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81.
Rat bite fever (RBF) is a bacterial zoonosis for which two causal bacterial species have been identified: Streptobacillis moniliformis and Spirillum minus. Haverhill fever (HF) is a form of S. moniliformis infection believed to develop after ingestion of contaminated food or water. 相似文献
82.
Ofri R Lambrou GN Allgoewer I Graenitz U Pena TM Spiess BM Latour E 《Veterinary journal (London, England : 1997)》2009,179(1):70-77
The aim of this study was to evaluate the efficacy of pimecrolimus oil-based eye drops in alleviating the clinical signs of keratoconjunctivitis sicca (KCS) in dogs and to compare the efficacy with that of cyclosporine A (CsA) ointment. An open-label, multicenter study enrolling 44 dogs previously untreated with CsA was conducted. Dogs were randomly assigned to a treatment group and medicated twice daily for 8 weeks. After that time the mean increase (+/-SEM) in the Schirmer tear test was 9.2+/-1.6 mm/min in the pimecrolimus group and 5.8+/-1.1 mm/min in the CsA group (P=0.085). The improvement in clinical signs of inflammation in eyes treated with pimecrolimus was significantly greater than in eyes treated with CsA (P=0.02). The results show that 1% pimecrolimus oily eye drops are as safe as and more effective than CsA ointment in controlling KCS in dogs. 相似文献
83.
Slocombe JO 《Veterinary parasitology》2006,136(2):127-135
Aims of this study with 43 equids naturally infected with Anoplocephala perfoliata in two dose titration trials were to document (i) the usefulness of a critical test with a 48 h treatment to necropsy period, (ii) efficacy of an oral paste of 0.5-2.0 mg praziquantel/kg body weight, and (iii) when after treatment would fecal egg counts provide best estimates of the tapeworm's prevalence in a herd. All feces passed by an equid after treatment and collected in successive 12 h batches were examined for tapeworms. At necropsy, tapeworms were identified as attached to the mucosa or unattached. Tapeworms were examined with a stereoscope and identified as normal or abnormal. Fecal samples were taken for egg counts at treatment and at 6 h intervals thereafter. In 32 of 36 treated equids, efficacy was 100% and mean efficacies for 0.5, 1.0, 1.5 and 2.0 mg praziquantel/kg body weight were 85.5, 99.7, 100 and 100%, respectively. Two equids treated with 0.5 mg praziquantel/kg body weight had low efficacies (5.4 and 38.1%) and normal tapeworms were found attached in the ventral colon and in one equid also in the dorsal colon. In seven untreated equids, tapeworms were primarily in the cecum with 21.3% detached. "Major fragments" or worms without a scolex but otherwise nearly a complete worm were 20.5% of the number of intact worms; they were not included in the efficacy analysis but should be. If the two equids with low efficacy were eliminated and if the number of all tapeworms and major fragments are combined less than 0.5% were in feces within 12 h of treatment, about 20% were in the 12-24 h period, 42% in 24-36h, 24% in 36-48 h and 13.5% in the equids at necropsy. One horse passed all its tapeworms in 24 h. This 48 h test when compared with a 24 h one with no examination of feces was more efficient in use of trial animals and reduced underestimation and overestimation of efficacy. However, a protocol similar to the 48 h test but with a 24 or 36 h post-treatment period should be investigated. The mean egg count peaked 18-24 h after treatment and samples taken at that time would provide the best estimate of prevalence. The Cornell-Wisconsin centrifugal flotation technique had a specificity of 100% and at 18 and 24 h its sensitivity was 94%. A brief discussion on critical and controlled tests for assessing efficacy of an anthelmintic for A. perfoliata is presented. 相似文献
84.
Shaw DE Maragakis P Lindorff-Larsen K Piana S Dror RO Eastwood MP Bank JA Jumper JM Salmon JK Shan Y Wriggers W 《Science (New York, N.Y.)》2010,330(6002):341-346
Molecular dynamics (MD) simulations are widely used to study protein motions at an atomic level of detail, but they have been limited to time scales shorter than those of many biologically critical conformational changes. We examined two fundamental processes in protein dynamics--protein folding and conformational change within the folded state--by means of extremely long all-atom MD simulations conducted on a special-purpose machine. Equilibrium simulations of a WW protein domain captured multiple folding and unfolding events that consistently follow a well-defined folding pathway; separate simulations of the protein's constituent substructures shed light on possible determinants of this pathway. A 1-millisecond simulation of the folded protein BPTI reveals a small number of structurally distinct conformational states whose reversible interconversion is slower than local relaxations within those states by a factor of more than 1000. 相似文献
85.
Kanner J Gorelik S Roman S Kohen R 《Journal of agricultural and food chemistry》2012,60(36):8790-8796
Recent studies dramatically showed that the removal of circulating modified low-density lipoprotein (LDL) results in complete prevention of atherosclerosis. The gastrointestinal tract is constantly exposed to food, some of it containing oxidized compounds. Lipid oxidation in the stomach was demonstrated by ingesting heated red meat in rats. Red wine polyphenols added to the rats' meat diet prevented lipid peroxidation in the stomach and absorption of malondialdehyde (MDA) in rat plasma. In humans, postprandial plasma MDA levels rose by 3-fold after a meal of red meat cutlets. MDA derived from meat consumption caused postprandial plasma LDL modification in human. The levels of plasma MDA showed a 75% reduction by consumption of red wine polyphenols during the meat meal. Locating the main biological site of action of polyphenols in the stomach led to a revision in the understanding of how antioxidants work in vivo and may help to elucidate the mechanism involved in the protective effects of polyphenols in human health. 相似文献
86.
Proaño-Pérez F Benitez-Ortiz W Desmecht D Coral M Ortiz J Ron L Portaels F Rigouts L Linden A 《Preventive veterinary medicine》2011,101(1-2):65-72
Veterinary inspection in slaughterhouses allows for the detection of macroscopic lesions reminiscent of bovine tuberculosis, but the presence of Mycobacterium bovis must be confirmed by laboratory methods. This study aimed at comparing the performances of the standard diagnostic tools used to identify M. bovis in tissue specimens sampled from suspicious animals. During a two years period, 1390 cattle were inspected at the Machachi abattoir in the Mejia canton - Ecuador. A total of 33 animals with granulomatous lesions were detected, representing 2.33% (16/687) and 2.42% (17/703) animals examined in 2007 and 2008, respectively. Ninety-four tissue specimens were sampled and screened for the presence of mycobacteria. Acid-fast bacilli were identified in one third of the suspicious cattle (11/33) and suggestive microscopic lesions in 27.3% (9/33) of the samples examined by direct microscopy and histopathology, respectively. Culturing on Stonebrink medium and 16S-rRNA-based polymerase chain reaction (PCR) yielded 36.4% (12/33) and 27.3% (9/33) of positives, respectively. Compared to culture, other diagnostic procedures displayed a lower sensitivity, with 56.5% for PCR, and 43.5% for direct microscopy and histopathology; however, the specificity was higher (94.4% for PCR and microscopy, and 97.2% for histopathology). We conclude that reliable post-mortem laboratory testing either requires the combination of a set of available diagnostic tools or necessitates the development of improved new-generation tools with better sensitivity and specificity characteristics. 相似文献
87.
Gea A Stringano E Brown RH Mueller-Harvey I 《Journal of agricultural and food chemistry》2011,59(2):495-503
A rapid thiolytic degradation and cleanup procedure was developed for analyzing tannins directly in chlorophyll-containing sainfoin ( Onobrychis viciifolia ) plants. The technique proved suitable for complex tannin mixtures containing catechin, epicatechin, gallocatechin, and epigallocatechin flavan-3-ol units. The reaction time was standardized at 60 min to minimize the loss of structural information as a result of epimerization and degradation of terminal flavan-3-ol units. The results were evaluated by separate analysis of extractable and unextractable tannins, which accounted for 63.6-113.7% of the in situ plant tannins. It is of note that 70% aqueous acetone extracted tannins with a lower mean degree of polymerization (mDP) than was found for tannins analyzed in situ. Extractable tannins had between 4 and 29 lower mDP values. The method was validated by comparing results from individual and mixed sample sets. The tannin composition of different sainfoin accessions covered a range of mDP values from 16 to 83, procyanidin/prodelphinidin (PC/PD) ratios from 19.2/80.8 to 45.6/54.4, and cis/trans ratios from 74.1/25.9 to 88.0/12.0. This is the first high-throughput screening method that is suitable for analyzing condensed tannin contents and structural composition directly in green plant tissue. 相似文献
88.
Ron Rainey Philip G. Crandall Corliss A. O’Bryan Steven C. Ricke Sean Pendleton Steve Seideman 《Journal Of Agricultural & Food Information》2013,14(2):141-153
Consumers embrace local food systems as an alternative to the global corporate model, and nearly 75% of consumers in the United States purchase organic foods occasionally. To assess consumers’ knowledge of locally grown organic foods, surveys were administered at three metropolitan farmers’ markets in Little Rock, Hot Springs, and Texarkana, Arkansas. Consumers with Bachelor's, Associate's, or technical degrees accounted for almost half of the people surveyed. Seventy-one percent believed organic foods were safer than conventional foods. Three times as many consumers were concerned about harmful bacteria in conventional foods than in organic. The number one reason for purchasing was to support local farmers. 相似文献
89.
Melon collapse caused by the heat-tolerant soil-borne fungus Monosporascus cannonballus is not controlled by current solarization technology applied to large soil volumes because the temperatures achieved are not high enough to kill the pathogen's ascospores. The efficacy of improved solarization achieved by mulching a shallow layer of growth medium in containers was studied in terms of disease control and yield increase over two growing seasons. Compared to solarization of a regular container, solarization of a shallow layer (5–6 cm) of growth medium resulted in a 3–5°C elevation in maximal temperature at a depth of 3 cm, and in a 7–10°C increases at the bottom of the shallow layer. This led to a higher disease reduction and higher yields in the 1996 experiment. In 1997, both methods of solarization were highly effective. Yield was highly correlated with disease reduction in both experiments. In the 1997 experiment, ascospore viability declined faster in the shallow container treatment than in the regular container. This study demonstrates the potential of an additional approach for improving solarization of containerized plant growth medium and improving its reliability, with the aim of controlling heat-tolerant pathogens. 相似文献
90.
John G. Howieson Robert J. Harrison Ron J. Yates Belinda Hackney Angelo Loi Brad J. Nutt 《Grass and Forage Science》2021,76(1):82-92
The patterns and extent of hard seed breakdown of dormant seeds and pods following burial in the soil in February (summer) varied for up to 19 accessions of 12 species of annual forage legumes. The experiments, at six sites across southern Australia, were designed to identify legumes whose patterns of hard seed breakdown would suit them to agronomic evaluation in a summer sowing research program. For a subset of accessions, sufficient hard seed breakdown was demonstrated between February and April to allow a high proportion of seed to germinate in late autumn—a desirable pattern for summer sowing. Although there was a large G x E interaction, some cultivars of Ornithopus sativus Brot., O. compressus L. and Trifolium spumosum L. had a pattern that was reproducible at all sites. The experiments also identified accessions of Biserrula pelecinus L. and T. glanduliferum Boiss., which had the desirable pattern in regions of south‐eastern Australia, but not Western Australia. A pragmatic approach for selection of forage legume accessions in situ for evaluation within a summer sowing program was demonstrated. 相似文献