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Chamali DAS Bimol Chandra ROY Ichiro OSHIMA Hideyuki MIYACHI Shotaro NISHIMURA Hisao IWAMOTO Shoji TABATA 《Animal Science Journal》2010,81(2):252-263
Varying chicken growth rates were induced with different nutritional regimes, and the collagen content and architecture of M. pectoralis (PT) were compared among 21‐day‐old chicks and broilers at 80 or 95 days of age. The percentage of muscle weight to live weight was higher in rapid growing chicks (8.4%) than slow growing chicks (6.3%). The 80‐day‐old broilers engaged in compensatory growth after the early slow growth period producing PT muscle at 11% of live weight. The 80‐ and 95‐day‐old chicks with restricted late growth after an early rapid growth period showed PT weight at 8% and 9% of live weight, respectively. Collagen content of the PT muscle markedly decreased from the chicks to the broilers. The collagen concentration was higher in the late‐growth restricted broilers (1.67–1.88 mg/g) than the compensatory growth broilers (1.01–1.10 mg/g). Collagen concentration did not differ between the rapid and slow growing chicks (2.72 and 2.94 mg/g). Scanning electron micrographs showed thick and thin perimysia, and honeycomb endomysia. In the perimysia, a stack layer of collagen platelets and a reticular layer of collagen fiber cords were distinguished and collagen baskets of adipocytes were observed. The perimysial collagen fibers became thicker during growth of the chicks to broilers. However, in the late‐growth restricted broilers, the perimysial collagen fibers seemed to have retarded development compared with the compensatory growth birds. The PT muscle of chickens develops optimally when body growth is enhanced. The PT muscle of the compensatory growth broilers had improved collagen architecture regardless of the marked decrease in collagen content. 相似文献
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FADHILLAH Shin YOSHIOKA Ryo NISHIMURA Kiyoshi OKUDA 《The Journal of reproduction and development》2014,60(3):194-201
To determine whether hypoxia has an effect on luteinization, we examined the influence of hypoxia on a model of bovine
luteinizing and non-luteinizing granulosa cell culture. The granulosa cells were obtained from small antral follicles (≤ 6 mm
in diameter). To induce luteinization, the cells were treated for 24 h with insulin (2 µg/ml), forskolin (10 µM) or insulin
in combination with forskolin at 20% O2. After 24 h, progesterone (P4) production was higher in the treated cells,
which we defined as luteinizing granulosa cells, than in non-treated cells, which we defined as non-luteinizing granulosa
cells. P4 production by non-luteinizing granulosa cells was not affected by hypoxia (24 h at 10% and 5% O2), while
P4 production by granulosa cells treated with insulin in combination with forskolin was significantly increased under hypoxia
(24 h at 10% and 5% O2). Because hypoxia affected P4 production by the luteinizing granulosa cells but not by the
non-luteinizing granulosa cells, hypoxia seems to promote P4 production during, rather than before, luteinization. In the
cells treated with insulin in combination with forskolin, mRNA and protein expression of steroidogenic acute regulatory
protein (StAR) and protein expression of 3β-hydroxysteroid dehydrogenase (3β-HSD) increased under 10% O2, while
mRNA and protein expressions of key protein and enzymes in P4 biosynthesis did not increase under 5% O2. The
overall results suggest that hypoxia plays a role in progressing and completing the luteinization by enhancing P4 production
through StAR as well as 3β-HSD expressions in the early time of establishing the corpus luteum. 相似文献
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Takanori NISHIMURA Ayaka TANEICHI Jun-ichi WAKAMATSU Akihito HATTORI 《Animal Science Journal》2003,74(5):399-405
The effect of skeletal muscle decorin on collagen fibrillogenesis was investigated, in order to provide background for understanding the functions of decorin in skeletal muscle. The self‐assembly of type I and III collagen with the addition of decorin or the core protein of decorin from bovine neonatal skeletal muscle was monitored using a spectrophotmeter. Time course changes in the absorbance of collagen solutions showed typical sigmoidal curves composed of three phases. The time of the initial phase was not different between the collagen solution with decorin and that without decorin. The increase rate of the absorbance in the second phase decreased with concentration of decorin added in collagen solutions. Similar effects on fibrillogenesis of type I and III collagens were observed when the core protein of decorin was added in collagen solutions. These results suggest that regulation of collagen fibrillogenesis by decorin depends on its core protein. The networks of reconstructed collagen fibrils with decorin were looser than those without decorin. Bovine skeletal muscle decorin could participate in the regulation of collagen fibrillogenesis and in the arrangement of collagen fibrils in the intramuscular connective tissue. 相似文献
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