flowered; (ii) mowing once during flowering; (iii) mowing twice during flowering; (iv) glyphosate application before flowering; and (v) no treatment. The seeds were collected from the treatment plots in 2013 and 2014. The seeds were examined for endophyte infection and the seedlings that had been grown from the seeds were tested for the frequency of glyphosate resistance. The seedlings that had been derived from the glyphosate treatment showed higher frequencies of glyphosate resistance than those seedlings that had been derived from all the other treatments. Endophytes were found in all populations of the seeds from the paddy levees, with higher infection rates in the seeds that had been derived from the glyphosate treatment and the twice‐mowed treatment. There was a significant relationship between the endophyte infection frequency in the seeds and glyphosate resistance in the seedlings that had been grown from the same populations. The results indicate that where glyphosate herbicides are frequently used, selection for glyphosate‐resistant Italian ryegrass occurs, and along with this, the frequency of endophyte infection also increases.     

Effect of feeding sweet‐potato condensed distillers solubles on intake and urinary excretion of minerals in Japanese Black steers

Four Japanese Black steers (16 months of age) were assigned to a 4 × 4 Latin square design to investigate the effect of graded levels of sweet‐potato condensed distillers solubles (SCDS) in their diets on intake and urinary excretion of minerals. The four diets consisted of 0%, 10%, 20% and 30% (dry matter (DM) basis) SCDS, with SCDS replacing commercial concentrate (CC). Intake of K, Cl, S, P and Mg increased linearly with increasing SCDS content. Urinary pH increased linearly with increasing dietary SCDS content. SCDS feeding increased urinary K concentrations (linear and quadratic effects). Urinary concentrations of Cl increased linearly with increasing SCDS content. In contrast, urinary concentrations of Mg decreased with increasing SCDS content. Feeding of SCDS did not apparently affect urinary NH₃,P, Na or Ca concentrations. These results suggest that high SCDS feeding is not a risk for crystallization of minerals leading to the formation of magnesium‐phosphate type calculi: although SCDS contains large amounts of P and Mg, high SCDS feeding decreased the Mg concentration and did not affect the P concentration in urine. Additionally, high SCDS feeding had no apparent effects on plasma concentrations of Na, K, Cl, Ca or inorganic P.     

Effect of long‐distance transportation on serum metabolic profiles of steer calves

Long‐distance transportation is sometimes inevitable in the beef industry because of the geographic separation of major breeding and fattening areas. Long‐distance transportation negatively impacts production and health of cattle, which may, at least partly, result from the disturbance of metabolism during and after transportation. However, alteration of metabolism remains elusive in transported cattle. We investigated the effects of transportation on the metabolomic profiles of Holstein steer calves. Non‐targeted analysis of serum concentrations of low molecular weight metabolites was performed by gas chromatography mass spectrometry. Transportation affected 38 metabolites in the serum. A pathway analysis suggested that 26, 10, and 10 pathways were affected immediately after transportation, and 3 and 7 days after transportation, respectively. Some pathways were disturbed only immediately after transportation, likely because of feed and water withdrawal during transit. Nicotinate and nicotinamide metabolism, and citric acid cycle were affected for 3 days after transportation, whereas propionate metabolism, phenylalanine and tyrosine metabolism were affected throughout the experiment. Four pathways were not affected immediately after transportation, but were altered thereafter. These results suggested that many metabolic pathways had marked perturbations during transportation. Metabolites such as citric acid, propionate, tyrosine and niacin can be candidate supplements for mitigating transportation‐induced adverse effects.     

Irrelevance between the induction of anti-Campylobacter humoral response by a bacterin and the lack of protection against homologous challenge in Japanese Jidori chickens

On-farm vaccination of chickens against Campylobacter jejuni is considered a potentially effective countermeasure to decrease campylobacteriosis via consumption of contaminated chicken meat, but is not yet available. In this study, 2 groups of Jidori chicks were immunized subcutaneously with a formalin-killed C. jejuni with 2 different adjuvants. Other chicks served as the unvaccinated control group. Both vaccines induced high levels of anti-Campylobacter IgG but did not decrease bacterial excretion in cecal droppings and bacterial load in the liver and spleen after oral challenge with 10(5) CFU of the homologous strain. Further study is needed to address the observed irrelevance and to develop a novel effective vaccine against C. jejuni.     

Effect of inter-annual climate variability on evapotranspiration and canopy CO2 exchange of a tropical rainforest in Peninsular Malaysia

We investigated inter-annual variation of canopy CO₂ exchange (NEE) and evapotranspiration during a 7-year period over a lowland Dipterocarp forest in Pasoh, Peninsular Malaysia, using the eddy covariance method. Annual rainfall fluctuated between 1,451 and 2,235?mm during this period. Annual evapotranspiration estimated by energy budget correction and gap filling using the relationship between latent heat and available energy was 1,287?±?52?mm. Despite inter-annual variation in rainfall, annual evapotranspiration was stable, except for a slight decrease in the driest year (2009). Evapotranspiration was roughly related to the amount of available energy, but was regulated by stomatal closure to prevent excessive water loss at high vapour pressure deficit. Even during dry periods, no significant decrease in evapotranspiration occurred, as water was supplied from soil layers deeper than 0.5?m. Ecosystem respiration (RE) increased with soil water content. Daytime NEE was also stable during the 7?years, despite climate variability. Afternoon inhibition of canopy photosynthesis was seen every month. Daytime NEE did not become more negative with increasing solar radiation, or with increasing soil water content. During dry periods, gross primary production (GPP) and thus canopy gross photosynthesis decreased slightly, coupled with decreased daytime RE. In this forest, variability in rainfall pattern resulted in seasonal and inter-annual variability in micrometeorology; evapotranspiration, photosynthesis, and RE responded to these changes, and compensated for each other and/or other components of micrometeorology, resulting in rather stable annual evapotranspiration and NEE, even during a very dry year associated with an El Nino Southern Oscillation (ENSO) event.     

Epizootic canine distemper virus infection among wild mammals

In the spring of 2007, seven raccoon dogs and a weasel were captured near the city of Tanabe in Wakayama prefecture, Japan. The causative agent of the animals' death 1-2 days after capture was identified as canine distemper virus (CDV) by virus isolation, immunostaining with an anti-CDV polyclonal antibody, and a commercially available CDV antigen-detection kit. Sequence analysis of hemagglutinin genes indicated the isolated viruses belong to genotype Asia-1 and possess the substitution from tyrosine (Y) to histidine (H) at position 549 that is associated with the spread of CDV to non-canine hosts. A serosurvey for CDV was then conducted among wild animals in the region. The animals assayed consisted of 104 raccoons, 41 wild boars, 19 raccoon dogs, five Sika deer, two badgers, one weasel, one marten, one Siberian weasel and one fox. Virus-neutralization (VN) tests showed that, except for fox and weasel, all of the species assayed had VN antibodies to CDV. Interestingly, 11 of the 41 wild boars (27%) and two of the five Sika deer assayed possessed VN antibodies to CDV. These findings indicate that CDV infection was widespread among wild mammals during this epizootic.     

Efficacy of soluble recombinant FliC protein from Salmonella enterica serovar enteritidis as a potential vaccine candidate against homologous challenge in chickens

FliC, the flagellin antigen of Salmonella Enteritidis, was tested as a vaccine candidate for protective effect against a homologous challenge in chickens. After immunization with recombinant FliC (rFliC) or administration of phosphate-buffered saline (PBS) at 56 days old, the chickens were challenged with 10(9) colony-forming units of Salmonella Enteritidis at 76 days old. The vaccinated birds showed significantly decreased bacterial counts in the liver and cecal contents compared to those administered PBS at 7 days postchallenge, but the protection was partial. The replication experiment also showed a similar result. In both experiments, vaccination induced an increased level of serum anti-rFliC IgG, which was also reactive to the native flagella. The intestinal IgA level was slightly higher in the vaccinated birds than in the control. However, neither the proliferative response nor interferon-gamma secretion of splenic cells upon stimulation with rFliC was induced. Therefore, the effect of rFliC as a vaccine is limited, and further improvement is needed.     

Surgical correction of subvalvular aortic stenosis using cardiopulmonary bypass in a dog

A three-month-old male Golden Retriever had symptoms including exercise intolerance, dyspnea and syncope and was diagnosed with subvalvular aortic stenosis. Cardiac catheterization revealed a left ventricular-aortic systolic pressure gradient of 90 mm Hg. Surgical correction of the condition was achieved using cardiopulmonary bypass. The subvalvular fibrous lesion was resected through an aortotomy. The stenosis was dilated from 8.5 mm to 12.0 mm in diameter. Postoperatively the dog was asymptomatic. Seven months after surgery, the pressure gradient decreased to 44 mm Hg. However, after another three months, the dog died suddenly without any premonitory signs. Postmortem examination revealed that pathologic changes caused by increased left ventricular pressure overload were not severe.     

The reference values of the T-wave of the patellar tendon reflex in normal dogs

The T-wave of the patellar tendon reflex (PTR) was recorded in 24 neurologically normal dogs. The surface electromyogram (EMG) was recorded as the T-wave from the vastus lateralis muscle (VL) in response to percussion of the patellar tendon. The distance of the reflex arc (DRA) was measured along the straight line between the spinous process of L5 and the greater trochanter (GT), and between GT and the patellar ligament (PL). There was a significant correlation (P<0.001) of the latency with the DRA on each side, but no difference in the slopes of the relationships between right and left VL was shown. The regression line between the DRA and the latency of all data was Y = 0.0216X + 1.693, where Y = latency in ms, X = DRA in mm. The mixed sensory-motor conduction velocity was estimated as 84.6 +/- 5.5 m/s. In contrast, there was no significant correlation between the DRA and the amplitude of the T-waves. The mean (mean-CV) and standard deviation (SD-CV) of all CV (coefficient of variation) in each dog were 9.14 +/- 3.65% in latency and 3.54 +/- 1.14% in amplitude, indicating that the use of a simple hand-held reflex hammer is sufficient to record the reproducible T-wave of the PTR even in unanesthetized dogs. This method was applied to a case with minimal paraparesis, and the latency of the T-wave of the PTR in the right hind limb with slight proprioceptive deficit was outside of the upper limit of the 95% confidence interval between latency and the DRA. In conclusion, this method may be used in neurological diagnosis to quantify more precisely the PTR in dogs.