Mechanical comparison of 3 different clamp and 2 different rod types of a new veterinary internal fixation system, 4.5/5.5-mm VetFix

OBJECTIVE: To compare the gripping force of a standard clamp with click-on and T clamps of the 4.5/5.5-mm VetFix (AO Research Institute, Davos, Switzerland), mounted on smooth and roughened rods. STUDY DESIGN: In vitro mechanical study. METHODS: Rods were mounted on a clamp-holding device. Each clamp type was tested on a materials-testing machine in groups of 6 specimens for torsional and axial load stability on smooth and roughened rods (total, 72 specimens). The variable evaluated was the discontinuity point (DP) as a measure for gliding resistance of the clamp on the rod. Means (+/-SD) were calculated for all groups separately. For statistical comparison, a multiple regression model was fitted with level of significance set at P =.05. RESULTS: The direction of torsion had a significant effect on DP for both asymmetric clamps (standard, click-on). In torsion and axial load, the roughened rod had significantly higher DP levels than the smooth rod for the standard clamp. Rods differed only in axial load for the T clamp, again favoring the roughened rod. The click-on clamp did not reach 10% of the values achieved by the other clamps, independent from the rods and loading conditions. Compared with the T clamp, standard clamp DP values were significantly higher in torsion on the roughened rod as well as in axial load on both rod types. CONCLUSIONS: The roughened rod with a standard clamp was mechanically superior. For the T clamp, the small loss of gripping force may be compensated for by the possibility of inserting 2 more screws. CLINICAL RELEVANCE: The roughened rod may enhance the bending, compressive, and torsional properties of the 4.5/5.5-mm VetFix system by reducing the risk of clamp gliding on the rod. The standard clamp is the clamp of choice, except for the metaphyseal or epiphyseal region, at which use of a T clamp may be indicated to increase the number of screws inserted at the end of the rod.     

Genome scan for the possibility of identifying candidate resistance genes for goat lentiviral infections in the Italian Garfagnina goat breed

Tropical Animal Health and Production - Small ruminant lentiviruses (SRLVs) are a heterogeneous group of viruses of sheep, goat, and wild ruminants responsible of lifelong persistent infection...     

Adjuvant medical therapy provides no therapeutic benefit in the treatment of dogs with low‐grade mast cell tumours and early nodal metastasis undergoing surgery

Lymph node (LN) metastasis is a negative prognostic factor in dogs with cutaneous mast cell tumours (cMCTs). While elective lymphadenectomy of metastatic LNs improves outcome, the benefit of adjuvant medical therapy in dogs with early metastatic (HN2) LNs is debated. The aim of this retrospective multicentre study was to evaluate the therapeutic benefit of adjuvant medical therapy following surgical removal of the primary low‐grade cMCT (Patnaik grade 1‐2 and Kiupel low‐grade) and lymphadenectomy of HN2 LNs by analysing survival rates and patterns of recurrence. Seventy‐three dogs were included: 42 received adjuvant medical treatment (chemotherapy and/or kinase inhibitors), and 31 did not. The median follow‐up time for medically treated dogs was 619 days: two experienced local recurrence, three nodal relapse and four distant relapse. For dogs undergoing surgery only, the median follow‐up time was 545 days. None of them experienced local recurrence, nodal, or distant relapse. Time to progression was significantly shorter in dogs receiving adjuvant medical treatment (P = .021). A similar tendency was observed for overall survival (P = .056). The current study shows that dogs with low‐grade cMCTs, that undergo surgical excision of the primary tumour and elective lymphadenectomy of the HN2 regional LN harbour a good prognosis. The use of adjuvant medical treatment in these dogs does not seem to provide any benefit in terms of progression and survival.     

Differential expression of microRNA-206 in skeletal muscle of female Piedmontese and Friesian cattle

The double-muscle phenotype is an inherited condition in Piedmontese cattle traced to a point mutation in the myostatin gene. To investigate the potential role of muscle-specific miRNAs in determining muscle development in cattle, this study examined the patterns of expression of microRNAs (miRNA-1) and miRNA-206 in Piedmontese and Friesian cattle according to phenotype and sex. There were no significant differences in miRNA-1 expression between different muscle phenotypes, sexes or breeds, whereas there was significantly higher expression of miRNA-206 in female Piedmontese compared with female Friesian cattle.     

Sensitivity to strobilurin fungicides of Italian Venturia inaequalis populations with different origin and scab control

BACKGROUND: Venturia inaequalis (Cooke) Winter with reduced sensitivity to strobilurins has been reported in several countries, including Italy. This study aimed to characterise the sensitivity to strobilurins of three different types of V. inaequalis population: (a) wild types; (b) from commercial orchards satisfactorily managed with strobilurins; (c) from an experimental orchard with control failures by trifloxystrobin and kresoxim‐methyl. In vitro sensitivity tests included antigerminative activity on population conidia and mycelial growth inhibition on monoconidial isolates. Cleaved amplified polymorphic sequence (CAPS) analysis was used for the detection of G143A substitution. RESULTS: Wild‐type populations showed EC₅₀ values lower than 0.031 mg L^?1, while those of orchards with good performance by strobilurins presented EC₅₀ values never higher than 0.063 mg L^?1. Samples with scab control failures showed a strongly reduced population sensitivity. Similar differences were confirmed in monoconidial isolates. The G143A substitution was always detected in low‐sensitivity populations, only sometimes in well‐controlled populations and generally not in wild types. CONCLUSIONS: In vitro sensitivity assays were able to discriminate the three population types with different scab management, while the qualitative PCR analysis (CAPS) was only partially reliable. High sensitivity differences among V. inaequalis populations with good and poor field control by strobilurins were observed. Copyright © 2011 Society of Chemical Industry     

Europe's terrestrial biosphere absorbs 7 to 12% of European anthropogenic CO2 emissions

Most inverse atmospheric models report considerable uptake of carbon dioxide in Europe's terrestrial biosphere. In contrast, carbon stocks in terrestrial ecosystems increase at a much smaller rate, with carbon gains in forests and grassland soils almost being offset by carbon losses from cropland and peat soils. Accounting for non-carbon dioxide carbon transfers that are not detected by the atmospheric models and for carbon dioxide fluxes bypassing the ecosystem carbon stocks considerably reduces the gap between the small carbon-stock changes and the larger carbon dioxide uptake estimated by atmospheric models. The remaining difference could be because of missing components in the stock-change approach, as well as the large uncertainty in both methods. With the use of the corrected atmosphere- and land-based estimates as a dual constraint, we estimate a net carbon sink between 135 and 205 teragrams per year in Europe's terrestrial biosphere, the equivalent of 7 to 12% of the 1995 anthropogenic carbon emissions.     

SEASONAL CHANGES IN LEAF NITROGEN OF OLIVE TREES GROWN UNDER DIFFERENT IRRIGATION REGIMES AND CROP LEVEL

An experiment was conducted over 12 months using field-grown olive trees (Olea europaea) to assess the combined effect of soil water availability and fruit number on seasonal changes in leaf nitrogen (N) concentration. Three irrigation regimes were established and three trees per irrigation treatment were thinned to reduce their yield to about half that of unthinned trees. The N concentration of fully-expanded leaves from either the current-year growth or one-year old part of fruiting shoots was determined every two months. Nitrogen concentration was higher in current-year leaves than in one-year old ones at most sampling dates. Maximum values of leaf N were measured in spring, minimum values in August. Leaf N concentrations were positively correlated with leaf water potential during fruit development. This relationship was weak at the onset of rapid oil accumulation in August and became more evident at harvest. There was no correlation between leaf N and crop level.     

Sound discrimination of two sympatric,threatened fish species allows for their in situ mapping

1. Human impacts on marine ecosystems are increasing and the number of fish species listed in the Red List is constantly growing. In the Mediterranean Sea, seven of the 10 bony fishes defined as Threatened by the IUCN are known to be vocal, including the target species of this study: the shi drum (Umbrina cirrosa Linnaeus, 1758) and the brown meagre (Sciaena umbra Linnaeus, 1758). As a result, non-invasive passive acoustic monitoring (PAM) can be used to pinpoint their distribution at sea. However, for PAM to be effective, reliable acoustic discrimination is required because the sounds they emit during reproduction are remarkably similar (i.e. short broadband pulsed sounds).
2. The shi drum and the brown meagre are closely related, elusive, vocal sciaenids, partially overlapping in their ecological niche. During summer 2019, three PAM surveys were conducted along the tidal inlets of the Venice lagoon (Italy). Here, the calls of both species have been recognized according to their temporal features: shi drum sounds were made up of a lower number of longer pulses with a different envelope, repeated at a lower rate than those of the brown meagre. Further, shi drum and brown meagre sounds of different origins (aquaculture and semi-natural conditions) were analysed and compared with those collected during the survey of the study area in order to validate the field collected data.
3. Call discrimination allowed for a fine-scale species mapping, showing a partially overlapping distribution of the two species along the inlets. This is the first case in which two sciaenids have been documented to share their reproductive habitat in the Mediterranean Sea.
4. This study demonstrates that it is feasible to acoustically monitor the target species even in those parts of the Mediterranean Sea where they co-exist. This, in its turn, could provide managers with the required data for effective conservation measures to be implemented.

     

Encapsulation of Carlina acaulis essential oil and carlina oxide to develop long-lasting mosquito larvicides: microemulsions versus nanoemulsions

Journal of Pest Science - Carlina acaulis root essential oil (EO) is one of the most potent mosquito larvicides (LC50?<?2 ppm). This EO is mainly composed of carlina...