Further evidence that zinc sulphate compromises the efficacy of dipping treatments using diazinon to control sheep lice (Bovicola ovis)

Objective To compare the wettability and efficacy of diazinon dip wash made with and without the addition of zinc sulphate.
Design Field experiments using a shower and a plunge dip complemented by in-vitro wettability experiments.
Procedure A flock of infested sheep was divided into groups and treated in a shower dip with clear or cloudy dam water plus up to 1.5% zinc sulphate. Another infested line of sheep was treated using a plunge dip with nil or 1% zinc sulphate. In both experiments, wetting was assessed after dipping and louse counts were conducted for 9 months after treatment. Five in-vitro experiments compared the wettability of dip wash containing diazinon with up to 1.5% zinc sulphate added.
Results In the shower dipping experiment, live lice were found at 1 month after dipping in the cloudy water groups with 0.75%, 1.0% and 1.5% zinc sulphate and at 2 months in the 0.75% zinc sulphate group. No lice were found at subsequent inspections or at any time in the groups that were plunge dipped. Zinc sulphate decreased the amount of dip wash retained by wool staples in all in-vitro experiments (P < 0.05).
Conclusion Zinc sulphate should be considered as a risk factor that could cause failure to eradicate a lice infestation. The risk can be overcome by ensuring that all sheep are saturated at dipping and that the dip wash, and any holding tanks, are agitated throughout the dipping event.     

Expression of Genes in the Canine Pre‐implantation Uterus and Embryo: Implications for an Active Role of the Embryo Before and During Invasion

The aim of the present study was to assess genes expressed in maternal uterine tissue and pre‐implantation embryos which are presumably involved in maternal recognition and establishment of canine pregnancy. For this purpose, 10 pregnant bitches were ovariohysterectomized between days 10 and 12 after mating. Four non‐pregnant bitches served as controls. Early pregnancy was verified by flushing the uterine horns with PBS solution. The collected embryos (n = 60) were stored deep‐frozen (?80°C). Uterine tissue was excised, snaps frozen in liquid nitrogen and homogenized using TRI Reagent. All embryos from one litter were thawed together and also homogenized in TRI Reagent. RT‐PCR was performed to prove mRNA expression of progesterone receptor, key enzymes of the prostaglandin synthesis pathway, selected growth factors, cytokines, immune cell receptors, major histocompatibility complex (MHC) and matrix‐metalloproteinases (MMP). Only pregnant uteri revealed the presence of mRNA for interferon (IFN)‐γ, IL‐4 and CD‐8, which resembles the milieu in humans and other mammalians. Similarly, in day 10 embryos, mRNA for transforming growth factor‐β, insulin‐like growth factor‐1,‐2, hepatocyte growth factor, leukaemia inhibitor factor, tumour necrosis factor‐α, interleukin‐1β,‐6,‐8, cyclooxygenase‐2, CD4⁺ cells, and MMP‐2 and ‐9 were detected, but not MHC‐I or ‐II. We therefore suppose that the canine embryo, like its human counterpart, actively initiates measures to prevent attacks from the maternal immune system to prepare its own adhesion, nidation, growth and further development.     

Osseous lesions resembling vaccine-site sar comas in two cats

Extract

Lytic lesions with microscopic features similar to those of vaccine-site sarcomas were diagnosed in the distal femur of two young neutered male cats, one a 20-month-old Birman and the other an 18-month-old domestic short-hair (DSH). Radiographically, there was extensive destruction of trabecular and cortical bone in the metaphysis and/or epiphysis of the distal femur. The DSH cat also had angular deformity of the distal femur due to asymmetrical involvement of the physis. Histologically, the lesions in both cats consisted of dense, interwoven bundles of fibrous connective tissue, amongst which there were variable numbers of plump malignant mesenchymal cells, often with very large, hyperchromatic nuclei and irregularly shaped nucleoli. Several focal aggregates of small lymphocytes were present in both tumours. Although neither cat had apparently been vaccinated in the hindlimb it is possible that the tumours had been triggered by injection of some other irritant material.     

The effect of a cereal supplement during early lactation on the performance and intake of ewes grazing perennial ryegrass at three stocking rates

A comparison was made of the effect of a cereal supplement during early lactation on the performance and intake of ewes suckling two lambs while rotationally grazing pasture at stocking rates of 20, 17 or 14 ewes per ha. Supplement (S) at the high (H) and medium (M) stocking rates resulted in higher organic matter (OM) intakes (grass + supplement) of 2040 and 2500 g d^-1 respectively, compared with the herbage OM intakes of unsupplemented (U) ewes of 1750 and 2040 g d^-1 respectively. At the low (L) stocking rate the total OM intake of supplemented ewes and the herbage OM intake of unsupplemented ewes was similar at 2250 and 2210 g d^-1 respectively.
Lamb growth rate (g d^-1) to 12 weeks of age was significantly lower on treatment UH (229) compared with those on UM (253) and UL (262). Growth rates on S treatments were similar (SH 248; SM 261; SL 272) and there were no significant differences between S and U within stocking rates. Unsupplemented ewes lost significantly more weight during the period of intake measurement than those receiving supplement. The speed of rotation was faster where supplement was not fed at the medium and high stocking rates and herbage accumulation under UH was less than under SH. Only at the high stocking rate did feeding a supplement give a higher financial output.     

A Comparison of Three Techniques for Closure of Pelvic Flexure Enterotomies in Normal Equine Colon

Six enterotomies 3 cm long, spaced at 3 cm intervals, were made in the antimesenteric border of the pelvic flexure of the ascending colon in five adult horses. Ten incisions each were sutured with a Utrecht pattern (single layer), a full-thickness simple continuous oversewn with a Cushing pattern (two layer), and a mucosal simple continuous with a seromuscular simple continuous oversewn with a Cushing pattern (three layer). In all horses, chromic gut and polyglactin 910 were each used once for each pattern. On day 6, the pelvic flexure was excised and the colonic vessels were injected with a radio-opaque mass. Sections of each enterotomy were studied by light microscopy and high detail radiography. The single-layer closure led to serosa-to-mucosa healing and a thin bowel wall, and the three-layer closure resulted in distortion of the bowel wall by hematoma formation and submucosal contamination with ingesta. The two-layer closure resulted in good alignment and improved healing. There was less inflammatory reaction with polyglactin 910 than with chromic gut.     

A constant potential titration method for studying the kinetics of Cu2+ desorption from soil and clay minerals

The kinetics of Cu²⁺ desorption from a contaminated Malaysian padi soil, English Supreme kaolinite and Wyoming bentonite are studied using a constant potential titration method. This technique involves maintaining a constant Cu²⁺-sensitive electrode potential corresponding to a Cu²⁺ ion activity below that which would naturally be supported by the soil in suspension. This is achieved by the controlled addition of complexing agent. Desorption occurs in response to the artificially reduced solution activity and the rate of the desorption reaction is revealed by the rate of addition of the complexing agent.
The desorption results were fitted to three established kinetic equations, all of which showed reasonable agreement with the data. In particular, a two-constant rate equation demonstrated some consistency between different materials. Both first-order and 'diffusion' kinetic equations appeared to apply within specific titration conditions. Each equation is discussed in terms of its implied desorption mechanism and the inconclusive nature of such mechanisms for short term (< 1 hour) desorption reactions is illustrated.     

A Wood Famine? The Question of Deforestation in Old Regime France

Both contemporaries and modern students have differed as towhether or to what extent France confronted a wood shortageduring the old regime. Certainly demands on French forests weregreat, with the royal government (especially the navy), industrialists,urban centres, and the peasantry all laying claim to woodlandresources. By the 1660s, Versailles attempted to regulate thesedemands; set priorities among them, giving highest priorityto the fleet; and establish sound conservation practices. Despite these efforts, French forests declined in both extentand quality because of a venal forestry service, the need toraise ready cash for the treasury, efforts to clear land forgrowing cereals, the obstruction of conservation efforts bylocal authorities, the unregulated grazing of livestock in woodlands,and expanding industrial fuel consumption. Not all consumerswere equally affected by this deterioration, however. Whilemaritime ship building confronted grave problems and peasantwood users suffered to some extent, cities and towns faced relativelyminor problems, and manufacturers such as iron and glass makersscarcely had to deal with a wood famine at all. The ample supply of wood militated against any shift to coalburning, especially among industrialists. As a dearth of firewoodand a consequent use of coal had been an important stimulusto the British economy in the seventeenth and eighteenth centuries,the ready availability of traditional fuels (wood and charcoal)may be considered as one factor that tended to retard Frencheconomic development.     

Evidence for new sources of NOX in the lower atmosphere

Laboratory studies show that the reaction of short-lived O2(B3Sigmau) molecules (lifetime approximately 10 picoseconds) with N2 and the photodissociation of the N2:O2 dimer produce NOx in the stratosphere at a rate comparable to the oxidation of N2O by O(1D). This finding implies the existence of unidentified NOX sinks in the stratosphere. The NO2 observed in this experiment is isotopically heavy with a large 15N/14N enhancement. However, photodissociation of this NO2 unexpectedly produced NO molecules with a low 15N/14N ratio. The diurnal odd-nitrogen cycle in the stratosphere will be marked by a complex isotope signature that will be imprinted on the halogen and HOX catalytic cycles.     

Effect of soil compaction by tractor traffic on soil structure, denitrification, and yield of wheat (Triticum aestivum L.)

In a field experiment with soil compaction by tractor traffic on a loam soil, the denitrification rate (using the C₂H₂ inhibition method), the soil structure, and the wheat yield were investigated. Tractor traffic on wet soil (> – 50 mbar matric potential) reduced the pore volume, doubled the percentage of large aggregates (> 20 mm), reduced the wheat yield by about 25%, and increased the N-loss through denitrification by a factor of 3–4. Neither of these parameters were affected by tractor traffic at low soil moisture content. The weight of the tractor (1800 kg vs 4800 kg) did not significantly alter the effect of compaction on the measured parameters. There was a factor of 2–6 between the measured denitrification rate in compacted and that in uncompacted soil, and this factor showed little dependence on the average activity level on each date of measurement. Accumulated values for the measured denitrification during 75 days (May 23-August 9) were 3–5 kg N ha^–1 in uncompacted soil and 15–20 kg N ha^–1 in soil which was compacted in wet condition.