全文获取类型
收费全文 | 880篇 |
免费 | 41篇 |
国内免费 | 96篇 |
专业分类
林业 | 100篇 |
农学 | 145篇 |
基础科学 | 64篇 |
152篇 | |
综合类 | 230篇 |
农作物 | 31篇 |
水产渔业 | 35篇 |
畜牧兽医 | 160篇 |
园艺 | 35篇 |
植物保护 | 65篇 |
出版年
2024年 | 3篇 |
2023年 | 10篇 |
2022年 | 31篇 |
2021年 | 30篇 |
2020年 | 29篇 |
2019年 | 33篇 |
2018年 | 25篇 |
2017年 | 25篇 |
2016年 | 38篇 |
2015年 | 32篇 |
2014年 | 32篇 |
2013年 | 39篇 |
2012年 | 62篇 |
2011年 | 55篇 |
2010年 | 54篇 |
2009年 | 42篇 |
2008年 | 46篇 |
2007年 | 50篇 |
2006年 | 38篇 |
2005年 | 54篇 |
2004年 | 26篇 |
2003年 | 17篇 |
2002年 | 12篇 |
2001年 | 15篇 |
2000年 | 27篇 |
1999年 | 28篇 |
1998年 | 21篇 |
1997年 | 25篇 |
1996年 | 17篇 |
1995年 | 20篇 |
1994年 | 13篇 |
1993年 | 17篇 |
1992年 | 11篇 |
1991年 | 8篇 |
1990年 | 10篇 |
1989年 | 9篇 |
1988年 | 4篇 |
1987年 | 2篇 |
1985年 | 2篇 |
1978年 | 1篇 |
1975年 | 1篇 |
1965年 | 1篇 |
1958年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有1017条查询结果,搜索用时 15 毫秒
101.
Itai Barnoon Orit Chai Itai Srugo Dana Peeri Lilach Konstantin Ori Brenner Merav H. Shamir 《The Canadian veterinary journal. La revue veterinaire canadienne》2012,53(11):1191-1194
Myelo-computed tomography of a paraparetic 14-year-old dog revealed subarachnoid distension with an intradural filling defect above the T13–L1 disc space. T12–L1 hemilaminectomy followed by durotomy allowed removal of a large piece of degenerated disc material that compressed the spinal parenchyma. Full return to function was achieved 10 days post-surgery. The distension was likely secondary to the intradural herniation, and is a rare and distinct finding. 相似文献
102.
Liu J Zhou D Cheng Z Wang Z Wang L Wang S Yang D Chai T 《Research in veterinary science》2012,93(1):48-50
The recombinant ppa protein of Mycoplasma suis migrated to 21 kDa. Using this antigen, an ELISA system to detect the antibody against M. suis infection in swine was established. The rELISA demonstrated 98.5% specificities among negative samples and 96.9% sensitivity among positive samples with M. suis infection. A comparison of this ELISA system with an indirect hemagglutination assay (IHA) test using 132 swine samples revealed that the positive rate was 34.0% in ELISA and 28.0% in IHA. Compared with IHA, the present rELISA system using recombinant ppa antigen significantly improves the specificity, sensitivity, and stability for serodiagnosis of M. suis infection in swine. 相似文献
103.
104.
在研制猪细小病毒灭活疫苗时,应用了超滤浓缩技术,提高了合毒细胞培养液中的抗原含量。为了保证浓缩的流量,减少对滤膜的污染,对含毒细胞培养液作了预处理一离心及微滤,以便除去细胞培养液中的细胞碎片、蛋白质等大分子物质及固形微粒。超滤时选用50000Da滤膜,在20psig压力下,经过5倍浓缩的PPV细胞培养液,血凝滴度提高2个滴度以上。病毒含量测定表明,TCID50/0.2mL由107左右升至109以上,以其配制疫苗,能显著地提高疫苗的免疫原性。超滤技术具有易于操作、高效、分离精度高、没有二次污染等优点,可以根据需要选择不同的浓缩浓度,对保证疫苗的质量具有重要作用。 相似文献
105.
106.
通过提取动脉脉搏波特征参数,建立了间接测定大鼠动脉血压的多元回归方程。结果表明:脉搏波周期、K值、脉搏波波图面积等参数与动脉收缩压有较好的相关性,与颈动脉有创血压测定法数值比较,有较小差异(P>0. 05),表明所筛选的脉搏波特征参数能准确地反映动脉的每搏收缩压值,但与舒张压相关性较差。该方法简便、准确、稳定性好,便于长期动态观察。 相似文献
107.
CIMMYT 145份小麦种质资源的鉴定及杂交利用 总被引:3,自引:3,他引:0
为了拓宽和丰富小麦种质资源遗传多样性,提高小麦产量和品质,在大田对国外引进的145份小麦种质资源进行了农艺性状和品质性状的鉴定和评价。结果表明,这些材料基本属弱春性类型。在山西南部麦区,可推迟播期于10月下旬种植,作为育种材料利用。主要农艺性状的单株穗数、穗粒数、千粒重和产量变异系数分别为15.7%、16.03%、12.23%和23.96%,多数材料籽粒综合品质较好,尤其面筋品质好,筋力强。这些材料的农艺性状和品质性状具有丰富的遗传多样性。株高和千粒重与产量的相关系数最大,分别为0.5032和0.4178,达极显著水平,是提高产量的关键因素。综合3年试验结果,筛选出产量超过3750 kg/hm2有32份,鉴选出8个组合的相应强筋力品系和35个综合性状表现较优异品系,其突出优异性状表现植株穗大,小穗结实好,穗粒多,可作为小麦育种的重点种质材料利用。通过播期调节花期,利用CIMMYT材料合理杂交配组,已选育至F3代、BC2F1代和BC1F1代,有望育成优质大穗丰产抗旱创新材料及新品系。 相似文献
108.
109.
Wen-Ying Wang Wei-Wei Chai Chang-Yu Zhao Owen Rowland Bao-Shan Wang Xin Song Ya-Qi Liu Qing Ma Suo-Min Wang 《植物养料与土壤学杂志》2019,182(4):597-606
Zygophyllum xanthoxylum is a salt‐accumulating xerophytic species with excellent adaptability to adverse environments. Previous studies demonstrated that Z. xanthoxylum absorbs a great quantity of Na+ as an osmoregulatory substance under arid conditions. To investigate the nutritional status of Z. xanthoxylum in comparison with a typical glycophyte, Arabidopsis thaliana, seedlings were exposed to NaCl (50 mM for Z. xanthoxylum and 5 mM for A. thaliana), osmotic stress (–0.5 MPa), and osmotic stress combined with the NaCl treatment. Compared to the control, NaCl treatment or osmotic stress significantly increased Na+ concentration in leaves and roots of Z. xanthoxylum, but not of A. thaliana. Under osmotic stress, the addition of NaCl significantly increased Na+ concentration in leaves and roots of Z. xanthoxylum, resulting in improved biomass and tissue water content. However, such changes were not observed in A. thaliana. Compared to the control, K+ concentrations in leaves and roots remained unchanged in Z. xanthoxylum when exposed to osmotic stress, with or without additional 50 mM NaCl. In contrast, significant reductions in shoot K+ concentrations of A. thaliana were observed under osmotic stress alone or when combined with 5 mM NaCl. Moreover, NaCl alone or when combined with osmotic stress enhanced the accumulation of N, P, Fe, Si, Ca2+, and Mg2+ in Z. xanthoxylum, but did not cause such nutritional changes in A. thaliana. Compared to the glycophyte A. thaliana, Z. xanthoxylum could accumulate Na+ and maintain the stability of nutritional status at a relatively constant level to cope with drought stress. 相似文献
110.
Hardwoods contain a substantial amount of hemicellulose-type oligosaccharides, the chemical structures of which are typically categorized in the xylan saccharide class. The efficient and selective isolation of these hemicelluloses is regarded as one of the most critical obstacles to overcome for their eventual biomaterial and bioenergy utilization. Therefore, the objective of the current work was to perform a fundamental exploration of the function of extraction pH, temperature, and time on the final chemical properties of the extracted hemicelluloses. The extraction chemistries employed varied pH and showed that acidic conditions provided a higher extractive yield versus alkaline conditions. An alkaline environment gave higher yields than an acidic environment and also resulted in much higher lignin removal from wood, especially at high temperature. In general, control of the pH chemistry leads to a change in the carboxylic acid groups present in the extracts. When the extraction pH changed from acidic to alkaline, the total carboxylic acid group content in the hemicelluloses removed significantly increased due to more acetic acid group hydrolysis and increased lignin degradation. 相似文献