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21.
22.
Evaluation of a new immunocapture test for the diagnosis of ovine brucellosis caused by Brucella melitensis 总被引:3,自引:0,他引:3
Durán-Ferrer M Mendoza J Osuna A Caporale V Lucas A León L Garrido F 《The Veterinary record》2002,151(21):629-635
A new immunocapture technique has been applied to the diagnosis of ovine brucellosis under experimental conditions. The tests were made on a serum bank derived from both young and adult ewes vaccinated conjunctivally with the Rev 1 strain at a dose of 10(8) to 10(9) colony-forming units. Adult ewes were infected experimentally two-and-a-half years after they had been vaccinated and the results were compared with an unvaccinated control group. The condition of each animal in terms of infection with Brucella melitensis was determined by clinical and bacteriological investigations. The development of the immune response was compared by the rose bengal test, the complement fixation test, the Coombs' test and the immunocapture technique for 180 days after the vaccination and for 410 days after the experimental infection, that is, the two following gestations. The results suggest that the new technique is more specific in animals vaccinated conjunctivally, regardless of their age when they were vaccinated. After the experimental infection, significantly (P < 0.05) fewer of the vaccinated sheep which were free of clinical signs and were not excreting B melitensis reacted positively to the test. 相似文献
23.
A.M.Q. Lopez J.A. Lucas 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(5):409-420
The plant defence activators acibenzolar-S-methyl (Benzo[1,2,3]thiadiazole-7-carbothioic acid-S-methyl ester, ASM), 2,6-dichloro-isonicotinic acid (DCINA), salicylic acid (SA), and dibasic potassium phosphate (K2HPO4) were tested for their ability to protect cashew (Anacardium occidentale) seeds and leaves from anthracnose disease caused by Colletotrichum gloeosporioides. No inhibition of the early stages of pathogen development was caused by concentrations equal to or lower than 1.1mM a.i. ASM, 1.2mM a.i. DCINA, 5mM SA and 50mM K2HPO4. Maximum reduction of the disease in detached leaves, without phytotoxic effects, was obtained with 0.07mM a.i. ASM and DCINA, 5mM SA, and 50mM K2HPO4, with a time interval of at least 72h between application of the activator and inoculation with the pathogen. On attached leaves, foliar sprays were slightly more efficient than soil drench treatments, with 5mM SA being the most effective treatment, while 50mM SA as well as 0.3mM a.i. ASM and DCINA caused phytotoxic effects. In field-grown plants, protection was conferred by a soil drench of concentrations as low as 12.6M a.i. ASM and DCINA and 2.6mM SA. These concentrations were not phytotoxic suggesting that plant defence activators have potential for control of anthracnose disease in the field. 相似文献
24.
A Agut X Lucas A Castro F De Membiela M Soler E Belda 《Reproduction in domestic animals》2006,41(3):247-250
An 8-year-old, entire male crossbreed dog had a 2-day history of abdominal pain and straining to urinate and defecate. A diagnosis of urolithiasis with urinary retention, and probable prostatitis was made. The dog was treated with fluids and enrofloxacin. Five days later, ultrasonography of the prostate showed two hypoechoic areas compatible with abscesses. Twelve hours later, the patient started to urinate from the rectum during micturition and urethrorectal fistula was diagnosed. The omentalization of the prostatic abscesses and castration were performed and calculi were removed. The urethrorectal fistula was treated conservatively with an indwelling urinary catheter, a low residue diet and antibiotics. The dog recovered uneventfully without recurrence of the fistula. 相似文献
25.
Localized hemibiotrophy in Colletotrichum: cytological and molecular taxonomic similarities among C. destructivum, C. linicola and C. truncatum 总被引:1,自引:1,他引:1
The infection process of hemibiotrophic isolates of Colletotrichum linicola (from flax, Linum usitatissimum ) and C . truncatum (from broad bean, Vicia faba and lentil, Lens culinaris ) was studied by light microscopy. Host surfaces were penetrated directly leading to a symptomless, biotrophic phase characterized by the elaboration of large multilobed, multiseptate, vesicular primary hyphae that were restricted to the initially infected epidermal cells. Biotrophy lasted for the first 48 h of the host-pathogen interaction and was rapidly succeeded by a necrotrophic phase during which narrow, secondary hyphae invaded the surrounding leaf tissues and water-soaked spreading lesions with sporulating, monosetate acervuli were produced on infected host surfaces. Molecular taxonomic analysis of the nucleotide sequences of the amplified D2 and ITS-2 regions of rDNA revealed very close similarities (97–99%) between these isolates and those of C . destructivum obtained from cowpea ( Vigna unguiculata ) and lucerne ( Medicago sativa ), and also of C . truncatum obtained from pea ( Pisum sativum ). This association was consistent with results from a comparative assessment of some in-planta and in-vitro morphological and growth characteristics of these hemibiotrophic fungi. It was concluded that localized hemibiotrophy is an infection strategy utilized predominantly by a closely-related group of pathogens comprising C . destructivum , C . linicola and C. truncatum , and the formation of multilobed primary hyphae restricted to the first penetrated cell might therefore be a key taxonomic character which correlates consistently with ITS sequence data. 相似文献
26.
Six calves sensitised by implanting skin from a calf were later inoculated with lymphocytes from the same calf after the calf had been infected with bovine leukosis virus (BLV). Two out of 6 calves challenged did not develop BLV antibodies and BLV was not isolated from these animals, whereas all of the 5 control calves became infected with BLV. 相似文献
27.
28.
Studies on the susceptibility of eggs of gallinaceous birds to 2,4,5-T and 2,4-D. First report
Studies were performed on a total of 3,693 eggs from Japanese quails, 1,080 eggs from pheasants and 500 hen's eggs to investigate the possible effect of 2,4-D or a mixture of 2,4-D and 2,4,5-T on the hatching rate, body weight and malformations of offsprings.Non-incubated and preincubated (3 days) eggs were sprayed with up to 10 times the amount of herbicides normaley used. The eggs serving as controls were treated with tap water.As compared with the controls, the herbicides did not affect hatching rate, body weight or the number of malformations. 相似文献
29.
In this study we determined soil moisture storage, evapotranspiration (ET) and light interception in an agroforestry trial consisting of pine trees grown over (1) control (bare ground), (2) ryegrass/clovers (Lolium perene/Trifolium spp.), (3) lucerne (Medicago sativa), and (4) ryegrass only during the third growing season between 1992 and 1993. The results show that:
相似文献
1. | In the period when rainfall was frequent and exceeded the evaporative demand (Epot), ET and depletion of soil moisture were not affected by the ground cover treatments. During summer when rainfall was less frequent, but with moisture readily available in the soil profile, ET was associated with plant canopy, and was significantly higher for the pasture ground covers than for the control. Therefore, the more rapid growth by lucerne caused higher ET in this ground cover than in the ryegrass/clovers ground cover in which the pasture was slow growing. At the end of the study period, total ET was in the following order: lucerne (757 mm) > ryegrass/clovers (729 mm) > Control (618 mm). |
2. | ET was dominated by pasture transpiration (Ep) during most of the growing season, but by tree transpiration (Et) in winter when large parts of the pasture canopy was shaded. Ep was always at least 16% higher for lucerne than for ryegrass/clovers species as a result of a greater radiation intercepted by the former. |
3. | Fraction of incoming radiation intercepted by the tree crowns was in the following order: control > ryegrass > ryegrass/clovers > lucerne. At the end of the one-year period, fraction of intercepted radiation was 140% greater for control than for lucerne ground cover. |
4. | The control produced the largest tree crowns, which were almost twice the tree crowns in the lucerne ground cover which produced the smallest trees. Accordingly, the trees in the control intercepted more radiation and rainfall, with the former being lost to evaporation, than the trees in the pasture. |
5. | The fractions of radiation intercepted and ET accounted for by the trees and pastures were associated with the proportion of the plot area they occupied. |
30.
We measured oxygen isotope ratios (delta18O) of xylem sap, phloem sap, leaves, wood and bark of Eucalyptus globulus Labill. growing in southwestern Australia. Carbon isotope ratios (delta13C) were measured in the dry matter of phloem sap, leaves and wood. Results were used to test several aspects of a mechanistic model of 18O enrichment and provided insights into post-photosynthetic variations in dry matter delta13C. Xylem water delta18O varied little within the tree crown, whereas variation at the landscape-level was more pronounced, with plantations near the coast being enriched by up to 3 per thousand compared with plantations less than 100 km inland. Phloem water was significantly enriched in 18O compared with xylem water in two of three sampling campaigns; mean enrichments were 0.5 and 0.8 per thousand. Phloem sap sugars exported from E. globulus leaves closely reflected observed leaf water enrichment when diurnal variation in photosynthesis was taken into account. Photosynthetic rates were higher in the morning than in the afternoon, whereas leaf water 18O enrichment increased to maximum values in the afternoon. A non-steady-state model of leaf water 18O enrichment accurately predicted observed values through a full diel cycle. Mean estimates of the proportion of organic oxygen effectively exchanging with xylem water during cellulose synthesis were close to 0.40 for both leaves and wood. Carbon isotope ratios of nascent xylem tissues did not differ from those of phloem sap sugars collected concurrently, whereas nascent leaf tissues were depleted in 13C by 2 per thousand compared with phloem sap sugars, suggesting that, in E. globulus, 13C enrichment of sink tissues compared with source leaves does not result from an enriching process within the sink tissue. 相似文献