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91.
Summary Genetic variability in the apple [Malus] fruit disorder bitter pit and fruit calcium concentrations ([Ca]) was determined in 25 seedling families at each of two sites from 1999 to 2000 and again for one site, in 2001. Most trees were free of pit or had low pit incidence, which could be approximated by an over-dispersed binomial distribution. A genetic component to external and internal pit incidence was found across both sites and all years. This effect was irrespective of the use of several cropping factors, average fruit weight or fruit mineral concentrations as covariates in the analyses. Year and/or site always had substantial effects on pit incidence. Interactions of genetic effects with site or year were also detected with large differences in mean pit incidence between sites or years for some families but not others. No relationship was found between mean family pit incidence and mean family [Ca] or mean family harvest date. However fruit [Ca] and harvest date were usually important predictors of within-family variation in pit incidence. For any one seedling, family had the largest effect on bitter pit incidence followed by site and [Ca] and the smallest effect was that of harvest date. [Ca] showed a strong genetic component and estimated family means of [Ca] were consistent at different sites and years. These results suggest that susceptibility of apple genotypes to bitter pit in breeding programmes may be best assessed through screening across multiple sites and seasons. Fruit [Ca] might be useful as an indirect selection index for bitter pit within, but not among families. 相似文献
92.
Moytri RoyChowdhury Yulin Jia Aaron Jackson Melissa H. Jia Robert Fjellstrom Richard D. Cartwright 《Euphytica》2012,184(1):35-46
The Pi-z gene in rice confers resistance to a wide range of races of the rice blast fungus, Magnaporthe oryzae. The objective of this study was to characterize Pi-z in 111 rice germplasm accessions using DNA markers and pathogenicity assays. The existence of Pi-z in rice germplasm was detected by using four simple sequence repeat (SSR) markers (RM527, AP4791, AP5659-1, AP5659-5) closely
linked to Pi-z, and was verified using pathogenicity assays with an avirulent strain (IE1k) and two virulent races (IB33 and IB49). Among
111 germplasm accessions evaluated, 73 were found to contain the Pi-z gene using both SSR markers and pathogenicity assays. The remaining 38 germplasm accessions were found to be inconsistent
in their responses to the blast races IB33, IEIk and IB49 with expected SSR marker alleles, suggesting the presence of unexpected
SSR alleles and additional R gene(s). These characterized germplasm can be used for genetic studies and marker-assisted breeding for improving blast resistance
in rice. 相似文献
93.
94.
Jeanne L. Nel Dirk J. Roux Robin Abell Peter J. Ashton Richard M. Cowling Jonathan V. Higgins Michele Thieme Joshua H. Viers 《水产资源保护:海洋与淡水生态系统》2009,19(4):474-485
- 1. Freshwater ecosystems and their associated biota are among the most endangered in the world. This, combined with escalating human pressure on water resources, demands that urgent measures be taken to conserve freshwater ecosystems and the services they provide. Systematic conservation planning provides a strategic and scientifically defensible framework for doing this.
- 2. Pioneered in the terrestrial realm, there has been some scepticism associated with the applicability of systematic approaches to freshwater conservation planning. Recent studies, however, indicate that it is possible to apply overarching systematic conservation planning goals to the freshwater realm although the specific methods for achieving these will differ, particularly in relation to the strong connectivity inherent to most freshwater systems.
- 3. Progress has been made in establishing surrogates that depict freshwater biodiversity and ecological integrity, developing complementarity‐based algorithms that incorporate directional connectivity, and designing freshwater conservation area networks that take cognizance of both connectivity and implementation practicalities.
- 4. Key research priorities include increased impetus on planning for non‐riverine freshwater systems; evaluating the effectiveness of freshwater biodiversity surrogates; establishing scientifically defensible conservation targets; developing complementarity‐based algorithms that simultaneously consider connectivity issues for both lentic and lotic water bodies; developing integrated conservation plans across freshwater, terrestrial and marine realms; incorporating uncertainty and dynamic threats into freshwater conservation planning; collection and collation of scale‐appropriate primary data; and building an evidence‐base to support improved implementation of freshwater conservation plans.
95.
Conservationists, managers, and land planners are faced with the difficult task of balancing many issues regarding humans
impacts on natural systems. Many of these potential impacts arise from local-scale and landscape-scale changes, but such changes
often covary, which makes it difficult to isolate and compare independent effects arising from humans. We partition multi-scale
impacts on riparian forest bird distribution in 105 patches along approximately 500 km of the Madison and Missouri Rivers,
Montana, USA. To do so, we coupled environmental information from local (within-patch), patch, and landscape scales reflecting
potential human impacts from grazing, invasive plant species, habitat loss and fragmentation, and human development with the
distribution of 28 terrestrial breeding bird species in 2004 and 2005. Variation partitioning of the influence of different
spatial scales suggested that local-scale vegetation gradients explained more unique variation in bird distribution than did
information from patch and landscape scales. Partitioning potential human impacts revealed, however, that riparian habitat
loss and fragmentation at the patch and landscape scales explained more unique variation than did local disturbances or landscape-scale
development (i.e., building density in the surrounding landscape). When distribution was correlated with human disturbance,
local-scale disturbance had more consistent impacts than other scales, with species showing consistent negative correlations
with grazing but positive correlations with invasives. We conclude that while local vegetation structure best explains bird
distribution, managers concerned with ongoing human influences in this system need to focus more on mitigating the effects
of large-scale disturbances than on more local land use issues.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
96.
Timbermont L Lanckriet A Gholamiandehkordi AR Pasmans F Martel A Haesebrouck F Ducatelle R Van Immerseel F 《Comparative immunology, microbiology and infectious diseases》2009,32(6):503-512
Since the ban on growth-promoting antibiotics in animal feed in the European Union, necrotic enteritis has become a major cause of mortality in broiler chickens. Despite the importance of the disease, the pathogenesis is still not completely understood. In the current study, Clostridium perfringens strains isolated from healthy flocks and isolates from outbreaks of necrotic enteritis were evaluated for the ability to cause gut necrosis in an intestinal loop model in laying hens and in an experimental infection model in broilers. High, intermediate and low alpha toxin producing strains were chosen from each isolation source. Only the isolates from field outbreaks induced necrotic gut lesions, independent of the amount of alpha toxin produced in vitro. It was also shown that alpha toxin producing isolates from calf hemorrhagic enteritis cases were not able to induce necrotic enteritis in poultry. These results suggest the presence of host specific virulence factors in C. perfringens strains, isolated from chickens with intestinal necrotic enteritis lesions. 相似文献
97.
Marisa N. C. Litz Jessica A. Miller Richard D. Brodeur Elizabeth A. Daly Laurie A. Weitkamp Adam G. Hansen Andrew M. Claiborne 《Fisheries Oceanography》2019,28(3):273-290
Variation in prey quantity and quality can influence growth and survival of marine predators, including anadromous fish that migrate from freshwater systems. The objective of this study was to examine the energy dynamics of subyearling Chinook salmon (Oncorhynchus tshawytscha) following freshwater emigration. To address this objective, a population of Chinook salmon and their marine prey were repeatedly sampled from June to September over 2 years in coastal waters off Oregon and Washington. Subyearlings from the same population were also reared under laboratory conditions. Using a bioenergetics model evaluated in the laboratory, we found that growth rate variability in the field was associated more with differences in northern anchovy (Engraulis mordax) consumption and less with variation in diet energy density or ocean temperature. Highest growth rates (2.43–3.22% body weight/day) occurred in months when anchovy biomass peaked, and the timing of peak anchovy biomass varied by year. Our results support a general pattern among subyearling Chinook salmon occurring from Alaska to California that feeding rates contribute most to growth rate variability during early marine residence, although dominant prey types can differ seasonally, annually, or by ecosystem. In the northern California Current, faster growth appears to be associated with the availability of age‐0 anchovy. Identifying factors that influence the seasonal development of the prey field and regulate prey quantity and quality will improve understanding of salmon growth and survival during early marine residence. 相似文献
98.
Agnes Agunos Sheryl P. Gow David F. Lger Logan Flockhart Danielle Daignault Andrea Desruisseau Erin Zabek Frank Pollari Richard J. Reid-Smith 《Canadian journal of veterinary research》2021,85(1):27
Eggs are important to the diet of Canadians. This product is one of the supply-managed commodities in Canada, but unlike other commodities, where food safety risks are extensively explored and reported, information on the prevalence of enteric organisms (e.g., Salmonella, Campylobacter) and antimicrobial resistance (AMR) in layers in Canada are limited. This study was conducted to determine the prevalence of select bacteria and the associated AMR patterns in layer flocks using 2 sample matrices. Farms were located within FoodNet Canada and the Canadian Integrated Program for Antimicrobial Resistance Surveillance sentinel sites (SS). Fecal samples (Ontario: ONSS1a, ONSS1b) and environmental sponge swabs (British Columbia: BCSS2a) were collected. Salmonella prevalence was 29% and 8% in ONSS1a and ONSS1b, respectively, and 7% in BCSS2a. S. Kentucky and S. Livingstone were the most frequently isolated serovars and no S. Enteritidis was detected. Campylobacter was not detected in the BC sponge swabs but was isolated from 89% and 53% of Ontario fecal samples (ONSS1a and ONSS1b, respectively). Seven C. jejuni from Ontario were ciprofloxacin-resistant. Escherichia coli prevalence was high in both sample types (98%). Overall, tetracycline resistance among E. coli ranged from 26% to 69%. Resistance to ceftiofur (n = 2 isolates) and gentamicin (n = 2) was relatively low. There were diverse resistance patterns (excludes susceptible isolates) observed among E. coli in Ontario (10 patterns) and British Columbia (14 patterns). This study revealed that fecal samples are more informative for farm-level monitoring of pathogen and AMR prevalence. Without further validation, sponge swabs are limited in their utility for Campylobacter detection and thus, for public health surveillance. 相似文献
99.
Ferguson SM Brasnjo G Hayashi M Wölfel M Collesi C Giovedi S Raimondi A Gong LW Ariel P Paradise S O'toole E Flavell R Cremona O Miesenböck G Ryan TA De Camilli P 《Science (New York, N.Y.)》2007,316(5824):570-574
Dynamin 1 is a neuron-specific guanosine triphosphatase thought to be critically required for the fission reaction of synaptic vesicle endocytosis. Unexpectedly, mice lacking dynamin 1 were able to form functional synapses, even though their postnatal viability was limited. However, during spontaneous network activity, branched, tubular plasma membrane invaginations accumulated, capped by clathrin-coated pits, in synapses of dynamin 1-knockout mice. Synaptic vesicle endocytosis was severely impaired during strong exogenous stimulation but resumed efficiently when the stimulus was terminated. Thus, dynamin 1-independent mechanisms can support limited synaptic vesicle endocytosis, but dynamin 1 is needed during high levels of neuronal activity. 相似文献
100.
N. Richard Knowles W. M. Iritani L. D. Weller 《American Journal of Potato Research》1985,62(6):289-300
Plant growth from Russet Burbank seed-tubers of different ages was compared as affected by source of meristematic tissue (apical or basal) and NAA treatment. In controlled environment studies, single-eye seedcores from 17-mo-old seed-tubers produced twice as many shoots compared to those from 5-and 29-mo-old seed-tubers. Seed-tuber age and shoot dry weight/core were inversely related. Dry weight/shoot decreased as age increased to 17 mo and then remained unchanged from 17 to 29 mo. Apical meristems lost growth potential faster than basal meristems as age advanced. Meristem physiological age apparently depends on relative position of the buds as well as on the chronological age of the seed-tuber. NAA (10–40 mg/l) decreased the number of shoots/core, had no effect on shoot dry weight/core but increased the dry weight/shoot when applied to seedcores from 20-mo-old seed-tubers. On seedcores from 8-mo-old seed-tubers. NAA decreased the shoot dry weight (g/core, g/shoot). In field studies, NAA (100 mg/l) increased the total tuber yield of plants grown from seedpieces cut from 8-and 20-mo-old seed-tubers. These increases were manifested in age-specific effects on tuber grade. The effects of seedtuber age on plant growth and tuber yield were partially overcome by seedpiece treatment with NAA. 相似文献