Intra‐ovarian dynamic blood flow in pseudopregnant rabbits during prostaglandin F2α‐induced luteolysis

In the present study, we evaluated the dynamic changes of intra‐ovarian blood flow, by real‐time colour‐coded and pulsed Doppler ultrasonography, as well as the immunopresence of prostaglandin F2α (PGF2α) receptor (FP) and peripheral plasma progesterone concentrations in pseudopregnant rabbit after PGF2α treatments at either early‐ (4 days) and mid‐luteal (9 days) stages. During the pre‐treatment observation interval of one hour, the ovarian blood flows showed a fluctuating pattern. Independently of luteal stage, PGF2α administration caused a fourfold decline in the blood flow within 40 min that was followed 50 min later by a reactive hyperaemia that lasted several hours, while the resistive index showed an opposite trend. Twenty‐four hour later, the blood flow was one half that measured before PGF2α injection. At day 4 of pseudopregnancy, PGF2α did not affect peripheral plasma progesterone concentrations, but at day 9, it caused functional luteolysis as progesterone levels declined 6 hr later to reach basal values after 24 hr. The changes in the ovarian blood flows of pseudopregnant rabbits receiving PGF2α were accompanied by simultaneous changes in the resistance index. This biphasic response in the blood flow and vascular resistances likely reflects reactive hyperaemia following vasoconstriction. By immunohistochemistry, strong positive immune reaction for FP was detected in the cytoplasm of endothelial cells of ovarian arteries, veins and capillaries. In conclusion, these results suggest that PGF2α could acutely regulate the ovarian blood flow of pseudopregnant rabbits, even if there is no evidence of a blood flow reduction anticipating luteolysis.     

Growth and mineral absorption in maize seedlings as affected by increasing NaCl concentrations

The influence of NaCl solutions of decreasing osmotic potentials (¥π = ‐0.44 and ‐0.88 MPa) on seedling growth and on the concentration of the most important macro‐ and micro‐nutrients in the shoots and roots of maize (Zea mays L., cv. Summer II) grown in Hoagland's solution in a growth chamber was studied. Salt stress was imposed on six‐day‐old seedlings for a three day period. Increasing NaCl concentrations induced a reduction in the leaf water potential and a significant decrease in the length and dry weight of the shoots, whereas these two parameters decreased in the roots only at the lowest osmotic potential.

Although the absorption and accumulation of nutrients upon salt stress differed in the two treatments depending on the plant tissue and nutrient, almost all of the macronutrients decreased in the roots and shoots, showing the lowest values at ¥π = ‐0.88 MPa. Sodium and Cl increased continuously, much more in the roots than in the shoots.

A different response was seen in the shoots and roots in terms of micronutrients. In the roots, almost all of the ions reached their maximum concentrations at ¥π = ‐0.88 MPa; whereas in the shoots, they decreased at ¥π = ‐0.44 MPa without undergoing any further decrease at the lowest osmotic potential.     

Occurrence of anthelmintic resistant equine cyathostome populations in central and southern Italy

In the present survey, 276 horses bred on 16 farms located in central and southern Italy were investigated for the presence of drug resistant cyathostomes by a Fecal Egg Count Reduction Test (FECRT). Sixteen to 20 animals were selected on each farm and randomly assigned to one of four equally sized treatment groups. Groups were treated with fenbendazole, pyrantel pamoate, ivermectin or moxidectin. Resistance to fenbendazole was declared on six farms (37.5%) and suspected in two farms (12.5%), with FECR values ranging from 41% to 88.3%. Resistance to pyrantel was found in two farms (12.5%) and was suspected in one case (6.2%), with FECR values ranging from 43% to 85.4%. Macrocyclic lactones remained effective on all farms. Only cyathostome third stage larvae (L3) were found in fecal cultures after treatment. This paper reports the first wide survey conducted in Italy for anthelmintic resistance in equine cyathostomes. The results indicate that multiple drug resistant equine cyathostomes are present in the central and southern regions of Italy. These data call for a geographically and numerically broader investigation of horse farms in all regions and for the development and implementation -among veterinarians, owners and managers of a plan to reduce the expansion of these anthelmintic resistant populations and control these important parasites.     

Illegal ingegno fishery and conservation of deep red coral banks in the Sicily Channel (Mediterranean Sea)

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Development and study of a maize cultivar rich in anthocyanins: coloured polenta,a new functional food

Among the phytonutrients, anthocyanins have been extensively studied because of their antioxidant power, the characteristic supposedly responsible for their capacity for chronic disease prevention. Anthocyanins can also be synthesized in maize even though in Europe the colourless varieties have always been preferred. The aim of this study was to develop and characterize a new polenta variety of maize rich in anthocyanins, bred by a recurrent selection scheme, to increase the antioxidant power of this food. The recurrent selection was based on the anthocyanin content and other specific traits of the kernel. The coloured polenta obtained was analysed by TLC (Thin Layer Chromatography) analysis and DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) radical scavenging ability, before and after cooking. The results obtained showed that even though cooking reduced the anthocyanin content by about 22%, the remaining anthocyanins exhibited twofolds higher antioxidant capacity, expressed as ARP (antiradical power) using acetone/water extraction buffer in DPPH assay, compared to the colourless control. Furthermore, our data showed that the anthocyanin content did not alter the taste of the coloured polenta.     

Interactions of organic and inorganic chromium species with Ca-polygalacturonate

This study investigated the interactions of CrIII and CrVI present as free metal ions or as organic CrIII (Cr-L) with a Ca-polygalacturonate (Ca-PG) network. Ca-PG networks were treated with solutions of chromium (CrIII and CrVI) at different concentrations at three different pH values. The Cr-L complex were obtained by water extraction of hydrolysed leather. The CrVI had almost no interaction with the Ca-PG networks, whereas CrIII as a free metallic ion was strongly retained by the network. The calculated Langmuir adsorption isotherms showed a twofold increase in maximum CrIII adsorption but a decrease in the affinity of the metal with the Ca-PG networks. This would indicate that the different chromium species, i.e. CrIII and its hydrolytic species, interact in a different way with the polygalacturonate chains. In addition, thermal analysis showed that CrIII sorption modified the structure of the network. These modifications were reduced, and thermal stability increased if Ca-PG adsorbed Cr-L or L. Furthermore, the treatment of Ca-PG with hydrolysed leather water extract-reduced CrIII sorption by 94%. The hydrolysed leather fraction immobilized onto the Ca-PG networks probably consisted of peptides and could therefore represent an important source of organic N.     

Chemical compounds released from five different woods used to make barrels for aging wines and spirits: volatile compounds and polyphenols

Aging wine and spirits in wooden barrels is an industrial process used to stabilize color and improve limpidity; many compounds are released from the wood and enrich the sensorial characteristics of the product. The main wood species used for making barrels is oak, but in particular cases also acacia, chestnut, cherry and mulberry. In this work, polyphenols contained in the extracts of these wood species obtained by solutions of 50% hydroalcohol as well as a model wine were studied and compared with the extracts from oak. The hydroalcoholic extracts of chestnut and mulberry had higher total polyphenols, followed by cherry, acacia and oak, respectively. The oak model wine extract had the highest percentage of polyphenols extractable by the wine, followed by chestnut, acacia, cherry and mulberry, respectively. Chestnut extracts had the highest percentage of oxidizable compounds, followed by acacia, oak, mulberry and cherry. The GC/MS–EI profile of 50% hydroalcoholic extracts revealed as principal volatiles several benzene compounds containing a guaiacol residue, and high contents of C₆–C₁₈ fatty acids. To our knowledge, this is the first study reporting on polyphenolic and complete volatile compounds characterization of these woods for oenological purposes.