Mucus synthesis in the goblet cells of the small intestine in experimental infection with the coccidium Isospora suis in piglets

The state of mucus synthesis in the goblet cells of the small intestine was studied in conventional piglets infected with a dose of 200,000 oocytes of the coccidium Isospora suis the first and fifth day after parturition. The synthesis of mucus and its chemical characteristics undergo significant changes during the third and fourth day after infection. The activity of acid and neutral mucous substances declines; their level and the physiological synthetic function of goblet cells begin to return to the normal during the period starting on the eight to tenth day after infection. However, there were no fully functioning goblet cells in the broken numerical ratio even at the end of the period of investigation, i.e. the 13th day after infection. The thin surface layer of mucus remained almost unchanged within the whole extent of the small intestine parts studied.     

P‐33 Poxvirus infection in two cats

A 3‐year‐old Burmese cat was presented with a history of nonresolving crusted and papular lesions of the face and prior treatment with prednisolone. Skin biopsies revealed typical pox lesions with hyperplasia and ulceration of the epidermis and eosinophilic cytoplasmic inclusion bodies in the epidermal cells. In the upper dermis there was prominent diffuse mast cell infiltration and mild neutrophilic and eosinophilic inflammation. Rare cytoplasmic inclusion bodies were also present in swollen endothelial cells of dermal venules, which showed no other degenerative changes. Histological diagnosis was confirmed by electron microscopic evidence of pox virus particles in inclusion bodies of epidermal cells. The lesions resolved within 6 weeks with systemic antibiotic therapy and supportive care. A 2‐year‐old domestic short‐haired cat was presented with multiple disseminated papular and ulcerative pox lesions with central eschar over the entire body. Histologically, large epidermal inclusion bodies (up to 6 μm in diameter) were present. Widespread haemorrhage and vascular wall necrosis was visible in the dermis and subcutis. Some subcutaneous vessels showed neutrophilic vasculitis. In addition to diffuse dermal neutrophilic and eosinophilic inflammation, a lymphohistiocytic panniculitis was also present. The cat died as a result of massive haemorrhage and lymphedema, despite supportive care. Funding: Self‐funded.     

Response of the lactating and postweaning sow to gonadotropin releasing hormone (GnRH)

Clinical and endocrinological responses to administration of gonadotropin releasing hormone analog (LH-RH-A) during the lactation period and postweaning in the sow were investigated. Plasma LH concentrations in lactating sows rose immediately after administration of LH-RH-A. However, in postweaning sows the increase of LH level was more slowly. Three of 5 postweaning sows came into estrus and ovulated after LH-RH-A treatment. One sow exhibited a distinct LH response, but her ovaries remained quiescent. The remaining one with feeble estrus for a short period became cystic ovaries. Thus, LH response to GnRH in the sow seems to be higher during early lactation than at 2 days postweaning.     

Confirmation of rinderpest in experimentally and naturally infected cattle using microtitre techniques

Summary Virulent rinderpest virus was detected by immunoperoxidase staining of microtitre bovine kidney cell cultures within 24 to 48 hours of inoculation with prescapular lymph node and spleen homogenates from experimentally infected steers. Rinderpest virus specific cytopathic effects were evident from 48 hours in microtitre plates and from 72 hours in rolled tube cultures. Nasal and ocular secretions collected from cattle naturally infected with rinderpest and inoculated into bovine kidney cell cultures did not readily yield cytopathic virus in both tubes and microtitre plates, but immunoperoxidase staining of microtitre cultures on the fourth day of inoculation detected replication of virus in cultures inoculated with ocular and nasal secretions from seven of 17 cattle tested.

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Resumen Se detectó el virus virulente de mediante la tinción con inmunoperoxidasa de cultivos de células de ri?ón bovino en bandejas de microtitulación, después de la inoculación de estos con suspensiones homogenizadas de ganglios linfáticos preescapulares y de bazo provenientes de novillos infectados experimentalmente. El efecto citopático del virus de la peste bovina fue evidente desde las 48 horas en bandejas de microtitulación y desde las 72 horas en tubos de cultivo giratorios. Secreciones oculares y nasales colectadas de ganado infectado en forma natural con la peste bovina e inoculadas en cultivos de células de ri?ón bovino, no mostraron efecto citopático fácilmente en tubos giratorios o bandejas de microtitulación, pero la tinción de las bandejas con inmunoperoxidasa reveló replicación del virus a partir del cuarto día de inoculación con secreciones oculares y nasales en siete de los 17 animales examinados.

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Résumé Un virus bovipestique virulent a été décelé par le test de coloration à l'immunoperoxydase de cellules rénales bovines en culture dans des plaques de microtitrage et infectées 48 heures plus t?t avec des homogénats de ganglions lymphatiques et de rate provenant de bouvillons infectés expérimentalement. Les effets cytopathogènes du virus étaient évidents au bout de 48 h dans les plaques de microtitrage et 72 h dans les tubes en rollers. Les sécrétions nasales et oculaires prélevées sur du bétail infecté naturellement par la peste bovine et inoculées sur des cellules rénales bovines n'ont pas toujours montré d'effet cythopathogène aussi bien dans les tubes que dans les plaques de microtitrage. Cependant, la coloration à la peroxydase au jour 4 après l'inoculation a permis de déceler la présence de virus dans 7 cas sur 17.

     

Additional diagnostic procedures

This article reviews recent diagnostic procedures that have arisen over the last 10 years. Videoendoscopy of horses on a high-speed treadmill allows observation of some of the changes that take place in a horse's airway during exercise. Measurements of upper airway airflows and transupper airway pressure, the use of an esophageal balloon and a Ventigraph to measure changes in pleural pressure, and pulmonary function testing are new techniques that aid the researcher in understanding the mechanics and pathologic characteristics of airway diseases and help the practitioner in assessing the severity of a problem, measuring response to therapy, and accurately determining a prognosis.     

Participation of H1-receptors in histamine-induced contraction and relaxation of horse coronary artery in vitro.

The mechanisms of histamine-induced contraction and relaxation were investigated in rings isolated from a middle part of the left descending coronary arteries of horses. Intact and endothelium-denuded preparations were compared. Rings of horse coronary arteries contracted in response to histamine in a concentration dependent manner, but some of them relaxed with lower concentrations and contracted with higher concentrations. Removal of the endothelium abolished the relaxation and potentiated the contraction. The pD2 values were 4.70 +/- 0.08 in the rings with intact endothelium and 4.95 +/- 0.08 in endothelium-denuded rings. Histamine-induced contractions in intact and denuded preparations were not affected by an H2-antagonist, cimetidine, but were inhibited by an H1-antagonist, diphenhydramine in non-competitive manner in the rings with endothelium and in competitive manner in denuded rings. After precontraction with PGF2 alpha or norepinephrine, histamine relaxed preparations with intact endothelium (pD2 value, 7.80 +/- 0.11), although histamine-induced relaxations were not observed in denuded preparations. The relaxation was competitively inhibited by diphenhydramine. Relaxing response was significantly attenuated by methylene blue, quinacrine, L-nitro-arginine, gossypol and AA861 but not by indomethacin. These results suggest that the histamine-induced contraction and relaxation in horse coronary arteries are mediated mainly by H1-receptors in the smooth muscle and endothelium, respectively, and H1-receptor activation of endothelial cells may liberate vasodilator substances.     

Standing surgical procedures of the foot.

Several surgical conditions affecting the foot can be successfully managed with the horse standing. Many factors affect the clinician's choice of whether to perform surgery with the horse standing or anesthetized. Temperament or pregnancy of the horse and economics may influence the decision to perform surgery with the horse standing. This article discusses several foot conditions that can be treated successfully in the ambulatory horse.