The correlation of potassium content and moisture in bovine liver samples analyzed for trace mineral concentrations.

Beef cattle in the United States are often found to be deficient in essential trace minerals such as copper and zinc. Established reference ranges for mineral concentrations exist and usually designate a concentration as adequate, marginal, deficient, or excessive. This research investigates a new method of interpreting detected elemental concentrations in bovine liver that will add confidence to the final diagnosis. This is based on the hypothesis that a correlation exists between potassium concentration and moisture in a bovine liver sample. This relationship between potassium and moisture content enables the diagnostician to more accurately predict mineral concentrations and wet weight regardless of sample moisture loss. Correlations were found between potassium content and percentage of moisture in experimental samples, clinical biopsies, and a validation study, to a statistical significance of P < 0.001. Experimental samples had a correlation coefficient of R2 = 0.95 and the mathematical relationship y = 2513.2x(-1.0662). Clinical biopsies had a correlation of R2 = 0.83 and the mathematical relationship y = 2203.4x(-0.991). The validation study had a correlation of R2 = 0.55 and a mathematical relationship y = 2321.4x(-0.952). An exponent of -1 is predicted by conservation of potassium mass. These findings have practical significance in maintaining and improving cattle growth, health, reproduction, and food safety.     

Elevated carbon dioxide increases contents of antioxidant compounds in field-grown strawberries

The effects of elevated CO2 concentrations on the antioxidant capacity and flavonoid content in strawberry fruit (Fragaria x ananassa Duch.) were studied under field conditions. Increased CO(2) (300 and 600 micromol mol(-1) above ambient) concentrations resulted in increases in ascorbic acid (AsA), glutathione (GSH), and ratios of AsA to dehydroascorbic acid (DHAsA) and GSH to oxidized glutathione (GSSG), and a decrease in DHAsA in strawberry fruit. High anthocyanin and phenolic content were also found in fruit of CO(2) treated plants. Growing strawberry plants under CO(2) enrichment conditions significantly enhanced fruit p-coumaroylglucose, dihydroflavonol, quercetin 3-glucoside, quercetin 3-glucuronide, and kaempferol 3-glucoside contents, as well as cyanidin 3-glucoside, pelargonidin 3-glucoside, and pelargonidin 3-glucoside-succinate content. Fruit of strawberry plants grown in the CO(2) enrichment conditions also had high oxygen radical absorbance activity against ROO(*), O(2)(*-), H(2)O(2), OH(*), and (1)O(2) radicals.     

Rubber production of salt-stressed guayule at various plant populations

Summary The hypothesis that increasing the plant population of guayule (Parthenium argentatum) to compensate for the reduced plant canopy size caused by soil salinity coupled with an anticipated enchancement of rubber production under the moderate environmental stress imposed by salinity was tested in a field plot experiment in the Imperial Valley of California. Irrigation waters having electrical conductivities (EG _i ) of 1.2, 3.2, 6.5, and 9.4 dS/m were applied for 4 years to plots having plant populations of 28,000, 56,000, and 84,000 plants per hectare. The influence of salinity on rubber and resin production was independent of plant population. The salt tolerance threshold, maximum average salinity level of the root zone measured as the electrical conductivity of saturated soil extracts ( ) without yield reduction, was 7.5 dS/m; beyond this threshold, rubber production was reduced 6.1% per unit increase of soil salinity. The salinity values were averaged through the root zone from planting to harvest. The average rubber content — 7.9% — was altered little by treatment or harvest age for 2- to 4-year-old plants. Resin content averaged 8.4% but increased salinity and increased plant population increased the resin content slightly in some cases. Dry matter production of shoots for the nonsaline treatment was 259 kg/ha/month for pollarded (clipped) shoots after 31 months, 203 kg/ha/month for shoots harvested after 43 months, and 401 kg/ha/month for the 24-month period after pollarding. Combining the shoot mass after 31 and 55 months gave an average growth rate of 321 kg/ha, supporting the recommendation for pollarding. Monthly growth rates for the lowest salt treatment (3.2 dS/m) were about 10% less than for the nonsaline treatment (1.2 dS/m). The hypothesis tested was proven to be false because neither increased salinity nor increased plant population increased rubber production.     

The control of lymphoid leukosis in a flock White Plymouth Rock chickens

Summary

Lymphoid leukosis (LL) was successfully controlled in a commercial basic breeding line of White Plymouth Rock chickens. The control method has been developed for breeder flocks and consists of three elements: - In the flock under study, homogenates of embryos from all eggs collected during a number of I4‐day periods are tested for the presence of LL viruses.

- Only eggs from hens that have been shown not to shed virus in their eggs are used for the production of progeny. The offspring are reared in isolation during the first two months of life, at which time the age‐related resistance against tumour formation by LL viruses appears to be sufficiently developed.

- The chickens are subsequently inoculated intramuscularly with LL viruses of subgroups A and B transferred to a conventional chicken house.

The vaccination raises a solid immunity to horizontal LL virus exposure and, due to the age‐related resistance, tumour formation does not follow.

No excretion of LL viruses could be detected in three generations of White Plymouth Rock chickens to which the three elements of the control procedure were applied. Clinical disease was not observed in any of the chickens under notice.