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The influence of the obstetrical condition on canine neonatal pulmonary functional competence 下载免费PDF全文
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Erythrocyte and Biochemical Abnormalities as Diagnostic Markers in Dogs With Hemangiosarcoma Related Hemoabdomen 下载免费PDF全文
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Ex vivo Biomechanical Comparison of V‐Loc 180® Absorbable Wound Closure Device and Standard Polyglyconate Suture for Diaphragmatic Herniorrhaphy in a Canine Model 下载免费PDF全文
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Surface Electromyography of the Vastus Lateralis,Biceps Femoris,and Gluteus Medius Muscle in Sound Dogs During Walking and Specific Physiotherapeutic Exercises 下载免费PDF全文
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RD Jolly AC Johnstone SD Williams K Zhang TW Jordan 《New Zealand veterinary journal》2013,61(5):210-217
AIM: To investigate an axonopathy of Merino sheep that caused progressive hindlimb ataxia and slight to moderate paresis, with the purpose of understanding its pathogenesis. METHODS: Tissues were fixed in buffered paraformaldehyde or paraformaldehyde and glutaraldehyde, processed into wax and epoxy resin, respectively, and examined by light and electron microscopy. Fresh frozen spinal cord and trigeminal nerve roots were subjected to homogenisation, centrifugation and two-dimensional electrophoresis. Selected protein spots were identified using matrix-assisted laser desorption ionisation (MALDI) mass spectrometry. RESULTS. By light microscopy, there were large pale foamy spheroidal axonal swellings affecting peripheral as well as central axons. By electron microscopy, these were shown to contain many membrane-bound vesicles. The main abnormalities in expressed proteins involved cytoskeletal elements and myosin heavy chain, the latter interpreted as associated with the molecular motor myosin Va. CONCLUSIONS: The disorder is the same as that described in Merinos in Australia as segmental axonopathy, and believed to have an inherited aetiology. The lesions and protein changes indicate abnormalities of the cytoskeleton, its relationship with the myelin sheath, and myosin Va molecular motor. The consequence appears to be abnormal axonal transport and inability to maintain the integrity of axons and their myelin sheaths. 相似文献