动物家谱分析系统的研制与开发

本文论述了动物家族系谱分析系统的组成及其功能，采用线条图的形式来描绘动物各代之间的血缘关系，通过对动物个体在家族中的特性作用的统计分析，将为动物养殖繁育提供重要的参考价值。     

建设西藏林芝鲁郎森林浴场的探讨

建设西藏林芝鲁郎森林浴场,既增加鲁郎林海新的利用途径,又能满足人们渴望休闲保健的需求。西藏林芝鲁朗森林资源环境优越,具有开发森林浴场的条件。在探讨西藏鲁朗森林浴场的选址和项目设施建设方面,应遵循一定的原则,综合考虑森林浴的保健、休闲旅游功能。     

试论少儿图书馆如何开展阅读指导工作

阅读是教育的核心.是人类必不可少的一种能力.少儿图书馆在指导少儿阅读、开展素质教育方面起着不可替代的作用.从几个方面简要论述了如何有效地开展少年儿童阅读指导工作.     

麦糟固态发酵生产纤维素酶

以麦糟为主要原料 ,利用里氏木霉 (TrichodermareeseiRutC 30 )固态发酵生产纤维素酶 ,在浅盘发酵中 ,通过对发酶过程品温和曲料pH的优化控制 ,得到了优化后的工艺条件。在发酵前期品温控制在 2 7～ 30℃ ,后期最高不超过 35℃ ,最高酶活达到 611U/g曲。当曲料pH第一次回升至 4 .3～ 4 .5时 ,用稀盐酸进行喷酸控制。采用优化后的pH和品温控制工艺 ,FPA酶活达到 50 8U/g曲 ,相当于 2 12 0U/g纤维素     

无菌稀释液对禽疫苗免疫效果的影响

无菌稀释液在养殖业发达的国家已成为畜禽防疫的必备产品.黑龙江省生物制品一厂为了进一步改进无菌稀释液产品质量,在原有产品基础上加入免疫促进剂,并对采用本品稀释的Lasota和法氏囊炎疫苗的免疫效果进行了试验观察.     

颗粒剂农药防治辣椒疫病的研究

选择了５种杀菌剂，将其制成具有缓释性能的颗粒剂，施在辣椒根茎基部防治辣椒疫病。室内接种试验，有４种颗粒剂施药７ｄ后防效１００％，２１ｄ后防效９５％以上。田间试验防效平均在８４％以上，连续３年田间应用，防效为８０％左右。     

ST14/MT-SP1 influences expression of MT2-MMP and TIMP-2 to promote invasiveness of colorectal cancer cells

AIM: To evaluate how ST14/MT-SP1 overexpression alters invasiveness of colorectal cancer cells.METHODS: Human full length ST14/MT-SP1 gene was transiently transfected into colorectal cancer (RKO) cell lines.The expression products were purified by chromatography on Ni2+-affinity resin column and analyzed for gelatinase activity by gelatin zymography.Cell invasion and migration were determined by ECM invasion assay in vitro.RNA was isolated from stable ST14-transfected RKO cells and a cDNA microarray was utilized to detect the change in expression of MMPs and TIMPs.Real-time quantitative PCR was used to validate the change of expression.RESULTS: The full length ST14/MT-SP1 was transfected and expressed in RKO cells.The expressed protein showed a gelatinase activity.In addition,invasiveness of RKO was significantly enhanced by ST14/MT-SP1 overexpression (P<0.01).Blockage of ST14/MT-SP1 resulted in a decrease in the invasiveness of SW480 and SW620 (P<0.01).Furthermore,MT2-MMP (MMP-15) expression was significantly up-regulated (2.5-fold change) and TIMP2 down-regulated (0.35-fold change) by ST14/MT-SP1 overexpression in RKO.CONCLUSION: ST14/MT-SP1 overexpression results in an increase in invasiveness of colorectal cancer RKO cells.Increased invasiveness is due to an increase in the gelatinase activity of ST14/MT-SP1 and a change in up-regulated MT2-MMP and down-regulated TIMP-2 expression.Therefore,ST14/MT-SP1 overexpression enhances invasiveness of colorectal cancer cells.     

浅谈新形势下高校的辅导员在思想政治工作中的角色转变

分析了当前中国高校的思想政治教育形势,辅导员面临的挑战,以及作为高校思想政治教育主要执行者的思想政治辅导员角色该如何转变。     

Expression of tissue factor,tissue factor pathway inhibitor in the atherosclerotic plaque on femoral artery of New Zealand rabbits

AIM: To observe the regulatory changes of tissue factor (TF),tissue factor pathway inhibitor-1 (TFPI-1) and tissue factor pathway inhibitor-2 (TFPI-2) associated with the formation of atherosclerotic plaque on femoral artery of New Zealand rabbits.METHODS: New Zealand rabbits were fed with high cholesterol diet and received femoral artery balloon injury to establish moderated atherosclerotic plaque animal model.These rabbits were classified into 8 weeks group,10 weeks group and 12 weeks group.The expressions of factor TF,TFPI-1 and TFPI-2 in femoral artery were detected by immunohistochemical methods at 8th,10th and 12th week.The positive areas of the slice were analyzed by semi-quantification system.Fluorescence quantification PCR (FQ-PCR) was used to detect the copies of mRNA of TF,TFPI-1 and TFPI-2 in each group.RESULTS: The expressions of TF,TFPI-1 and TFPI-2 determined by immunohistochemical methods were increased from 8th,10th to 12th week,respectively.In each group,the quantity of mRNA of TF and TFPI-1 at the 8th,10th and 12th week was soared,respectively.In each group,the quantity of mRNA of TFPI-2 did not change in early phase,but decreased at 12th week.CONCLUSION: In the process of the atherosclerotic plaque formation in rabbit femoral artery model,the expressions of TFPI-1 and TFPI-2 proteins are increased.However,the expression of TPFI-1 or TFPI-2 could not stave off the development of plaque formation.