全文获取类型
收费全文 | 137423篇 |
免费 | 7841篇 |
国内免费 | 5265篇 |
专业分类
林业 | 9603篇 |
农学 | 11447篇 |
基础科学 | 5206篇 |
19262篇 | |
综合类 | 27166篇 |
农作物 | 5785篇 |
水产渔业 | 6667篇 |
畜牧兽医 | 53882篇 |
园艺 | 2649篇 |
植物保护 | 8862篇 |
出版年
2022年 | 1540篇 |
2021年 | 2537篇 |
2020年 | 2405篇 |
2019年 | 2690篇 |
2018年 | 2717篇 |
2017年 | 3426篇 |
2016年 | 3257篇 |
2015年 | 3629篇 |
2014年 | 3757篇 |
2013年 | 6225篇 |
2012年 | 5899篇 |
2011年 | 6827篇 |
2010年 | 5390篇 |
2009年 | 5189篇 |
2008年 | 6105篇 |
2007年 | 5852篇 |
2006年 | 5726篇 |
2005年 | 5250篇 |
2004年 | 3706篇 |
2003年 | 3535篇 |
2002年 | 3147篇 |
2001年 | 4185篇 |
2000年 | 4289篇 |
1999年 | 3875篇 |
1998年 | 2167篇 |
1997年 | 2054篇 |
1996年 | 1820篇 |
1995年 | 1882篇 |
1994年 | 1754篇 |
1993年 | 1665篇 |
1992年 | 2549篇 |
1991年 | 2495篇 |
1990年 | 2334篇 |
1989年 | 2245篇 |
1988年 | 1864篇 |
1987年 | 1778篇 |
1986年 | 1716篇 |
1985年 | 1599篇 |
1984年 | 1271篇 |
1983年 | 1066篇 |
1982年 | 747篇 |
1979年 | 1068篇 |
1978年 | 826篇 |
1975年 | 816篇 |
1974年 | 898篇 |
1973年 | 864篇 |
1972年 | 806篇 |
1971年 | 796篇 |
1970年 | 800篇 |
1969年 | 780篇 |
排序方式: 共有10000条查询结果,搜索用时 609 毫秒
961.
不同施肥期沾化冬枣对15N的吸收、分配及利用特性 总被引:14,自引:1,他引:14
盆栽条件下利用15N示踪技术, 研究不同时期施15N - 尿素, 对沾化冬枣15N的吸收利用及分配特性的影响。结果表明: 生长季前期(萌芽前和花前) 施用15N - 尿素, 经根系吸收后, 15N优先分配到贮藏器官(包括主干、多年生枝和粗根) 中, 然后外运用于树体新生器官(包括枣吊及其叶片、新生营养枝、细根及果实) 的形成, 果实采收后15N开始向贮藏器官回流; 果实硬核期15N直接用于树体营养生长和生殖生长, 而不是先贮藏再利用; 果实速长期15N优先向贮藏器官中积累; 萌芽前施15N在树体内的运转规律符合落叶果树贮藏N营养分配规律, 优先转运到生长中心。随着施肥期的后延, 植株对15N - 尿素的当季利用率逐渐下降。 相似文献
962.
红千层的离体培养及快速繁殖 总被引:3,自引:0,他引:3
研究了红千层离体快繁的若干影响因素。结果表明: 叶片在MS + 6-BA 115 mg·L - 1+NAA015 mg·L - 1中的愈伤组织诱导率43% , 并可直接从愈伤组织表面分化出不定芽, 分化率为69.2%; 腋芽启动培养基为MS + 6-BA 1~1.5 mg·L - 1 + NAA 0.5 mg·L - 1 , 萌动率为77%; 继代和增殖培养基为MS + 6-BA 1 mg·L - 1 +NAA 0.25 mg·L - 1 , 平均增殖系数为16.7; 生根壮苗培养基为1 /2MS +NAA 0.25 mg·L - 1 , 生根率96.1%; 试管苗生根培养30~35 d移栽, 成活率最高可达90%以上。 相似文献
963.
964.
A. B. De Nardi C. R. Daleck C. H. M. Souza R. L. Amorin S. Rodaski C. Calderon R. Torres 《Veterinary and comparative oncology》2005,3(1):56-57
Introduction: Cyclooxygenase‐2 (Cox‐2) is the inducible form and the rate‐limiting enzyme, for conversion of arachidonic acid to prostaglandins. Cox‐2 overexpression, common in carcinomas, is associated with increased growth rate, resistance to apoptosis, angiogenesis, and overall, both local and distant aggressive behavior. Cox‐2 overexpression has been detected in human and canine mammary tumors (MTs). Histopathology of canine MT is not always predictive of biologic behavior, and anecdotally, only 50% of the malignant MTs are expected to metastasize. We hypothesize that Cox‐2 expression correlates with aggressive behavior.
Methods: This retrospective study evaluated 48 bitches, presented for excision of MT between 2000 and 2003 at FMVZ de Botucatu‐UNESP, Brasil. Follow‐up varied from 18 months to 24 months and included physical examination and thoracic radiographs. Histopathologic examination was performed in all tumors, as well as in metastatic lesions when detected in the follow‐up period. Immunohistochemistry was used to detect expression of Cox‐2 in paraffin blocks (Rabbit polyclonal anti‐PGHS‐2. Oxford Biomedical). 10 adenomas, 10 carcinomas, 10 benign mixed tumors, 10 malignant mixed tumors and 8 cases of primary carcinomas and their metastatic lesions.
Results: Expression of Cox‐2 varied among groups. Adenomas (32.1%), mixed benign tumors (38%), carcinomas (60.3%), malignant mixed tumors (65.8%), and metastatic carcinomas (81.25%) and their metastatic lesions (84.35%). Statistically significant differences (p < 0.05) were observed between the benign and malignant counterparts and between carcinomas and metastatic carcinomas.
Conclusions: Cox‐2 expression correlates with both histologic and biologic behavior in mammary carcinomas, and may serve as a predictor of metastatic potential. 相似文献
Methods: This retrospective study evaluated 48 bitches, presented for excision of MT between 2000 and 2003 at FMVZ de Botucatu‐UNESP, Brasil. Follow‐up varied from 18 months to 24 months and included physical examination and thoracic radiographs. Histopathologic examination was performed in all tumors, as well as in metastatic lesions when detected in the follow‐up period. Immunohistochemistry was used to detect expression of Cox‐2 in paraffin blocks (Rabbit polyclonal anti‐PGHS‐2. Oxford Biomedical). 10 adenomas, 10 carcinomas, 10 benign mixed tumors, 10 malignant mixed tumors and 8 cases of primary carcinomas and their metastatic lesions.
Results: Expression of Cox‐2 varied among groups. Adenomas (32.1%), mixed benign tumors (38%), carcinomas (60.3%), malignant mixed tumors (65.8%), and metastatic carcinomas (81.25%) and their metastatic lesions (84.35%). Statistically significant differences (p < 0.05) were observed between the benign and malignant counterparts and between carcinomas and metastatic carcinomas.
Conclusions: Cox‐2 expression correlates with both histologic and biologic behavior in mammary carcinomas, and may serve as a predictor of metastatic potential. 相似文献
965.
S. Murphy A. M. Hayes L. Blackwood G. Maglennon H. Pattinson A. H. Sparkes 《Veterinary and comparative oncology》2005,3(4):222-229
A retrospective study was undertaken of dogs presented to the Animal Health Trust for treatment of oral malignant melanoma, without radiographic evidence of pulmonary metastases. Group 1 (n = 13) received radiotherapy of the primary and any lymph node metastases (4 weekly fractions of 9 Gy); and group 2 (n = 15) were treated the same but additionally received between two and six doses carboplatin at 300 mg m?2 every 3 weeks. Median survival times for the two groups were 307 and 286 days, respectively (P > 0.05). In addition, carboplatin therapy did not significantly reduce the proportion of dogs dying due to metastases (three from group 1 and four from group 2). We found no evidence of a beneficial effect of carboplatin therapy over radiotherapy alone. 相似文献
966.
We describe the spatial epidemiology of Varroa destructor infestation among honey bee apiaries in the greater Auckland area of the North Island of New Zealand. The study population was comprised of 641 apiaries located within the boundaries of the study area on 11 April 2000. Cases were those members of the study population declared Varroa-infested on the basis of testing conducted between April and June 2000. The odds of Varroa was highest in apiaries in the area surrounding transport and storage facilities in the vicinity of Auckland International Airport. A mixed-effects geostatistical model, accounting for spatial extra-binomial variation in Varroa prevalence, showed a 17% reduction in the odds of an apiary being Varroa infested for each kilometre increase in the squared distance from the likely site of incursion (95% Bayesian credible interval 7–28%). The pattern of spatially autocorrelated risk that remained after controlling for the effect of distance from the likely incursion site identified areas thought to be ‘secondary’ foci of Varroa infestation initiated by beekeeper-assisted movement of infested bees. Targeted investigations within these identified areas indicated that the maximum rate of local spread of Varroa was in the order of 12 km/year (interquartile range 10–15 km/year). 相似文献
967.
Forty Swiss Large White piglets of 4 weeks of age were used to determine the effect of dietary Chinese rhubarb on growth performance, as well as on energy and nitrogen metabolism by means of indirect respiration calorimetry studies. A cereal-based diet, supplemented with four different dosages of Chinese rhubarb (0.0%, 0.25%, 0.5%, 1%), was offered ad libitum to the piglets. Gaseous exchange was measured, and urine, faeces, and blood samples were taken. The addition of 0.25% dietary rhubarb increased feed intake (+32%) and daily weight gain (+67%) compared to the diet containing the greatest amount of rhubarb (1%). The piglets fed diet supplemented with 0.25% rhubarb digested and metabolised more energy and nitrogen relative to the animals of treatment 1%. As a consequence, the same group (0.25%) converted more energy and nitrogen into body protein than piglets receiving diet supplemented with 1% rhubarb. However, compared to the control group, the addition of 0.25% rhubarb to the diet resulted in not significantly alter growth performance, energy, and nitrogen metabolism of the animals.Blood parameters were not affected by the rhubarb supplementation. The dose-responding effect of Chinese rhubarb was reflected by the dry matter content of faecal samples. According to the laxative effect of higher doses of rhubarb, the addition of 1% rhubarb to the diet reduced dry matter content of faeces. This effect was well visible in practice, although not relevant in statistical terms. The addition of 0.5% dietary rhubarb had no relevant effect on growth performance or on energy and nitrogen metabolism of treated animals. 相似文献
968.
Co-mingling weaned pigs often results in aggressive behavior which can result in injury and reduced performance. This experiment examined the effect of Acclimate™, a product reported to reduce equine aggression, on weaned pig aggression and growth performance. Weanling pigs (n=214, avg. wt.=5.93 kg) were blocked by weight, sex and litter (n=25) and randomly assigned to either Acclimate™ treatment or a control group. Acclimate™ treated pigs were marked on the nose, face and neck. Pigs were individually identified and videotaped to determine frequency of aggressive sequence (FAS) and duration of aggression (DOA) on an individual pig basis. Pig weights and feed consumption were used to determine feed intake, total weight gain (TWG), average daily gain (ADG) and feed to gain ratio (F/G). Differences between litter, sex and treatment for FAS, DOA, F/G and ADG were tested using GLM, SAS. Neither Acclimate™ treatment or sex had a significant effect on FAS, TWG or F/G. However, there was a trend towards reduced duration and intensity of aggression in Acclimate™ treated pigs (p=0.1). There was a significant effect of litter (p<0.01) and TWG (p<0.001) on FAS at both 1 and 3 weeks. Acclimate™ treatment of weaned pigs does not decrease FAS, but may decrease the intensity and duration of aggression without negatively effecting growth. 相似文献
969.
970.