全文获取类型
收费全文 | 258篇 |
免费 | 6篇 |
专业分类
林业 | 5篇 |
农学 | 21篇 |
基础科学 | 1篇 |
28篇 | |
综合类 | 15篇 |
农作物 | 5篇 |
水产渔业 | 18篇 |
畜牧兽医 | 142篇 |
园艺 | 4篇 |
植物保护 | 25篇 |
出版年
2022年 | 6篇 |
2021年 | 9篇 |
2020年 | 3篇 |
2019年 | 1篇 |
2018年 | 4篇 |
2017年 | 8篇 |
2016年 | 6篇 |
2015年 | 3篇 |
2014年 | 11篇 |
2013年 | 16篇 |
2012年 | 14篇 |
2011年 | 16篇 |
2010年 | 5篇 |
2009年 | 7篇 |
2008年 | 16篇 |
2007年 | 21篇 |
2006年 | 14篇 |
2005年 | 13篇 |
2004年 | 14篇 |
2003年 | 15篇 |
2002年 | 9篇 |
2001年 | 3篇 |
2000年 | 7篇 |
1999年 | 11篇 |
1998年 | 2篇 |
1997年 | 4篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1984年 | 3篇 |
1982年 | 2篇 |
1981年 | 5篇 |
1980年 | 1篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1974年 | 1篇 |
1973年 | 2篇 |
排序方式: 共有264条查询结果,搜索用时 15 毫秒
101.
Noriyuki Miyake Hirofumi Nagai Shinro Kato Masashi Matsusaki Shiro Fukuta Reiko Takahashi Ryoji Suzuki Yasushi Ishiguro 《Journal of General Plant Pathology》2017,83(1):1-6
Two detection methods combining loop-mediated isothermal amplification (LAMP) and a bait trap were developed to detect Pythium helicoides in greenhouses containing roses, miniature roses, and poinsettias in hydroponic culture systems. In “Bait-LAMP”, a crude extract derived from perilla seeds as the bait was used in the LAMP reaction, whereas in the “Bait culture-LAMP”, a crude extract of mycelia grown out from perilla seeds onto Pythium-selective medium served as the bait. The two methods are simple and rapid for practical monitoring of P. helicoides in hydroponic culture systems. 相似文献
102.
Sachiko Ikeda Tamotsu Hoshino Naoyuki Matsumoto Norio Kondo 《Journal of General Plant Pathology》2017,83(1):46-50
Typhula winter rot on overwintering carrots caused by Typhula variabilis is a newly confirmed disease, and no practical control measure is yet available. To develop a control method, here we researched the infection period of T. variabilis and the time that winter rot appeared on carrots. Using spore traps, we found that basidiospore rain occurred from September to November before snowfall in Memuro, Hokkaido. In addition, carrot leaves collected in autumn had already been infected by T. variabilis. These epidemiological investigations revealed that the pathogen releases basidiospores to infect carrot leaves before snow cover, resulting in root decay under snow. An effective control method was then developed to avoid direct contact of basidiospores of T. variabilis with plant tops by covering the plants with soil in autumn. Thus, the percentage of rotted roots was reduced to about half. 相似文献
103.
Genetic characteristics of four wild (Mie, Fukui, Shimane and Nagasaki) and five cultured populations (selectively bred for 12 years with an origin of Ehime population) of the Japanese pearl oyster, Pinctada fucata martensii, were evaluated by using AFLP markers. Six primer pairs generated 1019 loci in total, among which 45.2–55.1 % was polymorphic among populations. Although there was no significant difference in gene diversity between wild (0.170–0.174) and cultured (0.158–0.173) populations, genetic relatedness in cultured populations (0.316–0.450) was about three times higher than that in wild populations (0.110–0.165). In addition, genetic differentiation was about twenty times larger in cultured populations (Nei’s distance: 0.0111) than in wild populations (Nei’s distance: 0.0005). These results mean that selective breeding can cause marked inbreeding as well as large genetic differentiation among cultured populations in a short period. On the other hand, it was suggested that genetic homogenization in the wild, probably due to a large-scale transport of cultured oysters, had progressed in the sea around Japan. In conclusion, it is necessary to prevent inbreeding by the reconsideration of the style of selective breeding in cultured populations, while the escape of gametes or spats of cultured strains in the wild should be avoided for the preservation of genetic characteristics in native populations of P. f. martensii. 相似文献
104.
Shohei Matsuura Yosuke Matsushita Reiko Kozuka Sachiko Shimizu Shinya Tsuda 《European journal of plant pathology / European Foundation for Plant Pathology》2010,126(1):111-115
Quantitative PCR revealed that Tomato chlorotic dwarf viroid (TCDVd) was present in substantial amounts in viroid-infected tomato flowers. Healthy tomato plants were arranged in two
different glasshouses, and plants were mechanically inoculated with TCDVd. Bumblebees (Bombus ignitus) were then introduced into the glasshouses to reveal whether the viroid was transmitted from infected source plants to neighbouring
healthy plants. TCDVd infection was found in neighbouring tomato plants more than 1 month after the introduction of the bees,
some of which expressed symptoms, in both glasshouses. Thus, bumblebees transmitted TCDVd from tomato to tomato by pollination
activities. 相似文献
105.
The identification of homogeneous management zones (MZs) within a field is a basis for site-specific management (SSM). We
assessed the method of defining MZs based on the spatio-temporal homogeneity of six soil properties and above-ground biomass
data from paddy rice, winter wheat and soybean over 3 years on a farm with 124 contiguous small paddy fields. The soil data
were recorded at 372 soil sampling sites on a rectangular grid over the farm. A non-hierarchical cluster analysis was applied
to the soil data and the algorithm grouped the sites into three clusters with similar soil properties. These clusters represent
soil fertility and soil drainage. The three clusters were not randomly distributed across the fields, but formed contiguous
areas associated with landscape position. This was due to the spatial variation of the soil in the study area. We delineated
five MZs based on the spatial structure of the soil heterogeneity of the study area. The validity of the MZs was evaluated
using the biomass data from paddy rice, winter wheat and soybean in each MZ; this depended mainly on soil fertility when conditions
were dry. When the growing season precipitation was greater than the 10-year average, the biomass of winter wheat and soybean
depended on soil drainage. This suggested that the delineation of MZs for site-specific management in fields under a paddy-upland
crop rotation system should be based on several soil properties. The biomass data from the three crops over 3 years was not
effective for delimiting MZs. 相似文献
106.
Y Miyamoto R Sakumoto Y Sakabe M Miyake A Okano & K Okuda 《Reproduction in domestic animals》2002,37(2):105-110
To determine the physiological significance of tumour necrosis factor‐α (TNFα) in the regulation of luteal functions in pig, this study was conducted to identify the presence of functional TNFα receptors in porcine corpora lutea (CL) throughout the oestrous cycle and the early gestation. The CL were isolated from pigs on days 4, 6, 8, 12 or 15 of the oestrous cycle (n=3; day 0 = oestrus) and days 15, 20 or 25 of gestation (n=3; day 0 = mating). A Scatchard analysis revealed the presence of a high‐affinity binding site for TNFα in all samples (dissociation constant; 2.7 ± 0.51 to 5.8 ± 0.50 nM ). The concentration of TNFα receptors was higher on day 15 of the oestrous cycle than on days 4 and 8 of the oestrous cycle (p < 0.05). Furthermore, TNFα receptor concentrations in the CL on days 15, 20 and 25 of gestation were significantly lower than on day 15 of the oestrous cycle (p < 0.05). On day 9 of the oestrous cycle, exposure of cultured luteal cells to 0.06–60 nM TNFα stimulated prostaglandin (PG) F2α and PGE2 secretion in a dose‐dependent manner (p < 0.05). These results indicate that functional TNFα receptors are present in the porcine CL throughout the oestrous cycle and early gestation, and suggest that TNFα plays one or more physiological roles in regulating CL function throughout the oestrous cycle and the early gestation period. In addition, TNFα receptor concentration in the CL of the late luteal stage (day 15) of the oestrous cycle was higher than on the respective day in the early pregnant pig, suggesting that TNFα plays a role in accomplishing luteolysis in the porcine CL. 相似文献
107.
108.
Derar RI Maeda Y Hoque SM Osawa T Watanabe G Taya K Miyake Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(1):31-35
Two pony mares were immunized against recombinant porcine inhibin alpha subunit three times with 39 day intervals. Clinical findings and endocrinological changes before immunization were taken as the control. The first significant rise in the anti-inhibin titre (P<0.05) in the circulation was found 27 days after the first injection. Maximum binding activity was reached by the 12th day after the second booster dose. The number of small, medium and large sized follicles had increased significantly compared to before immunization (11.75 +/- 4.30, 2.75 +/- 0.69 and 2.51 +/- 0.63 vs 6.50 +/- 1.43, 1.83 +/- 0.44 and 1.33 +/- 0.38, respectively), but the ovulation rate remained unchanged after immunization. The average plasma concentration of FSH and estradiol-17beta during the estrous cycle increased significantly (P<0.05) after immunization. These results suggest that immunization against inhibin is a useful tool to increase the number of ovarian follicles during the estrous cycle of pony mares. Moreover, the present study supported the concept that inhibin plays a major role in the control of follicular growth through its inhibitory effect on FSH secretion synergistically with steroid hormones. 相似文献
109.
Miyake M Yaguchi T Saze K Wang J Ogawa T Endo Y Suzuta Y Okazaki M Haga Y Waki T Takahashi SY Yamamoto Y Iwabuchi S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(1):9-14
Hepatocyte growth factor (HGF) is a pleiotropic cytokine that stimulates a wide array of cellular targets, including hepatocytes and other epithelial cells, melanocytes, endothelial and hematopoietic cells. We have cloned a different form of cDNA, with a deletion of 15 base pairs predicted to result in the loss of 5 amino acids from the first kringle domain. To investigate the biological activity, original and deleted variant of feline HGF cDNAs were transiently expressed in COS-7 cells. Both recombinant feline HGFs showed almost the same dose-response curves in the stimulation of the growth of BNL CL.2 cells (a mouse hepatocyte cell line) and scatter activity of Madin-Darby canine kidney (MDCK) cells. The findings reported here suggest that the deleted variant of feline HGF has almost the same biological activity as the original in terms of the proliferation and scatter activity. 相似文献
110.
Kaneko K Aoki H Furuichi T Hatori S Tanimoto H Kawakami S 《The Journal of reproduction and development》2004,50(3):361-367
To investigate how uterine inflammation affects ovarian activity in rats, endometritis was induced and changes in the length of estrous cycle and serum concentrations of estradiol-17beta (E(2)) and progesterone (P(4)) were examined. A suspension of Staphylococcus aureus (bacterial solution) or iodine solution was infused into the uterine lumen at various estrous phases. When the bacterial solution was infused at estrus, metestrus, or the first day of diestrus, the following diestrus continued for 5 to 12 days. In the case of the iodine solution, regardless of the estrous phase of the infusion, the following diestrus continued for approximately 6 days. E(2) concentration after infusion of each solution did not fluctuate largely and remained at a low concentration (around 5 pg/ml). P(4) concentration was high (35-45 ng/ml) on the day following infusion, but decreased rapidly to base line values within a few days and remained thereafter at a low level (around 5 ng/ml). It is assumed that the endometritis caused by biological or chemical stimulation raises the concentration of P(4) to depress gonadotrophic hormone secretion, and hence this high P(4) concentration might inhibit the growth of ovarian follicles. 相似文献