Tissue distribution of bovine spongiform encephalopathy infectivity in Romney sheep up to the onset of clinical disease after oral challenge

Sixty Romney sheep of three prion protein genotypes were dosed orally at six months of age with an inoculum prepared from the brains of cattle clinically affected with BSE, and 15 sheep were left undosed as controls. They were randomly assigned within genotype to groups and were sequentially euthanased and examined postmortem at intervals of six or 12 months, depending on their predicted susceptibility. Tissue pools prepared from the three, four or five dosed animals in each group were inoculated into groups of 20 RIII mice as a bioassay for infectivity. Separate inocula were prepared from the matched control sheep killed at each time. In the ARQ/ARQ sheep killed four months after inoculation, infectivity was detected in the Peyer's patch tissue pool, and at 10 months it was detected in the spleen pool; from 16 months, infectivity was detected in a range of nervous and lymphoreticular tissues, including the spinal cord pool, distal ileum excluding Peyer's patches, liver, Peyer's patches, mesenteric and prescapular lymph nodes, spleen, tonsil and cervical thymus. No infectivity was detected in the tissue pools from the ARQ/ARR and ARR/ARR sheep killed 10 months or 22 months after infection.     

家鱼人工繁殖技术

家鱼人工繁殖技术袁大林家鱼系指青、草、鲢、鳙鱼，根据从事多年家鱼人工繁殖生产者的经验，谈谈家鱼人工繁殖技术。一、带鱼收集亲鱼好否，对今后的催产、孵化以及鱼苗的成活率，生长速度都有很大的影响，因此在选择亲鱼应尽量选择天然青、草、鲢、鳙原种，链鱼３－５龄...     

A modified bilateral transfrontal sinus approach to the canine frontal lobe and olfactory bulb: surgical technique and five cases

Five adult dogs presented for an acute onset of seizure activity. Magnetic resonance imaging revealed lesions in the olfactory bulbs, frontal lobes of the cerebrum, or both. A modified bilateral transfrontal sinus craniotomy was performed on each patient. The goal of removing the lesion was to relieve clinical signs and to provide tissue for histopathological diagnosis. In each instance, excision of the lesion was possible using this approach. No postoperative complications were observed. The modified bilateral transfrontal sinus craniotomy provides excellent access to the canine olfactory bulbs and frontal lobes.     

雉鸡育雏技术

雉鸡又名七彩山鸡、野鸡、环颈雉等,具有较高的观赏、食用、药用价值。发展雉鸡养殖是一条很好的致富门路。在雉鸡的培育过程中,育雏期尤为关键,这是因为雏雉对环境的适应能力差。因此,掌握育雏技术,科学饲养管理雏雉,能提高雏雉的成活率,促进生长发育,减少疾病发生,提高生产性     

科学免疫距离我们有多远?

免疫是猪场一项重要的日常工作,是保证猪场正常生产的有力措施,所以免疫对养猪人来说都非常熟悉。但就是这个非常普通的工作环节,却一直存在很多问题 。 猪病种类多,感染途径复杂,所以很多猪场使用疫苗十几种,小猪一出生就要各种疫苗的“辅佐”,并且跟随其一生,疫苗能有效地防止疫病发生,但是增加的应激也着实不少;因此产生一个极端反向观点,认为猪病太多了,我也不知道到底应该打哪些苗,如果都打,不但投资大,而且可能产生副作用,所以什么苗也不打;另外一部分猪场比较重视防疫,请兽医制订免疫程序,这其中有饲养管理各个方面措施到位的,生产平…     

小蚕人工饲料蚕品种“茗山×碧水”在会东县桑叶饲育调查

在凉山州会东县采用常规桑叶育方式饲育小蚕人工饲料蚕品种“茗山×碧水”,春季饲养成绩单产比“菁松×皓月”略低,比全县平均成绩高,丝质成绩相当;秋季饲育成绩和丝质成绩均显著好于对照种“781×7532”和全县平均成绩。     

蜂胶抗炎功效成分研究

为确定中国杨属型蜂胶的抗炎功效成分,用100ng/ml脂多糖(LPS)和40μg/ml氧化型低密度脂蛋白(ox-LDL)分别诱导脐静脉血管内皮细胞(HUVECs)损伤。通过SRB法分别检测蜂胶水提物中分离的11种组分和蜂胶乙酸乙酯提取物中分离的8段组分对2种炎症细胞模型细胞存活率的影响。结果表明:蜂胶水提物中的11种组分不同程度的促进2种炎症细胞的细胞存活率,乙酸乙酯提取物中的8段组分也是不同程度的提高2种炎症细胞的细胞存活率。由此可知,蜂胶的抗炎功效成分十分复杂,很可能是多种成分协同功效的结果,而且酚酸类物质发挥了一定的作用。     

普通鸡蛋、柴鸡蛋和乌鸡蛋中铜、铬、锰含量比较研究

为了研究普通鸡蛋、柴鸡蛋、乌鸡蛋蛋黄及全蛋中铜(Cu)、铬(Cr)、锰(Mn)元素的含量,试验选择生产和包装日期相近的普通鸡蛋、柴鸡蛋和乌鸡蛋各120枚,采用原子吸收光谱法测定鸡蛋样品中的Cu、Cr、Mn元素含量。结果表明:普通鸡蛋、柴鸡蛋、乌鸡蛋中均含有丰富的Cu、Cr、Mn元素,普通鸡蛋和柴鸡蛋蛋黄中的Cu元素含量显著高于乌鸡蛋(P0.05),普通鸡蛋蛋黄中的Cr元素含量显著高于柴鸡蛋和乌鸡蛋(P0.05),乌鸡蛋蛋黄中的Mn元素含量显著高于普通鸡蛋(P0.05)。说明在鸡蛋消费时可以根据对微量元素的不同需求进行选择。