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981.
Some aspects of the interaction of the bacterial parasite Pasteuria penetrans and the root-knot nematode ( Meloidogyne spp.) were investigated in laboratory and pot experiments. The variable spore attachment on juveniles exposed to water suspensions of the bacterium is probably attributed to differential susceptibility of biotypes within a heterogeneous Meloidogyne population. The relationship between spore concentration and attachment level is not linear over a range of spore dosages, indicating that even at very high spore concentrations the number of spores capable of attachment may not be present in excess and it is difficult to ensure sufficient numbers of spores to ensure infection will attach to all nematodes. Attempts to apply the bacterium in conditions such as might occur in seedbeds did not suppress nematode multiplication after transplanting in nematode-infested soil, indicating that the only effective application method is a thorough spore distribution in the planting sites. Two major constraints were revealed: high levels of spore attachment to juveniles does not always guarantee a significant reduction of egg laying and this is greatly influenced by the Meloidogyne biotype. Furthermore, the cumulative effect of the parasite in reducing Meloidogyne populations over several crop cycles was less than expected as the bacterium reduced intra-specific competition for the food supply and the less damaged root enabled many nematodes to survive. 相似文献
982.
983.
A high incidence (86%) of potyvirus infection was noted in tobacco plants exhibiting a form of leaf curl in South Africa. Despite leaf curl being reported in the literature to be of geminiviral aetiology, no geminiviruses were detected. Furthermore, no other virus particles were detected by virus purification, TEM and serology. Twelve species of dsRNA were consistently isolated from these tobacco plants, but were absent from other forms of leaf curl-affected and healthy tobacco. Aphid and mechanical inoculation demonstrated that the purified potyvirus(es) did not cause leaf curl symptoms, but rather mild mottle and mosaic symptoms in tobacco. Partial characterization of the potyvirus preparation showed a possible relationship to a South African strain of potato virus Y. Because potyvirus-inoculated plants did not manifest leaf curl symptoms, and because leaf curl symptoms were noted in some plants not infected with a potyvirus, it was concluded that the potyvirus is not involved in the leaf curl aetiology, but causes a latent infection, the symptoms of which are masked. The pattern of the dsRNA banding, induction of enations and lack of mechanical and seed transmission are common to plant reoviruses. The possibility of a phytoreovirus involvement in this form of leaf curl is currently being investigated. The results from this study suggest that tobacco leaf curl disease worldwide, with regard to geminiviruses, be re-evaluated. 相似文献
984.
The effect of soil moisture content on the suppression of Rhizoctonia stem canker on potato by mycophagous soil animals was studied in growth chambers. Three soil moisture levels were established in two bioassays, in which potato sprouts grew through a 15-cm soil layer inoculated with sclerotia of Rhizoctonia solani (AG-3). In one experiment two levels of R. solani inoculum were applied. The effect on plant disease of mycophagous soil fauna was assessed by adding the springtail Folsomia fimetaria and/or the nematode Aphelenchus avenae to the soil. In the absence of mycophagous organisms, Rhizoctonia disease severity on potato stems was highest in dry soil. A. avenae and F. fimetaria reduced Rhizoctonia stem canker when applied at populations found in the field. They were effective over a broad range of soil moistures. The stimulatory effect of dry soil conditions on Rhizoctonia stem canker was counteracted by a greater efficacy of the mycophagous soil fauna under these conditions. Mild drought stress did not seem to be a limiting factor in the biological control of stem canker by these two organisms. 相似文献
985.
Sunlight is known to reduce the incidence of blister blight disease of tea, caused by Exobasidium vexans . The effects of the ultraviolet-B (UV-B: 290–320 nm) component of solar radiation on the disease and the development of E. vexans were studied in the field in Sri Lanka using UV-screening filter materials held over a commercial crop. Exclusion of UV-B radiation by Polyester, which reduces fluxes in the UV-B region by approximately 75–85%, increased both the number of translucent spots (immature sites of infection) and number of sporulating blisters (mature sites of infection), but it had little or no effect on the sporulation of the pathogen. When basidiospores were artificially inoculated on leaves, and were exposed to full or filtered solar radiation, their survival and germination increased when UV-B wavelengths were removed; significant differences were found 62 h after inoculation. However, UV-B did not affect the extension of germ tubes. This study indicates that the UV-B component of solar radiation plays an important role in the natural regulation of blister blight disease in the field. 相似文献
986.
A model of the population dynamics of healthy weed plants, weed seeds in the soil, pathogen-infected weed plants and pathogen spores in the soil, was devised to investigate interactions that are important for the success of biocontrol with pathogens that prevent seed set. Three particular features of the host-pathogen interaction were examined in detail: the form of the density dependent relationship which determined seed and spore production, the host life stage at which infection could occur, and the relative competitive abilities of healthy and infected host plants. It was found that, when both weed and pathogen coexist, the equilibrium abundance of the weed in the presence of the pathogen was independent of the form of the relationship between plant density and seed or spore production. However, the form of this relationship did affect estimated equilibrium densities in the absence of biocontrol, and also affected the parametrizations under which both host and pathogen could coexist. Parameters derived from experiments with isolated host plants may therefore be sufficient to assess the biocontrol potential of new pathogens along with knowledge of densities achievable when the weed is uncontrolled. The form of the relationship used to control seed and spore production also had a marked influence on the range of parameter values over which the pathogen could persist. Other control measures were represented by changes to appropriate parameter values, e.g. weeding was represented by a change in weed death rate. With one exception, the use of additional control measures was not antagonistic to biocontrol. Often, however, the combined effect was less than additive, and the existence of synergy (where the combined effects are more than additive) was critically dependent on the form of the relationship of the rate of seed production per plant with density and the efficiency of the other control measures. 相似文献
987.
P. VIDHYASEKARAN R. RABINDRAN M. MUTHAMILAN K. NAYAR K. RAJAPPAN N. SUBRAMANIAN & K. VASUMATHI 《Plant pathology》1997,46(3):291-297
Pseudomonas fluorescens strain Pf1, inhibitory to the growth of the rice blast pathogen Pyricularia oryzae in vitro , was developed as a talc-based powder formulation. When rice seeds were treated with this formulation, the bacteria spread to roots, stems and leaves of the plants and protected against leaf infection by P. oryzae . When applied as a foliar spray, the bacteria survived on the leaves. The powder formulation controlled leaf blast under greenhouse conditions. In tests as a seed treatment and foliar spray in four field trials it effectively controlled the disease and increased grain yield. 相似文献
988.
Solarization trials were carried out over 3 years and in two countries to control crown gall disease on fruit trees and eliminate Agrobacterium . In 1992, agrobacteria in naturally infested soils of two Italian nurseries were monitored before and after solarization. Agrobacteria populations decreased by 99% and 92% after the treatment; however, crown gall incidence did not decrease. In 1993 and 1994 solarization was tested in Oregon in fields artificially infested with two marked strains of A. tumefaciens . In sandy loam soil, the target bacteria were eliminated in 4 weeks, while in silty clay soil the populations were markedly reduced after 2 months of treatment. Crown gall incidence on cherry rootstocks transplanted to the field at the end of 1993 was 3.7% in the sandy loam soil control plots, while no tumours were observed on plants from solarized plots. The use of solarization in combination with reduced doses of metham-sodium was also evaluated. 相似文献
989.
Airborne spores of the fungal pathogens causing Sigatoka diseases in banana and plantain were monitored using rotorod spore traps, sited at various heights within an infected plantation in Costa Rica from December 1993 to February 1994. Different capture patterns of ascospores and conidia were found and the relationship between wind behaviour and spore catches was investigated. This information has enabled an assessment to be made of the reliability of point measurements of airborne spores for monitoring spore movements on the plantation scale. The use of such information in forecasting the airborne movement of these spores and the likely role of the wind in the spread of this disease to uninfected areas is discussed. 相似文献
990.
A mathematical model was developed of the dynamics of a plant virus disease within a spatially-referenced lattice of fields of a host crop. The model can be applied to crops in continuous, contiguous cultivation such as tropical irrigated rice. Disease progress in each field of the host crop was assumed to be logistic and determined by incidence within the field itself as well as incidence in neighbouring fields, depending on the gradient of disease spread. The frequency distribution of planting dates (represented by the proportion of the total number of fields planted in successive months) was assumed to follow a normal distribution and the variance of planting date was used as a measure of cropping asynchrony. Analysis of the model revealed that disease incidence within the lattice (i.e. mean incidence over all fields) depended upon the infection efficiency, the slope of the dispersal gradient, and the variance in planting date. Disease endemicity depended mainly on planting date variance and disease persisted in the lattice if this variance exceeded a certain threshold. Above the threshold for persistence, the response of mean disease incidence to planting date variance was non-linear and the region of greatest sensitivity was closest to the threshold. Thus, disease systems that show moderate rather than high cropping asynchrony are more likely to be influenced by changes in the variance of planting date. Implications for the area-wide management of rice tungro virus disease are discussed. 相似文献