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991.
992.
Rory A. OShaughnessy Gregory G. Habing Wondwossen A. Gebreyes Andrew S. Bowman J. Scott Weese Joyce Rousseau Jason W. Stull 《Zoonoses and public health》2019,66(7):861-870
Swine are known reservoirs for Clostridioides difficile, formerly known as Clostridium difficile, and transmission from swine to human farm workers is strongly suggested by previous studies. This cross‐sectional study evaluated the potential role of farm environmental surfaces, including those in worker breakrooms and swine housing areas, in the possible transmission of C. difficile from swine to farm workers. Environmental surfaces and piglet faeces at 13 Ohio swine farms were sampled in 2015. Typical culturing techniques were performed to isolate C. difficile from samples, and amplification of toxin genes (tcdA, tcdB and cdtB) and PCR‐ribotyping were used to genetically characterize recovered isolates. In addition, sequencing of toxin regulatory gene, tcdC, was done to identify the length of identified deletions in some isolates. A survey collected farm‐level management risk factor information. Clostridioides difficile was recovered from all farms, with 42% (188/445) of samples testing positive for C. difficile. Samples collected from all on‐farm locations recovered C. difficile, including farrowing rooms (60%, 107/178), breakrooms (50%, 69/138) and nursery rooms (9%, 12/129). Three ribotypes recovered from both swine and human environments (078, 412 and 005) have been previously implicated in human disease. Samples taken from farrowing rooms and breakrooms were found to have greater odds of C. difficile recovery than those taken from nursery rooms (OR = 40.5, OR = 35.6, p < .001 respectively). Farms that weaned ≥23,500 pigs per year had lower odds of C. difficile recovery as compared to farms that weaned fewer pigs (OR = 0.4, p = .01) and weekly or more frequent cleaning of breakroom counters was associated with higher odds of C. difficile recovery (OR = 11.7, p < .001). This study provides important insights into the presence and characterization of C. difficile found in human environments on swine farms and highlights how these areas may be involved in transmission of C. difficile to swine farm workers and throughout the facility. 相似文献
993.
van den Oord QG van Wijk EJ Lugton IW Morris RS Holmes CW 《New Zealand veterinary journal》1995,43(7):328-332
Two groups of six mature brushtail possums (Trichosurus vulpeculu) were housed in two respiration chambers, and their heat production, whole body conductance and lower critical temperatures were measured under a variety of simulated weather patterns. The possums were subjected to ambient temperatures of 30, 20 and 3 degrees C. At 20 and 3 degrees C, the animals were exposed to near still air and light winds (wind speed 0.8 and 6.7 km/h), both with, and without, simulated rain every 8 hours. The lower critical temperature in near still air lies between 7 and 10 degrees C. This temperature increases by about 2, 6 and 8 degrees C respectively for a wind velocity of 6.7 km/h, simulated rain and a combination of the two factors. Weather in New Zealand, especially in the cooler part of the year, will often produce conditions below the lower critical temperature of the thermoneutral zone of possums. This will necessitate significant increases in metabolic rate and hence food consumption or mobilisation of body fat reserves, which if not sustainable will result in the death of possums. Field studies have shown that this is often the case in the wild. It is proposed that this stress may be sufficient to decrease the resistance (especially cell-mediated immunity) of some possums and allow acceleration of the disease process in those infected with Mycobacterium bovis. 相似文献
994.
995.
Roslyn G. Machon B.V.Sc. M.V.Sc. M.S. Marc R. Raffe D.V.M. M.S. Elaine P. Robinson B.Vet.Med. M.V.Sc. 《Journal of Veterinary Emergency and Critical Care》1995,5(2):121-129
The caudla vena cava (CVA) was evaluated as an laternative site for the measurement of central venous pressure (CPV) in six healthy, sedated (ketamine 10 mg/km, midazolam 0.1 mg/kg, and atropine 0.04 mg/kg IM) cats. The CVC was cathererized via medial saphenous puncture, and estimates of CPV from this site compared to those obtained via a jugular catheter. Simulataneous CPV values were recorded electronically (mmgh), via calibrated pressure transducer positioned at the level of the manubrium in cats in lateral recumbency. Five readings, performed at 1 minute intervals, were collected from the jugular and CVC catheters at rest (baseline) and following a rapid fluid bolus. Twenty-four hours later, cats were resedated, baseline measurements repeated, and CVPs recorded following a rapid, 25% whole-blood volume bleed. CVP measurements from the jugular and CVC were statistically compared using repeated measures ANOVA (p<0.05). There were no significant differences between the two sites in the baseline and bleed trials. Significant defferences between jugular and CVC CVPs were noted at 1 and 2 minutes following the fluid bolus. It was concluded that CVC is an alternative site for measurement of CPV in sedated cats. 相似文献
996.
A crossbreeding experiment was carried out to determine the sire breed effects on fattening performance of fat-tailed lambs.
Rams from four Iranian fat-tailed sheep breeds, namely Kurdi (K), Afshari (A), Chaal (C) and Sanjabi (S) were mated to Kurdi
ewes producing 454 progeny of four genotype groups (KK, AK, CK and SK). A total of 80 lambs comprising ten female and ten
male lambs from each genotype group were used in a fattening experiment during a period of 88 days. Genotype of the lamb had
significant effect on average dry matter intake (ADMI) (P < 0.05). SK lambs had the lowest ADMI. Average daily gain (ADG) and food conversion ratio (FCR) were not influenced by genotype
of the lamb. Sex of the lamb had significant effect on both ADG and FCR (P < 0.01). Slaughter weight (SLW), hot carcass weight (HCW), cold carcass weight (CCW) and daily carcass gain (DCG) were not
affected by lamb's genotype. However, genotype had significant effect on dressing percentage, carcass loss percentage (P < 0.01), carcass length (CL), leg circumference (LC), carcass compactness (CC) (P < 0.01) and carcass density (CD) (P < 0.05). Carcass width (CW) and leg length (LL) were not affected by lamb's genotype. Sex of the lamb had significant effects
on CL, CW, LC, CC, CD (P < 0.01) and LL (P < 0.05). The results indicated the existence of sire breed difference for daily dry matter intake and carcass conformation
in the fat-tailed sheep. 相似文献
997.
998.
Combined Campylobacter jejuni and Campylobacter coli Rapid Testing and Molecular Epidemiology in Conventional Broiler Flocks 下载免费PDF全文
G. Schallegger S. Muri‐Klinger K. Brugger C. Lindhardt L. John M. Glatzl M. Wagner B. Stessl 《Zoonoses and public health》2016,63(8):588-599
Campylobacter spp. are important causes of bacterial zoonosis, most often transmitted by contaminated poultry meat. From an epidemiological and risk assessment perspective, further knowledge should be obtained on Campylobacter prevalence and genotype distribution in primary production. Consequently, 15 Austrian broiler flocks were surveyed in summer for their thermophilic Campylobacter spp. contamination status. Chicken droppings, dust and drinking water samples were collected from each flock at three separate sampling periods. Isolates were confirmed by PCR and subtyped. We also compared three alternative methods (culture‐based enrichment in Bolton broth, culture‐independent real‐time PCR and a lateral‐flow test) for their applicability in chicken droppings. Twelve flocks were found to be positive for thermophilic Campylobacter spp. during the entire sampling period. Seven flocks (46.6%) were contaminated with both, C. jejuni and C. coli, five flocks harboured solely one species. We observed to a majority flock‐specific C. jejuni and C. coli genotypes, which dominated the respective flock. Flocks within a distance <2 km shared the same C. jejuni genotypes indicating a cross‐contamination event via the environment or personnel vectors. Multilocus sequence typing (MLST) of C. jejuni revealed that the majority of isolates were assigned to globally distributed clonal complexes or had a strong link to the human interface (CC ST‐446 and ST4373). The combination of techniques poses an advantage over risk assessment studies based on cultures alone, as, in the case of Campylobacter, occurrence of a high variety of genotypes might be present among a broiler flock. We suggest applying the lateral‐flow test under field conditions to identify ‘high‐shedding’ broiler flocks at the farm level. Consequently, poultry farmers and veterinarians could improve hygiene measurements and direct sanitation activities, especially during the thinning period. Ultimately, real‐time PCR could be applied to quantify Campylobacter spp. directly from chicken droppings and avoid non‐interpretable results achieved by culture‐dependent methods. 相似文献
999.
Kagawa S Klein F Corboz L Moore JE Murayama O Matsuda M 《Veterinary research communications》2001,25(7):565-575
Forty-six isolates of Taylorella equigenitalis were analysed by pulsed-field gel electrophoresis (PFGE) after separate digestion of the genomic DNA with ApaI and with NotI. The isolates had been obtained from horses in six European countries and were classified into 18 genotypes. In Belgium, 2 genotypes were detected in 2 isolates, in England 9 among 15, in Finland 2 in 2, in France 2 among 10, in Sweden 3 among 5, and in Switzerland 3 among 12. Two English isolates and 4 French isolates gave identical PFGE profiles to those of Kentucky 188 from the United States. A common genotype was found in 5 isolates from Belgium and England and also in 10 isolates from France and Switzerland. The analysis of genomic DNA from 12 isolates of T. equigenitalis obtained from male horses in France, Sweden and Switzerland gave no evidence of a sex-related difference in the genomic DNA. Genomic DNA from 11 streptomycin (STM)-susceptible isolates obtained in Sweden and Switzerland were classified into four genotypes by PFGE. Each of the six genotypes determined among the 17 isolates from these two countries had single phenotypes for resistance or susceptibility to STM. 相似文献
1000.
Experimental Mycotoxic Nephropathy in Pigs Provoked by a Diet Containing Ochratoxin A and Penicillic Acid 总被引:8,自引:0,他引:8
Stoev SD Vitanov S Anguelov G Petkova-Bocharova T Creppy EE 《Veterinary research communications》2001,25(3):205-223
Mycotoxic nephropathy was induced in 18 young pigs by diets contaminated with strains of Aspergillus ochraceus containing ochratoxin A (OTA) and penicillic acid (PA) at levels corresponding to those naturally encountered in animal feeds in Bulgaria. Haematological and biochemical parameters, as well as the morphological and ultrastructural changes in various internal organs, and especially in the kidneys, were examined at different stages of development of the disease. A mottled surface of the kidneys was only seen in pigs exposed to a mouldy diet containing 180 ppb OTA for 3 months, but microscopic lesions, as well as changes in various haematological and biochemical parameters, were observed in all groups exposed to the same mouldy diet containing only 90 or 180 ppb OTA. Histological examination showed two types of change: degenerative changes affecting the epithelial cells of the proximal tubules, which predominated at the initial stage, and proliferative changes in the interstitium, which predominated at the later stage of the disease. Telangiectasis and lymph stasis were also seen, as well as degenerative changes in the capillary endothelium. The characteristic renal lesions were similar to those observed in spontaneous cases of mycotoxic porcine nephropathy in Bulgaria, but they were a little different from the classic Danish porcine nephropathy. The enhanced toxicity of OTA in our study may be due to a synergistic effect between OTA and PA or to some other unknown metabolites produced by the same ochratoxinogenic strains of A. ochraceus. 相似文献