To examine the effects of plastic film removal on grain yield and soil organic matter (SOM), a spring maize (Zea may L.) field experiment was conducted for 5 yr at Changwu Agricultural and Ecological Experimental Station of Northwest China. Compared with traditional plastic film mulching during entire growing stages (FM), plastic film removal at the silking stage (RM) resulted in a 6.3% higher average maize yield. Under the RM treatment, soil organic carbon and total nitrogen significantly increased after the 5‐yr cultivation in the 0‐ to 20‐cm layer. Significant increases in extractable organic C (EOC), KMnO4‐oxidizable C (KMnO4‐C) and C management index (CMI) in the 0‐ to 20‐cm layer, and light fraction organic C and EOC in the 20‐ to 40‐cm layer were observed in response to plastic film removal after the 1‐yr treatment; the responses were more significant after 5 yr. Under the RM treatment, significant increases in microbial biomass C, light fraction organic N, extractable organic N, KMnO4‐C and CMI were also observed after five years in the 20‐ to 40‐cm layer. Moreover, KMnO4‐C and EOC were much more sensitive than other labile SOM fractions to the application of RM, even after only 1 yr of cultivation. Therefore, compared with mulching for the whole growing season, plastic film removal at the maize silking stage is an effective option for increasing yields and enhancing SOM concentration and soil sustainability in the regions with semiarid monsoon climates that have sufficient rainfall during maize reproductive stages. 相似文献
A field experiment on permanent ryegrass–white clover pasture at AgResearch's Ruakura dairy farm near Hamilton, New Zealand quantified nitrous oxide (N2O) emissions from different types of dairy effluent applied to soil at three seasons and evaluated the potential of dicyandiamide (DCD) (a nitrification inhibitor) to decrease gaseous N2O emissions. Fresh or stored manure and farm dairy effluent (FDE; from dairy shed washings), with or without DCD (10 kg/ha), were applied at approximately 100 kg N/ha to plots on a well‐drained soil on volcanic parent material. A field chamber technique was used to measure N2O emissions. Application of manure or FDE, both in fresh and stored forms, to pasture generally increased N2O emissions. Overall N2O emission factors (EF) varied between 0.01% and 1.87%, depending on application season and effluent type. EFs in spring and autumn were greater than those in summer (P <0.05). Among the effluents, N2O EFs were largest from fresh FDE (1.65%) during the spring measurement period, stored manure (1.87%) during the autumn and stored FDE (0.25%) during the summer. DCD was effective in decreasing N2O EFs from fresh FDE, fresh manure, stored FDE and stored manure by 40–80%, 69–76%, 24–84% and 60–70%, respectively. DCD reduced N2O emissions during the spring and autumn seasons more effectively than in the summer season. 相似文献
Myostatin (MSTN) is an interesting negative growth‐regulating gene that has been well characterized in vertebrates but scantly described in invertebrates. The current study focuses on the downregulation of the MrMSTN gene and subsequently records any histological changes for giant freshwater prawn, Macrobrachium rosenbergii (Mr). In addition, the study also deals with the MrMSTN gene's influence on other growth‐related genes, which include myosin heavy chain, dystrophin‐dystroglycoprotein complex, tropomyosin, farnesoic acid o‐methyl transferase, arginine kinase, cyclophilin, and acyl CoA desaturase. The preliminary histological analysis following MrMSTN silencing favors muscle regeneration, which supports its functional role as a negative growth regulator and its significant effect on the expression of other growth‐related genes. Overall, our results show that the MrMSTN gene could therefore be a potential target for gene manipulation aimed at enhancing the growth and muscle development of M. rosenbergii, which could be beneficial in increasing the total mass production in the postlarva phase at the hatchery level. 相似文献
Cutaneous leiomyosarcomas are malignant mesenchymal tumors of smooth muscle origin and are reported occasionally in avian species. A 14-y-old male laboratory White Carneau pigeon (Columba livia) was presented for surgical excision of a cervical soft tissue mass. Ultrasonography with color flow Doppler imaging revealed multiple cavitations of mixed echogenicity within the mass and vascularization. Histologically, the dermis and subcutis were expanded by a densely cellular multinodular mass comprised of fusiform cells forming haphazardly arranged broad streams and short interwoven bundles, often surrounding blood vessels and variably sized cavitations. Neoplastic cells were strongly immunopositive for desmin and α–smooth muscle actin, and negative for pancytokeratin, S100, and von Willebrand factor. Based on histopathology and IHC findings, the cutaneous mass was diagnosed as leiomyosarcoma (LMS). The pigeon died 312 d post-operatively. Postmortem examination revealed masses infiltrating the left and right pulmonary airways and one hepatic nodule, but no regrowth at the surgical site. Histologic and IHC evaluation of the pulmonary and hepatic masses were consistent with LMS, representing metastatic foci from the primary cutaneous LMS. Our case highlights the malignant behavior and histomorphologic features of cutaneous LMS in an avian species. 相似文献
Nanotechnology applications in medicine have seen a tremendous growth in the past decade and are being employed to enhance the stability and bioavailability of lipophilic substances, such as florfenicol. This study aimed to examine the pharmacokinetic properties of the formulated oil‐in‐water florfenicol‐loaded nanoemulsion (FF‐NE). FF‐NE and florfenicol control (Nuflor®) were administered to the pigs at a dose of 20 mg/kg. Nanoemulsion formulation of florfenicol was highly influenced in vivo plasma profile. The in vivo absorption study in pigs indicated that Cmax (14.54 μg/mL) was significantly higher in FF‐NE, 3.42 times higher than the marketed formulation. In comparison with the control group, the relative bioavailability of formulated nanoemulsion was up to 134.5%. Assessment of bioequivalence using log‐transformed data showed that the 90% confidence intervals (90% CI) of Cmax and AUC0–∞ were 2.48–4.60 and 1.21–1.72, respectively. 相似文献
The study documented gross anatomical and histological differences in the reproductive organs of 28 breeding and non-breeding female guinea fowls. Peripheral progesterone and 17β-oestradiol concentrations were also compared in breeding and non-breeding hens.
In non-breeding females, all ovarian and oviducal gross anatomical features had significantly regressed. Histologically, some of the changes in a regressing oviduct include systematic changes in height and size of all epithelial cells in all regions of the duct, absence/sparse ciliation of portions of surface epithelium in the magnum, isthmian and uterine regions, general loss of cytoplasmic mass, reduction in size and degeneration of tubular glands. Mucosal folds in all regions of the oviduct except the infundibular lip were higher in breeding females.
No difference was found between the two groups in plasma progesterone concentrations. Breeding females, however, had higher peripheral oestradiol concentrations than non-breeding females. About 2 h prior to oviposition, plasma oestradiol concentrations peaked at 2.4-fold (230 pg/ml) compared with baseline concentration and plasma progesterone concentrations by nearly 9-fold (5.29 ng/ml) of baseline.
Significant regression and changes in the histological structure of the ovary and oviduct had occurred in non-breeding females, and lower peripheral oestrogen concentrations may be responsible for this phenomenon.