Nitrogen and Its Effect on Growth, Nutrient Status and Parasite Attacks in Beech and Norway Spruce

In permanent observation plots across Switzerland, nitrogen (N) concentration in the foliage of mature beech has increased by 15% and phosphorus (P) concentrations in beech and mature Norway spruce decreased by 12 and 13% respectively between 1984 and 1995, leading to increased N:P ratios. Modelled N deposition was correlated with stem increment in both beech and spruce, with indications of P limitation in some beech plots. Experimental application of 0-160 kg N ha^-1> yr^-1> over four to five years caused nutrient imbalances in various afforestation plots comparable to those observed in the permanent observation plots. The changes in the trees caused by N treatment led to increased attacks by parasites such as Apiognomonia errabunda, Phomopsis sp., Phyllaphis fagi in beech and Botrytis cinerea, Sacchiphantes abietis and Cinara pilicornis in Norway spruce. The results suggest current N deposition in Switzerland induces significant changes in the forest ecosystem.     

Ontology-based simulation in agricultural systems modeling

A methodology and applications of ontology-based simulation are presented. An environment for building simulations based on the Lyra ontology management system is described which includes web-based visual design tools for constructing models and automatically generating simulation code. The ontology is used for representing all equations and all symbols appearing in these equations that are needed to describe a model. The example applications presented are models of soil, water, and nutrient management in citrus and sugarcane. Results thus far show that the ontology-based approach has advantages for representing the model structure, equations, and symbols, that complex models can be described in this format, and that efficient simulation code can be generated automatically from the ontology definition of the model. Potential applications, not yet fully explored, include ability to automatically connect models and data sources, using the ontology to organize model bases containing many models and model components, and using ontology reasoners to search for models, automatically discover model similarities and differences, and generate model instances from general principles.     

Influence of Compost Addition to Soil on the Behaviour of Herbicides

The transformations of eight herbicides (atrazine, simazine, terbutryn, pendimethalin, carbetamide, 2,4-D, metsulfuron-methyl and dimefuron) in soil after compost addition were monitored during long-term laboratory incubations. The herbicides were applied to soil, compost and soil-compost mixtures. Herbicide sorption, their kinetics of mineralisation and the extractability of residues were compared in the different treatments. Compost addition to soil generally decreased herbicide mineralisation and favoured the stabilisation of herbicide residues. A fraction of the stabilised residues remained extractable and potentially available. However, most of them were unextractable and formed bound residues. Sorption could be at the origin of a kinetically limited biodegradation, mainly for the most highly-sorbed herbicides (atrazine, simazine, terbutryn, pendimethalin and dimefuron). Compost addition had little effects on the less sorbed herbicides (carbetamide, 2,4-D and metsulfuron- methyl). © 1997 SCI.     

Exposure assessment of professional pesticide users during treatment of potato fields

In this study four different mixing/loading and application practices in potato fields were monitored for exposure of operators to pesticides. Each operation – mixing, loading, and application – was measured individually in order to assess its relative contribution to the total exposure value. Inhalation exposure was measured by trapping the pesticides with a sorbent tube while sampling the air around the operator's face. Dermal deposition, which was measured by means of cotton gloves on the hands and by attaching patches to the operator's clothing, was the main contributor to the total exposure. Dermal deposition on the hands during mixing and loading exceeded all other dermal values. The experimental results are compared with the results obtained by the exposure assessment model PHED V1.1. This model gives an underestimation of the levels of operator exposure during mixing, loading and application. © 1999 Society of Chemical Industry     

Growth,survival rate and fatty acid composition of sterlet (Acipenser ruthenus) larvae fed fatty acid‐enriched Artemia nauplii

We evaluated the growth and survival rate of sterlet (Acipenser ruthenus) larvae fed Artemia nauplii enriched with Olioω3 or Red Pepper commercial emulsions (BernAqua NV, Belgium). Sterlet larvae, 0.022 ± 0.002 g body weight, were randomly assigned to one of three feeding regimes with two different feeding durations. After administering live feed for 7 or 14 days, larvae were weaned onto commercial food and reared to 36 days posthatching (28 days of feeding). There were no significant differences in body weight among groups at the end of the trial. A significantly higher survival rate (p < 0.05) was observed in larvae fed Artemia enriched with Red Pepper for 14 days compared to other feeding regimes. Based on the analysis of growth parameters, we can conclude that 7 days of live feeding to be sufficient for efficient rearing of sterlet larvae. And longer duration of live feeding with use of special enrichment can be recommended for a higher survival rate.     

Rapid measurements of fat content in live and slaughtered common carp (<Emphasis Type="Italic">Cyprinus carpio</Emphasis> L.)

The objective of this study on common carp (Cyprinus carpio) was to obtain and predict the first cross-validated data of the fat content on market size carps using a non-invasive or non-destructive method in situ. The carps (1680 ± 388 g; n = 136) used were from a semi-intensive system and were on a different diet (cereal, pelleted and extruded diet). For the evaluation of the fat content, a Fish Fatmeter FM 692 from Distell.com. (FFM) and a manual measurement of back fat height using a digital calliper were used. For the prediction model, the following basic body measurements (variables) were used: total body length, body length, body height, the width of the body, and the circuit of the body. The body weight, weight of intestines, weight of gonads, weight of hepatopancreas, and fillet yield (%) were measured, and the Fulton coefficient was calculated. The study was focussed on evaluating the applicability of these methods and the accuracy of the obtained result, respectively. Results showed that all the rapid methods had a strong correlation. Multiple regression models with forward selection of variables were used throughout. The final prediction model between predicted and observed values for the fat content for FFM and calliper being adjusted index of determination is shown here (R _adj ²  = 0.88; 5 variables and R _adj ²  = 0.91; 7 variables), respectively.     

Chlorogenic acid,a metabolite identified by untargeted metabolome analysis in resistant tomatoes,inhibits the colonization by Alternaria alternata by inhibiting alternariol biosynthesis

Tomato fruits can be contaminated by saprophytic strains of Alternaria alternata which is the reason for the frequent occurrence of Alternaria toxins like alternariol, alternariol monomethylether or tenuazonic acid in these types of products. It was shown earlier that alternariol is a colonization factor for tomatoes. In the current analysis two different tomato genotypes were analysed by untargeted comprehensive two-dimensional gas chromatography mass spectrometry (GC×GC-MS). This analysis revealed clear differences in the metabolic profiles which were paralleled by differences in resistance towards Alternaria colonization. One of the genotypes was more resistant against A. alternata infection and contained high amounts of chlorogenic acid in contrast to the other genotype which was sensitive against infection. In in vitro analysis, chlorogenic acid reduced alternariol biosynthesis during the first days of growth of A. alternata. Expression analysis of the alternariol polyketide synthase gene, a key gene in the biosynthesis of alternariol, also revealed a temporal reduction in its expression in the first phases of growth. However by chromatographic analysis it could be demonstrated that chlorogenic acid was degraded over time. This degradation leads to a relief of inhibition resulting in an only temporal inhibition of alternariol biosynthesis. In vivo colonization experiments revealed that chlorogenic acid reduces colonization of tomatoes by A. alternata in a concentration dependent manner, which however is partly counteracted by the addition of alterariol.     

Effect of tillage intensity on weed infestation in organic farming

Conservation tillage is not yet widely accepted by organic farmers because inversion tillage is considered to be necessary for weed control. Three long-term experiments were established with combinations of reduced and conventional plough tillage and stubble tillage to determine weed infestation levels in organic farming, i.e. herbicide application being excluded. Experiment 1 (with very low stocking density of perennial weeds) showed that in presence of primary tillage by mouldboard ploughing the number of annual weeds was nearly unaffected by the mode of stubble tillage. In experiment 2, however, with Canada thistle (Cirsium arvense) being artificially established, thistle density was significantly affected by stubble tillage and by a perennial grass–clover forage crop. Experiment 3 combined two levels of stubble tillage (skimmer plough, no stubble tillage = control) with four implements of primary tillage in the order of decreasing operation depth (deep mouldboard plough, double-layer plough, shallow mouldboard plough or chisel plough). Primary tillage by chisel plough resulted in significantly highest annual weed density compared to all other treatments. The natural C. arvense infestation in experiment 3 showed highest shoot density in the “skimmer plough/chisel plough” treatment compared to the lowest infestation in the “skimmer plough/double-layer plough” treatment. The poor capacity of the chisel plough for weed control was also reflected by the soil seed bank (5500 m⁻² C. arvense seeds for chisel plough, <300 seeds for all other primary tillage). A reduced operation depth of the mouldboard plough (“shallow mouldboard plough”) seemed to have an insufficient effect in controlling C. arvense infestation as well. Stubble tillage by the skimmer plough in addition to nearly any primary tillage operation largely reduced both annual weeds and thistle shoots. Most effective in controlling C. arvense was also a biennial grass–clover mixture as part of the crop rotation.Double-layer ploughing is a compromise between soil inversion and soil loosening/cutting and can be regarded as a step towards conservation tillage. In terms of controlling annual weeds and C. arvense, the double-layer plough was not inferior to a deep mouldboard plough and seems to be suitable for weed control in organic farming. Tilling the stubble shallowly after harvest can support weed control in organic farming remarkably, particularly in reducing C. arvense. If no noxious, perennial weeds occur and primary tillage is done by soil inversion, an omission of stubble tillage can be taken into consideration.