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Katie Shiels Alexandros Tsoupras Ronan Lordan Constantina Nasopoulou Ioannis Zabetakis Patrick Murray Sushanta Kumar Saha 《Marine drugs》2021,19(1)
Microalgae are at the start of the food chain, and many are known producers of a significant amount of lipids with essential fatty acids. However, the bioactivity of microalgal lipids for anti-inflammatory and antithrombotic activities have rarely been investigated. Therefore, for a sustainable source of the above bioactive lipids, the present study was undertaken. The total lipids of microalga Chlorococcum sp., isolated from the Irish coast, were fractionated into neutral-, glyco-, and phospho-lipids, and were tested in vitro for their anti-inflammatory and antithrombotic activities. All tested lipid fractions showed strong anti-platelet-activating factor (PAF) and antithrombin activities in human platelets (half maximal inhibitory concentration (IC50) values ranging ~25–200 μg of lipid) with the highest activities in glyco- and phospho-lipid fractions. The structural analysis of the bioactive lipid fraction-2 revealed the presence of specific sulfoquinovosyl diacylglycerols (SQDG) bioactive molecules and the HexCer-t36:2 (t18:1/18:1 and 18:2/18:0) cerebrosides with a phytosphingosine (4-hydrosphinganine) base, while fraction-3 contained bioactive phosphatidylcholine (PC) and phosphatidylethanolamine (PE) molecules. These novel bioactive lipids of Chlorococcum sp. with putative health benefits may indicate that marine microalgae can be a sustainable alternative source for bioactive lipids production for food supplements and nutraceutical applications. However, further studies are required towards the commercial technology pathways development and biosafety analysis for the use of the microalga. 相似文献
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Katelijn H. Lievens Ronan van Rijsbergen Frederik R. Leyns Bart J. Lambert Paul Terming Jean Swings Henk J.-P. Joos 《Pest management science》1989,27(2):141-154
A search for micro-organisms associated in high numbers with roots or leaves of corn, grapevine, chicory, soybean, sunflower, barley and sugarbeet yielded a collection of over 10 000 bacterial strains. Within this collection, antifungal strains have been selected, using direct or indirect in-vitro assays against one target fungus per crop. The target fungi were selected based on their agronomic relevance as pests. Isolates with antifungal activity were tested for their spectrum of activity against a set of phytopathogenic fungi. In 15 bacterial strains with broad-spectrum activity, belonging to the species Erwinia herbicola. Serratia plymuthica, Pseudomonas fluorescens, P. aureofaciens, P. cepacia and Bacillus subtilis, the active compounds have been identified. Bacterial strains belonging to the same taxon, but isolated from different ecological niches and/or different places in Europe, produce identical compounds. In two bacterial taxa (Erwinia and Serratia) this was reflected in a genetic conservation of the regions coding for antifungal activity. In both cases, the biosynthetic pathways proved to be genetically complex. All of the compounds identified in this study have been described previously in bacterial strains isolated from analogous or completely different ecosystems. 相似文献
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Doyle ND 《Clinical Techniques in Small Animal Practice》2004,19(3):180-191
Successful fracture management accomplishes 2 major objectives: bony union and return to functional activities. For many years, Physical Therapy has routinely helped human patients recovering from fractures reach their functional goals by helping them regain movement, flexibility, strength, coordination, and balance. Rehabilitation is now commonly provided to small animals recovering from fractures to accomplish similar goals. Knowledge of tissue healing is critical when determining the appropriate stresses to apply throughout the healing timeline. The detrimental effects of immobilization, including the potential for development of fracture disease, must be considered when formulating a Rehabilitation plan of care. Many Rehabilitation interventions are readily amenable to application by both Veterinary professionals and owners of patients. Superficial thermal modalities, passive range of motion and stretching, soft tissue massage, therapeutic ultrasound, electrical stimulation, and therapeutic exercise can ensure a more complete patient recovery. Providing owners with education regarding appropriate patient handling and home modifications allows a safer return to the home environment. Detailed written instructions for rehabilitation at home promotes owner compliance and accurate completion. 相似文献
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