Acute and sublethal toxicity of novaluron, a novel chitin synthesis inhibitor, to Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

The acute and sublethal toxicities of novaluron, a novel chitin synthesis inhibitor, to a laboratory-reared insecticide-susceptible strain of Colorado potato beetle, Leptinotarsa decemlineata (Say), were determined. Novaluron exhibited excellent residual (120 h LC(50) = 0.42 mg litre(-1)) and good direct contact (120 h LC(50) = 27 mg litre(-1)) activity against second-instar larvae (L2). Hatch of eggs exposed by direct contact to novaluron solutions > or =100 mg litre(-1) was significantly reduced, as was the ability of emerged first-instar larvae to moult. L2 from eggs exposed to > or =100 mg litre(-1) novaluron weighed significantly less (P < 0.0001) than those from untreated eggs. However, L2 from eggs treated with 1 mg litre(-1) novaluron weighed significantly more (P < or = 0.05) than those from untreated eggs, suggesting novaluron can have a hormetic effect on L decemlineata larval development. Leptinotarsa decemlineata mating pairs fed foliage treated with novaluron at 25 or 75 g AI ha(-1) produced approximately 25% fewer egg masses and eggs per mass. Hatch of eggs on treated foliage was almost completely suppressed, and longevity of male beetles was reduced by approximately 50% when fed foliage treated with novaluron at 75 g AI ha(-1).     

Perceptions of risk, risk aversion, and barriers to adoption of decision support systems and integrated pest management: an introduction

Rational management of plant diseases, both economically and environmentally, involves assessing risks and the costs associated with both correct and incorrect tactical management decisions to determine when control measures are warranted. Decision support systems can help to inform users of plant disease risk and thus assist in accurately targeting events critical for management. However, in many instances adoption of these systems for use in routine disease management has been perceived as slow. The under-utilization of some decision support systems is likely due to both technical and perception constraints that have not been addressed adequately during development and implementation phases. Growers' perceptions of risk and their aversion to these perceived risks can be reasons for the "slow" uptake of decision support systems and, more broadly, integrated pest management (IPM). Decision theory provides some tools that may assist in quantifying and incorporating subjective and/or measured probabilities of disease occurrence or crop loss into decision support systems. Incorporation of subjective probabilities into IPM recommendations may be one means to reduce grower uncertainty and improve trust of these systems because management recommendations could be explicitly informed by growers' perceptions of risk and economic utility. Ultimately though, we suggest that an appropriate measure of the value and impact of decision support systems is grower education that enables more skillful and informed management decisions independent of consultation of the support tool outputs.     

Metabolism, uptake and translocation of 14C-paraquat in resistant and susceptible biotypes of Crassocephalum crepidioides (Benth.) S. Moore

The metabolism, uptake and translocation of paraquat in resistant (R) and susceptible (S) biotypes of Crassocephalum crepidioides (Benth.) S. Moore (redflower ragleaf) at the 10-leaf stage was investigated. A study on the properties of leaf surface was carried out to examine the relationship between leaf surface characters and paraquat absorption. The extractable paraquat was not metabolized by the leaf tissue of either the resistant or susceptible biotypes. Differential metabolism, therefore, does not appear to play a role in the mechanism of resistance. Both biotypes did not show any significant difference in the amount of cuticle and trichome densities. Furthermore, both biotypes are identical in the structure of stomata, trichomes and epicuticular wax. The results of the leaf surface studies are in agreement with the findings of the uptake study. Both biotypes demonstrated no significant difference in absorption between the resistant and susceptible biotypes. However, 10% of the absorbed ¹⁴C-paraquat into the S biotype was translocated basipetally, but not in the R biotype. The results of this study suggest that in C. crepidioides , differential translocation may contribute to the mechanism of resistance at the 10-leaf stage.     

Interference between triazine-resistant Brassica napus and Panicum miliaceum

Atrazine carryover often limits growers to production of atrazine-tolerant crops the year following application, and allows the increase of triazine-tolerant weed species such as Panicum miliaceum L. (wild proso millet). Tiriazine-resistant Brassica napus L. cv. ‘Triton’ (oilseed rape) was tested to characterize the nature of interspecific interference with P. miliaceum. In a greenhouse study, atrazine at 2.2 kg ha^?1 depressed oilseed rape fruit (siliqua) number and fruit dry weight, and delayed flowering, but did not significantly affect height or weight of shoots, Oilseed rape fruit weight was reduced at 200 P. miliaceum plants m^?2. fruit number and shoot weight were inhibited at 400 weeds m^?2. and height was reduced and flowering delayed at 600 weeds m^?2. Number and weight of fruits were reduced by one-third after 8 weeks of interference as compared to oilseed rape grown with the weed for 4 weeks. Oilseed rape height was reduced by 29% and shoot weight by 55% by 600 weeds m^?2 and 2–2 kg ha^?1 atrazine, while fruit number and weight were reduced by 72%. Oilseed rape shoot weight was reduced by 74% by 600 weeds m^?2 for 12 weeks of interference, while fruit number and weight were reduced by 85% and 82%. respectively. In a field study, fluazifop reduced early season P. miliaceum cover by 72%, but did not increase oilseed rape cover. Mid-season P. miliaceum shoot weight was decreased by 97% by fluazifop and oilseed rape shoot weight was increased by 34%. P. miliaceum control increased oilseed rape biomass by 38% at 89 days, but biomass of oilseed rape sown at 11.2 kg ha^?1 with 2.2 kg atrazine ha^?1 was not decreased by P. miliaceum interference at 89 days.     

Characterization of cereal bare patch isolates of Rhizoctonia solani by random amplified polymorphic DNA analysis

RAPD-PCR was used to characterize isolates of Rhizoctonia solani from bare patches in cereal and pasture crops at two locations in Western Australia, Newdegate and Esperance. All of the isolates belonged to anastomosis group 8, pectic zymogram group 1-1 or 2. The pectic zymogram assignment could be confirmed by RAPD-PCR. There was no difference in RAPD-PCR pattern between highly virulent and weakly virulent isolates at Newdegate, or between isolates from different patches at Newdegate. The Newdegate isolates were identical to isolates from Esperance, and to isolates from various locations in South Australia.     

Resistance of Erysiphe graminis on barley and wheat to sterol C-14-demethylation inhibitors1

Isolates of Erysiphe graminis f. sp. hordei and tritici with decreased sensitivity to triadimefon showed cross-resistance to other inhibitors of sterol C-14-demethylation, such as triadimenol, propiconazol, diclobutrazol, prochloraz and nuarimol. The isolates exhibited a moderate degree of resistance to these compounds. No cross-resistance was detected to tridemorph, fenpropimorph and pyrazophos. The resistant hordei isolates were more sensitive to ethirimol than the sensitive isolate. The competitive abilities of resistant hordei and tritici isolates were inferior to that of the sensitive isolates. In the presence of the fungicides no differences in germination, appressorium formation and penetration between the sensitive and resistant isolates were observed; 48 h after inoculation the sensitive isolate showed several morphological alterations and further fungal development was arrested. At four to five times higher doses of triadimefon, similar morphological alterations were detected in the resistant isolate. Low concentrations of triazole fungicides which slightly affected mycelium growth of both the sensitive and the resistant isolate of f.sp. hordei severely inhibited development of conidiophores of the sensitive isolate whereas that of the resistant isolate was hardly affected.