SMAS平面下面颈皮瓣修复颊部皮肤缺损

目的报道SMAS平面下面颈皮瓣修复颊部皮肤软组织缺损的修复方法和疗效。方法于创面邻近部位设计前蒂或后蒂面颈皮瓣,于SMAS平面下掀起皮瓣后向内侧旋转推进覆盖颊区皮肤软组织缺损共20例。结果20例皮瓣均全部成活,其中1例术后腮腺瘘形成。术后随访2~13月,皮瓣外观满意,皮肤浅感觉良好,除1例术后半年内下睑外翻外余无面部器官移位及面神经损伤表现。结论SMAS下面颈皮瓣血供可靠,色泽、质地优良,与颊部匹配,是修复颊部皮肤软组织缺损的理想组织瓣。     

县域农业可持续发展的生态安全评价——以吉林省通榆县为例

以吉林省通榆县为例,构建了县域农业可持续发展的生态安全评价指标体系,采用均方差法确定了评价指标的权重,对研究区1985、1990、1995、1998、2001、2004年6个时段的农业生态安全进行了评价.从评价结果可以看出,近20年,通榆县农业生态安全呈现下降态势,安全等级处于Ⅲ～Ⅴ级,安全状态从敏感态下降到风险态.1998年是研究区农业生态安全变化的转折点,从1998年起,农业生态安全有所回升.在分析变化原因的基础上,提出农业可持续发展的生态安全建设对策.     

辽西地区相对资源承载力与可持续发展研究

本文讨论并改进了相对资源承载力的概念、研究思路和计算方法,选择具有代表性的辽西地区,实证分析1988—2004年辽西地区相对土地资源承载力、相对经济资源承载力、相对生活水平承载力和综合承载力的演变和空间差异,预测2010年和2015年辽西地区的人口超载规模,分析辽西地区相对资源承载力存在问题的原因,提出辽西地区实现可持续发展的对策。     

磁处理水对两种十字花科植物种子发芽生长的影响

为了观察磁处理水培养液对2种十字花科植物莱菔与白芥种子发芽生长的影响．使用不同强度的磁处理水培养液对莱菔种子和白芥种子进行处理，设置对照组，7d统计发芽率、根长、根重，测相对电导率。结果表明：0．08T、0．10T强度磁处理水培养液处理的莱菔子与白芥子发芽和根生长情况均好于对照组（P〈O．05）．0．08T、0．10T磁处理水培养液处理的两种幼株的电导率均低于对照组。说明磁处理水培养液对两种十字花科植物莱菔与白芥种子发芽生长有影响，其生长情况与磁场强度有关。     

早中熟棉花品种——新陆中51号

新疆是棉花的主产区,其中南疆产量约占新疆的70％,全国的20％.新陆中51号(K215)是新疆石大科技公司、石河子大学棉花所和巴州一品种业公司共同选育的早中熟高产陆地棉新品种,具有优质、高产、抗病、适应性强等特性.2011年8月经农业部、新疆维吾尔自治区农作物品种审定委员会审定、命名(新农审2011年46号).新陆中51号的选育及推广应用,将为南疆棉花增产增收和发展优质棉提供优良品种支持.     

益生酵母在滩羊日粮中适宜添加量的研究

在精粗比为3∶7的滩羊日粮中,添加不同剂量的益生酵母(0,0.1%,0.3%,0.5%和0.7%)进行了体外批次培养,并应用单项组合效应值(SFAEI)和多项组合效应指数(MFAEI)对中性洗涤纤维(NDF)含量、氨氮(NH3-N)浓度、菌体蛋白(BCP)浓度、pH、产气量和总挥发性脂肪酸(VFA)浓度进行评定。单项组合效应值评定结果表明:NDF含量以添加量为0.1%组高于其他添加组,NH3-N浓度以0.3%组较高,BCP浓度以0.1%和0.3%组较高,pH以0.3%和0.5%组较高,产气量和总VFA浓度均以0.3%和0.7%组较高。多项指标综合评定结果表明:在精粗比为3∶7的日粮条件下,益生酵母在滩羊日粮中的适宜添加量为0.3%。     

饲粮能量和蛋白质水平对滩羊小肠中小肽和氨基酸转运载体mRNA表达量的影响

本试验旨在研究饲粮能量和蛋白质水平对滩羊小肠中小肽和氨基酸转运载体mRNA表达量的影响。选取112只健康、体重相近的滩羊,随机分成4组,每组4个重复,每个重复7只羊。标准水平的饲粮能量和蛋白质水平参考《肉羊饲养标准》(NY/T 816—2004),各组试验滩羊分别饲喂不同能量和蛋白质水平饲粮:0.84×标准水平(Ⅰ组)、0.96×标准水平(Ⅱ组)、1.08×标准水平(Ⅲ组)和1.20×标准水平(Ⅳ组)。试验根据羊体重分2个阶段:29~35 kg和36~40 kg。于每个阶段末,每个重复屠宰1只试验羊,取其小肠组织样,运用实时荧光定量PCR技术,研究小肽转运载体1(Pep T1)、y+型氨基酸转运载体1(CAT1)、兴奋性氨基酸转运载体3(EAAT3)mRNA表达量的变化。结果表明:1)在29~35 kg阶段末,小肠中Pep T1 mRNA的表达量随着饲粮能量和蛋白质水平的提高呈先下降再上升的趋势,Ⅱ组显著低于其他3组(P0.05);Ⅳ组小肠中CAT1 mRNA的表达量显著高于其他3组(P0.05);Ⅲ组小肠中EAAT3mRNA的表达量显著高于其他3组(P0.05)。2)在36~40 kg阶段末,Ⅱ组小肠中Pep T1mRNA的表达量显著高于其他3组(P0.05);Ⅱ组小肠中CAT1 mRNA的表达量显著高于Ⅲ组(P0.05);小肠中EAAT3 mRNA的表达量随着饲粮能量和蛋白质水平的提高呈上升趋势,Ⅲ组和Ⅳ组小肠中EAAT3 mRNA的表达量显著高于Ⅰ组和Ⅱ组(P0.05)。由此可见,饲粮能量和蛋白质水平会影响滩羊小肠中Pep T1、CAT1、EAAT3 mRNA的表达量,使机体对小肽和氨基酸的吸收利用率随之改变,以适应滩羊的生长发育。     

Comparison of 2 methods for establishing rat model of chronic osmotic diarrhea

AIM: To compare 2 different rat models of chronic osmotic diarrhea with the lactose enrichment and magnesium citrate methods. METHODS: The SD rats (n=31) were randomly divided into lactose enrichment diarrhea group (Lac group, 12 rats), magnesium citrate diarrhea group (Mg group, 12 rats) and normal control group (Con group, 7 rats). The rats in Lac group were fed with a lactose enrichment diet and freely drank water. The rats in Mg group were fed with a standard diet and drank magnesium citrate solution. The Con group was fed with a standard diet and normal water. The general conditions and the characteristics of stool were observed every day. The grip strength and motor activity of the rats were measured after diarrhea for 14 d. The trans-epithelial resistance of the colonic mucosa was detected by Ussing chamber. The pathological changes of villus height and villus surface area in the intestine were observed with hemato-xylin-eosin staining. RESULTS: The rats in both diarrhea groups displayed the clinical manifestations of slow increase in body weight, spare and dull hair, lacking in strength and physical inactivity. The grip strength and motor activity of the rats in both diarrhea groups decreased significantly compared with Con group. The diarrhea rate and diarrhea index of the rats in the 2 diarrhea groups were both significantly higher than those in Con group (P<0.05). Moreover, the diarrhea index in Lac group was significantly increased compared with Mg group. The thickness of intestinal mucosa in Lac group was thinner than that in Mg group at the same time. However, the damage of intestinal villi in Mg group was more serious than that in Lac group.CONCLUSION: Both methods of lactose enrichment-induced diarrhea and magnesium citrate-induced diarrhea meet the requirement of chronic osmotic diarrhea, but there are different advantages between the diarrhea models. Therefore, we should select the correct diarrhea model to meet different aims of the experiments.     

国审稻新品种松辽838选育技术报告

为育成适宜北方稻区种植的高产稳产、丰产性好、抗逆性强、品质优良、适应性广等综合性状优异的中早粳早熟品种,促进北方稻区水稻生产的发展,开展此水稻遗传育种课题。叙述水稻新品种松辽838(松辽11-838)的选育过程及参加国家3 a试验的生育表现与试验结果,以期使广大农民更好地了解品种的综合性状和栽培技术,应用生产后,发挥更大的品种增产作用。     

Effects of Dietary DHA/EPA Ratios on Fatty Acid Composition,Lipid Metabolism‐related Enzyme Activity,and Gene Expression of Juvenile Grass Carp,Ctenopharyngodon idellus

To determine the effects of docosahexaenoic acid/eicosapentaenoic acid (DHA/EPA) ratios on grass carp, Ctenopharyngodon idellus, a 38‐d feeding trial was conducted using six isonitrogenous and isoenergetic semi‐purified diets containing constant n‐3 long‐chain polyunsaturated fatty acid (LC‐PUFA) (0.5% of dry matter), but varying ratios of DHA to EPA and a control diet (no n‐3 LC‐PUFA was included). The results revealed higher final weight and specific growth rate in the DHA/EPA 0.21 group. The n‐3 LC‐PUFA content increased in the CK (control) groups compared with that in the control diet. Lipoprotein lipase (LPL) activity increased in the treatment groups. Malate dehydrogenase showed lower activity in the DHA/EPA 1.08 group, as well as to the change in the level of glucose‐6‐phosphate dehydrogenase (G6PDH). The gene expressions of LPL increased in the treatment groups and that of peroxisome proliferator‐activated receptor α gene showed higher expressions in DHA/EPA 1.08, 0.49, and 0.21 groups. However, no remarkable differences were found among the six groups in the peroxisome proliferator‐activated receptor γ gene expression. Our findings indicated that dietary n‐3 LC‐PUFA affected fatty acid composition and lipid metabolism of grass carp. Further, fish achieved the best effect in decreasing the lipid accumulation when dietary DHA/EPA ratio was not greater than 1.