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111.
The aim of this study was to first evaluate whether the chitosan hydrochloride-genipin crosslinking reaction is influenced by factors such as time, and polymer/genipin concentration, and second, to develop crosslinked drug loaded microspheres to improve the control over drug release. Once the crosslinking process was characterized as a function of the factors mentioned above, drug loaded hydrochloride chitosan microspheres with different degrees of crosslinking were obtained. Microspheres were characterized in terms of size, morphology, drug content, surface charge and capacity to control in vitro drug release. Clarithromycin, tramadol hydrochloride, and low molecular weight heparin (LMWH) were used as model drugs. The obtained particles were spherical, positively charged, with a diameter of 1–10 μm. X-Ray diffraction showed that there was an interaction of genipin and each drug with chitosan in the microspheres. In relation to the release profiles, a higher degree of crosslinking led to more control of drug release in the case of clarithromycin and tramadol. For these drugs, optimal release profiles were obtained for microspheres crosslinked with 1 mM genipin at 50 ºC for 5 h and with 5 mM genipin at 50 ºC for 5 h, respectively. In LMWH microspheres, the best release profile corresponded to 0.5 mM genipin, 50 ºC, 5 h. In conclusion, genipin showed to be eligible as a chemical-crosslinking agent delaying the outflow of drugs from the microspheres. However, more studies in vitro and in vivo must be carried out to determine adequate crosslinking conditions for different drugs. 相似文献
112.
Lucian Wielopolski Ruth D. Yanai Carrie R. Levine Sudeep Mitra Matthew A. Vadeboncoeur 《Forest Ecology and Management》2010
Forest soils are pivotal to understanding global carbon (C) cycling and evaluating policies for mitigating global change. However, they are very difficult to monitor because of the heterogeneity of soil characteristics, the difficulty of representative sampling, and the slow time scale of response to environmental change. Here we demonstrate that use of gamma-ray spectroscopy facilitates in situ non-destructive analysis of C and other elements in forest soils. In this approach the element-specific gamma-rays are induced by fast and thermal neutrons interacting with the nuclei of the elements present in the soil. Background gamma-rays emanating from naturally occurring radionuclides in the forest are recorded as well. We applied this approach in a mature northern hardwood forest on glacial till soils at the Bartlett Experimental Forest in New Hampshire, USA. The inelastic neutron scattering (INS) system yielded strong signals in gamma-ray counts/h, from C and other elements present in the soil matrix that included silicon, oxygen, hydrogen, iron, aluminum, manganese and potassium. The INS sensitivity for carbon was 20.656 counts h−1 kg−1 C m−2 based on current net C gamma-ray counts and the data for the O horizon and mineral soil to a depth of 30 cm obtained from a nearby quantitative soil pit (7.35 kg C m−2). We estimate the minimum detectable change to be ∼0.34 kg C m−2, which is ∼5% of the current soil C content, and the minimum detectable limit to be ∼0.23 kg C m−2. Eight % reproducibility from 11 measurements was limited, in part, by the large variability in the system counting geometry due to the uneven forest microtopography. The INS approach has the potential to revolutionize belowground monitoring of C and other elements, because the possibility of detecting a 5% change in forest soils has not been possible with destructive sampling methods. 相似文献
113.
114.
Nineteenth century narratives reveal historic catch rates for Australian snapper (Pagrus auratus)
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Snapper (Pagrus auratus) is widely distributed throughout subtropical and temperate southern oceans and forms a significant recreational and commercial fishery in Queensland, Australia. Using data from government reports, media sources, popular publications and a government fisheries survey carried out in 1910, we compiled information on individual snapper fishing trips that took place prior to the commencement of fisherywide organized data collection, from 1871 to 1939. In addition to extracting all available quantitative data, we translated qualitative information into bounded estimates and used multiple imputation to handle missing values, forming 287 records for which catch rate (snapper fisher?1 h?1) could be derived. Uncertainty was handled through a parametric maximum likelihood framework (a transformed trivariate Gaussian), which facilitated statistical comparisons between data sources. No statistically significant differences in catch rates were found among media sources and the government fisheries survey. Catch rates remained stable throughout the time series, averaging 3.75 snapper fisher?1 h?1 (95% confidence interval, 3.42–4.09) as the fishery expanded into new grounds. In comparison, a contemporary (1993–2002) south‐east Queensland charter fishery produced an average catch rate of 0.4 snapper fisher?1 h?1 (95% confidence interval, 0.31–0.58). These data illustrate the productivity of a fishery during its earliest years of development and represent the earliest catch rate data globally for this species. By adopting a formalized approach to address issues common to many historical records – missing data, a lack of quantitative information and reporting bias – our analysis demonstrates the potential for historical narratives to contribute to contemporary fisheries management. 相似文献
115.
116.
A.H. Halevy Ruth Shilo S. Simchon 《The Journal of Horticultural Science and Biotechnology》2013,88(4):427-434
2-Chloroethanephosphonic acid (Ethrel), an ethylene releasing compound, was used on gladiolus corms as a 30-minute dip treatment in various concentrations and at various times during storage.The most important effect of Ethrel was the marked increase in health and survival of treated plants infected with latent Fusarium.Ethrel at 1000 ppm enhanced sprouting when corms were stored at high temperatures or stored for a short period at 5 °C. Treatment of cold-stored corms at high concentrations delayed sprouting.Ethrel increased corm splitting, delayed flowering and slightly shortened flower stems. The size of harvested corms was reduced, but the yield of cormels was greatly increased by Ethrel. 相似文献
117.
M. B. Jackson P. S. Blackwell J. R. Chrimes T. V. Sims 《The Journal of Horticultural Science and Biotechnology》2013,88(3):439-448
Because water can restrict rapid diffusive exchange of gases we have examined how far the nutrient solution is adequately aerated in NFT systems used for glasshouse tomato production. Measurements were at various places of: the concentrations of dissolved O2 and dinitrogen oxide (nitrous oxide), the maximum rate of O2 flow to an O2-consuming sink (O2 flux density), and the electrochemical (redox) potential. Whalehide pots containing peat-based or peat-sand based composts used to stand the plants in the NFT solution were found waterlogged and poorly aerated. Dissolved O2 in solution drawn from the pots rarely exceeded 1-2 mg l-1. This small amount of O2 was associated with accumulations of dinitrogen oxide, a gaseous product of anaerobic microbial respiration. Oxygen flux density was also small but redox potentials remained above 300 mV which was too high to favour the formation of Phytotoxins such as Mn(II), Fe(II) or H2S. Replacing the composts with more open structured inorganic media such as Perlite, Rockwool or Capogro almost completely eliminated the symptoms of poor aeration observed in the composts. On one occasion the O2 dissolved in the flowing nutrient solution was found to be depleted by c. 50%. Thus the whole system can sometimes be subject to considerable O2 deficits. 相似文献
118.
A Tres G van der Veer MD Perez-Marin SM van Ruth A Garrido-Varo 《Journal of agricultural and food chemistry》2012,60(33):8129-8133
Organic products tend to retail at a higher price than their conventional counterparts, which makes them susceptible to fraud. In this study we evaluate the application of near-infrared spectroscopy (NIRS) as a rapid, cost-effective method to verify the organic identity of feed for laying hens. For this purpose a total of 36 organic and 60 conventional feed samples from The Netherlands were measured by NIRS. A binary classification model (organic vs conventional feed) was developed using partial least squares discriminant analysis. Models were developed using five different data preprocessing techniques, which were externally validated by a stratified random resampling strategy using 1000 realizations. Spectral regions related to the protein and fat content were among the most important ones for the classification model. The models based on data preprocessed using direct orthogonal signal correction (DOSC), standard normal variate (SNV), and first and second derivatives provided the most successful results in terms of median sensitivity (0.91 in external validation) and median specificity (1.00 for external validation of SNV models and 0.94 for DOSC and first and second derivative models). A previously developed model, which was based on fatty acid fingerprinting of the same set of feed samples, provided a higher sensitivity (1.00). This shows that the NIRS-based approach provides a rapid and low-cost screening tool, whereas the fatty acid fingerprinting model can be used for further confirmation of the organic identity of feed samples for laying hens. These methods provide additional assurance to the administrative controls currently conducted in the organic feed sector. 相似文献
119.
Federico Rossi Ruth M. Potrafka Ferran Garcia Pichel Roberto De Philippis 《Soil biology & biochemistry》2012
Biological soil crusts (BSCs) are highly specialized topsoil microbial communities commonly found in arid and semiarid environments, permeated by a polymeric matrix of polysaccharides. BSCs can in principle influence edaphic properties such as texture, pore formation and water retention, which in turn determine water distribution and biological activity in dry lands. This paper investigates the influence of biotic and abiotic factors on BSC hydraulic conductivity, a parameter gauging the ease with which water can move through the pore spaces. Texture, phototroph abundance, microbial composition, and extracellular carbohydrate content were considered as potentially relevant parameters in a correlational study of BSC samples that spanned 1.5 orders of magnitude in hydraulic conductivity. A newly developed, non-destructive extraction method enabled us to directly quantify the specific role of extracellular polysaccharides on soil permeability on a variety of samples. Hydraulic conductivity showed a strongest correlation with texture (positive with sand content, negative with silt and clay). A weaker negative correlation with carbohydrate content, especially with polysaccharides having a molecular weight < 100 kDa, was also detected. In multiple regression analyses texture (silt content) was sufficient to explain most of the variation in hydraulic conductivity However, experimental removal of polymeric carbohydrates, resulted invariably in a substantial decrease in hydraulic conductivity for any given sample (between 1.7 and 3.3 fold). Our results suggest that while soil texture determines overall hydraulic conductivity in BSCs, the presence of exopolysaccharides can significantly enhance it, likely by conferring a spongy structure to a BSC thus increasing the number of waterways within it. 相似文献
120.
Matthew K. Egbekun John I. Akowe Ruth J. Ede 《Plant foods for human nutrition (Dordrecht, Netherlands)》1996,49(4):301-306
The fresh ripe fruit pulp and syrup, produced using the open pan evaporator, ofVitex doniana were analysed physico-chemically. Results show that the edible pulp of the ripe fruit is fairly rich in vitamin C (18.1 mg/100 ml) but acidic (pH 4.38) and poor in protein (0.82%) and oil (0.56%). The syrup yielded a DE value of 52, total dissolved solids content of 67° Brix and a refractive index of 1.4762 at 20°C. The syrup was moderately visous (2.1 Poiseuille), acid (pH 4.45) but relatively poor in vitamin C (2.8 mg/100 ml) and protein (0.3%). Sensory evaluation showed no significant difference (p0.05) in taste, flavor and overall acceptability betweenV. doniana syrup and honey. Both physico-chemical and sensory results indicate that the syrup was highly desirable and can substitute for other syrups as a nutritive sweetener. 相似文献