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161.
Johnson EM Allen KE Breshears MA Panciera RJ Little SE Ewing SA 《Veterinary parasitology》2008,151(2-4):164-169
Laboratory-raised cotton rats (Sigmodon hispidus), outbred white mice (Mus musculus), and C57BL/6J-Lystbg-J/J mice (M. musculus) that were administered approximately 50 sporulated oocysts of Hepatozoon americanum (AF176836) by gavage developed inflammatory lesions containing parasitic cystozoites in cardiac and skeletal muscle, kidney, and lung. Sprague-Dawley rats (Rattus norvegicus) similarly exposed showed no evidence of infection. Cystozoites were first detected by histopathologic examination four weeks after exposure to oocysts. Globular, PAS-positive material accumulated around the cystozoites as the duration of infection lengthened. Nested PCR analysis of tissues collected 16 weeks post-exposure was positive for the 18S rRNA Hepatozoon sp. gene and the DNA sequence of the fragment amplified was 99.6% and 99.8% identical to H. americanum sequences previously reported from naturally-infected dogs (AF176836 and AY864676, respectively). Merogonous and gamontogonous stages of the parasite were not detected in any of the cystozoite-infected rodents. 相似文献
162.
The addition of the organosilicone surfactant ‘Silwet L77’ at 1-5 ml litre?1 to formulated glyphosate gave complete surface wetting on application to the adaxial leaf surface of perennial ryegrass (Lolium perenne L.). The wetting characteristics of the solution were associated with rapid foliar uptake and near maximal uptake in 3 h compared to greater than 5 h in the absence of ‘Silwet L77’. Evidence is presented showing that solutions containing ‘Silwet L77’ rapidly infiltrate stomata. Rapid uptake did not occur after application to the astomatous abaxial surface of perennial ryegrass leaves. The rapid rate of glyphosate uptake reduced the critical rainfall period to 2 h or less, compared to up to 10 h in the absence of ‘Silwet L77’. The use of ‘Silwet L77’ has major practical implications for the use of glyphosate in regions with unpredictable rainfall or high rainfall frequency. 相似文献
163.
Marlina?D.?NasutionEmail author Cavell?Brownie Kenneth?H.?Pollock Roger?A.?Powell 《Journal of Agricultural, Biological & Environmental Statistics》2004,9(1):27-41
Models are described for the joint analysis of live-trapping and radio telemetry data from a study on black bears (Ursus americanus) in which all animals received ear tags and a subset also received radio tags. Concerns about bias in survival estimates
led to investigation of identifiability and estimator precision for a series of models that allowed differenttelemetry relocation
rates for living and dead animals, in addition to emigration and seasonal variation in survival. Identifiability was determined
by showing that the expected information matrix was nonsingular. Models with fidelity constant across time, and with the same
degree of time specificity for survival rates and relocation rates for dead animals, were determined to be nonidentifiable.
More general models, with a greater degree of time specificity for survival rates, were near-singular, and estimators under
these near-singular models had poor precision. Analysis of data from the study on black bears illustrated that estimates of
survival have poor precision when relocation rates are estimated separately for live and dead animals. It is recommended that
the effort expended to relocate both living and dead animals be consistently high in each telemetry survey, so that relocation
rates will be high and constant across time and mortality status. 相似文献
164.
Rice CC Kruger JM Venta PJ Vilnis A Maas KA Dulin JA Maes RK 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(3):293-302
Feline caliciviruses (FCVs) are potential etiologic agents in feline idiopathic lower urinary tract disease (I-LUTD). By means of a modified virus isolation method, we examined urine obtained from 28 male and female cats with nonobstructive I-LUTD, 12 male cats with obstructive I-LUTD, and 18 clinically healthy male and female cats. All cats had been routinely vaccinated for FCV. Two FCVs were isolated; I (FCV-U1) from a female cat with nonobstructive I-LUTD, and another (FCV-U2) from a male cat with obstructive I-LUTD. To determine the genetic relationship of FCV-U1 and FCV-U2 to other FCVs. capsid protein gene RNA was reverse transcribed into cDNA, amplified, and sequenced. Multiple amino acid sequence alignments and phylogenetic trees were constructed for the entire capsid protein, hypervariable region E, and the more conserved (nonhypervariable) regions A, B, D, and F. When compared to 23 other FCV isolates with known biotypes, the overall amino acid sequence identity of the capsid protein of FCV-U1 and FCV-U2 ranged from 83 to 96%; identity of hypervariable regions C and E ranged from 58 to 85%. Phylogenetically, FCV-U1 clearly separated from other FCV strains in phenograms based on nonhypervariable regions. In contrast, FCV-U2 consistently segregated with the Urbana strain in all phenograms. Clustering of isolates by geographic origin was most apparent in phenograms based on nonhypervariable regions. No clustering of isolates by biotype was apparent in any phenograms. Our results indicate that FCV-UI and FCV-U2 are genetically distinct from other known vaccine and field strains of FCV. 相似文献
165.
Labruna MB McBride JW Camargo LM Aguiar DM Yabsley MJ Davidson WR Stromdahl EY Williamson PC Stich RW Long SW Camargo EP Walker DH 《Veterinary parasitology》2007,143(2):189-195
A molecular epidemiologic investigation in two Brazilian states (Rondônia and São Paulo) was undertaken to determine if Ehrlichia species responsible for human and animal ehrlichioses in North America could be found in Brazilian vectors, potential natural mammalian reservoirs and febrile human patients with a tick bite history. Samples, including 376 ticks comprising 9 Amblyomma species, 29 capybara (Hydrochaeris hydrochaeris) spleens, 5 canine blood, and 75 human blood samples from febrile patients with history of tick bites were tested by a real-time PCR assay targeting a fragment of the Ehrlichia dsb gene. Ehrlichia DNA was not detected in any tick, capybara or human samples. In contrast, 4 out of 5 dogs contained Ehrlichia canis DNA in their blood, which were sequenced, representing the first report of E. canis infecting dogs in the Amazon region of Brazil. Further studies are needed to evaluate the presence of other agents of human and animal ehrlichioses in Brazil. 相似文献
166.
167.
Timo J. Ruokonen Mikko Kiljunen Jaakko Erkinaro Panu Orell Olli Sivonen Eero Vestola Roger I. Jones 《Ecology of Freshwater Fish》2019,28(1):53-61
We estimated the proportions of anadromous and freshwater‐resident brown trout (Salmo trutta) in different parts of the subarctic River Näätämöjoki/Neidenelva system (Finland and Norway) using carbon, nitrogen and hydrogen stable isotope analyses of archived scales as identifiers of migration strategy. Our results showed that carbon stable isotope values were the best predictor of migration strategy. Most individuals fell into two clearly distinct groups representing anadromous (47%) or freshwater‐resident (42%) individuals, but some fish had intermediate carbon values suggesting repeated movement between freshwater and the sea. The proportion of anadromous individuals decreased steadily with distance from the sea forming a spatial continuum in migration strategies which is probably maintained by the combination of several factors such as divergent availability of food resources, variable migration costs and genetic differences. These within‐catchment differences in migration strategies should be taken into account in fisheries management practices. 相似文献
168.
Purpose
The aims of this paper are to outline the state of knowledge with regard to the chemistry of soil organic matter (SOM) prior to 1950; then, to review and evaluate the contributions made by Frank Stevenson during Stevenson’s research period (1950–1994); and subsequently to outline advances that are being made in the modern era.Progress in the Stevenson period
Frank Stevenson’s research career began in the middle of the twentieth century when a number of techniques of colloid chemistry were available to him, but relatively few of the recently developed instrumental techniques and other procedures of analytical organic chemistry that have enabled significant advances to be made in the chemistry and properties of SOM components.The contributions that Frank Stevenson has made to the chemistry of SOM and of humic substances (HSs) are an integral part of his book (first and second editions) Humus Chemistry: Genesis, Composition, Reactions. The validity of the terms humus and HSs are being questioned as legitimate terms that describe definable components, and SOM is being viewed as a ‘continuum of progressively decomposing organic compounds’. The legitimacy of isolating the organic matter components from their native soil environment is questioned. Those who pose such questions would do well to consider how progress could have been made in the vital life sciences areas of, for example, proteomics and genomics, without the isolation of the relevant cellular components. We recognise the importance of clear and rigorous definitions of HS components and stress the need to isolate these components from the SOM matrix as a prerequisite to the study of the composition, structure and reactivity of these components. We disagree with proposals or suggestions that do not recognise HSs as a scientific entity, and we feel sure that Frank Stevenson would have supported this stance. Various studies of SOM and of HSs have taken place over the centuries, but progress was slow because the tools required to study such complex systems were not available. Frank Stevenson’s research involved many areas of humic chemistry, and his major advances were in aspects of functionality and in the interactions of humic functional groups with metals and to a lesser extent with anthropogenic organic chemicals. His studies of nitrogen and of ammonia in relation to organic matter also had a very great impact.Progress in the modern era
Frank Stevenson can be said to have provided the stimulation that enabled beginners and established scientists to obtain a good grasp of the fundamentals of SOM and the humic sciences. His scientific contributions have catalysed many of the significant advances that have been made in the field since he retired. In the final section, some of the advances that have been made using modern analytical techniques are addressed and some of the controversial topics that have recently arisen are discussed.169.
Despite the considerable research efforts invested over the years to measure the fitness costs of herbicide resistance, these have rarely been used to inform a predictive theory about the fate of resistance once the herbicide is discontinued. One reason for this may be the reductive focus on relative fitness of two genotypes as a single measure of differential performance. Although the extent of variation in relative fitness between resistant and susceptible plants has not been assessed consistently, we know enough about plant physiology and ecology not to reduce it to a single fixed value. Research must therefore consider carefully the relevance of the experimental environment, the life stage and the choice of metric when measuring fitness‐related traits. The reason most often given for measuring the cost of resistance, prediction of the impacts of management options on population dynamics, cannot be addressed using arbitrary components of fitness or a fixed value of relative fitness. To inform management options, the measurement of traits that capture the relevant processes and the main causes of their variation are required. With an emphasis on the benefit of field experiments measured over multiple time points and seasons, we highlight examples of studies that have made significant advances in this direction. © 2017 Society of Chemical Industry 相似文献