Effect of atorvastatin on neointimal hyperplasia of venous autografts in rats

AIM: To investigate the effect of atorvastatin on neointimal hyperplasia of autogenous vein graft in rats. METHODS: The model of autogenous vein graft was prepared by transplanting the external jugular vein into aorta in Wistar rats. The rats were divided into three groups: sham operation group, graft control group and graft experimental group. From three days after transplantation, the rats of autograft experimental group were treated by atorvastatin at a dosage of 5 mg·kg-1·d-1. Four weeks after treatment, venous autografts were removed at autopsy and cut into 4 μm sections. Histopathological examination was carried out to analysis the neointimal hyperplasia of grafted veins. Immunohistochemical staining was conducted to evaluate SMα-actin and PCNA expression of neointimal cells in venous autografts. RESULTS: In venous autograft control and experimental groups, SMα-actin-positive smooth muscle cells were proliferated and accumulated excessively in venous autografts, which resulted in significant neointimal formation and vascular lumen narrowing. Neointima quantitative assay revealed that the neointimal hyperplasia of venous autografts was suppressed obviously in graft experimental group, and its neointimal area and NIA/MA ratio of venous autografts were significantly lower than those in graft control group (P<0.01). Immunohistochemical assay indicated that the PCNA labeling index of neointimal cells was significantly lower in graft experimental group than that in graft control group (P<0.01). CONCLUSION: Atorvastatin significantly inhibits the proliferation of neointimal smooth muscle cells and the development of neointimal hyperplasia of venous autografts in rats. Atorvastatin is a powerful inhibitor of restenosis after vascular reconstructive operation with a potential for therapeutic use.     

小果型西瓜新品种‘圣女红2号’

 ‘圣女红2号’为优质、特早熟、小果型西瓜新品种。植株长势强健, 抗病抗逆性强, 雌花出现早, 易坐果, 果实膨大快, 单果质量1.8～2.2 kg。果实椭圆形, 浅绿底覆墨绿细条带, 果肉浓粉红色, 肉质松脆细嫩, 品质佳, 适合各地春播大中棚特早熟覆盖栽培。     

富士苹果AFLP体系的优化及其在鉴定早熟芽变中的应用

 以‘长富2号’苹果为材料,采用优化的AFLP技术对其早熟芽变与母株进行初步研究,筛选了64对引物,其中43对引物扩增结果理想,共产生了2,700条带。有4对引物在芽变与母株之间产生了7条差异片段,主要集中在350~800 bp之间。研究表明早熟芽变是由于遗传物质改变造成的,同时也说明利用该体系对苹果芽变进行分析和鉴定获得成功。     

北京市小龙门国家森林公园大型真菌名录

北京市小龙门国家森林公园大型真菌资源丰富。在保护区内共采集标本212号,鉴定出47种,隶属于2个门、7个目、20个科、47个种。     

三疣梭子蟹基因组微卫星特征分析

对三疣梭子蟹(Portunus trituberculatus)部分基因组DNA文库测序,获得了总长度为622409个碱基的基因组DNA序列,从中找到微卫星重复序列(1～6bp重复)697个.统计微卫星重复类型,以两碱基重复数目最多,为445个,占微卫星序列总数目的63.84%;其次是三碱基重复152个,占21.81%;再次分别是单碱基重复45个,占6.46%;四碱基重复31个,占4.45%;五碱基重复14个,占2.01%;六碱基重复1个,占1.43%.在单碱基重复类型中,重复拷贝类别全部为A:两碱基重复类型中,AG重复数目最多,其次是AC和AT;三碱基重复类型中以ACT最多,其次是AGG和AAT;四碱基重复类型中,AGAC重复数目最多;五碱基重复类型中,以AACCT重复拷贝类别最多;六碱基重复中以AGGGGA重复数目最多.GC重复拷贝类别的重复数目很少,只发现1个(GenBank注册号为EU113241).     

寒地梨种质资源表型多样性研究

对寒地梨种质资源表型性状的Simpson和Shannon多样性指数、标准差、变异系数和聚类分析等研究表明幼叶颜色、叶面状态、果实形状、抗寒性、抗梨黑星病、单果质量、可溶性固形物等性状多样性指数较高,变异度大,可为梨育种提供良好变异的群体;尤其是保存了多份极抗寒资源,抗寒性中等资源较少,无抗寒性弱和极弱的资源;果实形状多样性较高,而且性状间分布也较为均匀,可溶性固形物变异系数较大;大果型、抗梨黑星病类型资源分布较少,尚需收集、增加果个大、高抗病资源。     

流域梯级水电站实时水情预报管理系统研究

综合运用基于DEM的流域水系模型与TOPMODEL水文模型构建出了流域梯级水电站实时水文预报模型,并以VS.Net为平台,运用分布式网络编程技术将二者集成,为西南某流域梯级水电站设计开发出功能先进和完善的网络版实时水情预报管理软件系统.该系统已投入使用,为实现流域水情预报的智能化、信息化和网络化管理提供了可靠的辅助工具.     

赴澳大利亚节水灌溉技术学习考察启示

在澳期间通过听专家专业授课、专题访问、参观、调查等活动,学习考察了澳大利亚关于水资源管理与利用、节水措施、土壤改良技术应用、风力提水技术应用、集雨技术应用、覆盖技术应用、棉花滴灌技术应用、滴灌系统过滤装置等方面应用管理情况.     

基于AT89S51的汽车智能安全报警系统

利用AT89S51设计了一种新型汽车防盗报警系统.当满足报警条件,单片机会向GSM模块发出指令,系统向机主终端发出报警求助短信,同时还发出声光报警、切断发动机电源.本系统具有误报率小,可靠性高,成本低,使用方便等优点.     

外源植酸酶提高土壤磷素有效性的研究

土壤有机磷对土壤肥力和植物营养有重要的影响,但只有在水解转化为无机磷后才能被植物吸收利用,必须提高土壤磷素的有效性.植酸酶是一类特殊的酸性磷酸酶,被广泛应用于饲料中的添加剂并引入土壤,以研究其对土壤磷酸酶活性和有机磷转化的影响,进而提高土壤磷素有效性.